BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0025.p (511 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 1043 0.0 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 163 4e-41 gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 162 9e-41 gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 101 1e-22 gi|300310315|ref|YP_003774407.1| cysteine synthase B protein [He... 58 2e-09 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 1043 bits (2696), Expect = 0.0, Method: Compositional matrix adjust. Identities = 508/508 (100%), Positives = 508/508 (100%) Query: 4 MSNDYLKKILTARVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMA 63 MSNDYLKKILTARVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMA Sbjct: 1 MSNDYLKKILTARVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMA 60 Query: 64 HLTPAQLKRGVICASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLF 123 HLTPAQLKRGVICASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLF Sbjct: 61 HLTPAQLKRGVICASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLF 120 Query: 124 GDSFTDAYEHALTLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGG 183 GDSFTDAYEHALTLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGG Sbjct: 121 GDSFTDAYEHALTLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGG 180 Query: 184 GLIAGVASYVKAVRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEE 243 GLIAGVASYVKAVRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEE Sbjct: 181 GLIAGVASYVKAVRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEE 240 Query: 244 TFRIAKELVDEVIIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPI 303 TFRIAKELVDEVIIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPI Sbjct: 241 TFRIAKELVDEVIIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPI 300 Query: 304 KGESLITIACGANMNFDRLRFVAEMADAGEAREAVFAVTIPEERGSFRRFCETVGPRNVT 363 KGESLITIACGANMNFDRLRFVAEMADAGEAREAVFAVTIPEERGSFRRFCETVGPRNVT Sbjct: 301 KGESLITIACGANMNFDRLRFVAEMADAGEAREAVFAVTIPEERGSFRRFCETVGPRNVT 360 Query: 364 EFNYRISDAKAAHVFVGIQVSAADEAGKIARNFEKAGFGVLDLTHDELAKVHIRHLVGGK 423 EFNYRISDAKAAHVFVGIQVSAADEAGKIARNFEKAGFGVLDLTHDELAKVHIRHLVGGK Sbjct: 361 EFNYRISDAKAAHVFVGIQVSAADEAGKIARNFEKAGFGVLDLTHDELAKVHIRHLVGGK 420 Query: 424 SDLTGDELLYRFEFPERPGALMKFLSSMNPGWNISLFHYRNQGGDVGRILIGLQVPKKEM 483 SDLTGDELLYRFEFPERPGALMKFLSSMNPGWNISLFHYRNQGGDVGRILIGLQVPKKEM Sbjct: 421 SDLTGDELLYRFEFPERPGALMKFLSSMNPGWNISLFHYRNQGGDVGRILIGLQVPKKEM 480 Query: 484 KEFRAFLAQLGYRHWDESKNPLYKLFLG 511 KEFRAFLAQLGYRHWDESKNPLYKLFLG Sbjct: 481 KEFRAFLAQLGYRHWDESKNPLYKLFLG 508 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 163 bits (412), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 107/258 (41%), Positives = 154/258 (59%), Gaps = 4/258 (1%) Query: 23 ETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQL-KRGVICASAGN 81 ETP + TLSQ + +++ K E++Q SFK RGA NK+ + + RGV+ SAGN Sbjct: 20 ETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLVDVAASGGGARGVVAMSAGN 79 Query: 82 HAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQ 141 HAQGVA A RLG A IVMP TP VKI+ + G VVL GD+ A HA L +K+ Sbjct: 80 HAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVLHGDTLEAARTHAYELAEKE 139 Query: 142 KLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVKAVRPDIK 201 LTFVHP+DD +IAGQGTV +E+++ PD + + V+IGGGGL+AG+A +A++P+I+ Sbjct: 140 GLTFVHPYDDEAIIAGQGTVALEMMKAVPD-LDCLVVSIGGGGLLAGMAVAARAIKPEIE 198 Query: 202 IIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTD 261 I+GVQT M ++K ++G AV G T I DE+++VD Sbjct: 199 IVGVQTRRFPTMVNAVKGTEHPV--GASTIAEGIAVGTPGTLTRAIIAREADELLLVDEG 256 Query: 262 AVCTAIKDIFQDTRSIVE 279 + A+ + + +++VE Sbjct: 257 DIEQAVLMLLEIEKTLVE 274 