BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0012.p (441 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein ... 911 0.0 gi|300313762|ref|YP_003777854.1| cytochrome c precursor protein ... 438 e-124 gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-de... 385 e-108 gi|300310719|ref|YP_003774811.1| cytochrome c protein [Herbaspir... 298 4e-82 gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herb... 282 5e-77 >gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 911 bits (2355), Expect = 0.0, Method: Compositional matrix adjust. Identities = 440/441 (99%), Positives = 440/441 (99%) Query: 1 MIKHYFIAACAAVMSLSALTAAAQSNPAAPSADQQLVQRGEYLAKAGDCVACHTAKGGKP 60 MIKHYFIAACAAVMSLSALTAAAQSNPAAPSADQQLVQRGEYLAKAGDCVACHTAKGGKP Sbjct: 1 MIKHYFIAACAAVMSLSALTAAAQSNPAAPSADQQLVQRGEYLAKAGDCVACHTAKGGKP 60 Query: 61 FAGGLAIATPIGTVYSSNITPDKENGIGNYSEEDFDRALRHGIRKDGASLYPAMPYPSYA 120 FAGGLAIATPIGTVYSSNITPDKENGIGNYSEEDFDRALRHGIRKDGASLYPAMPYPSYA Sbjct: 61 FAGGLAIATPIGTVYSSNITPDKENGIGNYSEEDFDRALRHGIRKDGASLYPAMPYPSYA 120 Query: 121 KVKPADVKALYAYFMHGVQADPAPNRGVDITWPLSMRWPLSIWRKVFAPAVAVDGPEDNS 180 KVKPADVKALYAYFMHGVQADPAPNRGVDITWPLSMRWPLSIWRKVFAPAVAVDGPEDNS Sbjct: 121 KVKPADVKALYAYFMHGVQADPAPNRGVDITWPLSMRWPLSIWRKVFAPAVAVDGPEDNS 180 Query: 181 PLVRGQYLVEGLGHCGACHTPRGVGMQEKALSNDSSQFLSGGVIDGYLANNLRGDARDGL 240 PLVRGQYLVEGLGHCGACHTPRGVGMQEKALSNDSSQFLSGGVIDGYLANNLRGD RDGL Sbjct: 181 PLVRGQYLVEGLGHCGACHTPRGVGMQEKALSNDSSQFLSGGVIDGYLANNLRGDGRDGL 240 Query: 241 GNWSEADIVAFLKTGRNSHSAAFGGMADVVANSTQYMTEEDLSAMAKYLKSLKPVKDGTP 300 GNWSEADIVAFLKTGRNSHSAAFGGMADVVANSTQYMTEEDLSAMAKYLKSLKPVKDGTP Sbjct: 241 GNWSEADIVAFLKTGRNSHSAAFGGMADVVANSTQYMTEEDLSAMAKYLKSLKPVKDGTP 300 Query: 301 ALAYDDKTHQALRKGSDQSPGAMAFLNNCAACHRSSGKGYDETFPSLALSPTVNAENPAS 360 ALAYDDKTHQALRKGSDQSPGAMAFLNNCAACHRSSGKGYDETFPSLALSPTVNAENPAS Sbjct: 301 ALAYDDKTHQALRKGSDQSPGAMAFLNNCAACHRSSGKGYDETFPSLALSPTVNAENPAS 360 Query: 361 LIRIVLEGAEMPWTHKAPTQFAMPAFGSRLSDQEVAEVVTFIRSSWGNQASSVSASDVAK 420 LIRIVLEGAEMPWTHKAPTQFAMPAFGSRLSDQEVAEVVTFIRSSWGNQASSVSASDVAK Sbjct: 361 LIRIVLEGAEMPWTHKAPTQFAMPAFGSRLSDQEVAEVVTFIRSSWGNQASSVSASDVAK 420 Query: 421 VRKQLPEKKTVEAGSAPVAKQ 441 VRKQLPEKKTVEAGSAPVAKQ Sbjct: 421 VRKQLPEKKTVEAGSAPVAKQ 441 >gi|300313762|ref|YP_003777854.