BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0056.p (465 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipo... 905 0.0 gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [... 195 6e-51 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 160 1e-40 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 158 8e-40 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 156 3e-39 >gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 470 Score = 905 bits (2339), Expect = 0.0, Method: Compositional matrix adjust. Identities = 460/464 (99%), Positives = 461/464 (99%) Query: 1 MSLPVPLRLSLLLALTLLGACSPLTRSAYTPVSTQTPASWQQRGNQPEADVQRWWQGFGD 60 MSLPVP RLSLLLALTLLGACSPLTRSAYTPVSTQTPASWQQRG+QPEADVQRWWQGFGD Sbjct: 6 MSLPVPFRLSLLLALTLLGACSPLTRSAYTPVSTQTPASWQQRGSQPEADVQRWWQGFGD 65 Query: 61 ATLTALIDEALRKNNDLALAAIKLRRAQAQAGLADDAFVPALSASLGTSSSKQLDGDRIS 120 ATLTALIDEALRKNNDLALAAIKLRRAQAQAGLADDAFVPALSASLG SSSKQLDGDRIS Sbjct: 66 ATLTALIDEALRKNNDLALAAIKLRRAQAQAGLADDAFVPALSASLGASSSKQLDGDRIS 125 Query: 121 TRASQSSVKLSYEVDLWGRLAAASDLSRWEALATEQDRQSTALTLIGTTANLYWTGAYLN 180 TRASQSSVKLSYEVDLWGRLAAASDLSRWEALATEQDRQSTALTLIGTTANLYWTGAYLN Sbjct: 126 TRASQSSVKLSYEVDLWGRLAAASDLSRWEALATEQDRQSTALTLIGTTANLYWTGAYLN 185 Query: 181 QRVATAEQSIAYAEKTLELVRTQYQAGAVSSVEVLSAQQNLYSQQSSYTELVQQRVENDN 240 QRVATAEQSIAYAEKTLELVRTQYQAGAVSSVEVLSAQQNLYSQQSSYTELVQQRVENDN Sbjct: 186 QRVATAEQSIAYAEKTLELVRTQYQAGAVSSVEVLSAQQNLYSQQSSYTELVQQRVENDN 245 Query: 241 ALAILFDQPPGKAIALPARLPEQALPVLAAGVPAELLGRRPDLRALELRIRSALAGVDQA 300 ALAILFDQPPGKAIALPARLPEQALPVLAAGVPAELLGRRPDLRALELRIRSALAGVDQA Sbjct: 246 ALAILFDQPPGKAIALPARLPEQALPVLAAGVPAELLGRRPDLRALELRIRSALAGVDQA 305 Query: 301 RLNYYPQLSLTGTLGTSSDALLRLVQNPVATLGGALTLPFLQHSEMKFKVGISQADFDTA 360 RLNYYPQLSLTGTLGTSSDALLRLVQNPVATLGGALTLPFLQHSEMKFKVGISQADFDTA Sbjct: 306 RLNYYPQLSLTGTLGTSSDALLRLVQNPVATLGGALTLPFLQHSEMKFKVGISQADFDTA 365 Query: 361 VLNFRKGLYTAFSEVENALSARQQYARQQALLVRNLDAAQAAERLLEVRYRAGSATLQLW 420 VLNFRKGLYTAFSEVENALSARQQYARQQALLVRNLDAAQAAERLLEVRYRAGSATLQLW Sbjct: 366 VLNFRKGLYTAFSEVENALSARQQYARQQALLVRNLDAAQAAERLLEVRYRAGSATLQLW 425 Query: 421 LDQQDKRRTAEISLAQVRLNQLKNQMTLYQALGGDASVPAAISA 464 LDQQDKRRTAEISLAQVRLNQLKNQMTLYQALGGDASVPAAI A Sbjct: 426 LDQQDKRRTAEISLAQVRLNQLKNQMTLYQALGGDASVPAAIIA 469 >gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [Herbaspirillum seropedicae SmR1] Length = 492 Score = 195 bits (496), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 144/456 (31%), Positives = 231/456 (50%), Gaps = 18/456 (3%) Query: 19 GACSPLTRSAYTPVSTQTPASWQQRGNQPEADVQR---------WWQGFGDATLTALIDE 69 G C+ + Q P W G ADV WW+ FG L+ L+++ Sbjct: 33 GGCAMREVQTDPILEMQVPDKW---GGGASADVAHAEQLWPDTDWWKQFGSNELSELVEQ 89 Query: 70 ALRKNNDLALAAIKLRRAQAQAGLADDAFVPALSASLGTSSSKQLDGDRISTRASQSSVK 129 R N ++A A ++++A+AQA +A +P+ + G + + G T + +S++ Sbjct: 90 GQRNNYEIAAAYSRVKQAEAQARIAGAPLLPSAGINAGAAREIPISGG-TPTNTANASLQ 148 Query: 130 LSYEVDLWGRLAAASDLSRWEALATEQDRQSTALTLIGTTANLYWTGAYLNQRVATAEQS 189 +SYE+D WG+ A + + A DR++ ALT+ + Y L R+A A Q+ Sbjct: 149 VSYEIDFWGKNRAGVEAAEATLRANRYDRETVALTVTSGIVSTYLQVLSLRDRLAIARQN 208 Query: 190 IAYAEKTLELVRTQYQAGAVSSVEVLSAQQNLYSQQSSYTELVQQRVENDNALAILFDQP 249 + AE+ L LV Q +AG+ S +++ + L +Q+ +L QQ + ALAIL +P Sbjct: 209 