BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0051.p (331 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 667 0.0 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 123 2e-29 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 114 1e-26 gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 112 5e-26 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 111 7e-26 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 667 bits (1722), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/331 (100%), Positives = 331/331 (100%) Query: 1 LLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWL 60 LLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWL Sbjct: 127 LLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWL 186 Query: 61 TKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQHKRAFLN 120 TKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQHKRAFLN Sbjct: 187 TKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQHKRAFLN 246 Query: 121 VVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAISDKIG 180 VVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAISDKIG Sbjct: 247 VVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAISDKIG 306 Query: 181 RRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKAEMFPP 240 RRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKAEMFPP Sbjct: 307 RRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKAEMFPP 366 Query: 241 EVRALGVGLSYAVGNAIFGGSAEFVALSLKSAGIESAFYWYVSALCLVALIISLRMPDPQ 300 EVRALGVGLSYAVGNAIFGGSAEFVALSLKSAGIESAFYWYVSALCLVALIISLRMPDPQ Sbjct: 367 EVRALGVGLSYAVGNAIFGGSAEFVALSLKSAGIESAFYWYVSALCLVALIISLRMPDPQ 426 Query: 301 RDGHLKGDVAMNPDEHQGEADAAAGKLHKPA 331 RDGHLKGDVAMNPDEHQGEADAAAGKLHKPA Sbjct: 427 RDGHLKGDVAMNPDEHQGEADAAAGKLHKPA 457 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 123 bits (309), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 85/271 (31%), Positives = 139/271 (51%), Gaps = 15/271 (5%) Query: 2 LARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWLT 61 L ++ QG S GGE+G+S ++ E AP+ RRGFF+S+Q + LLA L + LT Sbjct: 126 LGKMIQGFSAGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAVLNNMLT 184 Query: 62 KAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQHKRAFLNV 121 +L +WGWRVPF+ G + A +Y+R L E +K L+ H++ L + Sbjct: 185 PEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKMRLLI 244 Query: 122 VGFTAGGSLMFYTFTTYMQKYL----VNTAGMDPKVANGVMTGALFVYMILQPIFGAISD 177 GS++ T + Y+ Y+ V G+ + M+ A + I P+ GA+SD Sbjct: 245 ----GAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSD 300 Query: 178 KIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMAL---AILALTIVSFYTSISGLIK 234 KIGR M+ + +V T+P+ FL + SPG L ++ + + ++ ++ L+ Sbjct: 301 KIGRTPFMITSSALYIVLTYPMFVFLTN--SPGFLQLLLLQTVIGVLMTMYFAAMPALL- 357 Query: 235 AEMFPPEVRALGVGLSYAVGNAIFGGSAEFV 265 A++FP R G+ L+Y + IFGG A + Sbjct: 358 ADIFPVATRGTGMSLAYNIAVTIFGGFAGLI 388 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 114 bits (285), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 92/294 (31%), Positives = 148/294 (50%), Gaps = 19/294 (6%) Query: 1 LLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWL 60 ++AR+ QG + GGEY +S ++ E AP +RG + S+Q V GQ LAV GM + Sbjct: 115 VVARMLQGFATGGEYSSSTAFLVESAPAHKRGLYGSWQLV----GQCLAVFCGAGMGALV 170 Query: 61 TK----AELMAWGWRVPFVLGAVGALVAMYLRSSLAETSS-AGARKKKDAGTLKGLLQHK 115 T+ L +WGWR+PFVLG + V +++R + ET + K +A +K L K Sbjct: 171 TRNLSPEALDSWGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVK 230 Query: 116 ---RAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIF 172 R L +G G++ FY M + G+ A V A+ + + P Sbjct: 231 TNLRGVLVSMGQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFA 290 Query: 173 GAISDKIGRRNSML--CFAFFGMVGTFPILHFLKDVSS-PGVAMALAILALTIVSFYTSI 229 GA+SD++GRR +L AF MV +P+ ++ S P + + +L + I + + + Sbjct: 291 GALSDRLGRRTVLLYSSLAFLLMV--YPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPM 348 Query: 230 SGLIKAEMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSL-KSAGIESAFYWYV 282 + +E FP VR+ G+ ++Y + +FGG A F+ L K++G A WYV Sbjct: 349 PTAL-SEQFPTRVRSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYV 401 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 112 bits (279), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 75/217 (34%), Positives = 118/217 (54%), Gaps = 9/217 (4%) Query: 7 QGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWLTKAELM 66 QGL++GGEYG +ATY++E AP GRRG F ++ T G L++LV+ G++ + +A Sbjct: 131 QGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTAVGEAAFA 190 Query: 67 AWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQH--------KRAF 118 WGWR+PF++ + +++++R S+ E S A + K + T K L K Sbjct: 191 DWGWRIPFLVSILLLAISVWIRMSMNE-SPAFLKMKAEGKTSKAPLTEAFGQWKNLKLVI 249 Query: 119 LNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAISDK 178 L +VG TAG ++++YT Y +L T +D AN ++ AL + FG++SDK Sbjct: 250 LALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLATPFFLFFGSLSDK 309 Query: 179 IGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMAL 215 IGR+ +L V FPI L ++P + AL Sbjct: 310 IGRKPIILGGCLIAAVTYFPIFSGLTHFANPALEAAL 346 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 111 bits (278), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 86/308 (27%), Positives = 155/308 (50%), Gaps = 20/308 (6%) Query: 1 LLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWL 60 L RL QG S G E G + Y++E+A G +GF+ S+Q + + + L+ + + Q L Sbjct: 120 LTGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSL 179 Query: 61 TKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDA--GTLKGLLQHKRAF 118 + ++ WGWR+PF +G + +R SL ET ARK+ + + + Q+ + Sbjct: 180 SPVQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIV 239 Query: 119 LNVVGFTAGGSLMFYTFTTYMQ---KYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAI 175 + + ++ FY T Y K ++ + D + + + F ++ PI GA+ Sbjct: 240 VAGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWL---PIMGAL 296 Query: 176 SDKIGRRNSMLCFAFFGMVGTFPILHFL-KDVSSPGVAMALAILALTIVSFYTSISG--- 231 SD++GRR ++ F ++ +P++ +L D+S L I L + Y S +G Sbjct: 297 SDRVGRRPVLVAFTLLTLLTAYPVMSWLVSDISFKN----LLITELWLSFLYASYNGAAV 352 Query: 232 LIKAEMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSL-KSAGIESAF-YWY-VSALC-L 287 + E+ P VR +G L+Y++ AIFGG V+ L +++G ++A YW +A+C L Sbjct: 353 VALTEVMPAHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGL 412 Query: 288 VALIISLR 295 +A ++ R Sbjct: 413 LATLVLYR 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,052,289 Number of extensions: 43929 Number of successful extensions: 265 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 243 Number of HSP's successfully gapped: 13 Length of query: 331 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 245 Effective length of database: 1,212,660 Effective search space: 297101700 Effective search space used: 297101700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)