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 162 bits (409), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 108/308 (35%), Positives = 171/308 (55%), Gaps = 10/308 (3%) Query: 16 RVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQLKRGVI 75 R+ A TP+ + T + +++FK E+ Q + +FK RGA N + Q K GVI Sbjct: 22 RIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNALTQFDATQRKHGVI 81 Query: 76 CASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEH-A 134 S+GNHAQG AL+A LG AVIVMP P K+ A + G EVV + D +T+ E Sbjct: 82 TFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVTY-DRYTEDREAIG 140 Query: 135 LTLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVK 194 L ++K+T + P+D P+V+AGQGT +E+L Q P+ A+FV +GGGGL++G A + Sbjct: 141 ARLAAERKMTLIPPYDHPHVMAGQGTATLELL-QETGPLDALFVCLGGGGLLSGAALAAR 199 Query: 195 AVRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDE 254 A+ P KI GV+ + +SL+AG+ V + +DG + +G+ TF + +ELVD+ Sbjct: 200 ALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKYTFGVIRELVDD 259 Query: 255 VIIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESLITIACG 314 + V + + ++ + + +VEP G L A A+ K ++G+ + I G Sbjct: 260 IHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKL-------KDQLQGKRVGVIISG 312 Query: 315 ANMNFDRL 322 N++ +L Sbjct: 313 GNVDLGKL 320 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 101 bits (252), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 75/247 (30%), Positives = 125/247 (50%), Gaps = 3/247 (1%) Query: 32 LSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQLK-RGVICASAGNHAQGVALSA 90 L+Q + ++ K E+ +FK+RG + HL Q + GV+ A+ GNH Q V L+A Sbjct: 37 LNQALGAEVWVKHENHAPTGAFKVRGGMVYLHHLARQQPQLSGVVSATRGNHGQSVGLAA 96 Query: 91 ARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQKLTFVHPFD 150 RLG A IV+P + K A++A G E++ G F ++ E+A L +Q+L + P Sbjct: 97 RRLGISATIVVPQGNSREKNAAMRALGVELIEHGSEFQESREYAQQLAAQQQLHMI-PSL 155 Query: 151 DPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVKAVRPDIKIIGVQTTDS 210 ++AG + ME+ P + + V IG G + G + A+ +IIGV + + Sbjct: 156 HRDLVAGVASYWMELFAAQPG-LDVVLVPIGQGSGMCGAVAARNALGLSTRIIGVVSAHA 214 Query: 211 DAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTDAVCTAIKDI 270 A S +AGR++ P +DG A ++ + + + VDEV+ V D V A+K Sbjct: 215 LAYKLSFEAGRKIESPVSTCIADGVACRVPDQASLEVLFSEVDEVVAVTDDEVMDAMKLA 274 Query: 271 FQDTRSI 277 + T ++ Sbjct: 275 YIATHNV 281 >gi|300310315|ref|YP_003774407.1| cysteine synthase B protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 58.2 bits (139), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 55/191 (28%), Positives = 89/191 (46%), Gaps = 14/191 (7%) Query: 33 SQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTP-AQLKRG--VICASAGNHAQGVALS 89 ++R +N I K E S K R A++ + Q+K G +I A++GN +A+ Sbjct: 26 AKRRNNLILGKLEGNNPAGSVKDRAAFSMITRAEARGQIKPGDTLIEATSGNTGIALAMV 85 Query: 90 AARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQKL------ 143 AA G + +++MP + + ++ A G ++VL S Y L E+ QK Sbjct: 86 AAMRGYKMILLMPENLSEERRQSMAAYGAKIVLTPKSGGMEYARDLA-EQMQKNGEGLIL 144 Query: 144 -TFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVKAVRPDIKI 202 F +P D+P +A T G EI R I A+G G I GVA Y+K ++I Sbjct: 145 DQFANP-DNP--LAHYETTGPEIWRDTKGSITHFVSAMGTTGTIMGVAQYLKEQNAQVQI 201 Query: 203 IGVQTTDSDAM 213 +G Q + ++ Sbjct: 202 VGAQPEEGSSI 212 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,447,286 Number of extensions: 57728 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 5 Length of query: 511 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 422 Effective length of database: 1,198,455 Effective search space: 505748010 Effective search space used: 505748010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)