VQNAERVLRLVEAQSRAGSASPLDLARQRSALANQRQLIPDLQQQEQDAQTALAILLGRP 268 Query: 250 PGKAIALPARLPEQALPVLAAGVPAELLGRRPDLRALELRIRSALAGVDQARLNYYPQLS 309 P L +P ++AG+P+ELL RRPD+R E + +A A V AR +P ++ Sbjct: 269 PQNFKVEEKGLARIQMPRISAGLPSELLTRRPDIRRAEASLAAANANVAVARAALFPSIT 328 Query: 310 LTGTLGTSSDALLRLVQNP--VATLGGALTLPFLQHSEMKFKVGISQADFDTAVLNFRKG 367 LTG+ G S ALL L P A LG L P +++ + ++ A + V +R+ Sbjct: 329 LTGSTGAQSSALLSLFDGPNLFANLGAGLIAPIFDGGKLRGQRDLAIAQKEELVQVYRQR 388 Query: 368 LYTAFSEVENALSARQQYARQQALLVRNLDAAQAAERLLEVRYRAGSATLQLWLDQQDKR 427 + T+ SEVE AL A + + L V +D A+ A L E+RYRAG+ L + LD Q Sbjct: 389 VITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLLVVLDTQRTL 448 Query: 428 RTAEISLAQVRLNQLKNQMTLYQALGG---DASVPA 460 A+ L Q++L +L+ ++LY+ALGG D+ P Sbjct: 449 SEAQNQLGQIKLQRLQATVSLYKALGGGWQDSQAPG 484 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 160 bits (406), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 138/474 (29%), Positives = 241/474 (50%), Gaps = 27/474 (5%) Query: 1 MSLP-VPLRLSLLLALTL---LGACS--PLTRSAYTPVSTQTPASWQQRGNQPEADVQRW 54 +S+P P L + AL + LG CS P PV + PA R ++ W Sbjct: 4 LSMPRSPRLLHMCAALVMAGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNLG-W 62 Query: 55 WQGFGDATLTALIDEALRKNNDLALAAIKLRRAQAQAGLADDAFVPALS-----ASLGTS 109 Q F D L LI AL N DL A + + A+AQ +A +P L+ A T+ Sbjct: 63 RQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTA 122 Query: 110 SSKQLDGDRISTRASQSSVKL-SYEVDLWGRLAAASDLSRWEALATEQDRQSTALTLIGT 168 +S+ L G + + Q + + +YE+D +GR+ D + L+TE+ ++S ++L+ Sbjct: 123 ASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQ 182 Query: 169 TANLYWTGAYLNQRVATAEQSIAYAEKTLELVRTQYQAGAVSSVEVLSAQQNLYSQQSSY 228 A Y +++ A++++ + L+L + + GA ++++ Q + S + + Sbjct: 183 VAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAK 242 Query: 229 TELVQQRVENDNALAILFDQPPGKAIALPARL--PEQALPVLAAGVPAELLGRRPDLRAL 286 T+L +QR + +NAL +L + G+ PA+L E+ + + AG+P++LL RPD+R+ Sbjct: 243 TQLERQRAQAENALTLLVGKKIGELP--PAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSA 300 Query: 287 ELRIRSALAGVDQARLNYYPQLSLTGTLGTSSDALLRLVQNPVA--TLGGALTLPFLQHS 344 E + +A A + AR ++P++SLTG G++S L L ++ + G +L+LP Sbjct: 301 EQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFG 360 Query: 345 EMKFKVGISQADFDTAVLNFRKGLYTAFSEVENALSAR----QQYARQQALLVRNLDAAQ 400 + +++ + AV+ + K + TAF EV +AL AR +Q Q+A L A Q Sbjct: 361 RNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLA----AQQ 416 Query: 401 AAERLLEVRYRAGSATLQLWLDQQDKRRTAEISLAQVRLNQLKNQMTLYQALGG 454 +L ++R++ G A+ LD Q +A+ +L Q R +L N + LY+ALGG Sbjct: 417 ERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGG 470 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 158 bits (400), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 134/427 (31%), Positives = 214/427 (50%), Gaps = 16/427 (3%) Query: 41 QQRGNQPEADVQRWWQGFGDATLTALIDEALRKNNDLALAAIKLRRAQAQAGLADDAFVP 100 Q N+ + W F D L ALI +AL + DL AA++++ AQA G+ P Sbjct: 53 HQVANEAASPPLPWRDYFTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHADLFP 112 Query: 101 ALSASLGTSSSKQLDGDRISTR-----ASQSSVKLS-YEVDLWGRLAAASDLSRWEALAT 154 + A G S+ ++ R A Q+ + LS +E+DLWGRL + D + A A+ Sbjct: 113 TIGAQAGLDRSRTPADLNLTRRPLVGSAYQAGLGLSSWEIDLWGRLRSLDDAALESARAS 172 Query: 155 