BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0026.p (292 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 560 e-161 gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 117 1e-27 gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 107 9e-25 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 68 7e-13 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 560 bits (1444), Expect = e-161, Method: Compositional matrix adjust. Identities = 288/292 (98%), Positives = 291/292 (99%) Query: 1 MKIAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADTDVL 60 MKIAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAAD DVL Sbjct: 1 MKIAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADADVL 60 Query: 61 ISMLADDDATETSLLDAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVL 120 ISMLADDDATETSLLDAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVL Sbjct: 61 ISMLADDDATETSLLDAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVL 120 Query: 121 GRVNVAEAGQLNILAAGEEQALAAVQPLLEVLGQKTWRLGRQPEQANAAKLAMNFMIASA 180 GRVNVAEAGQLNILAAGEEQALAAVQPLL+VLGQKTWRLGRQPEQANAAKLAMNFMIASA Sbjct: 121 GRVNVAEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIASA 180 Query: 181 IGAMSEAVALVQGYGLDKAGFIELATSTAFAAPVYKGYGQAIADERFEPAGFKLALGLKD 240 IGAMSEAVALVQGYGLDKAGFIELATSTAFAAPVYKGYGQAIAD+RFEPAGFKLALGLKD Sbjct: 181 IGAMSEAVALVQGYGLDKAGFIELATSTAFAAPVYKGYGQAIADDRFEPAGFKLALGLKD 240 Query: 241 VRLALEAGEQAHVPLALASALRDLHIDGLAHGEGHLDWAALSRASARRAGQA 292 VRLALEAGEQAHVPL+LASALRDLHIDGLAHGEGHLDWAALSRASARRAGQA Sbjct: 241 VRLALEAGEQAHVPLSLASALRDLHIDGLAHGEGHLDWAALSRASARRAGQA 292 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 117 bits (293), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 88/287 (30%), Positives = 146/287 (50%), Gaps = 8/287 (2%) Query: 2 KIAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADTDVLI 61 I F+GLG MG PMA NLV+AG++V + A V++L +G + A A A DV+I Sbjct: 4 NIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKADVVI 63 Query: 62 SMLADDDATETSLL-DAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVL 120 +ML E+ L +AG LA+ +PG + ++ +TI+ A ++A +ARG + APV Sbjct: 64 TMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVS 123 Query: 121 GRVNVAEAGQLNILAAGEEQALAAVQPLLEVLGQKTWRLGRQPEQANAAKLAMNFMIASA 180 G N A AG L + G ++A A QP L+ +G+ + G + K+ N ++ Sbjct: 124 GGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAG-ESGSGQTVKVCNNMLLGIL 182 Query: 181 IGAMSEAVALVQGYGLDKAGFIELATSTA---FAAPVYK---GYGQAIADERFEPAGFKL 234 + SEA+ L G+D E+ + ++ + VY G + + GF + Sbjct: 183 MIGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGGFGV 242 Query: 235 ALGLKDVRLALEAGEQAHVPLALASALRDLHIDGLAHGEGHLDWAAL 281 L LKD+ LA+E + + L + R+L+ +G G LD++++ Sbjct: 243 DLMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSI 289 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 107 bits (268), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 77/283 (27%), Positives = 148/283 (52%), Gaps = 2/283 (0%) Query: 2 KIAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADTDVLI 61 + +GLG MG+ +A++L+RAG+ V A + +++ AA G A A PAE A DV+I Sbjct: 4 NVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVI 63 Query: 62 SMLADDDATETSLL-DAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVL 120 +++ + TET L D GA++A++PG++ ++ AT++ A+ L +G+ + APV Sbjct: 64 TLVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDAPVS 123 Query: 121 GRVNVAEAGQLNILAAGEEQALAAVQPLLEVLGQKTWRLGRQPEQANAAKLAMNFMIASA 180 G A +G++ ++ +G + A ++ +L + K +RLG + K+ + Sbjct: 124 GGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVH 183 Query: 181 IGAMSEAVALVQGYGLDKAGFIELATSTAFAAPVYKGYGQAIADERFEPAGFKLALGLKD 240 I A +EA+AL G++ E+ T +A + +++ I + P + + +KD Sbjct: 184 IAASAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHILSGDYTPLS-AVDIFVKD 242 Query: 241 VRLALEAGEQAHVPLALASALRDLHIDGLAHGEGHLDWAALSR 283 + L L+ ++ PL L++A + + G G D +A+ + Sbjct: 243 LGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIK 285 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 68.2 bits (165), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 84/278 (30%), Positives = 127/278 (45%), Gaps = 9/278 (3%) Query: 2 KIAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADTDVLI 61 KI +GLG MG MA +L R GY V ++ + LA G + A A ++++ Sbjct: 3 KIGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHCVDSIAALAPAVEMVV 62 Query: 62 SMLADDDATETSLL-DAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVL 120 L D E + + G L PG + V+ T RLA QA G+ V APV Sbjct: 63 LSLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAPVS 122 Query: 121 GRVNVAEAGQLNILAAGEEQALAAVQPLLEVLGQKTWRLGRQPEQA-NAAKLAMNFMIAS 179 G A + ++ G + +A ++P+L V+ K +G P A + AKL N + + Sbjct: 123 GGPKGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVG--PVGAGHVAKLLNNLVTGT 180 Query: 180 AIGAMSEAVALVQGYGLD-KAGFIELATSTAFAAPVYKGYGQAIADERFEPAGFKLALGL 238 + EA + GLD + F LA +A + + Y I +E F+ +GF + L Sbjct: 181 HLLIAGEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTWIFNEAFD-SGFTMKLMR 239 Query: 239 KDVRLALEAGEQAH--VPLAL-ASALRDLHIDGLAHGE 273 KD+RLA+ E+ PLA A L ++GLA E Sbjct: 240 KDLRLAMNLIEETRSTTPLAREAGRLWARSVEGLADAE 277 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 871,362 Number of extensions: 36195 Number of successful extensions: 246 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 241 Number of HSP's successfully gapped: 4 Length of query: 292 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 208 Effective length of database: 1,222,130 Effective search space: 254203040 Effective search space used: 254203040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)