BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0006.p (345 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 696 0.0 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 166 3e-42 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 92 9e-20 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 78 1e-15 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 77 3e-15 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 696 bits (1797), Expect = 0.0, Method: Compositional matrix adjust. Identities = 343/345 (99%), Positives = 344/345 (99%) Query: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV Sbjct: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 Query: 61 LGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWATPPIHG 120 LGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWATPPIHG Sbjct: 61 LGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWATPPIHG 120 Query: 121 CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM 180 CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM Sbjct: 121 CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM 180 Query: 181 TALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRQALTDVVRQATDGWGADVVFE 240 TALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTR+ LTDVVRQATDGWGADVVFE Sbjct: 181 TALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRETLTDVVRQATDGWGADVVFE 240 Query: 241 ASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYANIFPRALA 300 ASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYANIFPRALA Sbjct: 241 ASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYANIFPRALA 300 Query: 301 LISSGMIDVKPFISRKFPFSQSIRAFEEAASGRPQDVKIQIEMEG 345 LISSGMIDVKPFISRKFPFSQSIRAFEEAASGRPQDVKIQIEMEG Sbjct: 301 LISSGMIDVKPFISRKFPFSQSIRAFEEAASGRPQDVKIQIEMEG 345 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 166 bits (419), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 113/362 (31%), Positives = 177/362 (48%), Gaps = 38/362 (10%) Query: 2 QALVLEATRELKLREIDL----PQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEA 57 Q LV+ A +L+++ +D PQQ+ ++++ GICGSDLHYY HG G +++ Sbjct: 3 QGLVIHAPHDLRIQALDTGALQPQQL-----KVKVKAGGICGSDLHYYHHGGFGVVRIKE 57 Query: 58 PMVLGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWA--- 114 PMVLGHE SG + EVG+AV+ + G R+ + P P G N +RF+ Sbjct: 58 PMVLGHEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAM 117 Query: 115 -TPPIHGCLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIG 173 TP + G ++ + + + D+VS E A+ EPLS+ L A +A G +V G Sbjct: 118 RTPHVQGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTG 177 Query: 174 AGTIGAMTALAALAGGAARVILADVV-----------AEKLAHFADNPAVITVDVTRQAL 222 G IGA+ +AA GA V++ DV A+ + A+ PA + Sbjct: 178 CGPIGALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKA------- 230 Query: 223 TDVVRQATDGWGADVVFEASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKE 282 A D DV+FEASG+ L+ + P V VG+ + + KE Sbjct: 231 -----YAADKGTFDVLFEASGNERALVGALEALRPQAIIVQVGL-GGDIQFPANLLVGKE 284 Query: 283 VRLESVFRYANIFPRALALISSGMIDVKPFISRKFPFSQSIRAFEEAASGRPQDVKIQIE 342 + FR+ F A+ L++ ++DVKP I+ +P +Q++ AF E A R + +K+QI Sbjct: 285 LEWRGAFRFHEEFAMAVELMNRKLVDVKPLITATYPIAQAVEAF-EIAGDRSRMMKVQIS 343 Query: 343 ME 344 + Sbjct: 344 FD 345 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 91.7 bits (226), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 107/357 (29%), Positives = 149/357 (41%), Gaps = 44/357 (12%) Query: 9 TRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMVLGHEASGT 68 ++ LK+ E+DL G +V +R+ G+C SDL T + P V PM LGHEAS Sbjct: 20 SQPLKIEELDL-DPPGPGEVLVRMKAAGLCHSDLSVIT--GVRPRPV--PMALGHEASAE 74 Query: 69 