BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0034.p (360 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 726 0.0 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 263 1e-71 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 236 3e-63 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 158 6e-40 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 93 3e-20 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 726 bits (1874), Expect = 0.0, Method: Compositional matrix adjust. Identities = 357/359 (99%), Positives = 357/359 (99%) Query: 2 MKIKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPR 61 MKIKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPR Sbjct: 1 MKIKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPR 60 Query: 62 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYAD 121 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYAD Sbjct: 61 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYAD 120 Query: 122 MIADKIRAAAKARVRARPARSAEQAAGGSGEALPQIRNPLTSSEKLIIIGASTGGTEAIK 181 MIADKIRAAAKARVRARPARSAEQAAGG EALPQIRNPLTSSEKLIIIGASTGGTEAIK Sbjct: 121 MIADKIRAAAKARVRARPARSAEQAAGGGSEALPQIRNPLTSSEKLIIIGASTGGTEAIK 180 Query: 182 EFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHS 241 EFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHS Sbjct: 181 EFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHS 240 Query: 242 HLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQGML 301 HLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQGML Sbjct: 241 HLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQGML 300 Query: 302 EMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQAMPGRVLAWLAEHGSRALRV 360 EMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQAMPGRVLAWLAEHGSRALRV Sbjct: 301 EMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQAMPGRVLAWLAEHGSRALRV 359 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 263 bits (673), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 145/351 (41%), Positives = 214/351 (60%), Gaps = 26/351 (7%) Query: 16 RSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLDFLEKLMRLR 75 R +M++++ PD+ V+G APDP+ A ++Q PDV+ LD++MPRMDG+ FL ++M R Sbjct: 19 RQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRMDGVSFLRQIMATR 78 Query: 76 PMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMIADKIRAAAKARV 135 P PV+M S+ + + AL GA+ + +P++ ++ +Q+ + +A IRAA +AR Sbjct: 79 PTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVEELASAIRAAMQARE 138 Query: 136 RAR----PARSA------------EQAAGG------SGEALPQIRNP----LTSSEKLII 169 R PAR AA G + +A+ Q P ++ +++ Sbjct: 139 GQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPPGPEDGLRPATARIVA 198 Query: 170 IGASTGGTEAIKEFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERV 229 IGASTGG +A++ L ++ CPGI++ QHMP+ FTRSFA+RL+ + V E+ +R+ Sbjct: 199 IGASTGGPQALERVLGKLSQRCPGIVVVQHMPQRFTRSFAERLHRISGAEVKEAEHLDRI 258 Query: 230 LPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVIL 289 LPG IAPG HL + R G Y + GP V+RH+PSVDVLF+S A AG NAVG+I+ Sbjct: 259 LPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGSNAVGIIM 318 Query: 290 TGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQ 340 TGMG DGA+GM E+ + G +AQDEAS VVFGMP+EAI G +V+ L+ Sbjct: 319 TGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDVITLE 369 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 236 bits (601), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 142/356 (39%), Positives = 207/356 (58%), Gaps = 11/356 (3%) Query: 4 IKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMD 63 IK+LI+DDSAL+R + ++ ++ + + A EL + PDV+TLD+ MP MD Sbjct: 2 IKLLIIDDSALMRRQLMQVFEQAGGFDIC-LGRNGREAVELNRSFAPDVVTLDINMPEMD 60 Query: 64 GLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMI 123 GL L +M RP+PVVMVSSLTE+G+ T A+ LGA+D+V KP +I + E + Sbjct: 61 GLSALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHEIEQDL 120 Query: 124 ADKIRAAAKARVRARP-----ARSAEQAAGGSGEALPQIRNPLTSSEKLIIIGASTGGTE 178 K+R AAKAR+R+R AR+ E A EA R +S+ L++IG STGG Sbjct: 121 LAKVRVAAKARIRSRAPAPELARAREPLAAPVAEA---ARRGRPASDGLVLIGVSTGGPS 177 Query: 179 AIKEFLIRMPSDCPG-ILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIA 237 ++E L +P+ P IL+ QHMP FTRSFA+R++ LC ++V+E A V GH YIA Sbjct: 178 TLEEILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIA 237 Query: 238 PGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSA-ATCAGKNAVGVILTGMGKDG 296 G + + + + G++ P + PSV++L +SA + A + +GV+LTGMG DG Sbjct: 238 RGGADMLVAKRGSHLSVLPKPESPTHLWHPSVELLARSALQSMAARQLIGVMLTGMGNDG 297 Query: 297 AQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQAMPGRVLAWLAE 352 A E+ G A+ E+SCVVFGMP++ I G A VL + ++ WL + Sbjct: 298 AAAFAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQLRVWLEQ 353 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 158 bits (400), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 110/349 (31%), Positives = 180/349 (51%), Gaps = 14/349 (4%) Query: 4 IKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMD 63 +K+ IV+D+ ++ V+R +I E++ +A D A ++ PDV+ +D+ MP++D Sbjct: 1 MKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD 60 Query: 64 GLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMI 123 G + ++M P P+++V+S + ++ A+ GA+D P + +G ++ A +I Sbjct: 61 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALI 120 Query: 124 ADKIRAAAKARVRARPARSAEQAAGGSGEAL-PQIRNPLTSSEKLIIIGASTGGTEAIKE 182 +KI R P + GG E L P S L+ +GAS GG A+ Sbjct: 121 -EKIDNLG----RLSPEMVSPPVRGGRAETLAPAQPARADSLPPLVAVGASAGGPAALAT 175 Query: 183 FLIRMPSDCPG-ILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHS 241 L +P G ++I QH+ F AQ L + V + + G +A + Sbjct: 176 LLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNR 235 Query: 242 HLKLVRSGA--NYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQG 299 HL +V+ G Y+ +G P + +RPSVDV FQS G++LTGMG+DGA G Sbjct: 236 HL-VVKEGGRLGYV----EGAPNDVYRPSVDVFFQSIVRHWPGKVAGLLLTGMGRDGAAG 290 Query: 300 MLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQAMPGRVLA 348 + +R++GA+ AQD+ SC V+GMP+ A+A+ AA EVL + M R+ A Sbjct: 291 LKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARLQA 339 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 61/174 (35%), Positives = 84/174 (48%), Gaps = 1/174 (0%) Query: 168 IIIGASTGGTEAIKEFLIRMPSDCP-GILITQHMPEGFTRSFAQRLNNLCKISVVESAGN 226 I+IGAS GG EA+ E L + ++ H+P AQ + C+ VVE+ Sbjct: 17 IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQPVVEAQDK 76 Query: 227 ERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVG 286 E G + AP HL + RS + + VN RP++DVLF+SAA G +G Sbjct: 77 EPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLFESAADVYGPRLLG 136 Query: 287 VILTGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQ 340 ++LTG DGA GM + AG Q V MP +A+A+G VL LQ Sbjct: 137 ILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQ 190 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,039,005 Number of extensions: 41262 Number of successful extensions: 236 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 238 Number of HSP's successfully gapped: 14 Length of query: 360 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 274 Effective length of database: 1,212,660 Effective search space: 332268840 Effective search space used: 332268840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)