BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0126.p (540 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspiri... 965 0.0 gi|300312564|ref|YP_003776656.1| outer membrane multidrug resist... 281 1e-76 gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspiri... 245 6e-66 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 160 2e-40 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 151 1e-37 >gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 487 Score = 965 bits (2494), Expect = 0.0, Method: Compositional matrix adjust. Identities = 484/487 (99%), Positives = 486/487 (99%) Query: 54 MTRIAFSKLPVLTVIALSLTMAGCADQGGIQPQAQVRDVAVLDAGQAIRQAASEAGWPQR 113 MTRIAFSKLPVLTVIALSLTMAGCADQGGIQPQAQVRDVAVLDAGQAIRQAASEAGWPQR Sbjct: 1 MTRIAFSKLPVLTVIALSLTMAGCADQGGIQPQAQVRDVAVLDAGQAIRQAASEAGWPQR 60 Query: 114 AWWQAYGDAQLDQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKD 173 AWWQAYGDAQLDQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKD Sbjct: 61 AWWQAYGDAQLDQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKD 120 Query: 174 WSDNVYYGAAFRNKLTWNNTATLGLSYNVDLWDRNKNATLRAQDEMHAVAADARAAQLDL 233 WSDNVYYGAAFRNKLTWNNTATLGLSYNVDLWDRNKNATLRAQDEMHAVAADARAAQLDL Sbjct: 121 WSDNVYYGAAFRNKLTWNNTATLGLSYNVDLWDRNKNATLRAQDEMHAVAADARAAQLDL 180 Query: 234 EGNVVRTYVQLALQYGLLDNTEALLKEEQKILELAHRRFKGGLGTQLEITQAESPLPETR 293 EGNVVRTYVQLALQYGLLDNTEALLKEEQKIL+LAHRRFKGGLGTQLEITQAESPLPETR Sbjct: 181 EGNVVRTYVQLALQYGLLDNTEALLKEEQKILDLAHRRFKGGLGTQLEITQAESPLPETR 240 Query: 294 RQIEALHENIALIRNQLAALLGAGPGAADGITRPGMQLQTSLGLPSALPADLVGRRPDIS 353 RQIEALHENIALIRNQLAALLGAGPGAADG+TRPGMQLQTSLGLPSALPADLVGRRPDIS Sbjct: 241 RQIEALHENIALIRNQLAALLGAGPGAADGVTRPGMQLQTSLGLPSALPADLVGRRPDIS 300 Query: 354 AARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPI 413 AARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPI Sbjct: 301 AARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPI 360 Query: 414 FEGGRLRGQLGAASASYDIEVEHYNALLSQALKGISDQVVTLQSVARQQEQAQQSVQAAQ 473 FEGGRLRGQLGAASA YDIEVEHYNALLSQALKGISDQVVTLQSVARQQEQAQQSVQAAQ Sbjct: 361 FEGGRLRGQLGAASAGYDIEVEHYNALLSQALKGISDQVVTLQSVARQQEQAQQSVQAAQ 420 Query: 474 KNVDIATRAYQRGLTDYLNVLHAQTQWLRQQQIVQQLQAQRLAAYAALQTALGGGIEEAA 533 KNVDIATRAYQRGLTDYLNVLHAQTQWLRQQQIVQQLQAQRLAAYAALQTALGGGIEEAA Sbjct: 421 KNVDIATRAYQRGLTDYLNVLHAQTQWLRQQQIVQQLQAQRLAAYAALQTALGGGIEEAA 480 Query: 534 PAKVAAR 540 PAKVAAR Sbjct: 481 PAKVAAR 487 >gi|300312564|ref|YP_003776656.1| outer membrane multidrug resistance lipoprotein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 281 bits (719), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 184/471 (39%), Positives = 263/471 (55%), Gaps = 5/471 (1%) Query: 64 VLTVIALSLTMAGCADQGGIQPQAQVRDVAVLDAGQAIRQAASEAGWPQRAWWQAYGDAQ 123 VL + + MAGCA + +AQ + + + Q++ +A +A WP WW+ YGDAQ Sbjct: 28 VLAAVLAATLMAGCASFSDLGERAQPKSIDRYQSQQSLAASAVQAAWPSDQWWRVYGDAQ 87 Query: 124 LDQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKDWSDNVYYGAA 183 L+ L+ A+ PSMAVA AR+ +A+ A A P + S+ R S N Sbjct: 88 LNALIDEALQSAPSMAVAKARLMKAEGAAQQQGAALYPQVSANASLDRMKQSYNNGVPPD 147 Query: 184 FRNKLTWNNT--ATLGLSYNVDLWDRNKNATLRAQDEMHAVAADARAAQLDLEGNVVRTY 241 F K +NN ATL SY +D W +N+ A A E+ A ADA A++ L ++ Y Sbjct: 148 FVPK-DYNNATRATLDFSYEIDFWGKNRAALAAATSELEASRADAAQARITLATSIASAY 206 Query: 242 VQLALQYGLLDNTEALLKEEQKILELAHRRFKGGLGTQLEITQAESPLPETRRQIEALHE 301 +LA Y D EA LK + L+L ++RF GL T+ + Q E+ + ++A E Sbjct: 207 AELAQLYAQRDTNEAALKVRVESLDLFNQRFTNGLETRGSVKQMEARRAIAQADLKATDE 266 Query: 302 NIALIRNQLAALLGAGPGAADGITRPGMQLQTSLGLPSALPADLVGRRPDISAARWRVEA 361 +I L RN+LAALLGAGP +TRP + L LP+ LP +L+GRRPDI AAR R EA Sbjct: 267 SIGLQRNKLAALLGAGPDRGLQLTRPQIDLSRPFALPAQLPVELLGRRPDIVAARLRAEA 326 Query: 362 AAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPIFEGGRLRG 421 A K I VA+AAFYPN+NL A G + G + + A + + GPAISLPIF GGRLRG Sbjct: 327 AGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLT--RAGSDIGSIGPAISLPIFNGGRLRG 384 Query: 422 QLGAASASYDIEVEHYNALLSQALKGISDQVVTLQSVARQQEQAQQSVQAAQKNVDIATR 481 Q +ASASYD V +Y+ ++QAL+ ++D V+ +++A + Q + AA++ I + Sbjct: 385 QFRSASASYDEAVANYDQAVTQALQDVADVGVSEKALAGRLADVQAAADAAEEAYRIVSN 444 Query: 482 AYQRGLTDYLNVLHAQTQWLRQQQIVQQLQAQRLAAYAALQTALGGGIEEA 532 Y GL YL+VL+AQ + + + L+++ AL ALGGG A Sbjct: 445 RYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTLDVALVRALGGGYRAA 495 >gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 495 Score = 245 bits (626), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 169/465 (36%), Positives = 242/465 (52%), Gaps = 17/465 (3%) Query: 75 AGCADQGGIQPQAQVR---DVAVLDAGQAIRQAASEAGWPQRAWWQAYGDAQLDQLVTRA 131 +GCA GI QV D A + A WP W + +GDAQL LV +A Sbjct: 27 SGCASFAGIGSDRQVAQAGDFATQRSLSDPNPGAPNGQWPGSDWVRQFGDAQLVALVEQA 86 Query: 132 VAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKDWSDNVYYGAAFRNKLTWN 191 + +PS+ A AR+ A ++A A LPS + SV R +S Y + Sbjct: 87 LTSSPSLQQARARIAAASALAESRGAPLLPSVNAEASVTRNQFSSTTIYPPPYGGNWYNE 146 Query: 192 NTATLGLSYNVDLWDRNKNATLRAQDEMHAVAADARAAQLDLEGNVVRTYVQLALQYGLL 251 A L + Y +DLW++N+ A +A A A + A+L L ++V Y QLA QY L Sbjct: 147 KKAGLNVGYELDLWNKNQAALAQAISSEKAAQASEQEARLALTASIVTVYSQLAAQYALH 206 Query: 252 DNTEALLKEEQKILELAHRRFKGGLGTQLEITQAESPLPETRRQIEALHENIALIRNQLA 311 D ++ + + + ++ R + GL +Q+E Q+ + + R Q+ I L+R QL Sbjct: 207 DILQSTVDQRTSLEKITAERLRTGLDSQIERDQSRTSSADARAQLAQSEGQIVLLRQQLG 266 Query: 312 ALLGAGPGAADGITRPGMQLQTSLGLPSALPADLVGRRPDISAARWRVEAAAKGISVAKA 371 AL G GP + P +Q + GLP+ LP +L+GRRPDI AARW+VEAA++G+ VAKA Sbjct: 267 ALAGKGPDYGLQLAPPALQGLATPGLPAELPLNLMGRRPDIVAARWQVEAASRGVDVAKA 326 Query: 372 AFYPNINLLAGIGPAAAGGGLFSFL------SAPNLQTTYGPAISLPIFEGGRLRGQLGA 425 FYP+INL A IG F L +A + +GPAI+LPIFEGG LR L Sbjct: 327 RFYPDINLSAMIG--------FDTLLDSNPFTAASKSIAFGPAITLPIFEGGALRAGLKG 378 Query: 426 ASASYDIEVEHYNALLSQALKGISDQVVTLQSVARQQEQAQQSVQAAQKNVDIATRAYQR 485 ASY++ V YN L+ A ++ Q+ + + RQ +++QAA++ +A Y+ Sbjct: 379 EYASYELAVATYNKTLNDAYADVARQIAAIHATERQLPIRSEALQAAERAYALARERYRL 438 Query: 486 GLTDYLNVLHAQTQWLRQQQIVQQLQAQRLAAYAALQTALGGGIE 530 GL L +L AQT L Q+Q + LQAQR AL ALGGG + Sbjct: 439 GLVSQLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKALGGGFD 483 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 160 bits (406), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 141/448 (31%), Positives = 216/448 (48%), Gaps = 20/448 (4%) Query: 106 SEAGW---------PQRAWWQAYGDAQLDQLVTRAVAGNPSMAVAAARVRQAQSMAVVAH 156 SEA W P+ WW+ +GDAQL+ L +A+AGN ++A+A AR+ QA++ V Sbjct: 38 SEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTG 97 Query: 157 AGELPSAQLDVSVGRKDWSDN---VYYGAAFRNKLTWNNTATLG--LSYNVDLWDRNKNA 211 A + P L+ R S N Y + N T N LG +SY VD + R + + Sbjct: 98 AAQTPQVNLNARAARSRISANRPLTNYNSP--NFSTVQNDFALGASVSYEVDFFGRVQRS 155 Query: 212 TLRAQDEMHAVAADARAAQLDLEGNVVRTYVQLALQYGLLDNTEALLKEEQKILELAHRR 