VIEVGSAVSHLKVGDRV-------------CMEPGIPRLDSP-ATLRGMYNLDPAVRFW- 113 V+EVGS V+ L+ GD V CME G P L P A + L R Sbjct: 75 VVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCME-GRPALCEPGAATNTVGELLSGQRRLR 133 Query: 114 -ATPPIHGCLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGL----------QAATKAR 162 T P+H L S A R A ++P+ L A A Sbjct: 134 DKTQPVHHHLGVSAFADHAVVSR---RSCVKIEADIDPVQAALFGCAVLTGVGAAVNTAE 190 Query: 163 MKPGDTAVVIGAGTIGAMTALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRQAL 222 +K G TA V+G G +G L ALA GA VI D+ KLA A TV+ Sbjct: 191 VKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDA 250 Query: 223 TDVVRQATDGWGADVVFEASGHAGVYQTLLDLVCPGGCAVLVGMPPAP---VALDVVAMQ 279 + VR T G G D FE +G + + GG V G+ PAP AL V++ Sbjct: 251 VEKVRALTRG-GVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGL-PAPGQHWALQQVSLV 308 Query: 280 TKEVRLESVFRYANI----FPRALALISSGMIDVKPFISRKFPFSQSIRAFEEAASG 332 +E ++ + + + PR + L +G + V + + + R F+ G Sbjct: 309 AEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFDRLHGG 365 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 82/291 (28%), Positives = 122/291 (41%), Gaps = 25/291 (8%) Query: 3 ALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMVLG 62 A+V E + L + ++ +P Q + ++ G+C +DLH HG P K P + G Sbjct: 8 AVVREFGKPLSIEQVPVPTPAPGQ-ILVKFEASGVCHTDLHA-AHGDW-PVKPTPPFIPG 64 Query: 63 HEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWATPPIHGCL 122 HE +G V VG+ V H+K GDRV G+P L + P W T Sbjct: 65 HEGTGYVAAVGAGVKHVKEGDRV----GVPWLHTACGC-----CSPCRTGWETLCAEQQN 115 Query: 123 TGSVVHPA---------AFTYRLPDNVSFAEGAIVEPLSIGLQAATK-ARMKPGDTAVVI 172 TG V+ + F LPDN+ F A V + + K ++PG+ V+ Sbjct: 116 TGYSVNGSFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVIS 175 Query: 173 GAGTIGAMTALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRQALTDVVRQATDG 232 G G +G M A A G V+ AD+ +KLA A +TVD V++ G Sbjct: 176 GIGGLGHMAVQYAKAMG-MHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGG 234 Query: 233 WGADVVFEASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEV 283 +V S A + + G LVG+PP ++L V K + Sbjct: 235 AHGALVTAVSPKA--MEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRI 283 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 76.6 bits (187), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 74/267 (27%), Positives = 116/267 (43%), Gaps = 27/267 (10%) Query: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 M+AL+ + ++++ + P A DV +++ ICGSDLH Y G I K A + Sbjct: 1 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLY-RGKIPATK--AGDI 57 Query: 61 LGHEASGTVIEVGSAVSHLKVGDRV-------------CMEPGIPRLDSPATLRGMYNLD 107 LGHE G V+E G A+ LK GDRV C + ++ RG Sbjct: 58 LGHEFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGALMNA 117 Query: 108 PAVRFWATPPIHGCLTGSVVHPAAFTYRLPD--------NVSFAEGAIV---EPLSIGLQ 156 +R A + L G + A R+P + + A+ ++ + L G Q Sbjct: 118 KGIRSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQ 177 Query: 157 AATKARMKPGDTAVVIGAGTIGAMTALAALAGGAARVILADVVAEKLAHFADNPAVITVD 216 A A ++ G + + GAG +G M A +A GA R+ + D A +L + VI V+ Sbjct: 178 AVKNAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPVN 237 Query: 217 VTRQALTDVVRQATDGWGADVVFEASG 243 + + + T G G D V +A G Sbjct: 238 FDQVDPAEYIIANTAGRGVDGVIDAVG 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,036,265 Number of extensions: 43348 Number of successful extensions: 185 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 5 Length of query: 345 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 259 Effective length of database: 1,212,660 Effective search space: 314078940 Effective search space used: 314078940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)