BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0016.p (473 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 895 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 254 1e-68 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 254 1e-68 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 241 7e-65 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 149 4e-37 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 895 bits (2312), Expect = 0.0, Method: Compositional matrix adjust. Identities = 468/477 (98%), Positives = 468/477 (98%), Gaps = 4/477 (0%) Query: 1 MSANQPPRLRDNPSSASAS----INDPVTQRALLWIVASGFFMQTLDTTIVNTALPAMAR 56 MSANQPPRLRD PSS S S INDPVTQRALLWIVASGFFMQTLDTTIVNTALPAMAR Sbjct: 22 MSANQPPRLRDTPSSPSTSASASINDPVTQRALLWIVASGFFMQTLDTTIVNTALPAMAR 81 Query: 57 SLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRMYFAAILLFSLGSLLCAMASSL 116 SLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRMYFAAILLFSLGSLLCAMASSL Sbjct: 82 SLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRMYFAAILLFSLGSLLCAMASSL 141 Query: 117 PMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASISIAGQLGPVVGPVLGGWLV 176 PMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASISIAGQLGPVVGPVLGGWLV Sbjct: 142 PMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASISIAGQLGPVVGPVLGGWLV 201 Query: 177 ESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRFDLLGCVLLSACMVAFSLALDVP 236 ESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRFDLLGCVLLSACMVAFSLALDVP Sbjct: 202 ESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRFDLLGCVLLSACMVAFSLALDVP 261 Query: 237 FDSHRTGWSITLFVVAALTVLGYWRHASVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSS 296 FDSHRTGWSI LFVVAALTVLGYWRHASVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSS Sbjct: 262 FDSHRTGWSIALFVVAALTVLGYWRHASVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSS 321 Query: 297 AVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVG 356 AVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVG Sbjct: 322 AVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVG 381 Query: 357 AAIMSFALITPDWPLTAAIVQLAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQ 416 AAIMSFALITPDWPLTAAIVQLAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQ Sbjct: 382 AAIMSFALITPDWPLTAAIVQLAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQ 441 Query: 417 MLAIGLGVTIGGTMVNLFKSEAGTATNASFQITFAIVGAITLASAWVFRKMDPRYFK 473 MLAIGLGVTIGGTMVNLFKSE GTATNASFQITFAIVGAITLASAWVFRKMDPRYFK Sbjct: 442 MLAIGLGVTIGGTMVNLFKSEVGTATNASFQITFAIVGAITLASAWVFRKMDPRYFK 498 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 254 bits (648), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 150/430 (34%), Positives = 236/430 (54%), Gaps = 5/430 (1%) Query: 42 LDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRMYFAAIL 101 +D+T+++T+LPA+A + +++ + +Y +++A P SGW+ADRFG+ ++ +AI Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 102 LFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASISIAG 161 +F LGS+LC ++S+LP +AAR +QG+GG+M++PVGRL +LR+V + AL+ ++I Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 162 QLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRFDLLGCVL 221 GPV+GP LGG++ W WIF IN+P+ +LG+ F+PD DL G L Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKG-FL 179 Query: 222 LSACMVAFSLALDVPFDSHRTGWSITLFVVAALTVL--GYWRHASVHPRPLFRLGLFGEP 279 LSA + + H + L V A V Y HA PL L L P Sbjct: 180 LSALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIP 239 Query: 280 NFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAKPWIAPLV 339 G+ G R+G A+PFLLPL+LQ+ GY+P H+GM+ +A K A + Sbjct: 240 TLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFL 299 Query: 340 KRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQFAAMNSVTLKG 399 +R+G+ R LLVN A+ + + A TP P +V L I G S+QF ++N+++ Sbjct: 300 RRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYAD 359 Query: 400 LDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEAGTAT--NASFQITFAIVGAIT 457 +DK S+ S+ Q L+IG+GV + G + + G AT F F +VGA+ Sbjct: 360 VDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVA 419 Query: 458 LASAWVFRKM 467 + + R++ Sbjct: 420 ACALLMLRQL 429 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 254 bits (648), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 164/471 (34%), Positives = 259/471 (54%), Gaps = 14/471 (2%) Query: 8 RLRDNPSSASASINDPVTQRALLWIVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHP 67 R +N ++ S+ D R L +IVA+ FFM+ LDTTI+ TALP MA+S G + Sbjct: 12 RGENNVATNDNSLTDARV-RMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSL 70 Query: 68 VVVAYTLTMASLTPASGWLADRFGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQG 127 + AY LT+A P SGW+ADRFG+R ++ AI +F+L S+LC + ++ AARV+QG Sbjct: 71 GMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQG 130 Query: 128 