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 438 bits (1127), Expect = e-124, Method: Compositional matrix adjust. Identities = 212/397 (53%), Positives = 278/397 (70%), Gaps = 5/397 (1%) Query: 31 SADQQLVQRGEYLAKAGDCVACHTAKGGKPFAGGLAIATPIGTVYSSNITPDKENGIGNY 90 SA+ Q + RG YLA+AGDCVACHT+KGG PFAGGLA+A+PIG +YS+NITPDK++GIG++ Sbjct: 77 SAEDQQILRGAYLARAGDCVACHTSKGGAPFAGGLALASPIGAIYSTNITPDKQHGIGDW 136 Query: 91 SEEDFDRALRHGIRKDGASLYPAMPYPSYAKVKPADVKALYAYFMHGVQADPAPNRGVDI 150 S EDF R +R G+ K G ++YPAMPYPSY+++ D++ALYAYF V NR DI Sbjct: 137 SYEDFARLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQALYAYFSKAVPPSAQENRANDI 196 Query: 151 TWPLSMRWPLSIWRKVFAPAVAVDGPEDNS--PLVRGQYLVEGLGHCGACHTPRGVGMQE 208 WPLSMRWPL++WRKVFAP A P S L RG YLVEGLGHCG+CH+PR V MQE Sbjct: 197 PWPLSMRWPLALWRKVFAPTPAPYTPAAGSDQELARGAYLVEGLGHCGSCHSPRAVTMQE 256 Query: 209 KALSNDSSQ-FLSGG-VIDGYLANNLRGDARDGLGNWSEADIVAFLKTGRNSHSAAFGGM 266 KAL D + FLSGG V+DG+ +LR + G+ WS+AD+V FL+TGRN ++A+FG M Sbjct: 257 KALKEDGGRLFLSGGQVVDGWSVPSLRNEHGGGIAGWSQADLVEFLRTGRNQYTASFGAM 316 Query: 267 ADVVANSTQYMTEEDLSAMAKYLKSLKPVKDGTPALAYDDKTHQALRKGSDQSPGAMAFL 326 DV+ +S QYM++ DL+AMA+YL SL P + P YD T QA G PGA +L Sbjct: 317 NDVIEDSMQYMSDADLNAMARYLLSLPPRQQAAP-YRYDSATAQAAYDGRPDGPGARIYL 375 Query: 327 NNCAACHRSSGKGYDETFPSLALSPTVNAENPASLIRIVLEGAEMPWTHKAPTQFAMPAF 386 + CAACHRS+G GY + FP+LA +P + + S IRI+L+G P T A M + Sbjct: 376 DRCAACHRSNGTGYGKAFPALAGNPVLQTSDATSAIRIILQGGRQPSTASATAGLVMAPY 435 Query: 387 GSRLSDQEVAEVVTFIRSSWGNQASSVSASDVAKVRK 423 L DQ+VAEV ++I+++WGN+ + +A++VAKVRK Sbjct: 436 AQLLDDQQVAEVTSYIQTAWGNRGGTTTAAEVAKVRK 472 >gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-dehydrogenase cytochrome c subunit [Herbaspirillum seropedicae SmR1] Length = 423 Score = 385 bits (990), Expect = e-108, Method: Compositional matrix adjust. Identities = 196/406 (48%), Positives = 259/406 (63%), Gaps = 13/406 (3%) Query: 29 APSADQQLVQRGEYLAKAGDCVACHTAKGGKPFAGGLAIATPIGTVYSSNITPDKENGIG 88 A +ADQ LV RG+YLA+AGDC+ACH+A G ++GGLAI + G +YS+NITPDKE+GIG Sbjct: 24 AQAADQDLVARGQYLARAGDCMACHSAAGKPAYSGGLAIDSGHGIIYSTNITPDKEHGIG 83 Query: 89 NYSEEDFDRALRHGIRKDGASLYPAMPYPSYAKVKPADVKALYAYFMHGVQADPAPNRGV 148 NYSE F A+RHG+R DG LYPAMPYPSYAKV D+ ALY YFM GVQ + Sbjct: 84 NYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDEDIHALYTYFMQGVQPVASTPPAS 143 Query: 149 DITWPLSMRWPLSIWRKVFA---PAVAVDGPEDNSPLVRGQYLVEGLGHCGACHTPRGVG 