BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0104.p (382 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 796 0.0 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 109 5e-25 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 96 6e-21 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 91 1e-19 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 88 1e-18 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 796 bits (2055), Expect = 0.0, Method: Compositional matrix adjust. Identities = 382/382 (100%), Positives = 382/382 (100%) Query: 1 MEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVMHYTQQ 60 MEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVMHYTQQ Sbjct: 28 MEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVMHYTQQ 87 Query: 61 KKIEGVTIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYV 120 KKIEGVTIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYV Sbjct: 88 KKIEGVTIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYV 147 Query: 121 CDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIILAD 180 CDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIILAD Sbjct: 148 CDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIILAD 207 Query: 181 EIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHARDYIEG 240 EIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHARDYIEG Sbjct: 208 EIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHARDYIEG 267 Query: 241 LNMLASMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRDLAHQLLTDIPGVSCVK 300 LNMLASMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRDLAHQLLTDIPGVSCVK Sbjct: 268 LNMLASMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRDLAHQLLTDIPGVSCVK 327 Query: 301 PKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGTGFNCPTPDHFRVVFLPNTDD 360 PKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGTGFNCPTPDHFRVVFLPNTDD Sbjct: 328 PKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGTGFNCPTPDHFRVVFLPNTDD 387 Query: 361 LTESMGRIAHFLEGYRRRHGTA 382 LTESMGRIAHFLEGYRRRHGTA Sbjct: 388 LTESMGRIAHFLEGYRRRHGTA 409 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 109 bits (272), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 92/369 (24%), Positives = 173/369 (46%), Gaps = 18/369 (4%) Query: 2 EEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR--NMGNASGYTDSKGLFAPRKSVMH-YT 58 G II +++GN P IV+ ++ + GY+ SKG+ R+++ H Y Sbjct: 63 RRRGEDIIDMSMGNPDG---ATPPHIVEKLVEVAQRPDTHGYSSSKGIPRLRRAIAHWYR 119 Query: 59 QQKKIEGVTIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVH 118 + +++ + + G+ E + + A L+ GD VLVP+P YP+ ++G Sbjct: 120 SRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSYPIHIYGAVIAGANIRS 179 Query: 119 YVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIIL 178 + ++ ++ P K +++ P+NPT +E + +++LAR+HQ++++ Sbjct: 180 VRMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTAQCVELEFFERVVKLAREHQILVV 239 Query: 179 ADEIYDKVLYDGNTHTSLASL--ADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHARD 236 D Y + +DG S+ + A +V + F LSK+Y G+R G+MV + A Sbjct: 240 HDLAYADITFDGWKAPSIMQVPGAREVA-VEFFTLSKSYNMAGWRIGFMVGNARLVAALA 298 Query: 237 YIEGLNMLASMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRDLAHQLLTDIPGV 296 I+ + S P Q A AL G QS + + + ++R++ + L + G Sbjct: 299 RIKSYHDYGSF-----TPVQVAAIAALEGDQSCVEEI--RANYERRRNVLVKGLHEA-GW 350 Query: 297 SCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGTGFNCPTPDHFRVVFLP 356 PK+++Y++ R+ PE Y EFA LL + KV + G GF ++ R + Sbjct: 351 MVDVPKASMYIWARI-PEPYRQFGSLEFARILLEQAKVCVSPGIGFGEYGDEYVRFALIE 409 Query: 357 NTDDLTESM 365 N + +++ Sbjct: 410 NESRIRQAI 418 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 95.5 bits (236), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 97/382 (25%), Positives = 175/382 (45%), Gaps = 23/382 (6%) Query: 1 MEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNAS-GYTDSKGLFAPRKSVM-HYT 