BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0022.p (1333 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313195|ref|YP_003777287.1| oxidoreductase [Herbaspirillum ... 2762 0.0 gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked s... 92 3e-19 gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbas... 84 9e-17 gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenas... 75 4e-14 gi|300313119|ref|YP_003777211.1| glycolate oxidase iron-sulfur s... 65 3e-11 >gi|300313195|ref|YP_003777287.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 1333 Score = 2762 bits (7160), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1330/1333 (99%), Positives = 1332/1333 (99%) Query: 1 MNAPAQIQALLTDAPHGATPPRLREIPYNYTSFSDREIVIRLLGEESWSLLDELRGKRQT 60 MNAPAQIQALLTDAPHGATPPRLREIPYNYTSFSDREIVIRLLGEESWSLLDELRGKRQT Sbjct: 1 MNAPAQIQALLTDAPHGATPPRLREIPYNYTSFSDREIVIRLLGEESWSLLDELRGKRQT 60 Query: 61 GRSARMLYEVLGDIWVVRRNPYLQDDLLDNPKRRAALIEALNHRLGEVDKRRLATDQAEA 120 GRSARMLYEVLGDIWVVRRNPYLQDDLLDNPKRRAALIEALNHRLGEVDKRRLATDQAEA Sbjct: 61 GRSARMLYEVLGDIWVVRRNPYLQDDLLDNPKRRAALIEALNHRLGEVDKRRLATDQAEA 120 Query: 121 GDAEAQRRSASVEALLKAAKKAIADFAEEFRQTYDLRKRATKVLGRFTAKDNIKFDGLSR 180 GDAEAQRRSASVEALLKAAKKAIADFAEEFRQTYDLRKRATKVLGRFTAKDNIKFDGLSR Sbjct: 121 GDAEAQRRSASVEALLKAAKKAIADFAEEFRQTYDLRKRATKVLGRFTAKDNIKFDGLSR 180 Query: 181 VSHVTDATDWRVEYPFVVLTPDTEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPLTPL 240 VSHVTDATDWRVEYPFVVLTPDTEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPLTP+ Sbjct: 181 VSHVTDATDWRVEYPFVVLTPDTEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPLTPM 240 Query: 241 SAVINTEKLEQLGAVEMEILPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSAE 300 SAVINTEKLEQLGAVEMEILPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSAE Sbjct: 241 SAVINTEKLEQLGAVEMEILPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSAE 300 Query: 301 ASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEVARF 360 ASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEVARF Sbjct: 301 ASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEVARF 360 Query: 361 KLEWSHPGEKGQKTEVFKTEILEISGKKFRKEGLGKDVTDKFLSGLPGVQKEGCDGLITS 420 KLEWSHPGEKGQKTEVFKTEILEISGKKFRKEGLGKDVTDKFLSGLPGVQKEGCDGLITS Sbjct: 361 KLEWSHPGEKGQKTEVFKTEILEISGKKFRKEGLGKDVTDKFLSGLPGVQKEGCDGLITS 420 Query: 421 ARWILHKMPKQTRTVCLEFFGQARDAIPSIVEIKDYLDAETKKGGAILAGLEHLDERYLR 480 ARWILHKMPKQTRTVCLEFFGQARDAIPSIVEIKDYLDAETKKGGAILAGLEHLDERYLR Sbjct: 421 ARWILHKMPKQTRTVCLEFFGQARDAIPSIVEIKDYLDAETKKGGAILAGLEHLDERYLR 