BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0032.p (290 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransf... 604 e-174 gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransf... 138 7e-34 gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransf... 136 2e-33 gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis... 78 7e-16 gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransfe... 71 1e-13 >gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 290 Score = 604 bits (1558), Expect = e-174, Method: Compositional matrix adjust. Identities = 289/290 (99%), Positives = 290/290 (100%) Query: 1 VKPTVPSKGESSGKEFDFNSRDFERVRGLIYQRAGIALADSKQEMVYSRLARRLRATGIT 60 +KPTVPSKGESSGKEFDFNSRDFERVRGLIYQRAGIALADSKQEMVYSRLARRLRATGIT Sbjct: 1 MKPTVPSKGESSGKEFDFNSRDFERVRGLIYQRAGIALADSKQEMVYSRLARRLRATGIT 60 Query: 61 SFQQYLDMLERTHDSEEWEAFTNALTTNLTSFFREAHHFPILADFLKTLKEPATIWCSAA 120 SFQQYLDMLERTHDSEEWEAFTNALTTNLTSFFREAHHFPILADFLKTLKEPATIWCSAA Sbjct: 61 SFQQYLDMLERTHDSEEWEAFTNALTTNLTSFFREAHHFPILADFLKTLKEPATIWCSAA 120 Query: 121 STGEEPYTIAMTACEAYGSLTPPVQIVATDIDTNVLATGSNGVYPMERIEKMSPERVKKF 180 STGEEPYTIAMTACEAYGSLTPPVQIVATDIDTNVLATGSNGVYPMERIEKMSPERVKKF Sbjct: 121 STGEEPYTIAMTACEAYGSLTPPVQIVATDIDTNVLATGSNGVYPMERIEKMSPERVKKF 180 Query: 181 FLRGKGSHEGYVRVRPELRKLITFKQLNLLADGWPLSGQFDVIFCRNVMIYFDKPTQSKI 240 FLRGKGSHEGYVRVRPELRKLITFKQLNLLADGWPLSGQFDVIFCRNVMIYFDKPTQSKI Sbjct: 181 FLRGKGSHEGYVRVRPELRKLITFKQLNLLADGWPLSGQFDVIFCRNVMIYFDKPTQSKI 240 Query: 241 LSRFVPLMKPHALLFAGHSENFLYVSDALKLKGKTVYELNATQRGARTKP 290 LSRFVPLMKPHALLFAGHSENFLYVSDALKLKGKTVYELNATQRGARTKP Sbjct: 241 LSRFVPLMKPHALLFAGHSENFLYVSDALKLKGKTVYELNATQRGARTKP 290 >gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 138 bits (347), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 85/252 (33%), Positives = 136/252 (53%), Gaps = 5/252 (1%) Query: 29 LIYQRAGIALADSKQEMVYSRLARRLRATGITSFQQYLDMLERTHDSEEWEAFTNALTTN 88 L Q+ G+ L SK+ ++ SRLA RL G+ QY ++ ++E + + +TTN Sbjct: 24 LFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYHALISSEAGAQERQRAIDLITTN 83 Query: 89 LTSFFREAHHFP-ILADFLKTL-KEPAT-IWCSAASTGEEPYTIAMTACEAYGSLTPPVQ 145 T FFRE HF ++ +L L ++P IW +A++TGEE YT+AM + V Sbjct: 84 ETYFFREPGHFDFVVGQWLPQLAQQPQLRIWSAASATGEEAYTLAMLLDHHRPGASWSV- 142 Query: 146 IVATDIDTNVLATGSNGVYPMERIEKMSPERVKKFFLRGKGSHEGYVRVRPELRKLITFK 205 A+DI T VL +YPM R + + ++++ L G G +EG+ V+ +LR + F Sbjct: 143 -FASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMGVGEYEGHFLVQADLRAKVQFA 201 Query: 206 QLNLLADGWPLSGQFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHALLFAGHSENFLYV 265 Q NL GQ+D+IF RNV+IYFD T+ ++L + ++P L GHSE+ + Sbjct: 202 QRNLTRLDEHDDGQYDLIFLRNVIIYFDCETKLQVLRDVIARLRPGGYLVVGHSESLHGM 261 Query: 266 SDALKLKGKTVY 277 L ++ ++Y Sbjct: 262 DLGLDMQSPSIY 273 >gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 136 bits (342), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 81/260 (31%), Positives = 130/260 (50%), Gaps = 13/260 (5%) Query: 22 DFERVRGLIYQRAGIALADSKQEMVYSRLARRLRATGITSFQQYLDMLERTHDSEEWEAF 81 DF + R Y++ GI+ +K+ V RL R+ ATG SF+ Y +L D +E + Sbjct: 10 DFLKFREYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFEADGKELQLL 69 Query: 