BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0020.p (459 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 932 0.0 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 211 9e-56 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 185 6e-48 gi|300314014|ref|YP_003778106.1| two component sensor histidine ... 171 1e-43 gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase tra... 169 4e-43 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 932 bits (2409), Expect = 0.0, Method: Compositional matrix adjust. Identities = 456/459 (99%), Positives = 458/459 (99%) Query: 1 MTATHSSIRRDLLKWLIAPLLLVNLIGAGLTYWLAWLPAQHAFDQNLMDSTWGLYAQVRH 60 MTA HSSIRRDLLKWLIAPLLLVNLIGAGLTYWLAWLPAQHAFDQNLMDSTWGLYAQVRH Sbjct: 1 MTAMHSSIRRDLLKWLIAPLLLVNLIGAGLTYWLAWLPAQHAFDQNLMDSTWGLYAQVRH 60 Query: 61 RQERTTAELTQQAEQILRSNHSDTTFFAVRNSEGQIVVGDKGFPQLPASDVFDRPINYDG 120 RQERTTAELTQQAEQILRSNHSDTTFFAVRN+EGQIVVGDKGFPQLPASDVFDRPINYDG Sbjct: 61 RQERTTAELTQQAEQILRSNHSDTTFFAVRNTEGQIVVGDKGFPQLPASDVFDRPINYDG 120 Query: 121 EMRGEPVRIAAMQVRVKNGVIWVAVAETIRKRDRAHYTILLSFLVLDGALTFASIFVVLV 180 EMRGEPVRIAAMQVRVKNGVIWVAVAETIRKRDRAHYTILLSFLVLDGALTFASIFVVLV Sbjct: 121 EMRGEPVRIAAMQVRVKNGVIWVAVAETIRKRDRAHYTILLSFLVLDGALTFASIFVVLV 180 Query: 181 AVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGEQAQQNF 240 AVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGEQAQQNF Sbjct: 181 AVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGEQAQQNF 240 Query: 241 MADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALARSE 300 MADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALARSE Sbjct: 241 MADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALARSE 300 Query: 301 PGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDLQPARLRGDRFLLSDLIDNLI 360 PGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDL+PARLRGDRFLLSDLIDNLI Sbjct: 301 PGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDLRPARLRGDRFLLSDLIDNLI 360 Query: 361 DNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFYRANDKVAGSGLG 420 DNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFYRANDKVAGSGLG Sbjct: 361 DNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFYRANDKVAGSGLG 420 Query: 421 LAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPLAPPAA 459 LAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPLAPPAA Sbjct: 421 LAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPLAPPAA 459 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 211 bits (537), Expect = 9e-56, Method: Compositional matrix adjust. Identities = 157/469 (33%), Positives = 236/469 (50%), Gaps = 24/469 (5%) Query: 7 SIRRDLLKWLIAPLLLVNLIGAGLTYWLAWLPAQHAFDQNLMDSTWGLYAQVRHRQERTT 66 S+R L++WL+ PL L+ + A Y+ A A A+D++L+ ST L +V T Sbjct: 10 SLRSQLVRWLLIPLTLLVAVDAVSVYYNALEVADLAYDRSLLASTRALAERVSVVDGHVT 69 Query: 67 AELTQQAEQILRSNHSDTTFFAVRNSEGQIVVGDKGFPQLPA----SDVFDRPIN-YDGE 121 A++ A +++ ++ V G++V G P +P S+V+ + Y + Sbjct: 70 ADVPYVALDSFETDNLGRIYYKVTGLNGELVSGYDDLPPVPPDVKRSEVYPALVRFYQAD 129 Query: 122 MRGEPVRIAAMQVRVKN----GVIWVAVAETIRKRDRAHYTILLSFLVLDGALTFASIFV 177 +PVRIAA+ V + G+ + V ET+ R+ IL L G L + + Sbjct: 130 YHDQPVRIAALLQPVYDDSIRGIALIQVGETMEGRNGMTRKILFDTLWRQGTLVTVAALL 