480 Query: 481 AVGYATKSKRGVLPKMVLIGDIVGDDENAVAAAASEVIRMANNRVGEGFVAVSPEARKKF 540 AVGYATKSKRGVLPKMVLIGDIVGDDENAVAAAASEVIRMANNRVGEGFVAVSPEARKKF Sbjct: 481 AVGYATKSKRGVLPKMVLIGDIVGDDENAVAAAASEVIRMANNRVGEGFVAVSPEARKKF 540 Query: 541 WLDRSRTAAIAKHTNAFKINEDVVIPLNRMGEYTDGIERINIELSIKNKLQLLAELDSFF 600 WLDRSRTAAIAKHTNAFKINEDVVIPLNRMGEYTDGIERINIELSIKNKLQLLAELDSFF Sbjct: 541 WLDRSRTAAIAKHTNAFKINEDVVIPLNRMGEYTDGIERINIELSIKNKLQLLAELDSFF 600 Query: 601 VKGNLPLGKSDDAEGDDIPAAEMLEDRVHQAESLLEQTHARWSYLLANLDKPLGEAKGEL 660 VKGNLPLGKSDDAEGDDIPAAEMLEDRVHQAESLLEQTHARWSYLLANLDKPLGEAKGEL Sbjct: 601 VKGNLPLGKSDDAEGDDIPAAEMLEDRVHQAESLLEQTHARWSYLLANLDKPLGEAKGEL 660 Query: 661 AALGLEKMLPVFEQRLVDQPEAAVFHVVQDRTVRISWKQEVRAQLRQIFSGAAFKLILEE 720 AALGLEKMLPVFEQRLVDQPEAAVFHVVQDRTVRISWKQEVRAQLRQIFSGAAFKLILEE Sbjct: 661 AALGLEKMLPVFEQRLVDQPEAAVFHVVQDRTVRISWKQEVRAQLRQIFSGAAFKLILEE 720 Query: 721 CQAIHKRVLRGRVFVALHMHAGDGNVHTNIPVNSDHYEMLQDAHVAVARIMKLARSLNGV 780 CQAIHKRVLRGRVFVALHMHAGDGNVHTNIPVNSDHYEMLQDAHVAVARIMKLARSLNGV Sbjct: 721 CQAIHKRVLRGRVFVALHMHAGDGNVHTNIPVNSDHYEMLQDAHVAVARIMKLARSLNGV 780 Query: 781 ISGEHGIGITKLEFLTEDEIGEFRAYKKRVDPEGRFNKGKLLNLPGMEADLSNAYTPSFG 840 ISGEHGIGITKLEFLTEDEIGEFR YKKRVDPEGRFNKGKLLNLPGMEADLSNAYTPSFG Sbjct: 781 ISGEHGIGITKLEFLTEDEIGEFREYKKRVDPEGRFNKGKLLNLPGMEADLSNAYTPSFG 840 Query: 841 LMGHESLIMQQSDIGAIASSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILATSLLVEA 900 LMGHESLIMQQSDIGAIASSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILATSLLVEA Sbjct: 841 LMGHESLIMQQSDIGAIASSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILATSLLVEA 900 Query: 901 FLYEEQTRRGVSIKHWEEFEDVADHCTVCHKCVTPCPVDIDFGDVSMNMRNLLRKMGKKS 960 FLYEEQTRRGVSIKHWEEFEDVADHCTVCHKCVTPCPVDIDFGDVSMNMRNLLRKMGKKS Sbjct: 901 FLYEEQTRRGVSIKHWEEFEDVADHCTVCHKCVTPCPVDIDFGDVSMNMRNLLRKMGKKS 960 Query: 961 FNAGTNAAMFFLNATDPATINATRKVMTQWGFKAQRLGNDLMKKFAKKQTQKPPATVGKP 1020 FNAGTNAAMFFLNATDPATINATRKVMTQWGFKAQRLGNDLMKKFAKKQTQKPPATVGKP Sbjct: 961 FNAGTNAAMFFLNATDPATINATRKVMTQWGFKAQRLGNDLMKKFAKKQTQKPPATVGKP 1020 Query: 1021 PVKEQVIHFINKKMPGNLPKKTARALLDIEDDKIVPIIRNPKTTTADTEAVFYFPGCGSE 1080 PVKEQVIHFINKKMPGNLPKKTARALLDIEDDKIVPIIRNPKTTTADTEAVFYFPGCGSE Sbjct: 1021 PVKEQVIHFINKKMPGNLPKKTARALLDIEDDKIVPIIRNPKTTTADTEAVFYFPGCGSE 1080 Query: 1081 RLFSQVGLATQAMLWNVGVQTVLPPGYLCCGYPQRGTGDFEKGEKIITDNRVLFHRMANT 1140 RLFSQVGLATQAMLWNVGVQTVLPPGYLCCGYPQRGTGDFEKGEKIITDNRVLFHRMANT Sbjct: 1081 RLFSQVGLATQAMLWNVGVQTVLPPGYLCCGYPQRGTGDFEKGEKIITDNRVLFHRMANT 1140 Query: 1141 