205 +++P ++RW + +W FA P DG ++ + RG YLVEGLGHCG+CHTPRGV Sbjct: 144 SMSFPFNIRWGMKLWNVFFANDKPFREQDGW--SAEIKRGAYLVEGLGHCGSCHTPRGVA 201 Query: 206 MQEKALSNDSSQFLSGGVIDGYLANNLRGDARDGLGNWSEADIVAFLKTGRNSHSAAFGG 265 M EKA + +QFLSGG ++G+ +LR G+ +WS DIV +L+TGRN ++ G Sbjct: 202 MNEKASDSSQAQFLSGGDLNGWAVPSLR-----GMPHWSAQDIVDYLQTGRNKTASVAGE 256 Query: 266 MADVVANSTQYMTEEDLSAMAKYLKSLKPVKDGTPALAYD---DKTHQALRKGSDQSPGA 322 M+ VV +ST ++ EDL AMA YLK+L PV D T L D + G Sbjct: 257 MSLVVEHSTSHLKREDLQAMAAYLKTLSPVASSGSGRVIPQGVDATVSKLTAAKDLTLGE 316 Query: 323 MAFLNNCAACHRSSGKGYDETFPSLALSPTVNAENPASLIRIVLEGAEMPWTHKAPTQFA 382 +L+NCAACH +G+G FP L + VNAENP++L+ ++L GA P T KAP+ Sbjct: 317 RLYLDNCAACHFVTGRGAPGIFPVLDGATVVNAENPSALLHVILAGARTPSTEKAPSILV 376 Query: 383 MPAFGSRLSDQEVAEVVTFIRSSWGNQASSVSASDVAKVRKQLPEK 428 MP F RLSD+E A + TF+R WGN A VS +V K+R + ++ Sbjct: 377 MPGFAHRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLRAGMDKQ 422 >gi|300310719|ref|YP_003774811.1| cytochrome c protein [Herbaspirillum seropedicae SmR1] Length = 431 Score = 298 bits (764), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 176/402 (43%), Positives = 236/402 (58%), Gaps = 24/402 (5%) Query: 29 APSADQQLVQRGEYLAKAGDCVACHTAKGGKPFAGGLAIATPIGTVYSSNITPDKENGIG 88 AP +QL Q G+YLA+AG+C+ACHTA+GG P+AGG AIA+P GT+Y+SNITPDK GIG Sbjct: 41 APDPTRQLAQ-GQYLARAGNCMACHTARGGAPYAGGRAIASPFGTLYTSNITPDKTTGIG 99 Query: 89 NYSEEDFDRALRHGIRKDGASLYPAMPYPSYAKVKPADVKALYAYFMHGVQADPAPNRGV 148 +S +DF RAL HG K+G LYPA PY SY ++ AD AL+AY + + N+ Sbjct: 100 QWSADDFWRALHHGRGKEGQFLYPAFPYTSYTRLSRADSDALFAY-LQSLPPVARANQAH 158 Query: 149 DITWPLSMRWPLSIWRKV-FAPAVAVDGPEDNSPLVRGQYLVEGLGHCGACHTPRG-VGM 206 + +P R L +WR + F P P ++ RG YLV+G GHC ACH+ R +G Sbjct: 159 TLRFPYDQRMLLGLWRALYFTPQSYQPDPRQDAQWNRGAYLVQGAGHCSACHSARNRLGA 218 Query: 207 QEKALSNDSSQFLSGGVIDG--YLANNLRGDARDGLGNWSEADIVAFLKTGRNSHSAAFG 264 + L+ L+GGVI + A L G A DGL +WS ADI A LKTG + H+ G Sbjct: 219 SIEPLA------LAGGVIPALQWYAPPLHGGA-DGLQDWSTADIAALLKTGVSPHAVTLG 271 Query: 265 GMADVVANSTQYMTEEDLSAMAKYLKSLKPVKDGTPALAYDDKTHQALRKGSD----QSP 320 M+++V S QY+ + D+SAMA YLKSL PA A DD A + ++ Sbjct: 272 PMSEIVGRSLQYLNDADVSAMAVYLKSL-------PASAADDAARPASVEPAEAERIMKQ 324 Query: 321 GAMAFLNNCAACHRSSGKGYDETFPSLALSPTVNAENPASLIRIVLEGAEMPWTHKAPTQ 380 G + C CH G+G +P LA + + +PA+ +R VL G P T P Sbjct: 325 