BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0032.p (920 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312669|ref|YP_003776761.1| Mg(2+) transport ATPase [Herbas... 1888 0.0 gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbas... 280 3e-76 gi|300313072|ref|YP_003777164.1| high-affinity K+ transport syst... 80 7e-16 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 77 8e-15 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 76 9e-15 >gi|300312669|ref|YP_003776761.1| Mg(2+) transport ATPase [Herbaspirillum seropedicae SmR1] Length = 920 Score = 1888 bits (4891), Expect = 0.0, Method: Compositional matrix adjust. Identities = 917/920 (99%), Positives = 919/920 (99%) Query: 1 MNLNLLKETFVSFIRARGMDRHFRRLGMDMFRSAPVTKEVPQTLAEQLSDASRSHAQDLL 60 MNLNLLKETFVSFIRARGMDRHFRRLGMDMFRSAPVTKEVPQTLAEQLSDASRSH QDLL Sbjct: 1 MNLNLLKETFVSFIRARGMDRHFRRLGMDMFRSAPVTKEVPQTLAEQLSDASRSHVQDLL 60 Query: 61 QRLHSRPDGLTESEADAIRAHSGPNEVEHEKPLTWYVHLWHCYKNPFNLLLTLLAAVSFY 120 QRLHSRPDGLTESEADAIRAHSGPNEVEHEKPLTWYVHLWHCYKNPFNLLLTLLAAVSFY Sbjct: 61 QRLHSRPDGLTESEADAIRAHSGPNEVEHEKPLTWYVHLWHCYKNPFNLLLTLLAAVSFY 120 Query: 121 TEDMKAAIVIGTMVVISTLLRFVQEARSNTAADKLKAMVSNTATVMRHDQVEEIAAEAQR 180 TEDMKAAIVIGTMVV+STLLRFVQEARSNTAADKLKAMVSNTATVMRHDQVEEIAAEAQR Sbjct: 121 TEDMKAAIVIGTMVVVSTLLRFVQEARSNTAADKLKAMVSNTATVMRHDQVEEIAAEAQR 180 Query: 181 YFDVTLHPKGARRVEVPIRQLVPGDIVLLSAGDMIPADLRLLAAKDLFVSQAAMTGESLP 240 YFDVTLHPKGARRVEVPIRQLVPGDIVLLSAGDMIPADLRLLAAKDLFVSQAAMTGESLP Sbjct: 181 YFDVTLHPKGARRVEVPIRQLVPGDIVLLSAGDMIPADLRLLAAKDLFVSQAAMTGESLP 240 Query: 241 VEKFVTPSNLNTSSPLELDNLCFMGTNVVSGSATGVVLTTGKRTYFGALAERVTATDRTP 300 VEKFVTPSNLNTSSPLELDNLCFMGTNVVSGSATGVVLTTGKRTYFGALAERVTATDRTP Sbjct: 241 VEKFVTPSNLNTSSPLELDNLCFMGTNVVSGSATGVVLTTGKRTYFGALAERVTATDRTP 300 Query: 301 TAFQAGVNKVSWLLIRFMLVMTPVVFFINGFTKGDWVEAFLFGLSVAVGLTPEMLPMIVT 360 TAFQAGVNKVSWLLIRFMLVMTPVVFFINGFTKGDWVEAFLFGLSVAVGLTPEMLPMIVT Sbjct: 301 TAFQAGVNKVSWLLIRFMLVMTPVVFFINGFTKGDWVEAFLFGLSVAVGLTPEMLPMIVT 360 Query: 361 STLAKGAVALSRKKVIVKRLDAIQNFGAMDVLCTDKTGTLTQDKIFLERHTDIHGEPDDE 420 STLAKGAVALSRKKVIVKRLDAIQNFGAMDVLCTDKTGTLTQDKIFLERHTDIHGEPDDE Sbjct: 361 STLAKGAVALSRKKVIVKRLDAIQNFGAMDVLCTDKTGTLTQDKIFLERHTDIHGEPDDE 420 Query: 421 VLQYAYLNSYYQTGLKNLLDVAVLEHAELQREMSIASAYRKVDEIPFDFQRRRMSVVVSE 480 VLQYAYLNSYYQTGLKNLLDVAVLEHAELQREMSIASAYRKVDEIPFDFQRRRMSVVVSE Sbjct: 421 VLQYAYLNSYYQTGLKNLLDVAVLEHAELQREMSIASAYRKVDEIPFDFQRRRMSVVVSE 