BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0014.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312636|ref|YP_003776728.1| sugar ABC transporter permease ... 591 e-170 gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease ... 102 5e-23 gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC tra... 95 6e-21 gi|300312388|ref|YP_003776480.1| sugar ABC transporter permease ... 93 3e-20 gi|300311926|ref|YP_003776018.1| glycerol-3-phosphate ABC transp... 80 1e-16 >gi|300312636|ref|YP_003776728.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 299 Score = 591 bits (1524), Expect = e-170, Method: Compositional matrix adjust. Identities = 299/299 (100%), Positives = 299/299 (100%) Query: 1 MLSRFLNNRNVLGMLFMAPAVILLVVFLTYPLGLGIWLGFTDTKIGGEGSFIGLDNFTYL 60 MLSRFLNNRNVLGMLFMAPAVILLVVFLTYPLGLGIWLGFTDTKIGGEGSFIGLDNFTYL Sbjct: 1 MLSRFLNNRNVLGMLFMAPAVILLVVFLTYPLGLGIWLGFTDTKIGGEGSFIGLDNFTYL 60 Query: 61 AGDSLAQLSLFNTVFYTVSASILKFMLGLWLAILLNKNVPLKTFFRAIVLLPWIVPTALS 120 AGDSLAQLSLFNTVFYTVSASILKFMLGLWLAILLNKNVPLKTFFRAIVLLPWIVPTALS Sbjct: 61 AGDSLAQLSLFNTVFYTVSASILKFMLGLWLAILLNKNVPLKTFFRAIVLLPWIVPTALS 120 Query: 121 ALAFWWLYDAQFSVISWALHKMGLIDRYIDFLGDPWNARWSTVFANVWRGIPFVAISLLA 180 ALAFWWLYDAQFSVISWALHKMGLIDRYIDFLGDPWNARWSTVFANVWRGIPFVAISLLA Sbjct: 121 ALAFWWLYDAQFSVISWALHKMGLIDRYIDFLGDPWNARWSTVFANVWRGIPFVAISLLA 180 Query: 181 GLQTISPSLYEAAAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTDFQLIYVLTRG 240 GLQTISPSLYEAAAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTDFQLIYVLTRG Sbjct: 181 GLQTISPSLYEAAAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTDFQLIYVLTRG 240 Query: 241 GPLNATHLMATLSFQRAIPGGALGEGAALATYMIPFLLAAIMFSYFGLQRRGWQQGGDK 299 GPLNATHLMATLSFQRAIPGGALGEGAALATYMIPFLLAAIMFSYFGLQRRGWQQGGDK Sbjct: 241 GPLNATHLMATLSFQRAIPGGALGEGAALATYMIPFLLAAIMFSYFGLQRRGWQQGGDK 299 >gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 311 Score = 102 bits (253), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 76/273 (27%), Positives = 130/273 (47%), Gaps = 15/273 (5%) Query: 16 FMAPAVILLVVFLTYPLGLGIWLGFTDTKIGGEGSFIGLDNFTYLAGDSLAQLSLFNTVF 75 F+APA ++ +V++ YP+ I+L F E F+GL N+ L +L N V Sbjct: 41 FLAPACVMTLVYVLYPILYTIYLSFFSWDGMTEPRFVGLANYVELFHAPTFYTALKNNVL 100 Query: 76 YTVSASILKFML----GLWLAILLNKNVPLKTFFRAIVLLPWIVPTALSALAFWWLYDAQ 131 + +L F+L GL +A+ LN+ V +++ P+++ + L + W YD Sbjct: 101 W-----LLMFLLAPPMGLAIALYLNQKVIGMRVVKSLFFAPFVLSGVVVGLMYSWFYDPS 155 Query: 132 FSVISWALHKMGLIDRYIDFLGDPWNARWSTVFANVWRGIPFVAISLLAGLQTISPSLYE 191 F ++S L + + LGD A + +FA +W + I L GL ++ E Sbjct: 156 FGLLSL------LFGQGVPVLGDARYATFGIIFAALWPQTAYCMILFLTGLTALNHDQVE 209 Query: 192 AAAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTDFQLIYVLTRGGPLNATHLMAT 251 AA ++GA W R+V LP L + S++ F LI V+T GGP ++ ++A Sbjct: 210 AARMEGAKGWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLISVMTSGGPYESSTVLAY 269 Query: 252 LSFQRAIPGGALGEGAALATYMIPFLLAAIMFS 284 ++ +AI G AA+A + +L I++ Sbjct: 270 YAYDQAIKYYRQGYSAAVAVTLFAIMLVYIVYQ 302 >gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 95.1 bits (235), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 76/278 (27%), Positives = 130/278 (46%), Gaps = 7/278 (2%) Query: 12 LGMLFMAPAVILLVVFLTYPLGLGIWLG-FTDTKIGGEGSFIGLDNFTYLAGDSLAQLSL 70 L L +AP +I+ ++F +P ++ GG F+ DNF L GD S Sbjct: 12 LPYLLVAPQIIITLLFFFWPAVQALYQSMLLQDAFGGYSEFVWFDNFKTLFGDPTYLESF 