GKQLYGTLCVDCHGGKGQGSAPDYPPLAGNRAIAGPHPANAVRAVLNGGFPPSTAGNPYP 384 Query: 381 FAMPAFGSRLSDQEVAEVVTFIRSSWGNQASSVSASDVAKVR 422 F MP FG +LSDQEVA VV+++R+SWGNQ VSA++V + R Sbjct: 385 FGMPPFGPQLSDQEVAAVVSYVRNSWGNQGGLVSAAEVNRYR 426 >gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 499 Score = 282 bits (721), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 168/414 (40%), Positives = 239/414 (57%), Gaps = 29/414 (7%) Query: 36 LVQRGEYLAKAGDCVACHTAKGGKPFAGGLAIATPIGTVYSSNITPDKENGIGNYSEEDF 95 L++RG YLA A DC+ACHTA GKP AGGLAIA+P+G + ++NITP K +GIGNY+EE F Sbjct: 52 LIERGHYLAIASDCIACHTAPRGKPMAGGLAIASPVGEIIATNITPSKTHGIGNYTEEQF 111 Query: 96 DRALRHGIRKDGASLYPAMPYPSYAKVKPADVKALYAYFMHGVQADPAPNRGVDITWPLS 155 ALR G+R DGA+LYPAMPY +YA + ADV+ALYAYFM GV R D+ +P++ Sbjct: 112 AAALRRGVRADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPVDVATRPTDLPFPMN 171 Query: 156 MRWPLSIWRKVFAPAVAV-DGPEDNSPLVRGQYLVEGLGHCGACHTPRGVGMQEKALSND 214 +RW + W +F + P+ ++ +RG+YL EG HC CHTPRG MQEK Sbjct: 172 LRWSMKAWNALFLKEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCHTPRGWLMQEK----- 226 Query: 215 SSQFLSGGVIDGYLANNLRGDARDGLGNWSEADIVAFLKTGR-NSHSAAFGGMADVVANS 273 L+G + + A N+ G+G+WS+ D+VA+L+TGR + A G MA+ + +S Sbjct: 227 PDLQLAGAQVGPWYAPNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAAGSMAEAITHS 286 Query: 274 TQYMTEEDLSAMAKYLKSLKPVKDGTPALA---YDD-KTHQAL-----RKGSDQSP---- 320 +TEEDL A+A Y+K + G + +D K AL R S +SP Sbjct: 287 FSRLTEEDLRAIAVYIKDRPAIATGDAQGSGSRFDQGKAGNALAQFRGRTLSAESPEEIR 346 Query: 321 GAMAFLNNCAACHRSSGKG-YDETFPSLALSPTVNAENPASLIRIVLEGAEMPWTHKAPT 379 GA F +CA+CH +G+G D +PSL + N +LI +L G + T + Sbjct: 347 GARIFSASCASCHGYNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGVDRE-TEQGGH 405 Query: 380 QFAMPAFG------SRLSDQEVAEVVTFIRSSWGNQASSVSASDVAKVRKQLPE 427 F MP FG ++LS+ +VA + ++ +G A++V A DV +R+ P Sbjct: 406 VF-MPPFGDQPNALNKLSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQGGPR 458 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,397,608 Number of extensions: 60492 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 8 Length of query: 441 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 353 Effective length of database: 1,203,190 Effective search space: 424726070 Effective search space used: 424726070 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)