BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0018.p (578 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 1194 0.0 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 792 0.0 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 433 e-122 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 242 6e-65 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 175 1e-44 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 1194 bits (3088), Expect = 0.0, Method: Compositional matrix adjust. Identities = 577/578 (99%), Positives = 578/578 (100%) Query: 1 MTDDTKKSTRRSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNS 60 MTDDTKKSTRRSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNS Sbjct: 1 MTDDTKKSTRRSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNS 60 Query: 61 HFRALAEQVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGV 120 HFRALAEQVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGV Sbjct: 61 HFRALAEQVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGV 120 Query: 121 VLLMGCDKTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKM 180 VLLMGCDKTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKM Sbjct: 121 VLLMGCDKTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKM 180 Query: 181 TQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAG 240 TQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAG Sbjct: 181 TQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAG 240 Query: 241 RRIVQMVEEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDD 300 RRIVQMVEEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDD Sbjct: 241 RRIVQMVEEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDD 300 Query: 301 IGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQLESVIDKTALTANGKTLWENCQDAPN 360 IGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQLESVIDKTALTANGKTLWENCQDAPN Sbjct: 301 IGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQLESVIDKTALTANGKTLWENCQDAPN 360 Query: 361 WNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHK 420 WNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHK Sbjct: 361 WNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHK 420 Query: 421 RIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTA 480 RIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTA Sbjct: 421 RIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTA 480 Query: 481 YGTVVLHVSPEAAAGGPLALVQNGDFIELDVEARKLHLDVSDEELARRRAQWQKPELPPQ 540 YGTVVLHVSPEAAAGGPLA+VQNGDFIELDVEARKLHLDVSDEELARRRAQWQKPELPPQ Sbjct: 481 YGTVVLHVSPEAAAGGPLAVVQNGDFIELDVEARKLHLDVSDEELARRRAQWQKPELPPQ 540 Query: 541 MQRGWVKLYVDHVQQANQGADLDFLVGKSGPYVPKDNH 578 MQRGWVKLYVDHVQQANQGADLDFLVGKSGPYVPKDNH Sbjct: 541 MQRGWVKLYVDHVQQANQGADLDFLVGKSGPYVPKDNH 578 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 792 bits (2046), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/585 (66%), Positives = 467/585 (79%), Gaps = 7/585 (1%) Query: 1 MTDDTKKSTR--RSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPC 58 M+ D K +R RS WFG D++GF+YRSW+KN+GIP +F G+PVIGICNT+SELTPC Sbjct: 1 MSSDKKDKSRTLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPC 60 Query: 59 NSHFRALAEQVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLD 118 N+HFR +AE V+ GI EAGGFP+EFPV S GE+ LRPTAML RNLASMDVEESIR NP+D Sbjct: 61 NAHFRKIAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPID 120 Query: 119 GVVLLMGCDKTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAG 178 VVLL GCDKTTPALLMGAAS DVP I V+GGPML+GK++GR++GSGT VWQ+SE V+AG Sbjct: 121 AVVLLTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAG 180 Query: 179 KMTQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARN 238 ++T +F AE+ M RS G C TMGTASTMA M E+LGV LPHNAAIPAVDARR VLA Sbjct: 181 EITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHL 240 Query: 239 AGRRIVQMVEEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDW 298 +G RIV MV E L LSKILTR+AFENAIR NAAIGGSTNAVIHL AIAGRIG+ L+L+DW Sbjct: 241 SGMRIVDMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDW 300 