BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0050.p (457 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 924 0.0 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 212 6e-56 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 198 8e-52 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 197 1e-51 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 196 3e-51 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 924 bits (2388), Expect = 0.0, Method: Compositional matrix adjust. Identities = 457/457 (100%), Positives = 457/457 (100%) Query: 1 MSDTTQASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQ 60 MSDTTQASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQ Sbjct: 1 MSDTTQASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQ 60 Query: 61 LLNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGA 120 LLNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGA Sbjct: 61 LLNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGA 120 Query: 121 WAPALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLF 180 WAPALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLF Sbjct: 121 WAPALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLF 180 Query: 181 GMQQWLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQH 240 GMQQWLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQH Sbjct: 181 GMQQWLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQH 240 Query: 241 KRAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGA 300 KRAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGA Sbjct: 241 KRAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGA 300 Query: 301 ISDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIK 360 ISDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIK Sbjct: 301 ISDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIK 360 Query: 361 AEMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSLKSAGIESAFYWYVSALCLVALIISL 420 AEMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSLKSAGIESAFYWYVSALCLVALIISL Sbjct: 361 AEMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSLKSAGIESAFYWYVSALCLVALIISL 420 Query: 421 RMPDPQRDGHLKGDVAMNPDEHQGEADAAAGKLHKPA 457 RMPDPQRDGHLKGDVAMNPDEHQGEADAAAGKLHKPA Sbjct: 421 RMPDPQRDGHLKGDVAMNPDEHQGEADAAAGKLHKPA 457 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 212 bits (539), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 132/397 (33%), Positives = 213/397 (53%), Gaps = 18/397 (4%) Query: 2 SDTTQASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQL 61 S+T ++ + R+ AI+ +S GN +EWFD +Y A+ A FFP GD + L Sbjct: 3 SNTAAPGADITHPQARR---AIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSL 59 Query: 62 LNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGAW 121 L T F F MRP+G + G +D+ GRK ++++S+++M G+ +A MP+YA+IG Sbjct: 60 LLTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLL 119 Query: 122 APALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFG 181 APA + L ++ QG S GGE+G+S ++ E AP+ RRGFF+S+Q + LLA L Sbjct: 120 APAGIALGKMIQGFSAGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAV 178 Query: 182 MQQWLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQHK 241 + LT +L +WGWRVPF+ G + A +Y+R L E +K L+ H+ Sbjct: 179 LNNMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQ 238 Query: 242 RAFLNVVGFTAGGSLMFYTFTTYMQKYL----VNTAGMDPKVANGVMTGALFVYMILQPI 297 + L + GS++ T + Y+ Y+ V G+ + M+ A + I P+ Sbjct: 239 KMRLLI----GAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPL 294 Query: 298 FGAISDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMAL---AILALTIVSFYTS 354 GA+SDKIGR M+ + +V T+P+ FL + SPG L ++ + + ++ + Sbjct: 295 VGALSDKIGRTPFMITSSALYIVLTYPMFVFLTN--SPGFLQLLLLQTVIGVLMTMYFAA 352 Query: 355 ISGLIKAEMFPPEVRALGVGLSYAVGNAIFGGSAEFV 391 + L+ A++FP R G+ L+Y + IFGG A + Sbjct: 353 MPALL-ADIFPVATRGTGMSLAYNIAVTIFGGFAGLI 388 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 198 bits (503), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 133/427 (31%), Positives = 229/427 (53%), Gaps = 21/427 (4%) Query: 8 STPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQLLNTAGV 67 +T + A++ K + +L +SGN +E FDF++Y F A Y + FFP + L+ T G Sbjct: 2 TTTTTNAKHSK-LSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGT 60 Query: 68 FAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGAWAPALLL 127 F AGFLMRP+G + G D+ GR+ +++++ +M G+L +A +P YATIG AP L+L Sbjct: 61 FGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVL 120 Query: 128 LARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWLT 187 RL QG S G E G + Y++E+A G +GF+ S+Q + + + L+ + + Q L+ Sbjct: 121 TGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLS 180 Query: 