BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0025.p (211 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 410 e-116 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 94 9e-21 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 87 7e-19 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 76 2e-15 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 63 2e-11 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 410 bits (1053), Expect = e-116, Method: Compositional matrix adjust. Identities = 208/211 (98%), Positives = 209/211 (99%) Query: 1 VSELPLPELAARPIEAIVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQ 60 +SELPLPELAARPIEAIVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQ Sbjct: 1 MSELPLPELAARPIEAIVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQ 60 Query: 61 IFADRCRQPVVEAQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAID 120 IFADRCRQPVVEAQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAID Sbjct: 61 IFADRCRQPVVEAQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAID 120 Query: 121 VLFESAADVYGPRLLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAM 180 VLFESAADVYGPRLLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAM Sbjct: 121 VLFESAADVYGPRLLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAM 180 Query: 181 GNIDYVLTLQQIADPYRTLGASGLAQEKERR 211 GNIDYVLTLQQIAD RTLGASGLAQEKERR Sbjct: 181 GNIDYVLTLQQIADLLRTLGASGLAQEKERR 211 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 94.0 bits (232), Expect = 9e-21, Method: Compositional matrix adjust. Identities = 68/194 (35%), Positives = 93/194 (47%), Gaps = 5/194 (2%) Query: 17 IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQPVVEAQDK 76 I+IGAS GG EA+ E L + ++ H+P AQ + C+ VVE+ Sbjct: 167 IIIGASTGGTEAIKEFLIRMPSDCP-GILITQHMPEGFTRSFAQRLNNLCKISVVESAGN 225 Query: 77 EPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLFESAADVYGPRLLG 136 E G + AP HL + RS + + VN RP++DVLF+SAA G +G Sbjct: 226 ERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVG 285 Query: 137 ILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQQIADPY 196 ++LTG DGA GM + AG Q V MP +A+A+G VL LQ A P Sbjct: 286 VILTGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQ--AMPG 343 Query: 197 RTLGASGLAQEKER 210 R L + LA+ R Sbjct: 344 RVL--AWLAEHGSR 355 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 87.4 bits (215), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 66/195 (33%), Positives = 98/195 (50%), Gaps = 11/195 (5%) Query: 5 PLPELAARPIEA--IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIF 62 P PE RP A + IGAS GG +AL +L L + +VV H+P+ + F Sbjct: 183 PGPEDGLRPATARIVAIGASTGGPQALERVLGKLSQRCP-GIVVVQHMPQR----FTRSF 237 Query: 63 ADRCRQ----PVVEAQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPA 118 A+R + V EA+ + G + AP HL+V R + + LV+ +P+ Sbjct: 238 AERLHRISGAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPS 297 Query: 119 IDVLFESAADVYGPRLLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQAL 178 +DVLF S A G +GI++TG DGA GM+ +A GG T Q + V MP +A+ Sbjct: 298 VDVLFRSLAKAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAI 357 Query: 179 AMGNIDYVLTLQQIA 193 G + V+TL+ I+ Sbjct: 358 QQGGVGDVITLEHIS 372 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 76.3 bits (186), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 56/177 (31%), Positives = 92/177 (51%), Gaps = 3/177 (1%) Query: 17 IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQPVVEAQDK 76 + +GASAGG AL+ +L A+ +G A A ++V H+ +AQ + V AQ+ Sbjct: 161 VAVGASAGGPAALATLLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEG 220 Query: 77 EPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLFESAADVYGPRLLG 136 + Q G + A + HL+V ++G L + RP++DV F+S + ++ G Sbjct: 221 QAPQRGAVLLAASNRHLVV---KEGGRLGYVEGAPNDVYRPSVDVFFQSIVRHWPGKVAG 277 Query: 137 ILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQQIA 193 +LLTG DGAAG++A+ +G T+ Q + V MP A+A+ VL + +A Sbjct: 278 LLLTGMGRDGAAGLKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMA 334 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 63.2 bits (152), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 52/183 (28%), Positives = 77/183 (42%), Gaps = 1/183 (0%) Query: 17 IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQPVVEAQDK 76 ++IG S GG L EILPAL +V H+P A+ C V+E Sbjct: 167 VLIGVSTGGPSTLEEILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAP 226 Query: 77 EPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLFESAADVYGPR-LL 135 Q G ++ A +LV + + + + P++++L SA R L+ Sbjct: 227 TVVQGGHVYIARGGADMLVAKRGSHLSVLPKPESPTHLWHPSVELLARSALQSMAARQLI 286 Query: 136 GILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQQIADP 195 G++LTG +DGAA I GG T+ +S V MP + G VL +IA Sbjct: 287 GVMLTGMGNDGAAAFAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQ 346 Query: 196 YRT 198 R Sbjct: 347 LRV 349 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 722,293 Number of extensions: 31035 Number of successful extensions: 132 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 128 Number of HSP's successfully gapped: 5 Length of query: 211 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 130 Effective length of database: 1,236,335 Effective search space: 160723550 Effective search space used: 160723550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)