480 Query: 481 REDHHELICKGAVEEIVSVCSRARVNGEVVPMTPALLEEIRATTGSLNAEGLRVVGVAAK 540 REDHHELICKGAVEEIVSVCSRARVNGEVVPMTPALLEEIRATTGSLNAEGLRVVGVAAK Sbjct: 481 REDHHELICKGAVEEIVSVCSRARVNGEVVPMTPALLEEIRATTGSLNAEGLRVVGVAAK 540 Query: 541 DLPPTKEVYSLADESDLVLIGYIAFLDPPKESTAPALAALRAHGVKVKILTGDNELVTAK 600 DLPPTKEVYSLADESDLVLIGYIAFLDPPKESTAPALAALRAHGVKVKILTGDNELVTAK Sbjct: 541 DLPPTKEVYSLADESDLVLIGYIAFLDPPKESTAPALAALRAHGVKVKILTGDNELVTAK 600 Query: 601 ICREVGLEVKGMLLGSYVEKMSDAELSEAVETVTVFAKLSPAHKERIVRVLHDRGHVVGF 660 ICREVGLEVKGMLLGSYVEKMSDAELSEAVETVTVFAKLSPAHKERIVRVLHDRGHVVGF Sbjct: 601 ICREVGLEVKGMLLGSYVEKMSDAELSEAVETVTVFAKLSPAHKERIVRVLHDRGHVVGF 660 Query: 661 MGDGINDAPALRAADIGISVDTAVDIAKEAADLILLEKSLMVLEEGVLEGRKTFANMLKY 720 MGDGINDAPALRAADIGISVDTAVDIAKEAADLILLEKSLMVLEEGVLEGRKTFANMLKY Sbjct: 661 MGDGINDAPALRAADIGISVDTAVDIAKEAADLILLEKSLMVLEEGVLEGRKTFANMLKY 720 Query: 721 IKMTASSNFGNVFSVLIASAFLPFLPMLPLHLLVQNLLYDVSQISIPFDNVDKEFLEKPQ 780 IKMTASSNFGNVFSVLIASAFLPFLPMLPLHLLVQNLLYDVSQISIPFDNVDKEFLEKPQ Sbjct: 721 IKMTASSNFGNVFSVLIASAFLPFLPMLPLHLLVQNLLYDVSQISIPFDNVDKEFLEKPQ 780 Query: 781 RWDAGDIGRFMVFFGPISSIFDITTFALMWFIFGASSPEHQTLFQSGWFIEGLLSQTLIV 840 RWDAGDIGRFMVFFGPISSIFDITTFALMWFIFGASSPEHQTLFQSGWFIEGLLSQTLIV Sbjct: 781 RWDAGDIGRFMVFFGPISSIFDITTFALMWFIFGASSPEHQTLFQSGWFIEGLLSQTLIV 840 Query: 841 HMIRTRRIPFFQSRASWPLMGMTLIIMAIGIAIPMTPLAHYFKLEALPLSYFPWLVGILV 900 HMIRTRRIPFFQSRASWPLMGMTLIIMAIGIAIPMTPLAHYFKLEALPLSYFPWLVGILV Sbjct: 841 HMIRTRRIPFFQSRASWPLMGMTLIIMAIGIAIPMTPLAHYFKLEALPLSYFPWLVGILV 900 Query: 901 AYAVLIQGMKGWYARRYGWQ 920 AYAVLIQGMKGWYA+RYGWQ Sbjct: 901 AYAVLIQGMKGWYAKRYGWQ 920 >gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbaspirillum seropedicae SmR1] Length = 853 Score = 280 bits (717), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 217/775 (28%), Positives = 377/775 (48%), Gaps = 75/775 (9%) Query: 69 GLTESEADAIRAHSGPNEVEHEKPLTWYVHLWHCYKNPFNLLLTLLAAVSFYTEDMKAAI 128 GL+ SE + A GPN + ++ V L + P +LL AA+ +++ + Sbjct: 11 GLSHSEVTRLLAEDGPNVLHSDQRRMGRV-LMKVIREPMFMLLLSAAAIYLTLGELREGL 69 Query: 129 VIGTMVVISTLLRFVQEARSNTAADKLKAMVSNTATVMRHDQVEEIAAEAQRYFDVTLHP 188 ++ MV ++ L Q ++ A L+ + S TA V+R + IAA Sbjct: 70 LLLFMVALTVGLTLYQGGKTERALAALRVLNSPTARVIRDGRTMRIAAA----------- 118 Query: 189 KGARRVEVPIRQLVPGDIVLLSAGDMIPADLRLLAAKDLFVSQAAMTGESLPVEKFVTPS 248 +LV GD++LL G +PAD +L A +L + ++ +TGE+ PVEKF + Sbjct: 119 -----------ELVRGDLILLEEGARVPADAGVLEASNLQLDESLLTGEACPVEKFAPST 