BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0048.p (770 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312657|ref|YP_003776749.1| NADP-dependent malic enzyme oxi... 1570 0.0 gi|300310478|ref|YP_003774570.1| NADP-dependent malic enzyme oxi... 889 0.0 >gi|300312657|ref|YP_003776749.1| NADP-dependent malic enzyme oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 770 Score = 1570 bits (4064), Expect = 0.0, Method: Compositional matrix adjust. Identities = 770/770 (100%), Positives = 770/770 (100%) Query: 1 MDSMISKKEEVRQQLRQAALDYHEFPTPGKIAVTPTKLLTNQRDLSLAYSPGVAAACEEI 60 MDSMISKKEEVRQQLRQAALDYHEFPTPGKIAVTPTKLLTNQRDLSLAYSPGVAAACEEI Sbjct: 1 MDSMISKKEEVRQQLRQAALDYHEFPTPGKIAVTPTKLLTNQRDLSLAYSPGVAAACEEI 60 Query: 61 VADPLNAFRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGIDVFDIE 120 VADPLNAFRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGIDVFDIE Sbjct: 61 VADPLNAFRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGIDVFDIE 120 Query: 121 VNETDPDKLIDVIAALEPTFGGINLEDIKAPECFYIERKLREKMKIPVFHDDQHGTAIIV 180 VNETDPDKLIDVIAALEPTFGGINLEDIKAPECFYIERKLREKMKIPVFHDDQHGTAIIV Sbjct: 121 VNETDPDKLIDVIAALEPTFGGINLEDIKAPECFYIERKLREKMKIPVFHDDQHGTAIIV 180 Query: 181 GAAILNGLKVVGKDIKNVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRK 240 GAAILNGLKVVGKDIKNVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRK Sbjct: 181 GAAILNGLKVVGKDIKNVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRK 240 Query: 241 ELMDPDKERFARETEARTLAEVIPDADIFLGLSAAGVLKPEMVKQMAARPLVLALANPTP 300 ELMDPDKERFARETEARTLAEVIPDADIFLGLSAAGVLKPEMVKQMAARPLVLALANPTP Sbjct: 241 ELMDPDKERFARETEARTLAEVIPDADIFLGLSAAGVLKPEMVKQMAARPLVLALANPTP 300 Query: 301 EILPEEVKAVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIATVHAI 360 EILPEEVKAVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIATVHAI Sbjct: 301 EILPEEVKAVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIATVHAI 360 Query: 361 AELAQAEQSDIVATTYGIANLSFGPEYIIPKPFDPRLMIKIAPAVARAAESSGVAARPIQ 420 AELAQAEQSDIVATTYGIANLSFGPEYIIPKPFDPRLMIKIAPAVARAAESSGVAARPIQ Sbjct: 361 AELAQAEQSDIVATTYGIANLSFGPEYIIPKPFDPRLMIKIAPAVARAAESSGVAARPIQ 420 Query: 421 DMDAYIEKLEQFVYHSGTFMRPIFQVAKKAAEAKKRIVYAEGEEERVLRAVQVIVDENLA 480 DMDAYIEKLEQFVYHSGTFMRPIFQVAKKAAEAKKRIVYAEGEEERVLRAVQVIVDENLA Sbjct: 421 DMDAYIEKLEQFVYHSGTFMRPIFQVAKKAAEAKKRIVYAEGEEERVLRAVQVIVDENLA 480 Query: 481 KPILVGRPEVLEQRIQKFGLRLRAGTDFEVINPNFDNRYRDYWQTFLEMSRRKGVTEQYA 540 KPILVGRPEVLEQRIQKFGLRLRAGTDFEVINPNFDNRYRDYWQTFLEMSRRKGVTEQYA Sbjct: 481 KPILVGRPEVLEQRIQKFGLRLRAGTDFEVINPNFDNRYRDYWQTFLEMSRRKGVTEQYA 540 Query: 541 KLEMRRRHTLIGSMAIHKGDADGMICGTYGTTQLHLHYIDQVLGKREGVNVYAAMNAVIL 600 KLEMRRRHTLIGSMAIHKGDADGMICGTYGTTQLHLHYIDQVLGKREGVNVYAAMNAVIL Sbjct: 541 KLEMRRRHTLIGSMAIHKGDADGMICGTYGTTQLHLHYIDQVLGKREGVNVYAAMNAVIL 600 Query: 601 PNRQLVMVDTHVNENPNARELAEITMLAAEEMRRFGLHPRAALLSHSNFGSSNSESARKM 660 PNRQLVMVDTHVNENPNARELAEITMLAAEEMRRFGLHPRAALLSHSNFGSSNSESARKM Sbjct: 601 PNRQLVMVDTHVNENPNARELAEITMLAAEEMRRFGLHPRAALLSHSNFGSSNSESARKM 660 Query: 661 REALAILREIAPELEVDGEMHGDTALDSKLLNAVIPDSPLKGDANLLVMPNIDAANIAYN 720 REALAILREIAPELEVDGEMHGDTALDSKLLNAVIPDSPLKGDANLLVMPNIDAANIAYN Sbjct: 661 REALAILREIAPELEVDGEMHGDTALDSKLLNAVIPDSPLKGDANLLVMPNIDAANIAYN 720 Query: 721 LLKTASGNGVAIGPILLGCAKPVHILTPSATVRRIINMTALCVMDALSER 770 LLKTASGNGVAIGPILLGCAKPVHILTPSATVRRIINMTALCVMDALSER Sbjct: 721 LLKTASGNGVAIGPILLGCAKPVHILTPSATVRRIINMTALCVMDALSER 770 >gi|300310478|ref|YP_003774570.1| NADP-dependent