58 +E++G +I + IG F AP +V+ R M YT + GL R ++ HY Sbjct: 28 LEQQGRHLIHMGIGEP---DFTAPPAVVEAATRAMTEGKMQYTSATGLPQLRAAISDHYR 84 Query: 59 QQKKIEGVTIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVH 118 +E + + I + GAS ++++ AL+ EVL+P P YP V+ G Sbjct: 85 SVYGLE-IAPERIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFEGRAKL 143 Query: 119 YVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIIL 178 + +Q +++ T+ +++ +P+NPTG + L+ I+ RQ + Sbjct: 144 IASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTGTSILPDELRAIVGEVRQRGGFTI 203 Query: 179 ADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHARDYI 238 DEIY + Y+G ++L SL DDV+ I N SK + G+R GW+V+ + Sbjct: 204 VDEIYQGLSYEGAPFSAL-SLGDDVVVI--NSFSKYFNMTGWRLGWLVLPPA------LV 254 Query: 239 EGLNMLA-SMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRDLAHQLLTDIPGVS 297 + LA ++ +CA++ Q A A Q++ + ++RD L + Sbjct: 255 PQVEKLAQNLLICASSIAQHA-AVACFTPQTLALYEERKAEFKRRRDYIVPALESLGFTV 313 Query: 298 CVKPKSALYMFPRLDPEIYPIKDDQ-EFAYELLAEEKVLIVQGTGFNCPTPDHF-RVVFL 355 V P A Y++ + + DD + + ++L E V++V G F T + R+ + Sbjct: 314 PVMPDGAFYVY----ADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTARRYIRLSYA 369 Query: 356 PNTDDLTESMGRIAHFLEGYRR 377 + ++L E++ R+ F E R+ Sbjct: 370 TSMENLQEAVARLRVFFEKRRK 391 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 91.3 bits (225), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 53/162 (32%), Positives = 92/162 (56%), Gaps = 6/162 (3%) Query: 68 IDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYVCDE--QQ 125 + +I + GA++ I+ SV ++ GDEV+V P Y + A+ L+GG PV +V E Q Sbjct: 89 VSEITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPV-FVQMEVGAQ 147 Query: 126 GWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIILADEIYDK 185 G+ +K +TP T+ I+V P+NPTG++ + + ++ R ++IL+DE+Y+ Sbjct: 148 GYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYEH 207 Query: 186 VLYDGNTHTSLASLAD--DVLFITFNGLSKNYRSCGYRAGWM 225 ++YDG H SLA + + FI + K Y G++ G++ Sbjct: 208 MVYDGQPHESLARHPELAERSFIN-SSFGKTYHVTGWKVGYV 248 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 88/389 (22%), Positives = 172/389 (44%), Gaps = 39/389 (10%) Query: 3 EEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVMHYTQQK- 61 + ++ I L IG P I Q +I N+ + Y + G A R ++ + Q++ Sbjct: 43 DPAYRPISLGIGEPK---HPTPAFIQQALIDNLAGLASYPATLGSDALRGAIADWLQKRY 99 Query: 62 ---KIEGVTIDDIYLGNGASELIVMSVNALLNT---GDEVLVPSPDYPLWTAAVSLSGGT 115 + + T +I NG+ E + +++ G V+ P+P Y ++ + L+G Sbjct: 100 DIPRPDPAT--EILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQ 157 Query: 116 PVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQL 175 P D ++ + PD + + + + + + V +P NPTGA+ ++E KE+ L+ ++ Sbjct: 158 PYFVNSDPKRNFAPDFSQVPQDVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGF 217 Query: 176 IILADEIYDKVLYD-----GNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGE 230 +I +DE Y ++ ++ G + D I+F+ LSK G R+G+ V+G+ Sbjct: 218 VIASDECYSEIYFNEDPPLGGMQAARLLGRDYRRLISFSSLSKRSNVPGMRSGF--VAGD 275 Query: 231 KKHARDYIEGLNMLASMRLCANAPGQFAIQTA-LGGYQSINDLVGPQGRLTKQRDLAHQL 289 + ++ + + A +P IQ A + +Q + G + + L Sbjct: 276 AAILKKFL----LYRTYHGAAMSP---VIQAASVLAWQDETHVQGNIAKYVTKFSQVTPL 328 Query: 290 LTDIPGVSCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGT-------GF 342 L ++ V+ P +A Y++ ++D + D EFA L AE V ++ G+ G Sbjct: 329 LQEVLEVAL--PDAAFYLWAKVDKLVN--ISDTEFAQRLYAEYNVTVLPGSYLAREAHGV 384 Query: 343 NCPTPDHFRVVFLPNTDDLTESMGRIAHF 371 N P R+ + ++ E+ RI F Sbjct: 385 N-PGAGRIRMALVAEVEECLEAAQRIVAF 412 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,236,159 Number of extensions: 51026 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 6 Length of query: 382 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 295 Effective length of database: 1,207,925 Effective search space: 356337875 Effective search space used: 356337875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)