167 Query: 249 NLNT----SSPLELDNLCFMGTNVVSGSATGVVLTTGKRTYFGALAERVTATDRTPTAFQ 304 S + L GT VV GS V TG RT G ++ + P+ Q Sbjct: 168 KPEPHALPSGQTDSATLVRSGTLVVRGSGIAKVTATGARTELGKISSLLHELVEEPSPMQ 227 Query: 305 AGVNKVSWLLIRFMLVMTPVVFFINGFTKGDWVEAFLFGLSVAVGLTPEMLPMIVTSTLA 364 + ++ +L L+++ ++ + G++ G W EA L G+++++ L PE LP+I+T A Sbjct: 228 RHIARIVTVLAVTGLMLSALLVVLEGYSHGRWTEALLMGIALSMSLLPEELPIILTIFPA 287 Query: 365 KGAVALSRKKVIVKRLDAIQNFGAMDVLCTDKTGTLTQDKI-------------FLERHT 411 GA ++R V+ +RL AI+ G++ VLCTDKTGTLT++++ F E Sbjct: 288 IGAWRMARANVLTRRLSAIETLGSISVLCTDKTGTLTENRMRVVQGMIAGRHMYFDESDI 347 Query: 412 DIHGEPDDEVLQYAYLNSYYQTGLKNLLDVAVLEHA-ELQREMSIASAYRKVDEIPFDFQ 470 I +L+ A L S + + ++ A + A E + + + + + E PF Sbjct: 348 GITSPEWRTLLETAALASSPRP--TDPMEKAFHQLAGESAKRFAPPALTQLLQEYPFTPA 405 Query: 471 RRRMSVVVSEREDHHELIC-KGAVEEIVSVCSRARVNGEVVPMTPALLEEIRATTGSLNA 529 R M++V +D L+ KGA E I S+C +TPA +++++ L + Sbjct: 406 LRAMTMVWKGDDDAPLLVATKGAPEAIASLCG----------LTPAEVQQMQNDIMHLGS 455 Query: 530 EGLRVVGVAAKD----LPPTKEVYSLADESDLVLIGYIAFLDPPKESTAPALAALRAHGV 585 GLRV+ VAA + LP ++ + L +G +A DP + A+ + Sbjct: 456 HGLRVIAVAACNWSGALPASQAQFGLR------YLGLLALADPLRPGVPDAIQRCHQAEI 509 Query: 586 KVKILTGDNELVTAKICREVGLEVKGMLLGSYVEKMSDAELSEAVETVTVFAKLSPAHKE 645 +V ++TGD+ I R+ G+ +LLG V+ M++ EL+ + T V A+++P K Sbjct: 510 RVIMITGDHPSTAQNIARQAGISADQVLLGDEVQAMAEHELARRLRTTNVCARITPLQKL 569 Query: 646 RIVRVLHDRGHVVGFMGDGINDAPALRAADIGISVDT-AVDIAKEAADLILLEKSLMVLE 704 RIV L D G ++ GDG+NDAPAL+AA +GI++ D+A+EA LILL+ + + Sbjct: 570 RIVAALKDDGEIIAMTGDGVNDAPALKAAHVGIAMGARGTDVAREAGSLILLDDNFASIV 629 Query: 705 EGVLEGRKTFANMLKYIKMTASSNFGNVFSVLIASAFLPFLPMLPLHLLVQNLLYDVSQI 764 EG+L GR+ F+ M + + + L+ F L + P+ + L+ D + Sbjct: 630 EGILMGRRIFSRMRGAMIYVLAMHMPIAGMALLPVVFGWPLILYPMQIAFLELIVDPA-C 688 Query: 765 SIPFDNVDKEFLEK--PQRWDAGDI--GRFMVFF---GPISSIFDITTFALMWFI 812 ++ F+N ++ E+ P+R A + R ++ G ++ +F ++ + +WF+ Sbjct: 689 TLAFENTGSDYRERQAPRRHGAAPLLDARIIILVLVQGSVALLFAVSAY--LWFL 741 >gi|300313072|ref|YP_003777164.1| high-affinity K+ transport system ATPase [Herbaspirillum seropedicae SmR1] Length = 730 Score = 80.1 bits (196), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 137/554 (24%), Positives = 223/554 (40%), Gaps = 71/554 (12%) Query: 204 GDIVLLSAGDMIPADLRLLAAKDLFVSQAAMTGESLPVEKFVTPSNLNTSSPLELDNLCF 263 GD+VL+ AGD +PAD ++ V ++A+TGES PV + S + S+ Sbjct: 159 