BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0019.p (343 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 708 0.0 gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 449 e-127 gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [H... 92 6e-20 gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herb... 89 4e-19 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 85 8e-18 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 708 bits (1827), Expect = 0.0, Method: Compositional matrix adjust. Identities = 343/343 (100%), Positives = 343/343 (100%) Query: 1 MTQATILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFVQG 60 MTQATILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFVQG Sbjct: 1 MTQATILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFVQG 60 Query: 61 DIRDRAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVK 120 DIRDRAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVK Sbjct: 61 DIRDRAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVK 120 Query: 121 NIVFSSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYF 180 NIVFSSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYF Sbjct: 121 NIVFSSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYF 180 Query: 181 NPVGAHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVD 240 NPVGAHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVD Sbjct: 181 NPVGAHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVD 240 Query: 241 LALGHLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRPGDIAC 300 LALGHLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRPGDIAC Sbjct: 241 LALGHLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRPGDIAC 300 Query: 301 CYAATERAERELGWRAQRGIEAMCADTWRWQALAKRLAQEEAA 343 CYAATERAERELGWRAQRGIEAMCADTWRWQALAKRLAQEEAA Sbjct: 301 CYAATERAERELGWRAQRGIEAMCADTWRWQALAKRLAQEEAA 343 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 449 bits (1154), Expect = e-127, Method: Compositional matrix adjust. Identities = 211/329 (64%), Positives = 256/329 (77%), Gaps = 1/329 (0%) Query: 5 TILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFVQGDIRD 64 ILVTGGAGYIGSHTCVEL+ AG+ V+V DN CNS + R+ QI+G+ V+GDIR+ Sbjct: 2 NILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIRN 61 Query: 65 RAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVKNIVF 124 R L+ + + + EAVIHFAGLKAVGESV QPL YYDNNV G+ L EAM A GVK IVF Sbjct: 62 REQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIVF 121 Query: 125 SSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYFNPVG 184 SSSATVYGDP +P+ E PLSA+NPYGRSK+++E +L D + A P W+IALLRYFNPVG Sbjct: 122 SSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPVG 181 Query: 185 AHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVDLALG 244 AHESGLIGEDP GIPNNL+PF+AQVA GRR L+++G DYPTPDGTGVRD+IHVVDLA G Sbjct: 182 AHESGLIGEDPGGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLARG 241 Query: 245 HLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRPGDIACCYAA 304 H+ L+ L+ P + NLGTG G SVL++L A+E+ACG LPY + +RRPGDIA CYA Sbjct: 242 HVAALQALD-APQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIASCYAN 300 Query: 305 TERAERELGWRAQRGIEAMCADTWRWQAL 333 A+R+LGW A G+EAMCAD+W+WQ + Sbjct: 301 PAEAKRKLGWEATLGLEAMCADSWKWQQM 329 >gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [Herbaspirillum seropedicae SmR1] Length = 338 Score = 92.0 bits (227), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 96/347 (27%), Positives = 142/347 (40%), Gaps = 44/347 (12%) Query: 6 ILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQ-RPHFVQGDIRD 64 + VTGGAG+IG++ + + A + VL+ + A D +A ++G R F Q DI D Sbjct: 2 LFVTGGAGFIGANYILHFLAAPHDEAVLNIDKLTYAGNPDNLASVAGDARYLFSQTDICD 61 Query: 65 RAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEA---------MA 115 A + +F HR AV+H A V S+A P + NV+G+ + A A Sbjct: 62 TAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSAAYDYWSTLPAA 121 Query: 116 AHGVKNIVFSSSATVYGDPA--SVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWR 173 + S+ V+G A + P TE P + +PY SK + ++ H Sbjct: 122 EQAAFRFLHISTDEVFGSLAADAPPFTETSPYAPNSPYSASKAASDHLVRAFHST----- 176 Query: 174 IALLRYFNPVG-AHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGV 232 Y PV H S G P P L+P + A + L +YG DG V Sbjct: 177 -----YGLPVLITHCSNNYG--PFHFPEKLIPLVITNALAGQ-PLPIYG------DGQQV 222 Query: 233 RDYIHVVDLALGHLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAF-----EQACGNKLP 287 RD++HV D L + G +YN+G + LE++ E A + Sbjct: 223 RDWLHVADHCRAIELVLARARPGE---SYNIGGNSEKTNLEVVLTLCDLLDELAPKAQGQ 279 Query: 288 YQ----LVDRRPGDIACCYAATERAERELGWRAQRGIEAMCADTWRW 330 Y+ V RPG T + +RELGW Q T +W Sbjct: 280 YRDQICFVQDRPGHDRRYAVDTSKIQRELGWSPQHAFRQGLRQTVQW 326 >gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 92/348 (26%), Positives = 144/348 (41%), Gaps = 35/348 (10%) Query: 1 MTQATILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRP----H 56 +T T LVTG AG+IGS+ L+ VV LDN +D + + Sbjct: 14 LTPRTWLVTGVAGFIGSNLLETLLKLNQRVVGLDNFSTGFQHNLDEVKALVTPEQWANFR 73 Query: 57 FVQGDIRD----RAVLD-GIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGS-NVL 110 FV+GDIR RA + S+ +E V+H A L +V S+ P+ NN+ G N+L Sbjct: 74 FVEGDIRKLDDCRAAMHHEALGSNIVEYVLHQAALGSVPRSIEDPITTNQNNIDGFLNML 133 Query: 111 FEAMAAHGVKNIVFSSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADP 170 A A VK V+++S++ YGD +P E+ +PY +K V ++ D+ Sbjct: 134 VAARDAR-VKRFVYAASSSTYGDHPGLPKVEDRIGKPLSPYAVTKF-VNELYADVFARTY 191 Query: 171 SWRIALLRYFNPVGAHESGLIGEDPSGIPNNLLP-FIAQVADGRRAALSVYGSDYPTPDG 229 + LRYFN G ++P G ++P +IA G + Y DG Sbjct: 192 GLQTIGLRYFNIFGRR------QNPDGAYAAVIPKWIAAAIKGEQI--------YINGDG 237 Query: 230 TGVRDYIHVVDLALGHLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQ 289 RD+ +V + +L L+ YN+ G ++ E+ P+ Sbjct: 238 ETSRDFCYVDNAVQANLLAACTLQDEAANQVYNVAVGDRTTLKELYQFLRDNLSATFPH- 296 Query: 290 LVDRRP-------GDIACCYAATERAERELGWRAQRGIEAMCADTWRW 330 L D +P GD+ A + R LG+ I ++ W Sbjct: 297 LRDAQPIFREFRAGDVRHSLADIGKGRRLLGYEPSHRIGRGLKESMNW 344 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 85.1 bits (209), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 86/337 (25%), Positives = 141/337 (41%), Gaps = 33/337 (9%) Query: 7 LVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRP----HFVQGDI 62 L+TG AG+IGS+ L+ G V LDN R + ++ + G F++GDI Sbjct: 19 LITGVAGFIGSNLLARLLTLGQQVTGLDNFSTGRQANLEEVRAEVGAAAWANFRFIEGDI 78 Query: 63 RDRAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVKNI 122 D A + + V+H A L +V S+ P+R NNV+G + A GV Sbjct: 79 CDAATC--LRACDGADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAGVARF 136 Query: 123 VFSSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYFNP 182 V+++S++ YGD +P EE +PY +K V ++ ++ LRYFN Sbjct: 137 VYAASSSTYGDHPGLPKVEEVIGKPLSPYAVTK-YVNELYAEVFARCYGMESIGLRYFNV 195 Query: 183 VGAHESGLIGEDPSGIPNNLLP--FIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVD 240 G +DP G ++P A + +G L + G DG RD+ ++ + Sbjct: 196 FGRR------QDPHGAYAAVIPQWVTAMIRNGE---LRING------DGQTSRDFCYIDN 240 Query: 241 LALGHLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRP----- 295 + +L E+ YN+ S+ ++ A + + P+ L + RP Sbjct: 241 VVQANLLAATAGEEAVN-QVYNVALNDRTSLNDLYAMMRELLVQRFPH-LQEHRPSYVGF 298 Query: 296 --GDIACCYAATERAERELGWRAQRGIEAMCADTWRW 330 GDI A +A + LG+ + A W Sbjct: 299 RAGDIRDSQADIGKARQRLGYAPTHRLRAGLEQAMSW 335 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,174,671 Number of extensions: 52502 Number of successful extensions: 208 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 6 Length of query: 343 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 257 Effective length of database: 1,212,660 Effective search space: 311653620 Effective search space used: 311653620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)