LNYLDIKTVVVSCGTCYDQLQGYEFEKIFPGCRIIDIHEYLLEKGVKLEGVTGTRYMYHD 1200 LNYLDIKTVVVSCGTCYDQLQGYEFEKIFPGCRIIDIHEYLLEKGVKLEGVTGTRYMYHD Sbjct: 1141 LNYLDIKTVVVSCGTCYDQLQGYEFEKIFPGCRIIDIHEYLLEKGVKLEGVTGTRYMYHD 1200 Query: 1201 PCHSPMKQQDPLKTVNSLITTVDAQKIEKNDRCCGESGTFGVSRPDVSTQVRFRKEEEMR 1260 PCHSPMKQQDPLKTVNSLITT+DAQKIEKNDRCCGESGTFGVSRPDVSTQVRFRKEEEMR Sbjct: 1201 PCHSPMKQQDPLKTVNSLITTIDAQKIEKNDRCCGESGTFGVSRPDVSTQVRFRKEEEMR 1260 Query: 1261 KGSDKVRADGFTGDVKILTSCPSCFQGLSRYNEDAGTTADYIVVEMARHLLGENWMPEYV 1320 KGSDKVRADGFTGDVKILTSCPSCFQGLSRYNEDAGTTADYIVVEMARHLLGENWMPEYV Sbjct: 1261 KGSDKVRADGFTGDVKILTSCPSCFQGLSRYNEDAGTTADYIVVEMARHLLGENWMPEYV 1320 Query: 1321 ERANNGGIERILV 1333 ERANNGGIERILV Sbjct: 1321 ERANNGGIERILV 1333 >gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked subunit) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 92.0 bits (227), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 97/392 (24%), Positives = 154/392 (39%), Gaps = 76/392 (19%) Query: 193 EYPFVVLTPDTEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPLTPLSAVINTEKLEQL 252 + P VV+ P+ E ++ ++K C L + I+PRG GTG +GGA P+ V++T K Q+ Sbjct: 50 QLPMVVVLPENEAQVVAIMKTCHALQVQIVPRGAGTGLSGGATPIAD-GVVVSTAKFNQI 108 Query: 253 GAVEMEILPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSAEASCIGGNVAMNA 312 ++M DK T GV +S+AA G +A DP+S A IGGNVA N+ Sbjct: 109 --LQM------DKYSRTAVVQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNVAENS 160 Query: 313 GGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEVARFKLEWSHPGEKGQ 372 GG + +G + N+ RMV +G Sbjct: 161 GGVHCLKYGLTVHNVLRVRMVTIEG----------------------------------- 185 Query: 373 KTEVFKTEILEISGKKFRKEGLGKDVTDKFLSGLPGVQKEGCDGLITSARWILHKMPKQT 432 E++E+ GL D+ F+ EG G++T L P+ Sbjct: 186 -------EVVELGSGALDAPGL--DLLAVFIG------SEGMLGVVTEVTVKLVPKPQAA 230 Query: 433 RTVCLEFFGQARDAIPSIVEIKDYLDAETKKGGAILAGLEHLDERYLRAVGYATKSKRGV 492 R + F D + + + + A G I AGLE +D+ R V K+ + Sbjct: 231 RVIMASF----DDVVKGGNAVANVIAA-----GIIPAGLEMMDKTSSRMVEPFVKAGYDI 281 Query: 493 LPKMVLIGDIVGDDENAVAAAASEVIRMANNRVGEGFVAV----SPEARKKFWLDRSRTA 548 + +L+ + + V E+ RM + G A+ S R +FW R Sbjct: 282 DAEAILL----CESDGTVEEVEEEIGRMTDVLNASGATAIACSQSEAERLRFWSGRKNAF 337 Query: 549 AIAKHTNAFKINEDVVIPLNRMGEYTDGIERI 580 A + D IP ++ + GI + Sbjct: 338 PAAGRISPDYYCMDGTIPRKKLAQVLLGIAEM 369 >gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 83.6 bits (205), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 103/396 (26%), Positives = 156/396 (39%), Gaps = 75/396 (18%) Query: 180 