GDLVLIEAGDTVPADAEVIEGVAT-VDESAITGESAPV---IRESGGDFSA-------VT 207 Query: 264 MGTNVVSGSATGVVLTTGKRTYFGALAERVTATDRTPTAFQAGVNKVSWLLIRFMLVMTP 323 GT V+S V + + V R T + + + L L++T Sbjct: 208 GGTRVLSDWIVVRVSVNPGEAFLDRMIAMVEGARRQKTPNELALTLLLVALTLVFLLVTV 267 Query: 324 V-----VFFINGFTKGDWVEAFLFGLSVAVGLTPEMLPMIVTSTLAKGAVALSRKKVIVK 378 +F +N G V +++ V L P + ++++ G + + VI Sbjct: 268 TLLPFSIFSVNAAGSGSPVSVTAL-IALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIAT 326 Query: 379 RLDAIQNFGAMDVLCTDKTGTLT----QDKIFLERHTDIHGEPDDEVLQYAYLNSYYQTG 434 A++ G +DVL DKTGT+T Q F+ E D V Q A L G Sbjct: 327 SGRAVEAAGDVDVLLLDKTGTITLGNRQAAAFMPAPGVTERELAD-VAQLASLADETPEG 385 Query: 435 LKNLLDVAVLEHAELQREMSIASAYRKVDEIPFDFQRRRMSVVVSEREDHHELICKGAVE 494 ++++ +A +REM+ A + IPF Q R VS + H I KGA + Sbjct: 386 -RSIVVLAKNRFNLREREMANLGA----EFIPFSAQTR-----VSGIDLGHRRIRKGAAD 435 Query: 495 EIVSVCSRARVNGEVVPMTPALLEEIRATTGSLNAEGLRVVGVAAKDLPPTKEVYSLADE 554 I + A PA +E + L V+ A E + Sbjct: 436 AIRKLTRAADQR------LPAQVESDIESIARRGGTPLVVIEWHA-------EAAMINQG 482 Query: 555 SDLVLIGYIAFLDPPKESTAPALAALRAHGVKVKILTGDNELVTAKICREVGLEVKGMLL 614 + + ++G + D K LR G+K ++TGDN L A I E G+ Sbjct: 483 APIRILGTVELKDIVKGGIKERFGELRRMGIKTVMITGDNRLTAAAIAAEAGV------- 535 Query: 615 GSYVEKMSDAELSEAVETVTVFAKLSPAHKERIVRVLHDRGHVVGFMGDGINDAPALRAA 674 D L+EA +P K ++R G +V GDG NDAPAL A Sbjct: 536 --------DDFLAEA----------TPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQA 577 Query: 675 DIGISVDTAVDIAKEAADLILLEKSLMVLEEGVLEGRKTFANMLKYIKMTASSNFGNVFS 734 D+ +++++ AKEA +++ L+ + L E V G++ + +++ F+ Sbjct: 578 DVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFA 637 Query: 735 VLIASAFLPFLPML 748 + I + F+ P L Sbjct: 638 I-IPAMFVGSYPQL 650 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 76.6 bits (187), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 49/170 (28%), Positives = 85/170 (50%), Gaps = 26/170 (15%) Query: 553 DESDLVLIGYIAFLDPPKESTAPALAALRAHGVKVKILTGDNELVTAKICREVGLEVKGM 612 ++S + ++G +AF D K A+A L GV+ +L+GDN ++ E+G++ Sbjct: 545 NDSGVEVLGLLAFGDQIKPGAQAAIATLHRMGVRSAMLSGDNLGAAQQVASELGIQ---- 600 Query: 613 LLGSYVEKMSDAELSEAVETVTVFAKLSPAHKERIVRVLHDRG-HVVGFMGDGINDAPAL 671 V A++ PA K V L G VV +GDG+NDAPAL Sbjct: 601 ---------------------EVRAEVLPADKAEAVTALRQGGAQVVAMVGDGVNDAPAL 639 Query: 672 RAADIGISVDTAVDIAKEAADLILLEKSLMVLEEGVLEGRKTFANMLKYI 721 AAD+G+++ T D+A +AA + L+ ++ + + R+T+A + + + Sbjct: 640 AAADVGMAMSTGTDVAMQAAGVTLMHGDPGLVADAIDISRRTYAKIRQNL 