BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0011.p (596 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312639|ref|YP_003776731.1| choline dehydrogenase [Herbaspi... 1217 0.0 gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbas... 632 0.0 gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like prot... 569 e-163 >gi|300312639|ref|YP_003776731.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 1217 bits (3148), Expect = 0.0, Method: Compositional matrix adjust. Identities = 588/596 (98%), Positives = 590/596 (98%), Gaps = 3/596 (0%) Query: 1 MAIKKDKVDVVMVGLGWTGSIMGMELTEAGLSVLALERGDMRDTNTDTPYPKAVDELKYS 60 MAIKKDKVDVVMVGLGWTGSIMGMELTEAGLSVLALERGDMRDTNTDTPYPKAVDELKYS Sbjct: 1 MAIKKDKVDVVMVGLGWTGSIMGMELTEAGLSVLALERGDMRDTNTDTPYPKAVDELKYS 60 Query: 61 VRGALFQDLSRETVTIRHTLSDVAVPYRQHGSFLLGDGVGGAGFHWNGMMYRNLPEELEM 120 VRGALFQDLSRETVTIRHTLSDVAVPYRQHGSFLLGDGVGGAGFHWNGMMYRNLPEELEM Sbjct: 61 VRGALFQDLSRETVTIRHTLSDVAVPYRQHGSFLLGDGVGGAGFHWNGMMYRNLPEELEM 120 Query: 121 RTHHEQKYGKKFIPEDMTIQDHGVTYKELEPFYDFSEKVMAVSGKAGNLKGQIVPGGNPL 180 RTHHEQKYGKKFIPEDMTIQDHGVTYKELEPFYDFSEKVMAVSGKAGNLKGQIVPGGNPL Sbjct: 121 RTHHEQKYGKKFIPEDMTIQDHGVTYKELEPFYDFSEKVMAVSGKAGNLKGQIVPGGNPL 180 Query: 181 EGERSDEFPTPPLQYQYSASLVEKAMRDVGYHPYPAPAANASQPFTNPYGVRIGPCNYCG 240 EGERSDEFPTPPLQYQYSASLVEKAMRDVGYHPYPAPAANASQPFTNPYGVRIGPCNYCG Sbjct: 181 EGERSDEFPTPPLQYQYSASLVEKAMRDVGYHPYPAPAANASQPFTNPYGVRIGPCNYCG 240 Query: 241 FCENYGCYMYSKASPQTTILPVLLKRKNFEVRTKAQVIKVNLDASGKRATGVTYIDAQGR 300 FCENYGCYMYSKASPQTTILPVLLKRKNFEVRTKAQVIKVNLDASGKRATGVTYIDAQGR Sbjct: 241 FCENYGCYMYSKASPQTTILPVLLKRKNFEVRTKAQVIKVNLDASGKRATGVTYIDAQGR 300 Query: 301 EVEQPADLVLLTAFQTHNVRLLLLSGIGKPYDPVTGEGVVGKNFAYQYNGGINVIMPKGT 360 EVEQPADLVLLTAFQTHNVRLLLLSGIGKPYDPVTGEGVVGKNFAYQYNGGINVIMPKGT Sbjct: 301 EVEQPADLVLLTAFQTHNVRLLLLSGIGKPYDPVTGEGVVGKNFAYQYNGGINVIMPKGT 360 Query: 361 QFNPFIGTG*KPDFFLGMDDLNADQFDHGPLGFIGGASIRHVRYGGRPIKQAGTMPGEKV 420 QFNPFIGTG +GMDDLNADQFDHGPLGFIGGASIRHVRYGGRPIKQAGTMPGEKV Sbjct: 361 QFNPFIGTG---AGGVGMDDLNADQFDHGPLGFIGGASIRHVRYGGRPIKQAGTMPGEKV 417 Query: 421 PSWGSEWKASVQDAYQRTLTIGMSGSVMAYRDCYLSLDPTYKDANGQPLLRLTLNWKENE 480 PSWGSEWKASVQDAYQRTLTIGMSGSVMAYRDCYLSLDPTY+DANGQPLLRLTLNWKENE Sbjct: 418 PSWGSEWKASVQDAYQRTLTIGMSGSVMAYRDCYLSLDPTYRDANGQPLLRLTLNWKENE 477 Query: 481 GKMLTYIASQMEKVGQAMNPEKVVKAVRKTGSSWDTRVYQSTHLTGGAIMGTTPKNSVVN 540 GKMLTYIASQMEKVGQAMNPEKVVKAVRKTGSSWDTRVYQSTHLTGGAIMGTTPKNSVVN Sbjct: 478 GKMLTYIASQMEKVGQAMNPEKVVKAVRKTGSSWDTRVYQSTHLTGGAIMGTTPKNSVVN 537 Query: 541 KYLQSWDVPNVFVYGACAFPQNMGYNPTGLVTALAYHSAKAIREQYLKNPGPLVQA 