82 TNALTTNLTSFFREAHHFPILADFL-------KTLKEPATIWCSAASTGEEPYTIAMTAC 134 TN +T N T F RE + F L + + + P IWC S+GEE YT+AM Sbjct: 70 TNLMTVNETYFLREEYQFQCLVNSMLPEITRNRRSTAPIRIWCIPCSSGEEAYTLAMYLM 129 Query: 135 EAY-GSLTPPVQIVATDIDTNVLATGSNGVYPMERIEKMSPERVKKFFLRGKGSHEGYVR 193 E + G V+I+++DIDT VL G Y ++ + + + K+FLR ++ Sbjct: 130 ERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQGDEYQ---- 185 Query: 194 VRPELRKLITFKQLNLL-ADGWPLSGQFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHA 252 + LR + F ++NL + FDVIFCRN++IYFD ++ + F + + Sbjct: 186 ICDALRGAVEFTRVNLAKSQDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEALNLNG 245 Query: 253 LLFAGHSENFLYVSDALKLK 272 + GHSE+ +S +++ Sbjct: 246 FICLGHSESMSRISSLYRVR 265 >gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis proteins protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 78.2 bits (191), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 61/192 (31%), Positives = 91/192 (47%), Gaps = 15/192 (7%) Query: 90 TSFFREAHHFPILADFL--KTLKEPAT---IWCSAASTGEEPYTIAMTACEAYGSLTPPV 144 T FFR+ +A K +PA I STGEEPY++AM +A LTP Sbjct: 69 TWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMALLDA--GLTPGQ 126 Query: 145 QIV-ATDIDTNVLATGSNGVYPMERIEKMSPE-RVKKFFLRGKGSHEGYVRVRPELRKLI 202 ++ A DI T L + G+Y R + F L +G R+ +R + Sbjct: 127 FVIDAYDIRTRSLEIAAAGLYGRNSFRGQELAFRDRHFDL-----EQGQWRLHERIRSQV 181 Query: 203 TFKQLNLLADGWPLSGQ-FDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHALLFAGHSEN 261 F NLLA + Q +D IFCRNV+IYF++ Q +++ +MKP AL+F G +E Sbjct: 182 RFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPDALIFVGPAEG 241 Query: 262 FLYVSDALKLKG 273 + + ++ G Sbjct: 242 GILLGPTMESAG 253 >gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransferase chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 70.9 bits (172), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 67/231 (29%), Positives = 102/231 (44%), Gaps = 17/231 (7%) Query: 50 LARRLRATGITSFQ-QYLDMLER--THDSEEWEAFTNALTTNLTSFFREAHHFPILAD-- 104 L RRL AT + F Q L L+ HD+ + LT ++ FR+ +F L + Sbjct: 41 LKRRL-ATAMIRFDCQTLSQLQDRVLHDAAVFPLLMEYLTVQVSEMFRDPSYFLALREKV 99 Query: 105 --FLKTLKEPATIWCSAASTGEEPYTIAMTACEAYGSLTPPVQIVATDIDTNVLATGSNG 162 L+T IW + S+GEE Y++A+ E G L + I ATDI+ + L G Sbjct: 100 VPLLRTYP-SLKIWVAGCSSGEEVYSLAILLREE-GLLERSI-IYATDINQSALKKAEAG 156 Query: 163 VYPMERIEKMSPERVKKFFLRGKGSHE----GYVRVRPELRKLITFKQLNLLADGWPLSG 218 VY +ERI + K + G V LRK + F +L D + Sbjct: 157 VYDLERIAGFTNNHQKSGARTSLSDYYTAAYGAVVFDKSLRKNVVFSDHSLATD--SVFA 214 Query: 219 QFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHALLFAGHSENFLYVSDAL 269 + ++ CRNV+IYF++ Q + L F + L G E+ + S A+ Sbjct: 215 EMHLVSCRNVLIYFNRQLQERALGLFRESLVRKGFLGLGSKESLRFSSHAV 265 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 860,321 Number of extensions: 33926 Number of successful extensions: 112 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 103 Number of HSP's successfully gapped: 5 Length of query: 290 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 206 Effective length of database: 1,222,130 Effective search space: 251758780 Effective search space used: 251758780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)