LGGSMLLPVGRLAVLRAVPGAQYIAALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLI 187 LGG+M++PVGR+ V+R+ + + A+A+I+ + PVVGP +GG++ SWHWIFL+ Sbjct: 131 LGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLM 190 Query: 188 NLPVGVLGMGAVLYFLPDDSLAGTPRFDLLGCVLLSACMVAFSLALDVPFDSH-RTGWSI 246 N+P G+ AV+ + ++ DL+G +L A + SL SH + + Sbjct: 191 NVPFGLAAFTAVMMVVRNEHGVSKRPLDLVGFLLSGAALT--SLLYGTEIASHMESNIKV 248 Query: 247 TLFVVAALTVLGY--WRHASVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPL 304 L VA VLGY WRH PL P +S+ + + RIG AVP+L+PL Sbjct: 249 ALGFVALGVVLGYGAWRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPL 308 Query: 305 LLQVRLGYSPLHAGMVLLPAAIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFAL 364 + Q+ G + +G++LL A+ K + +++RFG+ + N AI G I++ L Sbjct: 309 MFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGL 368 Query: 365 ITPDWPLTAAIVQLAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGV 424 +TP PL + + +G S SMQF+ + ++ + S ++L+S+ Q + IG+G+ Sbjct: 369 LTPATPLPVILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGI 428 Query: 425 TIGGTMVNLFKSEAGTATNAS--------FQITFAIVGAITLASAWVFRKM 467 G + + + G A +AS FQ FA+ G +TL S +R + Sbjct: 429 AFGALALRIAAALRGEAIDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGL 479 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 241 bits (616), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 143/432 (33%), Positives = 240/432 (55%), Gaps = 9/432 (2%) Query: 42 LDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRMYFAAIL 101 +D T++ T+LPA+AR L + + + + AY + P SGW+AD+ G R+++ AA++ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 102 LFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASISIAG 161 +F GS+LCA++ SL +AAR +QG+GG+M++PVGR+ + R+V ++ + A++ +++ Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 162 QLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRFDLLGCVL 221 LGPVVGP+LGG++ W W+FLIN+P LG+ F+ + D+ G VL Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 222 LS--ACMVAFSLALDVPFDSHRTGWS--ITLFVVAALTVLGYWRHASVHPRPLFRLGLFG 277 S A M+ L+L F SH + + + + A+ ++ Y+RH++ PL L Sbjct: 181 TSVGAAMIMLGLSL---FGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLR 237 Query: 278 EPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAKPWIAP 337 P + G + RIG AVPFLLPL LQ LG + AG + +A + Sbjct: 238 IPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGR 297 Query: 338 LVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQFAAMNSVTL 397 +++R+G+ L+VN G A+ ++ + + P + + + G ++QF +NS+ Sbjct: 298 VLRRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAY 357 Query: 398 KGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEAGTAT--NASFQITFAIVGA 455 +D RDAG SL S++Q L++GLGVT+ G ++ L G AT A F F ++G Sbjct: 358 ADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGL 417 Query: 456 ITLASAWVFRKM 467 ++AS + K+ Sbjct: 418 FSVASVPISGKL 429 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 149 bits (376), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 126/412 (30%), Positives = 195/412 (47%), Gaps = 21/412 (5%) Query: 33 VASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGT 92 V+ FM LD++I N A+P ++ +LG V V+ ++ A P +GWL R G Sbjct: 29 VSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGA 88 Query: 93 RRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIA 152 R++ ++LLF L S LC +A +LP+LLAARV+QGL ++P+ + +L A P ++ Sbjct: 89 VRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSF 148 Query: 153 ALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVL-YFLPDDSLAGT 211 ALA + + PV GP LGGW+ +S +W WIF IN+PVG++ G L + ++ Sbjct: 149 ALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHK 208 Query: 212 PRFDLLGCVLLSACMVAFSLALD--VPFDSHRTGWSITLFVVA--ALTVLGYWRHASVHP 267 D +G LL + AF + LD D + +TL ++A A W + H Sbjct: 209 LPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNNDH- 267 Query: 268 RPLFRLGLFGEPNFSIGLAGNLVCRIGSSAV---PFLLPLLLQVRLGYSPLHAGMVLLPA 324 P+ L LFG NF+ G + IG +LP LQ LGY + +G+ P Sbjct: 268 -PVVDLRLFGRRNFA---GGTIAISIGYGVFFGNLVILPQWLQQYLGYRSIDSGLSTAPI 323 Query: 325 AIAGTIAKPWIAPLVKRF--GYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIF- 381 I I + PL+ RF D + + +G AI+ + + + V L Sbjct: 324 GIFAVI----LMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLL 379 Query: 382 -GASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVN 432 G +M F + + L GL + L S V++ +G +I T N Sbjct: 380 QGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,426,942 Number of extensions: 58083 Number of successful extensions: 395 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 373 Number of HSP's successfully gapped: 20 Length of query: 473 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 384 Effective length of database: 1,198,455 Effective search space: 460206720 Effective search space used: 460206720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)