71 Query: 71 FNTVFYTVSASILKFMLGLWLAILLNKNVPLKTFFRAIVLLPWIVPTALSALAFWWLYDA 130 T ++ + L L LA+ ++ + ++ ++ P+ V + + + +L Sbjct: 72 RTTAIFSALVAFFGLALSLLLAVFADRVRRGASIYKTFLIWPYAVSPVVVGVLWMFLLSP 131 Query: 131 QFSVISWALHKMGLIDRYIDFLGDPWNARWSTVFANVWRGIPFVAISLLAGLQTISPSLY 190 ++S L +GL D++ + +A V A +W+ I + + LAGLQ+I SL Sbjct: 132 SLGILSHVLSWLGLP---WDYMINGTHAMILIVIAAIWKQISYNFLFFLAGLQSIPKSLI 188 Query: 191 EAAAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTD-FQLIYVLTRGGPLNATHLM 249 EAAAIDGA P ++F + PL++P + +V++ F D F ++ T GGP T ++ Sbjct: 189 EAAAIDGAGPVKRFFTIVFPLISPTTFFLFVVNVVYAFFDTFVIVDATTHGGPGKDTEIL 248 Query: 250 ATLSFQRAIPGGALGEGAALATYMIPF--LLAAIMFSY 285 F GG LG AA + ++ LL + F Y Sbjct: 249 VYKVFSDGFKGGDLGGSAAQSVVLMALVILLTVVQFKY 286 >gi|300312388|ref|YP_003776480.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 291 Score = 92.8 bits (229), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 75/241 (31%), Positives = 124/241 (51%), Gaps = 3/241 (1%) Query: 10 NVLGMLFMAPAVILLVVFLTYPLGLGIWLGFTDTKIGGEGS-FIGLDNFTYLAGDSLAQL 68 +VL L ++PA++LL+ F YP +W F T G S F+GL+N+ +A D + Sbjct: 7 SVLPWLLLSPAMVLLIAFTHYPALATLWHSFFSTPKGVRPSVFVGLENYRLMADDPVFWQ 66 Query: 69 SLFNTVFYTVSASILKFMLGLWLAILLNKNVPLKTFFRAIVLLPWIVPTALSALAFWWLY 128 +L N +++ + L L + +A+ +N+ + + R P ++P A + + Y Sbjct: 67 ALCNNLWFALGTIPLAIALAILMALWVNEKLAGRGVLRLAYFTPTVLPMIAVANIWLFFY 126 Query: 129 DAQFSVISWALHKMGLIDRYIDFLGDPWNARWSTVFANVWRGIPFVAISLLAGLQTISPS 188 Q+ ++ +GL ++LG+P + + VW+ F I LA LQ ISP Sbjct: 127 TPQYGLLEQITGALGLPSH--NWLGNPQTVLGALMVVAVWKEAGFFMIFYLAALQQISPV 184 Query: 189 LYEAAAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTDFQLIYVLTRGGPLNATHL 248 L EAAA++GA+ WQ F V PLL P V+ +++ F I V+T+GGP NA+ L Sbjct: 185 LAEAAALEGASRWQYFWRVQFPLLMPTTLFVLINALINAFRLVDHIVVMTKGGPDNASSL 244 Query: 249 M 249 + Sbjct: 245 L 245 >gi|300311926|ref|YP_003776018.1| glycerol-3-phosphate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 80.5 bits (197), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 81/297 (27%), Positives = 137/297 (46%), Gaps = 28/297 (9%) Query: 14 MLFMAPAVILLVVFLTYPLGLGIWLGFTDTKIGGEGS-FIGLDNFTYLAGDSLAQLSLFN 72 +L P ++++ +F +P I F + G GS F+GLDN+ + D SL Sbjct: 14 LLLATPQMLIVFLFFLWPAVKAIAWSFYLVRPFGNGSRFVGLDNYLRILTDDSFYASLRA 73 Query: 73 TVFYTVSASILKFMLGLWLAILLNKNVPLKTFFRAIVLLPWIVPTALSALAFWWLYDAQF 132 T+ +T +++L + L LA L +V K R+I + P+ V + + L + Sbjct: 74 TLVFTAGSTLLAVTIALALAACLELDVRAKRLLRSIFIWPYAVAGVVVGIVLKVLINPVT 133 Query: 133 SVIS---------WALHKMGLIDRYIDFLGDPWNARWSTVFANVWRGIPFVAISLLAGLQ 183 +++ WA H +G A + + A W +P I A LQ Sbjct: 134 GLLAFLNQLWPGVWAPHLIGA------------QAMLALIAAFAWTQVPLNFILFSAALQ 181 Query: 184 TISPSLYEAAAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTD-FQLIYVLTRGGP 242 I L AAAIDGA PW++F + LP++ P + + +V+ FT F L+ LT+GGP Sbjct: 182 RIPADLMGAAAIDGAGPWRRFIDIQLPMIAPALLLAGVINVIEAFTHGFGLVDALTQGGP 241 Query: 243 LNATHLMATLSFQRAIPGGALGEGAALATYMIPFLLAAIMFSYFGLQRRGWQQGGDK 299 +T ++A + G L +AL+ ++ F++A + LQ R ++QG + Sbjct: 242 GRSTAILAYQIYSDGFIGLDLSGSSALSVVLMLFIVALTL-----LQLRFFRQGAGR 293 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.459 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 960,468 Number of extensions: 40026 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 11 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)