EQDRQSTALTLIGTTANLYWTGAYLNQRVATAEQSIAYAEKTLELVRTQYQAGAVSSVEV 214 + R + AL+L+ A+ + A L++R+ A Q+IA +++ + + + GA S + + Sbjct: 173 QATRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQESFRIFSRRVEVGATSRLNL 232 Query: 215 LSAQQNLYSQQSSYTELVQQRVENDNALAILFDQPPGKAIALPARLPEQALPVLAA---- 270 + L Q+ +L QQR + NAL +L P + LP L + A Sbjct: 233 TQIETLLTQAQALGAQLQQQRAQRLNALTLLVGSP----VVLPVGQARAGLDAVFAPLQP 288 Query: 271 GVPAELLGRRPDLRALELRIRSALAGVDQARLNYYPQLSLTGTLGTSSDALLRLVQNPVA 330 G+PA LL RRPD+ A E R+ +A A + AR ++P +SLTG +GT+S L L ++ Sbjct: 289 GLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELSGLFRDGSH 348 Query: 331 --TLGGALTLPFLQHSEMKFKVGISQADFDTAVLNFRKGLYTAFSEVENALSARQQYARQ 388 T ++TLP ++ + +S+ D AV + K + TAF EV +AL+ARQ +Q Sbjct: 349 AWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADALAARQWLTQQ 408 Query: 389 QALLVRNLDAAQAAERLLEVRYRAGSATLQLWLDQQDKRRTAEISLAQVRLNQLKNQMTL 448 + + + RL +RY G+A LD Q +A+ L Q+R L + + L Sbjct: 409 LEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRALLSSSVAL 468 Query: 449 YQALGGD 455 Y ALGG+ Sbjct: 469 YAALGGE 475 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 156 bits (395), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 139/451 (30%), Positives = 223/451 (49%), Gaps = 28/451 (6%) Query: 29 YTPVSTQTPASW-QQRGNQP------------EADVQRWWQGFGDATLTALIDEALRKNN 75 YT + PASW Q QP +AD W + F D L +I AL N Sbjct: 35 YTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVIAAALDNNR 94 Query: 76 DLALAAIKLRRAQAQAGLADDAFVPALSASLGTSSSKQLDGDRISTRAS-----QSSVKL 130 DL +AA+ + +A+AQ + D P++SA+ GTSS+ + + +ST S Q SV + Sbjct: 95 DLRVAALNIEKARAQYRITDANRYPSISAT-GTSSASRTPAE-LSTTGSAAVSHQQSVTV 152 Query: 131 ---SYEVDLWGRLAAASDLSRWEALATEQDRQSTALTLIGTTANLYWTGAYLNQRVATAE 187 +YE+D +GRL SD + + R+ST ++L+ A + T A + A+ Sbjct: 153 GVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRDLLKLAQ 212 Query: 188 QSIAYAEKTLELVRTQYQAGAVSSVEVLSAQQNLYSQQSSYTELVQQRVENDNALAILFD 247 + A +T L + + G VS V++ S + + S ++ Q ++ NAL +L Sbjct: 213 DTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNALRLLVG 272 Query: 248 QPPGKAIALPARLPEQA--LPVLAAGVPAELLGRRPDLRALELRIRSALAGVDQARLNYY 305 A+ LPA LP+ A LP AG+P+++L RRPD+ A E ++SA A + AR ++ Sbjct: 273 ADIDAAL-LPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFF 331 Query: 306 PQLSLTGTLGTSSDALLRLVQNPVA--TLGGALTLPFLQHSEMKFKVGISQADFDTAVLN 363 P +SLT GT+S L L + T ++ LP ++ + ++ D V N Sbjct: 332 PSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRDIDVAN 391 Query: 364 FRKGLYTAFSEVENALSARQQYARQQALLVRNLDAAQAAERLLEVRYRAGSATLQLWLDQ 423 + K + TAF EV +AL+ R ++ + A++ A RL + RYR G + L Sbjct: 392 YEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYLTTLVA 451 Query: 424 QDKRRTAEISLAQVRLNQLKNQMTLYQALGG 454 Q +A+ +L +RL + N++TLY+ LGG Sbjct: 452 QRTLYSAQQTLISLRLTEQSNRITLYKVLGG 482 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,260,347 Number of extensions: 49647 Number of successful extensions: 283 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 17 Length of query: 465 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 376 Effective length of database: 1,198,455 Effective search space: 450619080 Effective search space used: 450619080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)