RVSHVTDATDWRVEYPFVVLTPDTEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPLTP 239 R H D + + P V+ + +E+A VK C + IIP G GT G + L Sbjct: 37 REHHGRDESSYDPMLPDAVVFAHSTEEVAAFVKLCSQYDTPIIPYGAGTSLEGHVLALQG 96 Query: 240 LSAVINTEKLEQLGAVEMEILPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSA 299 ++ ++ Q+ AV E L T AGV K+++ + G F +DP A Sbjct: 97 -GVTVDLSQMNQVLAVHAEDL--------TATVQAGVTRKQLNQEIKDTGLFFPIDP-GA 146 Query: 300 EASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEVAR 359 +AS +GG + A G AV +GT +N + +V QG Sbjct: 147 DAS-LGGMASTRASGTNAVRYGTMKENTLTLTVVTAQG---------------------- 183 Query: 360 FKLEWSHPGEKGQKTEVFKTEILEISGKKFRKEGLGKDVTDKFLSGLPGVQKEGCDGLIT 419 E+ KT G + +K G D+T + V EG G+IT Sbjct: 184 ---------------EIIKT------GTRAKKSSAGYDLTRVY------VGSEGTLGIIT 216 Query: 420 SARWILHKMPKQTRTVCLEFFGQARDAIPSIVEIKDYLDAETKKGGAILAGLEHLDERYL 479 L+ P+ F A DA+ +++ +T + G LA +E LDE + Sbjct: 217 EVTVRLYPQPEAISAAICSFPTVA-DAVNTVI--------QTIQMGVPLARVELLDENGV 267 Query: 480 RAVGYATKSKRGVLPKMVLIGDIVGDDENAVAAAASEVIRMANNRVGEGFV-AVSPEARK 538 RA+ K V P +L+ + G EN V A V +A GF A PE R Sbjct: 268 RAINAHDKLSLPVNP--LLLFEFHG-SENGVKEQAELVQDIAKEFHALGFEWATRPEDRT 324 Query: 539 KFWLDRSRT--AAIAKHTNAFKINEDVVIPLNRMGE 572 + W R A + A I+ D +P++R+ E Sbjct: 325 RLWTARHNAYFALLQLRPGARAISTDCCVPISRLAE 360 >gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 75.1 bits (183), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 94/397 (23%), Positives = 152/397 (38%), Gaps = 78/397 (19%) Query: 188 TDWRVEY---PFVVLTPDTEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPLTPLSAVI 244 TD R Y VL P E+A +V+ C + + ++P+GG TG G++P +AV+ Sbjct: 36 TDQRGRYTGRALAVLRPADSAEVAAVVRLCAQFAVPLVPQGGNTGLVLGSVPDQQGNAVV 95 Query: 245 -NTEKLEQLGAVEMEILPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSAEASC 303 + +L ++ AV D TI +G V + + + A AG +F + +AE SC Sbjct: 96 LSLRRLNRIRAV--------DALNNTITVESGCVLQHLQEQAAAAGRLFPLS-LAAEGSC 146 Query: 304 -IGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEVARFKL 362 IGGN++ NAGG + +G + +V QG+ + Sbjct: 147 TIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQGEIMS--------------------- 185 Query: 363 EWSHPGEKGQKTEVFKTEILEISGKKFRKEGLGKDVTDKFLSGLPGVQKEGCDGLITSAR 422 S K RK+ G D+ D F+ EG G+IT+A Sbjct: 186 ----------------------SLKGLRKDNTGYDLRDLFIGA------EGTLGVITAAV 217 Query: 423 WILHKMPKQTRTVCLEFFGQARDAIPSIVEIKDYLDAETKKGGAILAGLEHLDERYLRAV 482 L P+ T A A+P + L G L G E + + L+ V Sbjct: 218 MKLFPQPRAQVT--------ALAALPHPAQALQLLQLAQAHCGPALTGFELMSDFCLQLV 269 Query: 483 