BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0050.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protei... 521 e-149 gi|300311353|ref|YP_003775445.1| flagellar basal body rod protei... 91 1e-19 gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protei... 83 2e-17 gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbasp... 75 4e-15 >gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protein FlgG [Herbaspirillum seropedicae SmR1] Length = 260 Score = 521 bits (1342), Expect = e-149, Method: Compositional matrix adjust. Identities = 260/260 (100%), Positives = 260/260 (100%) Query: 1 MIRSLWISKTGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQT 60 MIRSLWISKTGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQT Sbjct: 1 MIRSLWISKTGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQT 60 Query: 61 QLPSGLQIGTGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQILLPDGTTAYTRDGAF 120 QLPSGLQIGTGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQILLPDGTTAYTRDGAF Sbjct: 61 QLPSGLQIGTGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQILLPDGTTAYTRDGAF 120 Query: 121 QTDSQGQLVTSSGYTLQPPITVPLNTTKLTVGRDGVVSIMQAGSTATTQIGTIQVATFIN 180 QTDSQGQLVTSSGYTLQPPITVPLNTTKLTVGRDGVVSIMQAGSTATTQIGTIQVATFIN Sbjct: 121 QTDSQGQLVTSSGYTLQPPITVPLNTTKLTVGRDGVVSIMQAGSTATTQIGTIQVATFIN 180 Query: 181 PAGLESRGENLYMETAASGAPNPNNPGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAY 240 PAGLESRGENLYMETAASGAPNPNNPGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAY Sbjct: 181 PAGLESRGENLYMETAASGAPNPNNPGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAY 240 Query: 241 EINSKAITTSDQMLAKLSQL 260 EINSKAITTSDQMLAKLSQL Sbjct: 241 EINSKAITTSDQMLAKLSQL 260 >gi|300311353|ref|YP_003775445.1| flagellar basal body rod protein FlgF [Herbaspirillum seropedicae SmR1] Length = 245 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 80/267 (29%), Positives = 119/267 (44%), Gaps = 33/267 (12%) Query: 1 MIRSLWISKTGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQT 60 M R ++ + +G Q SNNLAN +T GF+ F Sbjct: 1 MDRLVYTAMSGAKHTLEQQATASNNLANATTTGFRAQLNTFR---------------AAP 45 Query: 61 QLPSGLQIGTGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQILLPDGTTAYTRDGAF 120 + GL T V F+QG + T D+AIQG G+ + DG AYTR+G+F Sbjct: 46 VVGDGLPTRTFVVDSTAGNDFSQGPIQDTGRALDVAIQGRGWIAVQTADGGEAYTRNGSF 105 Query: 121 QTDSQGQLVTSSGYTL---QPPITVPLNTTKLTVGRDGVVSIMQAGS--TATTQIGTIQV 175 + + G L T SG T+ PIT+P + T + + DG VS + A +G I+ Sbjct: 106 KINENGILQTQSGQTVLGDGGPITIPPDAT-VAIAADGTVSAIPTNGIPNAVNVLGRIK- 163 Query: 176 ATFINP--AGLESRGENLYMETAASGAPNPNNPGTNGAGALWQGYVETSNVNVVEELVNM 233 +NP ++ + L+ + P+ N L G +E SNVNVVE +VNM Sbjct: 164 --LVNPDEKNMKRGDDGLFRTITGAAQPDANV-------RLASGALEGSNVNVVEAMVNM 214 Query: 234 IQTQRAYEINSKAITTSDQMLAKLSQL 260 I R +E+N K I T++ K + + Sbjct: 215 INLSRQFEMNMKMIQTAESDATKATSI 241 >gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protein FlgE [Herbaspirillum seropedicae SmR1] Length = 432 Score = 83.2 bits (204), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 49/125 (39%), Positives = 70/125 (56%), Gaps = 13/125 (10%) Query: 10 TGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQTQLPSGLQIG 69 +GL+ LDVI NN+AN ST GFK+S+A F D+ ++ + G +G Sbjct: 8 SGLNGAAKSLDVIGNNVANASTVGFKQSQAQFADMYANSMNRSGNSP-----------VG 56 Query: 70 TGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQI--LLPDGTTAYTRDGAFQTDSQGQ 127 GV A + FTQGN++ +NN D+AI G+GFFQ+ L + + Y R+G FQ D G Sbjct: 57 IGVSVPAVAQQFTQGNISSSNNPLDIAINGDGFFQLSASLTNKSPMYGRNGQFQLDKSGY 116 Query: 128 LVTSS 132 +V S Sbjct: 117 IVNPS 121 Score = 72.0 bits (175), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 44/118 (37%), Positives = 63/118 (53%), Gaps = 1/118 (0%) Query: 143 PLNTTKLTVGRDGVVSIMQAGSTATTQIGTIQVATFINPAGLESRGENLYMETAASGAPN 202 P + + +V +DG + I + T +GT+ + F NP GL+ G NLY ++A+G+P Sbjct: 315 PGSLSSFSVDKDGSI-IGSYSNQQTKNLGTVVLVNFANPNGLQPLGNNLYQASSAAGSPL 373 Query: 203 PNNPGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAYEINSKAITTSDQMLAKLSQL 260 P T G L E SNV++ ELVNMI QR Y+ NS+ I D +L L L Sbjct: 374 VGKPTTGTFGTLQARAKEDSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQTLVSL 431 >gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbaspirillum seropedicae SmR1] Length = 420 Score = 75.5 bits (184), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 46/125 (36%), Positives = 64/125 (51%), Gaps = 13/125 (10%) Query: 10 TGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQTQLPSGLQIG 69 +GL LDVI NN+AN ST GFK+ + F D ++ + G +G Sbjct: 8 SGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSP-----------VG 56 Query: 70 TGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQIL--LPDGTTAYTRDGAFQTDSQGQ 127 GV + FTQGN+ TNN D+AI G+GFF++ L D + Y R+G FQ + G Sbjct: 57 IGVTVANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGY 116 Query: 128 LVTSS 132 L+ S Sbjct: 117 LINPS 121 Score = 73.6 bits (179), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 50/124 (40%), Positives = 68/124 (54%), Gaps = 7/124 (5%) Query: 138 PPITVPLNTTKLTVGRDGVVSIMQAGSTATTQI-GTIQVATFINPAGLESRGENLYMETA 196 PP T+ + ++G+DG SI+ A S T++ G I + F NP GL+ G NLY + Sbjct: 302 PPGTL----SSFSIGKDG--SIIGAYSNQQTRVMGQIVLVNFANPHGLQPIGNNLYQASE 355 Query: 197 ASGAPNPNNPGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAYEINSKAITTSDQMLAK 256 A+G+ P T GAL VE SNV++ ELVNMI QR Y+ NS+ I D +L Sbjct: 356 AAGSALVGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQT 415 Query: 257 LSQL 260 L L Sbjct: 416 LVSL 419 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.127 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 667,785 Number of extensions: 25228 Number of successful extensions: 67 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 63 Number of HSP's successfully gapped: 6 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 129 (54.3 bits)