188 KAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDA--GTLKGLLQHKRAFL 245 ++ WGWR+PF +G + +R SL ET ARK+ + + + Q+ + + Sbjct: 181 PVQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVV 240 Query: 246 NVVGFTAGGSLMFYTFTTYMQ---KYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAIS 302 + ++ FY T Y K ++ + D + + + F ++ PI GA+S Sbjct: 241 AGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWL---PIMGALS 297 Query: 303 DKIGRRNSMLCFAFFGMVGTFPILHFL-KDVSSPGVAMALAILALTIVSFYTSISG---L 358 D++GRR ++ F ++ +P++ +L D+S L I L + Y S +G + Sbjct: 298 DRVGRRPVLVAFTLLTLLTAYPVMSWLVSDISFKN----LLITELWLSFLYASYNGAAVV 353 Query: 359 IKAEMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSL-KSAGIESAF-YWY-VSALC-LV 414 E+ P VR +G L+Y++ AIFGG V+ L +++G ++A YW +A+C L+ Sbjct: 354 ALTEVMPAHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLL 413 Query: 415 ALIISLR 421 A ++ R Sbjct: 414 ATLVLYR 420 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 197 bits (501), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 124/414 (29%), Positives = 219/414 (52%), Gaps = 12/414 (2%) Query: 17 RKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQLLNTAGVFAAGFLMRP 76 + + +L +SGN +E FDF+++ F A + + AFFP + + L+ T F AGFLMRP Sbjct: 8 QSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMRP 67 Query: 77 IGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGAWAPALLLLARLFQGLS 136 +G + G D+ GR+ +++++ +M G++ +A +P+YATIGA AP L+L+ RL QG S Sbjct: 68 LGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFS 127 Query: 137 VGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWLTKAELMAWGW 196 G E G + Y++E+A G +GF+ S+Q + ++A + + + LT AE+ WGW Sbjct: 128 AGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGW 187 Query: 197 RVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAG--TLKGLLQHKRAFLNVVGFTAGG 254 R+PF +G + V +R SL ET RK + + + ++++ + + + + Sbjct: 188 RIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGMMLVSMT 247 Query: 255 SLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAISDKIGRRNSMLCF 314 ++ FY T Y + N + + + V I PI GA+SD++GRR ++ F Sbjct: 248 TVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLVTF 307 Query: 315 AFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIK---AEMFPPEVRAL 371 ++ +P +++L +S + I+ L + Y S +G + E+ P +VR Sbjct: 308 TVLTILTAYPAVNWLVHDASFA---KMLIVELWLSFLYASYNGAMVVALTEVMPADVRTA 364 Query: 372 GVGLSYAVGNAIFGGSAEFVALSLKSAGIESA---FYWYVSALC-LVALIISLR 421 G L+Y++ A+FGG +A L + A + +A+C L+A +I R Sbjct: 365 GFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYR 418 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 196 bits (499), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 133/420 (31%), Positives = 221/420 (52%), Gaps = 16/420 (3%) Query: 1 MSDTTQASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQ 60 M +T + + + ++R AI+ GN +EWFDF VYSF A+ A FFP G+ + Sbjct: 1 MQASTLPADGIVSVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTS 60 Query: 61 LLNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGA 120 LL F GF MRP+GG L G AD+ GRK ++ +++L+M G+ + + PTY +IG Sbjct: 61 LLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGL 120 Query: 121 WAPALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLF 180 +AP ++++ARL QG S GGE G++ +++E AP R +++S+ ++ LL V Sbjct: 121 FAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGT 180 Query: 181 GMQQWLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQH 240 + L A L +WGWR+PF+LG + V Y+R L ET + K K LK ++++ Sbjct: 181 FVTASLDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRN 240 Query: 241 -----KRAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGV--MTGALFVYMI 293 +F V+ +T ++ + TY K L P+ + M G L + M+ Sbjct: 241 FPRETSASFSMVILWTVCTYVLLFYMPTYSVKVL-----KLPQTTGFIAGMVGGLCI-MV 294 Query: 294 LQPIFGAISDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYT 353 L PI G ++D+IGRR + A ++ +P+ ++ S + ++ T+++ YT Sbjct: 295 LAPIVGRLADRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYT 354 Query: 354 SISGLIKAEMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSLKSA---GIESAFYWYVSA 410 +E+FP +V + G+ ++Y + IFGG A F L +A + AFY ++A Sbjct: 355 GPILAAFSELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAA 414 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,411,184 Number of extensions: 59027 Number of successful extensions: 365 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 329 Number of HSP's successfully gapped: 16 Length of query: 457 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 369 Effective length of database: 1,203,190 Effective search space: 443977110 Effective search space used: 443977110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)