271 A AAD +L L + Y L LD + +++ LELA R Sbjct: 156 VEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVELDVVRRAIALQRQALELATAR 215 Query: 272 FKGGLGTQLEITQAESPLPETRRQIEALHENIALIRNQLAALLGAGPGAADGITRPGMQL 331 G + L++ Q ++ L T QI+ L A + +A L G P A IT P + Sbjct: 216 HDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIATLTGT-PAPAFSIT-PSLTP 273 Query: 332 QTSLGLPSALPADLVGRRPDISAARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAGGG 391 T +P +P+D++ RRPDI+AA + AA I VA AA+YP+ L G Sbjct: 274 ITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYPSFMLQPSYG--VDSRN 331 Query: 392 LFSFLSAPNLQTTYGPAISLPIFEGGRLRGQLGAASASYDIEVEHYNALLSQALKGISDQ 451 + +AP+L + G + S +F+GGRLR + + A Y+ V Y + A++ + D Sbjct: 332 WATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDG 391 Query: 452 VVTLQSVARQQEQAQQSVQAAQKNVDIATRAYQRGLTDYLNVLHAQTQWLRQQQIVQQLQ 511 + L ++ R Q+Q ++ +A++ ++IA+ Y+ G T YL+V+ AQ L ++ QL Sbjct: 392 ITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVITAQQSLLNSERQAAQLM 451 Query: 512 AQRLAAYAALQTALGGGIEEAAPAKVAA 539 QRL L ALGG + P AA Sbjct: 452 GQRLLVSVFLIKALGGDWQSRTPPAEAA 479 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 151 bits (382), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 135/437 (30%), Positives = 212/437 (48%), Gaps = 20/437 (4%) Query: 99 QAIRQAASEAGWPQRAWWQAYGDAQLDQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAG 158 +A Q A+EA P W + D L L+T+A+ + + AA RV++AQ++ + HA Sbjct: 50 EAAHQVANEAASPPLPWRDYFTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHAD 109 Query: 159 ELPS----AQLDVSVGRKDW--SDNVYYGAAFRNKLTWNNTATLGLS-YNVDLWDRNKNA 211 P+ A LD S D + G+A++ A LGLS + +DLW R ++ Sbjct: 110 LFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQ--------AGLGLSSWEIDLWGRLRSL 161 Query: 212 TLRAQDEMHAVAADARAAQLDLEGNVVRTYVQLALQYGLLDNTEALLKEEQKILELAHRR 271 A + A A A L L V +++LA L+ + Q+ + RR Sbjct: 162 DDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQESFRIFSRR 221 Query: 272 FKGGLGTQLEITQAESPLPETRRQIEALHENIALIRNQLAALLGAGPGAADGITRPGMQL 331 + G ++L +TQ E+ L + + L + A N L L+G+ G R G+ Sbjct: 222 VEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLVGSPVVLPVGQARAGLDA 281 Query: 332 QTSLGLPSALPADLVGRRPDISAARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAG-G 390 + L LPA L+ RRPD++AA R+ AA I A+AAF+P+I+L +G A+A Sbjct: 282 VFA-PLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELS 340 Query: 391 GLFSFLSAPNLQTTYGPAISLPIFEGGRLRGQLGAASASYDIEVEHYNALLSQALKGISD 450 GLF S T+ P+I+LPIF GRLR L + D+ V Y + A + ++D Sbjct: 341 GLFRDGSH---AWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVAD 397 Query: 451 QVVTLQSVARQQEQAQQSVQAAQKNVDIATRAYQRGLTDYLNVLHAQTQWLRQQQIVQQL 510 + Q + +Q E AQ +V+ ++ ++ Y G +L+VL AQ L QQ + Q+ Sbjct: 398 ALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQI 457 Query: 511 QAQRLAAYAALQTALGG 527 + L++ AL ALGG Sbjct: 458 RRALLSSSVALYAALGG 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,316,969 Number of extensions: 43916 Number of successful extensions: 273 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 231 Number of HSP's successfully gapped: 19 Length of query: 540 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 450 Effective length of database: 1,193,720 Effective search space: 537174000 Effective search space used: 537174000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)