BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0070.p (478 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 912 0.0 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 149 4e-37 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 143 3e-35 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 133 3e-32 gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 128 8e-31 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 912 bits (2358), Expect = 0.0, Method: Compositional matrix adjust. Identities = 474/478 (99%), Positives = 474/478 (99%) Query: 1 MSCPSTPAAAALAAHPPAAGATLTPRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSL 60 MSCPSTPAAAALAAHPPAAGATLTPRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSL Sbjct: 1 MSCPSTPAAAALAAHPPAAGATLTPRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSL 60 Query: 61 DAPLSTLVWIVDGYTLTFAAMLLVGGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPF 120 DAPLSTLVWIVDGYTLTFAAMLLVGGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPF Sbjct: 61 DAPLSTLVWIVDGYTLTFAAMLLVGGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPF 120 Query: 121 LIAARLLQGLGAAFFMPSSLSLLTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIH 180 LI ARLLQGLGAAFFMPSSLSLLTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIH Sbjct: 121 LIVARLLQGLGAAFFMPSSLSLLTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIH 180 Query: 181 WLGWRSVFLINVPVGLVGLVMARKLIPQVAGHARALNLSSHLAGVVMLAGLSFVLIQGPV 240 WLGWRSVFLINVPVGLVGLVMARKLIPQV GHARALNLSSHLAGVVMLAGLSFVLIQGPV Sbjct: 181 WLGWRSVFLINVPVGLVGLVMARKLIPQVGGHARALNLSSHLAGVVMLAGLSFVLIQGPV 240 Query: 241 YGWTSPRILAGLAVVLLAGAALIRHELRGIAPLLPKALFATPQFAAANGIGFLINFGVYG 300 YGWTSPRILAGLAV L AGAALIRHELRGIAPLLPKALFATPQFAAANGIGFLINFGVYG Sbjct: 241 YGWTSPRILAGLAVALSAGAALIRHELRGIAPLLPKALFATPQFAAANGIGFLINFGVYG 300 Query: 301 NLFYLALFLQQGRGADALQTGLQLVPMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTV 360 NLFYLALFLQQGRGADALQTGLQLVPMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTV Sbjct: 301 NLFYLALFLQQGRGADALQTGLQLVPMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTV 360 Query: 361 FTALAMGFSPATPYWLLALVCASANLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQ 420 FTALAMGFSPATPYWLLALVCASANLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQ Sbjct: 361 FTALAMGFSPATPYWLLALVCASANLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQ 420 Query: 421 IGALVGVALMGAILHSVTGWNLRNPLAFGVMAAGYASALWLVWRFVGVVKEQWQVRRA 478 IGALVGVALMGAILHSVTGWNLRNPLAFGVMAAGYASALWLVWRFVGVVKEQWQVRRA Sbjct: 421 IGALVGVALMGAILHSVTGWNLRNPLAFGVMAAGYASALWLVWRFVGVVKEQWQVRRA 478 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 149 bits (377), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 114/313 (36%), Positives = 159/313 (50%), Gaps = 2/313 (0%) Query: 15 HPPAAGATLTPRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGY 74 +P A L+ AG + L + G M +LD VV + L +A L A S + W+V Y Sbjct: 22 YPAAMSLRLSTSAGRILLTG-SFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSY 80 Query: 75 TLTFAAMLLVGGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIAARLLQGLGAAF 134 L FAA+LL GA ADR+G RR+ L G+ LF L SL C AP+ L AR LQG+GAAF Sbjct: 81 VLCFAALLLPAGATADRYGRRRVLLGGIALFALTSLACALAPSAQALYGARALQGVGAAF 140 Query: 135 FMPSSLSLLTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPV 194 + +L+++ H + D+ R R +W +GL + PL+GGV+ LGWR F +N+P Sbjct: 141 LLAPALAVIGHAFHDEDQRERAWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPA 200 Query: 195 GLVGLVMARKLIPQVAGH-ARALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLA 253 L+ V + +P+ R L+ L+ + L++ LI GP +GWTS +A Sbjct: 201 CLLLAVAVWRHVPESRNPLPRPLDPLGILSSSSAVFLLTWALITGPEHGWTSVACVARGL 260 Query: 254 VVLLAGAALIRHELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGR 313 L I + R P+L +LF F AA F L LFLQ R Sbjct: 261 GGALLLVLFIAWQRRAAHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNAR 320 Query: 314 GADALQTGLQLVP 326 G A QTGL ++P Sbjct: 321 GLSAWQTGLAMLP 333 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 143 bits (360), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 128/456 (28%), Positives = 205/456 (44%), Gaps = 19/456 (4%) Query: 13 AAHPPAAGATLTPRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVD 72 +AH ++G L R +L + + ++ LD T+V L I L W+ Sbjct: 7 SAH--SSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKG-FELYAWVAT 63 Query: 73 GYTLTFAAMLLVGGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIAARLLQGLGA 132 Y LT + + G L D FG + + + +FV AS LCG A + FL+ AR LQG+G Sbjct: 64 AYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQGIGG 123 Query: 133 AFFMPSSLSLLTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINV 192 + + + + ++ D RVR R + S + G+A AVGP +GG L LGWR VF N+ Sbjct: 124 GMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFYCNL 183 Query: 193 PVGLVGLVMARKLIPQVAGHARALNLSSHLAGVVMLAGLSFVLIQG----PVYGWTSPRI 248 P+G++ L + +P + A + G ++++ L P G TS + Sbjct: 184 PIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTSG-M 242 Query: 249 LAGLAVVLLAGAALIRHELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALF 308 L LAV + A AL + E R P+LP + A+ + L F ++ L Y L Sbjct: 243 LVLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAPLL 302 Query: 309 LQQGRGADALQTGLQLVPMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTVFTALAMGF 368 Q G G + GL + P++A I ++++GR+ P L++ L + A +G Sbjct: 303 FQGGFGLSPREAGLLITPLVACITVASIVNGRIITRI-PNPNLMMNLGFALI-AASCLGI 360 Query: 369 ---SPATPYWLLALVCASANLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALV 425 T L V + G+ +P +T Q A + H A A L + R IG ++ Sbjct: 361 VLCDRVTGKGFLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML 420 Query: 426 GVALMGAILHSVTGWNLRNPLAFGVMAAGYASALWL 461 G A+ G ++ + G + L G +A WL Sbjct: 421 GTAITGTLVSQMYGAGVEKAL------EGDHAAQWL 450 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 133 bits (334), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 107/331 (32%), Positives = 163/331 (49%), Gaps = 12/331 (3%) Query: 1 MSCPSTPAAAALAAHPPAAGATLTPRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSL 60 M+ TP+ A PP GA L +L +++++ M +LD ++ NV + I+G+L Sbjct: 1 MATSPTPSQA-YTPPPPLTGANL-----VLGTLSVSLAVFMNVLDSSIANVAIPTISGNL 54 Query: 61 DAPLSTLVWIVDGYTLTFAAMLLVGGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPF 120 + W++ + A + + G L R GA RL++T + LFVLAS LCG AP P Sbjct: 55 GVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPI 114 Query: 121 LIAARLLQGLGAAFFMPSSLSLLTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIH 180 L+AAR+LQGL A +P S SLL Y + + L +W T +A GP +GG + Sbjct: 115 LLAARVLQGLVAGPMVPLSQSLLLGAYPKSK-SSFALALWGMTAVVAPVAGPALGGWITD 173 Query: 181 WLGWRSVFLINVPVGLV--GLVMARKLIPQVAGHARALNLSSHLAGVVMLAGLSFVLIQG 238 W +F IN+PVGL+ G+ +A + H ++ V +A +L +G Sbjct: 174 SYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKG 233 Query: 239 PVYGW-TSPRILAGLAVVLLAGAALIRHELRGIAPLLPKALFATPQFAAANGIGFLINFG 297 W SP I+ + L+A A + EL P++ LF FA I I +G Sbjct: 234 KDLDWFASPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGT-IAISIGYG 292 Query: 298 V-YGNLFYLALFLQQGRGADALQTGLQLVPM 327 V +GNL L +LQQ G ++ +GL P+ Sbjct: 293 VFFGNLVILPQWLQQYLGYRSIDSGLSTAPI 323 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 128 bits (322), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 111/345 (32%), Positives = 162/345 (46%), Gaps = 4/345 (1%) Query: 2 SCPSTPAAAALAAHPPAAGATLTPRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSLD 61 S P+ P AA + H AG L P ++AL I +A LD + N L +A L Sbjct: 3 SQPTAPGTAAPSPHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALDLQ 62 Query: 62 APLSTLVWIVDGYTLTFAAMLLVGGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPFL 121 + VWIV Y L LL AL + G RR+Y+ GL LF ASLLC A + P L Sbjct: 63 TTPAASVWIVTAYQLAMMVALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLPTL 122 Query: 122 IAARLLQGLGAAFFMPSSLSLLTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHW 181 + AR LQGLG A M + +L+ +Y R R +G+ + V +A VGP V ++ Sbjct: 123 VMARFLQGLGGAGIMSVNTALIRFIY-PTRSLGRGVGLNTLIVAIAFTVGPSVASGILAI 181 Query: 182 LGWRSVFLINVPVGLVGLVMARKLIPQVAGHARALNLSSHLAGVVMLAGLSFVLIQGPVY 241 W +F +NVP+G++ +V+A +P + A +L S L L + +G Sbjct: 182 APWPWLFAVNVPLGVLAVVLAVPSLPHTSLARHAFDLRSALLNAAAFGLLILAIGEGAHQ 241 Query: 242 GWTSPRILAGLAVVLLAGAALIRHELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGN 301 S L LA +L G L++ EL AP+LP LF P F + + + + +F G Sbjct: 242 AQASVVGLE-LAAAILCGLFLLKRELSHPAPMLPVDLFRHPMF-SLSAVTAVCSFAAQGL 299 Query: 302 LFY-LALFLQQGRGADALQTGLQLVPMMAVIFFGNLMSGRMSAHW 345 F L F G ++ GL + P + ++GRMS + Sbjct: 300 AFVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDRY 344 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,379,443 Number of extensions: 55943 Number of successful extensions: 461 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 434 Number of HSP's successfully gapped: 24 Length of query: 478 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 389 Effective length of database: 1,198,455 Effective search space: 466198995 Effective search space used: 466198995 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)