189 Query: 178 VLVAVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGEQAQ 237 V AVR LRPL L+ +E R L D E++PL+ AMN M R+ AQ Sbjct: 190 VWFAVRFVLRPLMQLKGEVESRAPTDLSGFDTALVHKEMRPLVQAMNGYMGRLQALISAQ 249 Query: 238 QNFMADVAHQLRTPLTGLKLQLEL-LHDKHQDQPDTA--RSLAM-MNSSVERMIRQSRQL 293 + F+AD +HQLRTPLT LK Q EL L + ++Q D A R L M + + + + +L Sbjct: 250 RRFIADASHQLRTPLTVLKTQSELTLRELRREQVDAAELRDLVEGMARTTDAAVHLANRL 309 Query: 294 LALARSEPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDLQ-PARLRGDRFLL 352 L LAR+E G E + LSL + ++A + I L F+ + + + LL Sbjct: 310 LTLARAEHGAAEGGTM-PLSLSDAARQVALELSQQAVSREIGLAFEEEGDVSIEANALLL 368 Query: 353 SDLIDNLIDNAVRYSPPHGTVTVRCLEEQD-----ATVLSVEDSGPGIAPEHRELIFDRF 407 +L+ NL+DNA+RY+P G VT+R + +L VEDSGPGI RE +F+ F Sbjct: 369 HELMVNLLDNAIRYTPAGGHVTLRVRAQSSPGQPTVALLEVEDSGPGIPAAEREKVFEPF 428 Query: 408 YRAND----KVAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRF 452 YRA G+GLGL+IVREIA H +++D QG G + + F Sbjct: 429 YRAGATQHVNPGGTGLGLSIVREIAAMHRASVTLDAPAQGSGLVVRIVF 477 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 185 bits (470), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 123/360 (34%), Positives = 192/360 (53%), Gaps = 26/360 (7%) Query: 122 MRGEPVRIAAMQVRVKNG-----------VIWVAVAETIRKRDRAHYTILLSFLVLDGAL 170 + G VRIA +QV ++ + V V ET+ KR + I+ ++ + Sbjct: 23 LHGSDVRIATLQVDLRRSAAQRAASREPRLATVQVGETLEKRAQLANEIIKGVILPQFII 82 Query: 171 TFASIFVVLVAVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARI 230 ++ +V A+ RGL PL LQ+ + R L ID P E+ PL+ ++N+++AR+ Sbjct: 83 LPVALALVWFALSRGLSPLSELQQRIRARRPDDLSPIDSGQVPEEISPLVRSLNDMLARL 142 Query: 231 NKGEQAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQS 290 ++ Q+ F+AD AHQ++TPL G+++Q EL + DQ D RSL + S E R Sbjct: 143 SQTIAIQKRFIADAAHQMKTPLAGMRMQSELAL-RQDDQVDVRRSLEQLAKSSETATRLV 201 Query: 291 RQLLALARSEPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDL--QPARLRGD 348 QLL+LAR+E +S + + L++L VQ ++ + +RIDLGF+ P + G+ Sbjct: 202 NQLLSLARAENQQSQSGGMIPVELNELARSVVQDWVPMSFTQRIDLGFEQPDHPILITGN 261 Query: 349 RFLLSDLIDNLIDNAVRYSP--PHGTVTVRCLEEQDA--TVLSVEDSGPGIAPEHRELIF 404 +L +L+ NL+DNA+ Y+P +G VTVR ++ +L VED+GPGI R +F Sbjct: 262 PVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGGGLAILEVEDNGPGIPVAERANVF 321 Query: 405 DRFYR---ANDKVAGSGLGLAIVREIAHDHGGVISVDCKEQGQ-----GTIFTVRFPLAP 456 +RFYR +G GLGLAIVREIA H + + + G +F V F + P Sbjct: 322 ERFYRILGTPSTGSGLGLGLAIVREIAQQHEAALEITSNPRSTDPKYPGCVFRVSFRMMP 381 >gi|300314014|ref|YP_003778106.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 171 bits (433), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 139/451 (30%), Positives = 213/451 (47%), Gaps = 17/451 (3%) Query: 7 SIRRDLLKWLIAPLLLVNLIGAGLTYWLAWLPAQHAFDQNLMDSTWGLYAQVRHRQERTT 66 S+R LL W+I+P+ L +I + Y A+D+ L+ S + +R Sbjct: 10 SVRAYLLAWIISPIALFIIIDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENGELK 69 Query: 67 AELTQQAEQILRSNHSDTTFFAVRNSEGQIVVGDKGFPQLPASD----VFDRPIN-YDGE 121 A L A +I +++S + + +G+ GD P ++ + Y+ Sbjct: 70 ASLPYAALEIYEADYSSRMIYRINGLDGKFFDGDADLPSYKGKADKEAIYPALAHIYEDT 129 Query: 122 MRGEPVRIAAM-QVRVKN---GVIWVAVAETIRKRDRAHYTILLSFLVLDGALTFASIFV 177 G PVR+AA+ Q V N G V VAET+ R I L+ AL + V Sbjct: 130 YNGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVIVSV 189 Query: 178 VLVAVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGEQAQ 237 L VRR L+P+ +L+R L++R L + AP ELQP I A+N+LMAR+ + Q Sbjct: 190 TLYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLLDHQ 249 Query: 238 QNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALA 297 Q F+A+ +HQLRTPL LK QL+ P + M+ +VER + QLL+LA Sbjct: 250 QRFVANASHQLRTPLAVLKTQLQSGLRGDAPAPVIWQE---MSGTVERATTLANQLLSLA 306 Query: 298 RSEPGLFESRKLEELS-LDKLVEESVQHFIEEADKKRIDLGFDLQPARLRGDRFLLSDLI 356 + E R +E+ L L E+ +K +D + + + G +++ +LI Sbjct: 307 KVEQ--IRGRGAQEVCDLGMLARETAVDLSPLIAEKDLDFELEAEKILVMGHPWMIGELI 364 Query: 357 DNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFYRANDKVAG 416 NL+ NA+R++P G + +R +D L + DSG GI + E +F F +N AG Sbjct: 365 SNLLHNAIRHTPAQGRLGIRITAREDVAELLIWDSGEGIDDQAMENVFKAF-SSNVSSAG 423 Query: 417 SGLGLAIVREIAHDHGGVISVDCKEQGQGTI 447 GLGL I EI IS+ + GTI Sbjct: 424 -GLGLTICGEIVDSMQATISLRNRVGEDGTI 453 >gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 459 Score = 169 bits (429), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 129/425 (30%), Positives = 211/425 (49%), Gaps = 25/425 (5%) Query: 44 DQNLMDSTWGLYAQVRHRQERTTAELTQQAEQILRSNHSDTTFFAVRNSEGQIVVGDKGF 103 D+ L+ S + QV + A ++ S D ++ V + ++ G F Sbjct: 41 DRALLASARMIAGQVGWNDGAPVVSVPPAALELFASPAQDAVYYQVLMDDDTLLAGRPDF 100 Query: 104 PQLPASDVFDRPINYDGEMRGEPVRIAA-MQVRVKNG---VIWVAVAETIRKR-----DR 154 P P P+ G+P+R+ + ++ G ++ V+V +T++ R D+ Sbjct: 101 PAAPDFAAL-YPVYSTTSFNGKPLRVVSYIRALFDEGTLRMVAVSVGQTMQGRQEMIDDQ 159 Query: 155 AHYTILLSFLVLDGALTFASIFVVLVAVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPV 214 H ++ ++L +I +V++ + LRPL +L+ L R L + G Sbjct: 160 WHASLRRQIVLL-----LLAISLVVLGLTMELRPLLALKDELATRRPDSLTPLRAGGLQQ 214 Query: 215 ELQPLIVAMNELMARINKGEQAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTAR 274 EL+P++ A+N+ + R+N Q+ F+AD AHQLRTPL + Q++ + D A+ Sbjct: 215 ELRPIVEAINQYIFRLNAQVTVQKRFVADAAHQLRTPLAVIDSQIQFAR-QLDDPARVAQ 273 Query: 275 SLAMMNSSVERMIRQSRQLLALARSE---PGLFESRKLEELSLDKLVEESVQHFIEEADK 331 LA + M + +LL L+++E F ++ L++ +V E + A K Sbjct: 274 VLASLQEGSRSMTDLTNKLLLLSQAEASDSSAFPRAPVDLLAVSAMVLEELSGL---ALK 330 Query: 332 KRIDLGFD--LQPARLRGDRFLLSDLIDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVE 389 K IDLG + L+ A + G+ L ++ NLIDNA+RY+P G VTV +++ +++V Sbjct: 331 KGIDLGLETTLESAMVNGNEAQLMGMLMNLIDNALRYTPEGGKVTVGLEKKRSQMIITVS 390 Query: 390 DSGPGIAPEHRELIFDRFYR-ANDKVAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIF 448 D+GPGI E RE +F+RFYR A GSGLGLAIVREI G I +D QG G + Sbjct: 391 DNGPGIPAEVRERVFERFYRNAAPDQPGSGLGLAIVREIVQASQGSIRLDAPAQGSGLVA 450 Query: 449 TVRFP 453 TV P Sbjct: 451 TVSLP 455 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,422,328 Number of extensions: 58826 Number of successful extensions: 334 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 246 Number of HSP's successfully gapped: 45 Length of query: 459 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 371 Effective length of database: 1,203,190 Effective search space: 446383490 Effective search space used: 446383490 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)