malic enzyme oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 758 Score = 889 bits (2297), Expect = 0.0, Method: Compositional matrix adjust. Identities = 453/767 (59%), Positives = 568/767 (74%), Gaps = 17/767 (2%) Query: 1 MDSMISKKEEVRQQLRQAALDYHEFPTPGKIAVTPTKLLTNQRDLSLAYSPGVAAACEEI 60 M IS E Q LR AA +YH P+ GKI V PTK L+NQRDLSLAYSPGVA C +I Sbjct: 1 MTQQISAAE---QLLRDAAREYHRTPSRGKIEVRPTKPLSNQRDLSLAYSPGVAYPCLDI 57 Query: 61 VADPLNAFRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGIDVFDIE 120 ADPL AF YT+RGNLVAVISNGTAVLGLGNIG LASKPVMEGKG LFKKFAG+DVFDIE Sbjct: 58 EADPLKAFDYTSRGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGCLFKKFAGVDVFDIE 117 Query: 121 VNETDPDKLIDVIAALEPTFGGINLEDIKAPECFYIERKLREKMKIPVFHDDQHGTAIIV 180 + E DPDKL+++IA+LEPT GGINLEDIKAPECFYIE+KL +M IPVFHDDQHGTAII Sbjct: 118 LAENDPDKLVEIIASLEPTLGGINLEDIKAPECFYIEQKLSARMNIPVFHDDQHGTAIIS 177 Query: 181 GAAILNGLKVVGKDIKNVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRK 240 AA+LNGL++VGKDI VK+ VSGAGAAA+AC+++++ LG EN+Y+ D GV+Y+GR Sbjct: 178 SAALLNGLELVGKDIGKVKIAVSGAGAAAIACVEVMIGLGVKRENVYMVDSKGVIYEGRP 237 Query: 241 ELMDPDKERFARETEARTLAEVIPDADIFLGLSAAGVLKPEMVKQMAARPLVLALANPTP 300 +D K+R+A +TEARTLA+V+ AD+FLG SA GVL EMVK MA +P++LALANP P Sbjct: 238 GGLDASKQRYAIKTEARTLADVVNGADVFLGCSAPGVLSAEMVKTMADKPIILALANPEP 297 Query: 301 EILPEEVKAVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIATVHAI 360 EI PE K+VR D IIATGRSDYPNQVNNVLCFPYIFRGALD GAT IT M++A V I Sbjct: 298 EIRPELAKSVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVREI 357 Query: 361 AELAQAEQSDIVATTYGIANLSFGPEYIIPKPFDPRLMIKIAPAVARAAESSGVAARPIQ 420 A+LA+AE S V+ Y L FG +Y+IP PFD RL++KIAPAVA+AA SGVA RPI+ Sbjct: 358 ADLAKAETSAEVSAAYPGKELVFGADYLIPTPFDSRLILKIAPAVAKAAAESGVATRPIK 417 Query: 421 DMDAYIEKLEQFVYHSGTFMRPIFQVAKKAAEAKKRIVYAEGEEERVLRAVQVIVDENLA 480 D DAY +L +F+Y +G FM+P+F AK A + R+VYA+G++ERVLRAVQ + DE LA Sbjct: 418 DYDAYRAELTRFIYQTGMFMQPVFNTAKVAPQ---RVVYADGQDERVLRAVQFVADEGLA 474 Query: 481 KPILVGRPEVLEQRIQKFGLRLRAGTDFEVINPNFDNRYRDYWQTFLEMSRRKGVTEQYA 540 PILVG P +E I+K GLR++ G + +VI+P D R Y + + Sbjct: 475 APILVGAPAAIEAGIKKAGLRIKIGQNVQVIDPATDARVAQYAAQY----------KDGG 524 Query: 541 KLEMRRRHTLIGSMAIHKGDADGMICGTYGTTQLHLHYIDQVLGKREGVNVYAAMNAVIL 600 + ++R +L+ ++ + GDAD M+CG G HL ++++V+G ++G +AA+NAV+L Sbjct: 525 EADVRASGSLLAALMVKAGDADAMLCGLDGNYDGHLAHVEKVIGLKQGAQEFAALNAVLL 584 Query: 601 PNRQLVMVDTHVNENPNARELAEITMLAAEEMRRFGLHPRAALLSHSNFGSSNSESARKM 660 P++ L + DT+VN+ P+A +LA I AAEE+ RFG+ P+ A LSHSN GSS+ SARKM Sbjct: 585 PDQTLFITDTYVNDTPDAEQLARIAQAAAEEVARFGVPPKVAFLSHSNRGSSSRPSARKM 644 Query: 661 REALAILREIAPELEVDGEMHGDTALDSKLL-NAVIPDSPLKGDANLLVMPNIDAANIAY 719 A +++APE+E GE+HGD AL K+ NA + DS L G AN+LV PN+DAANI + Sbjct: 645 AAAHEAFKKLAPEVESAGELHGDAALSEKIRNNAGLADSGLTGAANVLVCPNLDAANILF 704 Query: 720 NLLKTASGNGVAIGPILLGCAKPVHILTPSATVRRIINMTALCVMDA 766 N+LKT+SG G IGPILLG A PVH+LTPS+TVRR+INMTAL A Sbjct: 705 NVLKTSSGQGTTIGPILLGAAAPVHVLTPSSTVRRVINMTALAAAKA 751 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,284,702 Number of extensions: 95654 Number of successful extensions: 341 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 337 Number of HSP's successfully gapped: 2 Length of query: 770 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 677 Effective length of database: 1,179,515 Effective search space: 798531655 Effective search space used: 798531655 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)