689 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 76.3 bits (186), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 134/540 (24%), Positives = 215/540 (39%), Gaps = 105/540 (19%) Query: 196 VPIRQLVPGDIVLLSAGDMIPADLRLLAAKDLFVSQAAMTGESLPVEKF----VTPSNLN 251 VP R + D V ++ G+ +P D L+ + ++QA +TGES+PV+K V +N Sbjct: 281 VPARTVTVDDTVRVAPGERVPLD-GLVLSGSTSINQAPITGESIPVQKQAGDPVFAGTIN 339 Query: 252 TSSPLELDNLCFMGTNVVSGSATGVVLTTGKRTYFGALAERVTATDRTPTAFQAGVNKVS 311 E+ G + ++ V G+R PT Q V+ + Sbjct: 340 EQGAFEMRVTAAQGDSTLARIVKSVQQAQGQRA---------------PT--QRFVDNFA 382 Query: 312 WLLIRFMLVMTPVVFFINGFTKGDWVEAFLF-GLSVAVGLTPEMLPMIVTSTLAKGAVAL 370 I ++VM +V + G + +L+ L + V P L + T+ G + Sbjct: 383 RYYIPAVVVMAVLVALLPPLLAGAAFQPWLYKALVLLVIACPCALVISTPVTIVSGLASA 442 Query: 371 SRKKVIVKRLDAIQNFGAMDVLCTDKTGTLTQDKIFLERHTDIHGEPDDEVLQYAYLNSY 430 +R ++VK ++ + L DKTGTLT K + + + D + L Sbjct: 443 ARHGILVKGGVYLEQGRQLRALALDKTGTLTFGKPRVTDVVSLQAQADQQAL-------- 494 Query: 431 YQTGLKNLLDVAVLEHAELQREMSIASAYRKVDEIPFDFQRRRMSVVVSEREDHHELICK 490 LQ +S+A+ R VS E I Sbjct: 495 ------------------LQLAVSLAA---------------RSDHPVSRAIAAQEEIAT 521 Query: 491 GAVEEIVSVCSRA---RVNGEVVPM-TPALLEEIRATTGSLNAEGLRVVGVAAKDLPPTK 546 V E ++ R R+NG+++ + L+ E+ + L A L+ K T Sbjct: 522 VEVSEFAALAGRGVQGRINGQLLHLGNHRLVHELGQCSPELEAR-LQAFEKQGK----TT 576 Query: 547 EVYSLADESDLVLIGYIAFLDPPKESTAPALAALRAHGVKVKILTGDNELVTAKICREVG 606 V L + L+ +A D + S+ A+A LRA GV+V +LTGDN I + G Sbjct: 577 TVLCLDGQPQLL----VAVADTVRPSSRQAVAELRALGVEVIMLTGDNSHTAQAIAAQTG 632 Query: 607 LEVKGMLLGSYVEKMSDAELSEAVETVTVFAKLSPAHKERIVRVLHDRGHV--VGFMGDG 664 + SDA + P K + + L R V VG +GDG Sbjct: 633 I--------------SDARGDQL-----------PEDKLKAIEELSQRHGVRGVGMVGDG 667 Query: 665 INDAPALRAADIGISVDTA-VDIAKEAADLILLEKSLMVLEEGVLEGRKTFANMLKYIKM 723 INDAPAL ADIG ++ A D A E AD+ L++ L + + + RK A +++ I + Sbjct: 668 INDAPALARADIGFAMGAAGTDTALETADVALMDDDLRKIPDFIRLSRKAHAVLVQNISI 727 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,644,101 Number of extensions: 107976 Number of successful extensions: 376 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 363 Number of HSP's successfully gapped: 8 Length of query: 920 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 826 Effective length of database: 1,174,780 Effective search space: 970368280 Effective search space used: 970368280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)