GYATKSKRGVL----PKMVLIGDIVGDDENAVAAAASEVIRMA--NNRVGEGFVAVSPEA 536 +R P+ VL+ + E A +I A V + +A S Sbjct: 270 RKHFPEQRLPFDRPHPQYVLLELSDSESEEHARALFEGLIGTALEQGLVDDAVIAASLAQ 329 Query: 537 RKKFWLDRSRTAAIAKHTNAFKINEDVVIPLNRMGEY 573 + W R ++ H I D+ +P++R+ E+ Sbjct: 330 SRALWRLRESISSAQAHEGK-NIKHDISVPISRIAEF 365 >gi|300313119|ref|YP_003777211.1| glycolate oxidase iron-sulfur subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 414 Score = 65.1 bits (157), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 90/439 (20%), Positives = 159/439 (36%), Gaps = 82/439 (18%) Query: 859 SSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILATSLLVEAFLYEEQTRRGVSIKHWEE 918 S ++ C+ CG C C T+ + L PR +I ++E T+ + Sbjct: 20 SILRSCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGKPATAATQSHL------- 72 Query: 919 FEDVADHCTVCHKCVTPCPVDIDFGDVSMNMRNLLRKMGKKSFNAGTNAAMFFLNATDPA 978 D C C C + CP + +G + Sbjct: 73 -----DRCLTCRNCESTCPSGVQYGRL--------------------------------- 94 Query: 979 TINATRKVMTQWGFK--AQRLGNDLMKKFAKKQTQKPPATVGKPPVKEQVIHFINKKMPG 1036 ++ RKV+ + + +QR+ +K+ ++ PA ++ + + K+P Sbjct: 95 -VDIGRKVVEEQVPRPLSQRVMRTALKELVPRKWIFRPAMKAGQMLRPLLPKLLQNKVP- 152 Query: 1037 NLPKKTARALLDIEDDKIVPIIRNPKTTTADTEAVFYFPGCGSERLFSQVGLATQAMLWN 1096 LP++ T + Y GC + + AT +L Sbjct: 153 -LPQEAGAW-----------------PTRTHARQMLYLEGCVQPSMSPNINSATARVLDA 194 Query: 1097 VGVQTVLPPGYLCCGYPQRGTGDFEKGEKIITDNRVLFHRMANTLNYLDIKTVVVSCGTC 1156 +GVQ PP CCG + D E G + + N + + + T+V++ C Sbjct: 195 LGVQLFAPPKAGCCGAIRYHMNDQEGGLEDMRRNIDAWWPYIEGRDGIRADTIVMNASGC 254 Query: 1157 YDQLQGY----EFEKIFP-GCRII-----DIHEYL--LEKGVK--LEGVTGTRYMYHDPC 1202 ++ Y + + ++ R I DI E L E+ +K L+ G R YH PC Sbjct: 255 GSTVKEYGHLLQHDPVYAEKARRISHMTRDISEILPEFEEALKHKLKDFNGKRVAYHPPC 314 Query: 1203 HSPMKQQDPLKTVNSL-ITTVDAQKIEKNDRCCGESGTFGVSRPDVSTQVRFRKEEEMRK 1261 Q+ K L VD Q + CCG +GT+ + +P++S ++R K +++ Sbjct: 315 TLQHGQKIRGKVEGILRAAGVDVQLCADSHLCCGSAGTYSILQPELSYRLRDNKISKLQA 374 Query: 1262 GSDKVRADGFTGDVKILTS 1280 + G G V L S Sbjct: 375 TDPDMIVTGNIGCVTHLQS 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,083,221 Number of extensions: 171687 Number of successful extensions: 509 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 504 Number of HSP's successfully gapped: 5 Length of query: 1333 Length of database: 1,619,870 Length adjustment: 97 Effective length of query: 1236 Effective length of database: 1,160,575 Effective search space: 1434470700 Effective search space used: 1434470700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 136 (57.0 bits)