BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0083.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312345|ref|YP_003776437.1| cell wall surface anchor protei... 78 2e-15 >gi|300312345|ref|YP_003776437.1| cell wall surface anchor protein [Herbaspirillum seropedicae SmR1] Length = 7175 Score = 77.8 bits (190), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 88/282 (31%), Positives = 131/282 (46%), Gaps = 53/282 (18%) Query: 1 LQLKVQDAAGNQSTVKTQAYVLDTTAPAITFSNLALSADTGSSNTDFITNTAAQTITATL 60 ++++ D AGN S +T + +D TAPA + AL DTGSS+TD +T+ + ++ Sbjct: 4956 VKVRQTDKAGNDSVEQTLVFTVDMTAPAKLSA--ALVKDTGSSSTDRLTSDGSFSVNGI- 5012 Query: 61 SVAPAGSERVYGSLDNGATWTN-------ITGKVSGTTLSWNGVTLSGSNTLLLRVTDNA 113 AG+ + S DNG +W + + G GT + G ++LR D A Sbjct: 5013 ---EAGASWQW-SEDNGTSWIDGVGVGARVKGAADGTGF------VDGVKNVVLRQVDVA 5062 Query: 114 GNDGTVKS-QSYTLDVTAPAITVSNVALSADTGVSNTDFITATAAQTITATLSSTLAGSD 172 GN G V S+TLD TAP +AL DTG+S +D IT + G D Sbjct: 5063 GNVGPVSDVLSFTLDTTAPGTPA--IALRRDTGISGSDRIT-----------NDGTVGID 5109 Query: 173 RVYG------SLDNGATWTDLTSQVTGTALSWV------GATLQGGTHTLLLKVQDNAGN 220 R+ G S+D G TW D G+A+S GA G +L++ D AGN Sbjct: 5110 RLEGTSSWQYSVDGGTTWID------GSAISVKFPGAADGADGSDGAKNVLMRQVDAAGN 5163 Query: 221 DGA-IATQAYTLEVPAATAPSGNLAGLLSPSRLPVASTDTPI 261 A A ++TL+ A AP +L+ PS+ + D+ + Sbjct: 5164 ASAPTARYSFTLDTTAPVAPGLSLSNDTGPSKTDRVTNDSTL 5205 Score = 71.2 bits (173), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 91/240 (37%), Positives = 129/240 (53%), Gaps = 36/240 (15%) Query: 3 LKVQDAAGNQSTVKTQ-AYVLDTTAPAITFSNLALSADTGSSNTDFITNTAAQTITATLS 61 ++ DAAGN S + ++ LDTTAP L+LS DTG S TD +TN +TL+ Sbjct: 5155 MRQVDAAGNASAPTARYSFTLDTTAP--VAPGLSLSNDTGPSKTDRVTN------DSTLN 5206 Query: 62 VA--PAGSERVYGSLDNGATWTNITGKVSGTTLSWNGVTLSGSNTLLLRVTDNAGNDGTV 119 VA AG+ Y S DNG TW + G SG+++ G+ G T+++R D AGN G Sbjct: 5207 VAGLEAGASWQY-STDNGTTW--LAG--SGSSVRLKGLP-DGVTTVIVRQMDVAGNQGPA 5260 Query: 120 -KSQSYTLDVTAPAITVSNVALSADTGVSNTDFITATAAQTITATLSSTLAGSDRVYGSL 178 +S S+ D TAPA VS V L+ DTG S +D IT T L+ G+ Y SL Sbjct: 5261 SESFSFVQDATAPA-KVSMV-LARDTGASTSDRITNDG----TINLTGLENGATWQY-SL 5313 Query: 179 DNGATWTDLTSQVTGTALSWVGATLQG-----GTHTLLLKVQDNAGNDGAIA-TQAYTLE 232 +NG +WT +GT++ GA + G G T++++ D AGN+ A + + A TL+ Sbjct: 5314 NNGGSWT----AGSGTSVKIKGA-VDGVGNFDGNKTIVVRQTDLAGNNSAFSDSLAITLD 5368 Score = 61.6 bits (148), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 77/234 (32%), Positives = 118/234 (50%), Gaps = 27/234 (11%) Query: 3 LKVQDAAGNQSTV-KTQAYVLDTTAPAITFSNLALSADTGSSNTDFITNTAAQTITATLS 61 ++ D AGNQ ++ ++V D TAPA ++ L+ DTG+S +D ITN T L+ Sbjct: 5248 VRQMDVAGNQGPASESFSFVQDATAPAKV--SMVLARDTGASTSDRITNDG----TINLT 