BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0004.p (325 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312976|ref|YP_003777068.1| GGDEF family protein [Herbaspir... 151 6e-38 gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709... 56 4e-09 >gi|300312976|ref|YP_003777068.1| GGDEF family protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 151 bits (382), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 73/74 (98%), Positives = 73/74 (98%) Query: 1 MRIAELPANEAARIDALHSLRILDTPREERFDRLTRLARRLFDVPMAVVTMIDVNRQWFK 60 MRIAELPANEAARIDALHSLRILDTPREERFDRLTRLARRLFDVPMAVVTMIDVNRQWFK Sbjct: 1 MRIAELPANEAARIDALHSLRILDTPREERFDRLTRLARRLFDVPMAVVTMIDVNRQWFK 60 Query: 61 SCVGLGDATETSRR 74 SCVGLGDATETSR Sbjct: 61 SCVGLGDATETSRE 74 >gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709 [Herbaspirillum seropedicae SmR1] Length = 598 Score = 56.2 bits (134), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 30/65 (46%), Positives = 45/65 (69%), Gaps = 1/65 (1%) Query: 9 NEAARIDALHSLRILDTPREERFDRLTRLARRLFDVPMAVVTMIDVNRQWFKSCVGLGDA 68 E+ R+ LH LDTP ++ D++T+LA +FDV +A+V ++D +RQWFKS VGL + Sbjct: 17 QESQRLAQLHGYCALDTPPDKLCDQVTQLACSMFDVEIALVALVDEHRQWFKSRVGL-EV 75 Query: 69 TETSR 73 +ET R Sbjct: 76 SETPR 80 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.336 0.147 0.503 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 903,379 Number of extensions: 30917 Number of successful extensions: 98 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 2 Length of query: 325 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 240 Effective length of database: 1,217,395 Effective search space: 292174800 Effective search space used: 292174800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.7 bits) S2: 131 (55.1 bits)