596 KYLQSWDVPNVFVYGACAFPQNMGYNPTGLVTALAYHSAKAIREQYLKNPGPLVQA Sbjct: 538 KYLQSWDVPNVFVYGACAFPQNMGYNPTGLVTALAYHSAKAIREQYLKNPGPLVQA 593 >gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 632 bits (1631), Expect = 0.0, Method: Compositional matrix adjust. Identities = 305/598 (51%), Positives = 394/598 (65%), Gaps = 7/598 (1%) Query: 1 MAIKKDK-VDVVMVGLGWTGSIMGMELTEAGLSVLALERGDMRDTNTDTPYPKAVDELKY 59 MA K K VDVV+VG GWTG+IM E+TEAG V+ALERG RDT D YPK ++EL+Y Sbjct: 1 MANKNMKPVDVVIVGFGWTGAIMAKEMTEAGQRVVALERGVYRDTYPDGAYPKTINELEY 60 Query: 60 SVRGALFQDLSRETVTIRHTLSDVAVPYRQHGSFLLGDGVGGAGFHWNGMMYRNLPEELE 119 R LFQ+L++ + T R D A+PYRQ F G+GVGGAG HW+G +R LPEEL Sbjct: 61 QQRFKLFQNLNKSSFTFRRKTGDSAIPYRQIAMFKPGEGVGGAGLHWSGCHWRILPEELR 120 Query: 120 MRTHHEQKYGKKFIPEDMTIQDHGVTYKELEPFYDFSEKVMAVSGKAGNLKGQIV-PGGN 178 MR+H+E++YGK FIP+DMT+QD GV+Y+ELEP++DF+EK+M SG A + G++V GN Sbjct: 121 MRSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYRVGGKVVDESGN 180 Query: 179 PLEGERSDEFPTPPLQYQYSASLVEKAMRDVGYHPYPAPAANASQPFTNPYGVRIGPCNY 238 P E +RSD FP P + QY A+L KA + G HP+ P+ANA+ P+ N YG ++GPC + Sbjct: 181 PFEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYVNQYGCQMGPCTF 240 Query: 239 CGFCENYGCYMYSKASPQTTILPVLLKRKNFEVRTKAQVIKVNLDASGKRATGVTYIDAQ 298 CGFC Y CY YSKASP I+P L + FE+R+ V+++ LD+S K+ATGVTY+D Sbjct: 241 CGFCSGYACYNYSKASPNVNIMPALRQSNLFELRSSCNVLRIELDSSRKKATGVTYVDIN 300 Query: 299 GREVEQPADLVLLTAFQTHNVRLLLLSGIGKPYDPVTGEGVVGKNFAYQYNGGINVIMPK 358 G V QPA +V+ + F +N RL LLSGIGKPYDPV+ G VG+N A+Q IN Sbjct: 301 GDTVFQPASIVIASTFAYNNARLFLLSGIGKPYDPVSNTGAVGRNIAFQMMSTINAFFEP 360 Query: 359 GTQFNPFIGTG*KPDFFLGMDDLNADQFDHGPLGFIGGASIRHVRYGGRPIKQAGTMPGE 418 G N FIG G + +DD N D DHGPLGF+GG+ I G +PI G Sbjct: 361 GKNINGFIGAGGNG---VAVDDFNGDHMDHGPLGFVGGSPIWCNPSGSKPISGIAVPSG- 416 Query: 419 KVPSWGSEWKASVQDAYQRTLTIGMSGSVMAYRDCYLSLDPTYKDANGQPLLRLTLNWKE 478 P WG WK +V+D+Y T++ + G+ M YRD Y+ LDP YKDA GQPLLR T +WK+ Sbjct: 417 -TPRWGRAWKQAVKDSYLNTMSFDVHGANMVYRDVYVDLDPNYKDAFGQPLLRFTFDWKD 475 Query: 479 NEGKMLTYIASQMEKVGQAMNPEKVVKAVRKTGSSWDTRVYQSTHLTGGAIMGTTPKNSV 538 N+ +M Y+ Q K+ + ++ V+ G++ D R YQ+TH GG MGT P SV Sbjct: 476 NDIRMSRYVTDQAVKIAHELKAREIKVTVKDFGAAPDLRSYQTTHWAGGVAMGTDPSTSV 535 Query: 539 VNKYLQSWDVPNVFVYGACAFPQNMGYNPTGLVTALAYHSAKAIREQYLKNPGPLVQA 596 VN+YLQSWDV NVF G+ F Q +GYNPTG AL Y SA+AIREQYL +P PLVQA Sbjct: 536 VNRYLQSWDVHNVFAVGSGVFAQGIGYNPTGAAVALTYWSARAIREQYLNDPRPLVQA 593 >gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like protein [Herbaspirillum seropedicae SmR1] Length = 590 Score = 569 bits (1467), Expect = e-163, Method: Compositional matrix adjust. Identities = 285/595 (47%), Positives = 380/595 (63%), Gaps = 9/595 (1%) Query: 3 IKKDKVDVVMVGLGWTGSIMGMELTEAGLSVLALERGDMRDTNTDTPYPKAVDELKYSVR 62 I D VDVV+VGLGW GS+M +EL +AGL V ALERG R D YPK D+ Y+ R Sbjct: 4 ITNDDVDVVIVGLGWAGSLMAIELAQAGLKVRALERGPDRPPE-DFAYPKPADQYGYATR 62 Query: 63 GALFQDLSRETVTIRHTLSDVAVPYRQHGSFLLGDGVGGAGFHWNGMMYRNLPEELEMRT 122 +F +T+R+ + A+P R+ G+F G GVGG+G HW ++ R P +L+++T Sbjct: 63 NKVFATPRDAALTVRYNTAQQALPTRKWGAFAPGTGVGGSGLHWTAVLIRATPTDLKLKT 122 Query: 123 HHEQKYGKKFIPEDMTIQDHGVTYKELEPFYDFSEKVMAVSGKAGNLKGQIVPGGNPLEG 182 + +Q Y + +M I+D T+ E+EP YDF +KV +SG GNL+GQI PGG+P EG Sbjct: 123 YADQAYKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCGLSGTTGNLRGQIQPGGDPFEG 182 Query: 183 ERSDEFPTPPLQYQYSASLVEKAMRDVGYHPYPAPAANASQPFTNPYGVRIGPCNYCGFC 242 RS+ +P P L Y+ +L R +GYHP+P P+AN SQ +TNPYG +I PCNYCGFC Sbjct: 183 PRSNPYPLPALHDTYNNTLFADVARKLGYHPFPNPSANVSQAWTNPYGAQIAPCNYCGFC 242 Query: 243 ENYGCYMYSKASPQTTILPVLLKRKNFEVRTKAQVIKVNLDASGKRATGVTYIDAQGREV 302 Y C YSKASPQTTIL V+ + NF+ + A VI+V+L A K A GVTY+D +EV Sbjct: 243 SKYPCLNYSKASPQTTILDVVKRLPNFDYKVNANVIRVDLHADKKTARGVTYVDENMKEV 302 Query: 303 EQPADLVLLTAFQTHNVRLLLLSGIGKPYDPVTGEGVVGKNFAYQYNGGINVIMPKGTQF 362 QPA +V+L++FQ NVRL+LLSGIGKPYDP+T GV+G+N+A+ NGG + K F Sbjct: 303 FQPAKIVILSSFQFANVRLMLLSGIGKPYDPITETGVIGRNYAFLSNGGATLFF-KDKHF 361 Query: 363 NPFIGTG*KPDFFLGMDDLNADQFDHG-PLGFIGGASIRHVRYGGRPIKQAGTMPGEKVP 421 N F G + F +D++ FD G LGFIGGA I + G PI GT P Sbjct: 362 NSFATAGATGEMF---NDISPGNFDDGVKLGFIGGAKIHSSQASGAPI---GTALPPGTP 415 Query: 422 SWGSEWKASVQDAYQRTLTIGMSGSVMAYRDCYLSLDPTYKDANGQPLLRLTLNWKENEG 481 SWG WK + D Y ++ I ++ + M+YR +L LDP YKD GQPLLR+T +W++NE Sbjct: 416 SWGRGWKEGMIDWYDHSMKISITTTCMSYRGHFLDLDPNYKDPWGQPLLRMTFDWRQNEL 475 Query: 482 KMLTYIASQMEKVGQAMNPEKVVKAVRKTGSSWDTRVYQSTHLTGGAIMGTTPKNSVVNK 541 K+ Y+ + K+ + +NP+ + ++ S WD Y STH GGAIMG +P++S +NK Sbjct: 476 KLQRYLRDIVLKISKELNPDHMSESFLALDSHWDITKYVSTHNVGGAIMGDSPRDSALNK 535 Query: 542 YLQSWDVPNVFVYGACAFPQNMGYNPTGLVTALAYHSAKAIREQYLKNPGPLVQA 596 YLQSWDV NVFV G AFPQN NPT + A+ +A AI+ QYLKNPGPLVQA Sbjct: 536 YLQSWDVHNVFVPGGNAFPQNFQANPTATIGAITLMAAHAIKTQYLKNPGPLVQA 590 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,045,830 Number of extensions: 93254 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 3 Length of query: 596 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 505 Effective length of database: 1,188,985 Effective search space: 600437425 Effective search space used: 600437425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)