5301 Query: 62 VAPAGSERVYGSLDNGATWTNITGKVSGTTLSWNGV-TLSGSNTLLLRVTDNAGNDGTV- 119 G+ Y SL+NG +WT +G + +GV G+ T+++R TD AGN+ Sbjct: 5302 GLENGATWQY-SLNNGGSWTAGSGTSVKIKGAVDGVGNFDGNKTIVVRQTDLAGNNSAFS 5360 Query: 120 KSQSYTLDVTAPAITVSNVALSADTGVSNTDFITATAAQTITATLSSTLAGSDRVYGSLD 179 S + TLD APA ++AL+ DTGV N D IT+ +T L + S D Sbjct: 5361 DSLAITLDTLAPAKL--SLALARDTGVIN-DKITSDGTVNVTG-----LEANASWQWSTD 5412 Query: 180 NGATWTDLTSQVTGTALSWVGATLQGGTHTLLLKVQDNAGNDGAIA-TQAYTLE 232 G W S+ +GT+ G G ++ + D AGN GA++ ++TL+ Sbjct: 5413 GGVNW----SRGSGTSFLVSG----DGPKAVMARQSDLAGNLGAVSDVLSFTLD 5458 Score = 57.4 bits (137), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 68/186 (36%), Positives = 95/186 (51%), Gaps = 23/186 (12%) Query: 7 DAAGNQSTV-KTQAYVLDTTAPA-ITFSNLALSADTGSSNTDFITNTAAQTITATLSVAP 64 DAAGN S + + ++ D PA IT S L DTG S +D ITN T +S Sbjct: 5729 DAAGNVSPISEPLDFIRDVVPPAKITAS---LMKDTGVSASDRITNDG----TVGVSTME 5781 Query: 65 AGSERVYGSLDNGATWTNITG---KVSGTTLSWNGVTLSGSNTLLLRVTDNAGNDGTVKS 121 +G+ Y SLD G TW N G KV G + G G ++L+R D AGN+G + Sbjct: 5782 SGATLQY-SLDGGVTWLNAVGNNFKVQGA--ADGGSNTDGPKSVLVRQVDVAGNEGQASA 5838 Query: 122 -QSYTLDVTAPAITVSNVALSADTGVSNTDFITATAAQTITATLSSTLAGSDRVYGSLDN 180 S+TLD T P + +AL+ DTG S++D +T+ T +S G+ Y SLD+ Sbjct: 5839 VLSFTLD-TLPQGKLG-LALANDTGTSSSDGVTSDG----TLNISGLETGASWQY-SLDD 5891 Query: 181 GATWTD 186 G+ W + Sbjct: 5892 GSRWVN 5897 Score = 55.8 bits (133), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 71/235 (30%), Positives = 115/235 (48%), Gaps = 28/235 (11%) Query: 10 GNQSTVKTQAYVLDTTAPAITFSNLALSADTGSSNTDFITNTAAQTITATLSVAPAGSER 69 G S T LDTT P + + + L D+G S +D +TN + + + V S R Sbjct: 4875 GIDSIKVTHTVTLDTTIP-VFGAPVRLELDSGQSASDGVTNNG-KVLVNGVEVGANWSYR 4932 Query: 70 VYGSLDNGATWTNITGKVSGTTLSWNGVTLSGSNTLLLRVTDNAGNDGTVKSQSYTLDVT 129 + D+G G S+N V G++T+ +R TD AGND ++ +T+D+T Sbjct: 4933 I----DDGPYQPGGRG-------SFN-VATDGAHTVKVRQTDKAGNDSVEQTLVFTVDMT 4980 Query: 130 APAITVSNVALSADTGVSNTDFITATAAQTITATLSSTLAGSDRVYGSLDNGATWTD--- 186 APA + AL DTG S+TD +T+ + ++ + AG+ + S DNG +W D Sbjct: 4981 APAKL--SAALVKDTGSSSTDRLTSDGSFSV----NGIEAGASWQW-SEDNGTSWIDGVG 5033 Query: 187 LTSQVTGTALSWVGATLQGGTHTLLLKVQDNAGNDGAIA-TQAYTLEVPAATAPS 240 + ++V G A G G ++L+ D AGN G ++ ++TL+ A P+ Sbjct: 5034 VGARVKGAA---DGTGFVDGVKNVVLRQVDVAGNVGPVSDVLSFTLDTTAPGTPA 5085 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.310 0.125 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,346,475 Number of extensions: 53868 Number of successful extensions: 258 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 241 Number of HSP's successfully gapped: 12 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)