Query: 299 DDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQL---ESVIDKTALTANGKTLWENC 355 IG+ P ++DLQPSG++LME+FYYAGGLPAV+R+L + + K ALT NG+T+W+N Sbjct: 301 TRIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNV 360 Query: 356 QDAPNWNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENS 415 +DAP +N+EV+R KP E GI IL+GNLAP GAV+KPSAATP L+KHRGRAVVFE+ Sbjct: 361 KDAPIYNDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDF 420 Query: 416 DDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDAR 475 + +RI+D +LDVD +CVLV+KN GPKGYPGMAE GNM LPPK+L G+ DMVR+SDAR Sbjct: 421 NHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDAR 480 Query: 476 MSGTAYGTVVLHVSPEAAAGGPLALVQNGDFIELDVEARKLHLDVSDEELARRRAQWQK- 534 MSGTAYGTV+LHV+PEAAAGGPL +VQ+GDFIELD A KL LD+S+EE+ RR K Sbjct: 481 MSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKV 540 Query: 535 -PELPPQMQRGWVKLYVDHVQQANQGADLDFLVGKSGPYVPKDNH 578 E P+M G+ LYVD V QA++G D DFLVG G VPK +H Sbjct: 541 LAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAAVPKHSH 585 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 433 bits (1114), Expect = e-122, Method: Compositional matrix adjust. Identities = 245/586 (41%), Positives = 353/586 (60%), Gaps = 21/586 (3%) Query: 11 RSQAWFGRQDRDGF--IYRSWVKNRGIPHDQF-DGRPVIGICNTYSELTPCNSHFRALAE 67 RSQ WF D +Y N GI ++ GRP+IGI + S+++PCN LA+ Sbjct: 12 RSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHLELAK 71 Query: 68 QVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGVVLLMGCD 127 +V+ GI +AGG P+EFP+ + E RPTA + RNLA + + E + P+D VVL GCD Sbjct: 72 RVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAVVLTTGCD 131 Query: 128 KTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKMTQEEFFE 187 KTTP+ +M AA+VD+P I +SGGPML G G +GSG+ +W+ + + AG + E+F E Sbjct: 132 KTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEKFLE 191 Query: 188 AESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMV 247 + S GHC TMGTASTM +M EALG+ L +AIPA R +A GRRIV M Sbjct: 192 IAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA 251 Query: 248 EEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDDIGQQLPC 307 EDL S ILTR AF +AI VNAAIGGSTNA H++A+A G++L +DW G +P Sbjct: 252 YEDLRPSAILTRDAFLDAIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDVPL 311 Query: 308 LLDLQPSGKFLMEDFYYAGGLPAVIRQLESV--IDKTALTANGKTLWENCQDAPNWNEEV 365 LL++QP+GK+L E F+ AGG+PA++ +L+ + +TA GKT+ EN Q + + E+ Sbjct: 312 LLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQGRASNDREM 371 Query: 366 IRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKH-----------RGRAVVFEN 414 I F P +E AG +LKGNL D A++K S + + RAVVF+ Sbjct: 372 IYPFAAPLRERAGFLVLKGNLF-DFAIMKTSVISETFRERYLSTPGQENIFECRAVVFDG 430 Query: 415 SDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDA 474 SDD H RI+D L +DE +L ++ GP G+PG AE NM P ++++G++ + + D Sbjct: 431 SDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVSTLPTLGDG 490 Query: 475 RMSGTAYGTVVLHVSPEAAAGGPLALVQNGDFIELDVEARKLHLDVSDEELARRRAQWQK 534 R SGT+ +L+ SPE+A GG LA +++GD + +D+ + ++ VS+EELARR+++ Sbjct: 491 RQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEELARRKSE-GI 549 Query: 535 PELPPQMQRGWVKLYVDHVQQANQGADLDFLVGKSG--PYVPKDNH 578 P +PP Q W ++Y V Q GA ++ + G +P+ NH Sbjct: 550 PPVPPS-QTPWQEIYRSTVGQLETGACMELALKYQGVAQTLPRHNH 594 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 242 bits (618), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 184/622 (29%), Positives = 313/622 (50%), Gaps = 81/622 (13%) Query: 13 QAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFRALAEQVKIG 72 +++ Q R+ R+ + G+ F +P+I + N++++ P + H + L + V Sbjct: 5 RSYTTTQGRNMAGARALWRATGMKDGDFT-KPIIAVVNSFTQFVPGHVHLKDLGQLVARE 63 Query: 73 IWEAGGFPLEFPVMSLGETLLRPTA-MLY----RNLASMDVEESIRANPLDGVVLLMGCD 127 I AGG EF +++ ++ A MLY R+L + VE + A+ D +V + CD Sbjct: 64 IEAAGGVAKEFNTIAVDDSGTHGDARMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCD 123 Query: 128 KTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMS---EDVRAG--KMTQ 182 K TP +LM A +++P + VSGGPM +GK + + + V ++ ++AG ++ Sbjct: 124 KITPGMLMAAMRLNIPVVFVSGGPMEAGKVI-KTVNTDKKVIKLDLVDAMIQAGDKNISD 182 Query: 183 EEFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRR 242 + E E + G C M TA++M + E LG+ LP N I A A R L AGR Sbjct: 183 ADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAGRL 242 Query: 243 IVQMV----EED---LVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDL 295 IV++ E+D ++ I T++ +ENA+ ++ ++GGSTN V+HLLA A ++ + Sbjct: 243 IVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEFTM 302 Query: 296 KDWDDIGQQLPCLLDLQP-SGKFLMEDFYYAGGLPAVIRQL--ESVIDKTALTANGKTLW 352 D D I +++PCL + P + K+ +ED + AGG+ +++ +L ++D + T + KTL Sbjct: 303 ADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKTLG 362 Query: 353 ENCQD-----------------APN-------WNEE-------------VIRSFDKPFKE 375 E + AP +++E IR + + + Sbjct: 363 EAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAYSQ 422 Query: 376 AAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVL 435 G+A+L GN+A G ++K + ++LK G A VFE+ D + I + + + V+ Sbjct: 423 DGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDKVKAGD--VV 480 Query: 436 VLKNCGPKGYPGMAEAGNMPLPPKILR-KGITDMVRV-SDARMSGTAYGTVVLHVSPEAA 493 +++ GPKG PGM E M P ++ KG+ + +D R SG + G V+ H SPEAA Sbjct: 481 IVRYEGPKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAA 537 Query: 494 AGGPLALVQNGDFIELDVEARKLHLDVSDEELARRR--------AQWQKPELPPQMQR-- 543 GG + LV+ GD I++D+ R ++L +S ++LA+RR A WQ P QR Sbjct: 538 EGGAIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQ----PGNRQRVV 593 Query: 544 -GWVKLYVDHVQQANQGADLDF 564 ++ Y A++GA D Sbjct: 594 SQALQAYAALTTSADRGAVRDL 615 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 175 bits (443), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 157/523 (30%), Positives = 239/523 (45%), Gaps = 48/523 (9%) Query: 41 DGRPVIGICNTYSELTPCNSHFRALAEQVKIGIWEAGG---FPLEFPVMSLGETLLRP-- 95 D P + I + Y+++ + F + +K EAG F P M G T + Sbjct: 65 DKAPSLAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQGQAGM 124 Query: 96 -TAMLYRNLASMDVEESIRANPLDGVVLLMGCDKTTPALLMGAASV-DVPTIGVSGGPML 153 ++ R+ +M ++ N D + L CDK P LL+GA +P + V GPM Sbjct: 125 ELSLFSRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMT 184 Query: 154 SGKYRGRELGSGTGVWQMSEDVRAGKMTQEEFFEAESCMHRSHGHCMTMGTASTMASMVE 213 SG S + ++ + AGK+ + E EAES + G C GTA++ ++E Sbjct: 185 SGM-------SNSDKVKIRQLYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLME 237 Query: 214 ALGVGLPHNAAIPAVDARRNVLARNAGR---RIVQMVEEDLVLSKILTRQAFENAIRVNA 270 A+G+ LP A I R+ L A + +I + + + +++ ++ NAI Sbjct: 238 AMGLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQYTPVGRMIDEKSIVNAIVALH 297 Query: 271 AIGGSTNAVIHLLAIAGRIGIKLDLKDWDDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPA 330 A GGSTN +HL+AIA GI +D D+D + +P + + P+G + F+ AGG Sbjct: 298 ATGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGF 357 Query: 331 VIRQL--ESVIDKTALTANGKTLWENCQ-------------------DAP--NWNEEVIR 367 V+R+L ++ T G+ L ++C+ DAP + +E V+ Sbjct: 358 VLRELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPAQSGDEGVLG 417 Query: 368 SFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENL 427 + KPF G+ +L+GNL AVIK SA P AVVF + + L Sbjct: 418 TVAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGQL 475 Query: 428 DVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTAYGTV--V 485 D D V+ + GP+ GM E + IL+ + V+D RMSG A G V Sbjct: 476 DRDFVAVITFQ--GPRAN-GMPELHALTPALGILQDKGRHVALVTDGRMSG-ASGKVPAA 531 Query: 486 LHVSPEAAAGGPLALVQNGDFIELDVEARKLHLDVSDEELARR 528 +HV+PE GGPL V++GD I LD EA L V E A R Sbjct: 532 IHVTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAKVDATEWAAR 574 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,958,228 Number of extensions: 87386 Number of successful extensions: 245 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 6 Length of query: 578 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 488 Effective length of database: 1,193,720 Effective search space: 582535360 Effective search space used: 582535360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)