BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0123.p (324 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pum... 649 0.0 gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 92 4e-20 gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 80 3e-16 gi|300311362|ref|YP_003775454.1| HlyD family secretion, possible... 62 9e-11 >gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 649 bits (1675), Expect = 0.0, Method: Compositional matrix adjust. Identities = 322/324 (99%), Positives = 322/324 (99%) Query: 1 MSVKSIFRVLITTAIFLLAILLAWSLWQHYMHSPWTRDGRVRAEVVNIAPDVSGQVVALP 60 MSVKSIFRVLITTAIFLLAILLAWSLWQHYMHSPWTRDGRVRAEVVNIAPDVSGQVVALP Sbjct: 1 MSVKSIFRVLITTAIFLLAILLAWSLWQHYMHSPWTRDGRVRAEVVNIAPDVSGQVVALP 60 Query: 61 VRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKAELDVRRAEVEARRADLNMRRAQA 120 VRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKAELDVRRAEVEARRADLNMRRAQA Sbjct: 61 VRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKAELDVRRAEVEARRADLNMRRAQA 120 Query: 121 RRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQYQAALSQRDTARLNLER 180 RRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQYQAALSQRDTARLNLER Sbjct: 121 RRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQYQAALSQRDTARLNLER 180 Query: 181 TKVFSPVDGYITNLNVRRGDYAATGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDRARIQ 240 TKVFSPVDGYITNLNVRRGDYAA GAAKIAVIDSHSFWVYAYFEETKLPLLSIGDRARIQ Sbjct: 181 TKVFSPVDGYITNLNVRRGDYAAAGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDRARIQ 240 Query: 241 LINGASMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIQLDKVPENVL 300 LINGASMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIQLDKVPENVL Sbjct: 241 LINGASMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIQLDKVPENVL 300 Query: 301 LASGLTCTVVLEPGSGKQSAVAKN 324 LASGLTCTVVLEPGSGKQ AVAKN Sbjct: 301 LASGLTCTVVLEPGSGKQPAVAKN 324 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 92.4 bits (228), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 99/320 (30%), Positives = 153/320 (47%), Gaps = 56/320 (17%) Query: 44 EVVNIAPDVSGQVVALPVRDNQLVKKGDLLMEIDPARYTLALQQAEAAV--TARKAE--- 98 E+ + P ++G V A+ V D Q VKKGD+L+ ID A LA+ QA A + T RK + Sbjct: 72 EIATVTPAINGIVAAVDVVDTQAVKKGDVLVRIDDADARLAVDQAAADLDRTERKVKGFF 131 Query: 99 -------LDVRRAEVEARRADLNMRRAQAR-RRADVD-----ALV----VSREAREDAGS 141 V E E +RA + AQA +RA++D AL VS E +A + Sbjct: 132 ANDAGLAAQVLAREAEQKRASAQLLSAQADLKRAEIDLQRREALAKSGSVSGEELSNART 191 Query: 142 QVTASQAQLEAAQA----------QLQGAQA---------------QYQAALSQRDTARL 176 + +QA L+AA+A QGAQ + A ++ + A+L Sbjct: 192 ALLTAQANLKAAEAAEVQSRANIKATQGAQKASTVLTANTTVDDNPEVVLARAKLEQAKL 251 Query: 177 NLERTKVFSPVDGYITNLNVRRGDYAATGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDR 236 +LERT + +PVDG I V+ G +GA ++V+ V A F+E +L + IG Sbjct: 252 DLERTVLRAPVDGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLTKVRIGQP 311 Query: 237 ARIQL-INGASMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIQLDKV 295 ++ + G S+E H + G+ + A N T NW+++ QR+PVRI LD Sbjct: 312 VTMKADLYGGSVEYH--GVVTGLSGGTGSAFAVIPAQ-NATGNWIKVVQRLPVRISLD-- 366 Query: 296 PENVL---LASGLTCTVVLE 312 P+ + L+ GL+ V ++ Sbjct: 367 PKELAQRPLSVGLSMVVEID 386 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 79.7 bits (195), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 103/329 (31%), Positives = 149/329 (45%), Gaps = 41/329 (12%) Query: 18 LAILLAWSLWQHYMHSPWTRDGRVRAEVVNIAPDVSGQVVALPVRDNQLVKKGDLLMEID 77 +A L W L + T D V VV I+ V G VVA+ D Q+VK G L+ +D Sbjct: 36 VACFLYWFLHARFFEE--TDDAYVGGNVVQISAQVGGTVVAVKADDTQVVKAGQQLVALD 93 Query: 78 PA--RYTLALQQAEAAVTARKA-----ELDVRRAEVEARRADLNMRRAQA---RRRADVD 127 A R L QA A R+ D A V A AD N+ RA+ RR+A + Sbjct: 94 AADTRLALDQAQAALAQAVRQTRQLFLNNDTLAANVAA--ADSNLARAREDLQRRQAGLS 151 Query: 128 ALVVSRE----AREDAGSQVTASQAQLEAAQAQLQGAQAQ--------YQAALSQRDTAR 175 + VS+E AR+ S V A AA A QAA + R+ A Sbjct: 152 SGAVSQEDVSHARDAVKSAVAALDQARAAAAANRALTDHTSVTEHPNVLQAATAVRN-AY 210 Query: 176 LNLERTKVFSPVDGYITNLNVRRGDYAATGAAKIAVIDSHSFWVYAYFEETKLPLLSIGD 235 LN R + +PV G+++ +V+ G A G +A++ W+ A F+E++L + IG Sbjct: 211 LNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRIGQ 270 Query: 236 RAR-IQLINGASM--EGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIQL 292 I + G+S+ +G V + G + L N T NW+++ QR+PVRI L Sbjct: 271 PVEVIADVYGSSVKYKGTVIGFSAG-----TGGAFSLLPAQNATGNWIKVVQRVPVRIAL 325 Query: 293 DKVPENV---LLASGLTCTVVLE-PGSGK 317 D PE V L GL+ T ++ G G+ Sbjct: 326 D--PEQVRAHPLRIGLSTTATVDIHGDGR 352 >gi|300311362|ref|YP_003775454.1| HlyD family secretion, possible efflux component [Herbaspirillum seropedicae SmR1] Length = 407 Score = 61.6 bits (148), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 54/162 (33%), Positives = 84/162 (51%), Gaps = 14/162 (8%) Query: 39 GRVRA-EVVNIAPDVSGQVVALPVRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKA 97 G V+A + V++ VSGQ+ +L V VKKG L+ EID AL AE ++ +A Sbjct: 58 GTVKAYKQVSVGAQVSGQIKSLKVALGDQVKKGQLVAEIDSLTQANALASAEFSLQNLQA 117 Query: 98 ELDVRRAEVEARRADLNMRRAQARRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQL 157 +L R E ++A L A AR++ + SRE E A + + +QA + A QAQ+ Sbjct: 118 QL--RAKEASLKQAQL----AYARQKMMLAGDASSRENFESAEATLNTTQADIAALQAQI 171 Query: 158 QGAQAQYQAALSQRDTARLNLERTKVFSPVDGYITNLNVRRG 199 + + DTARLNL T++ SP++G + + + G Sbjct: 172 KDGAIKV-------DTARLNLGYTRISSPIEGQVVAIVAQEG 206 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 745,250 Number of extensions: 25484 Number of successful extensions: 130 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 12 Length of query: 324 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 239 Effective length of database: 1,217,395 Effective search space: 290957405 Effective search space used: 290957405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)