BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0015.p (498 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 991 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 346 2e-96 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 326 3e-90 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 251 7e-68 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 124 1e-29 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 991 bits (2563), Expect = 0.0, Method: Compositional matrix adjust. Identities = 495/498 (99%), Positives = 496/498 (99%) Query: 1 MPAFRMEHPESRGENNVATNDNSLTDARVRMLPYIVAATFFMEYLDTTIIATALPQMAQS 60 MPAFRMEHPESRGENNVATNDNSLTDARVRMLPYIVAATFFMEYLDTTIIATALPQMAQS Sbjct: 1 MPAFRMEHPESRGENNVATNDNSLTDARVRMLPYIVAATFFMEYLDTTIIATALPQMAQS 60 Query: 61 FGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVW 120 FGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVW Sbjct: 61 FGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVW 120 Query: 121 EFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITT 180 EFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITT Sbjct: 121 EFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITT 180 Query: 181 YASWHWIFLMNVPFGLAAFTAVMMVVRNEHGVSKRPLDLVGFLLSGAALTSLLYGTEIAS 240 YASWHWIFLMNVPFGLAAFTAVMMVVRNEHGVSKRPLDLVGFLLSGAALTSLLYGTEIAS Sbjct: 181 YASWHWIFLMNVPFGLAAFTAVMMVVRNEHGVSKRPLDLVGFLLSGAALTSLLYGTEIAS 240 Query: 241 HMESNIKVALGFVALGVVLGYGAWRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVD 300 HMESNIKVALGFVALGVVLGYGAWRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVD Sbjct: 241 HMESNIKVALGFVALGVVLGYGAWRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVD 300 Query: 301 AVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAG 360 AVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAG Sbjct: 301 AVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAG 360 Query: 361 GLIIACGLLTPATPLPVILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQ 420 GLIIACGLLTPATPLPVILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQ Sbjct: 361 GLIIACGLLTPATPLPVILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQ 420 Query: 421 QMTIGMGIAFGALALRIAAALRGEEIDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLA 480 QMTIGMGIAFGALALRIAAALRGE IDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLA Sbjct: 421 QMTIGMGIAFGALALRIAAALRGEAIDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLA 480 Query: 481 RDAGSNIGGGVRAAQGKR 498 RDAGSNIGGG+RAA GKR Sbjct: 481 RDAGSNIGGGMRAAPGKR 498 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 346 bits (888), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 187/445 (42%), Positives = 271/445 (60%), Gaps = 6/445 (1%) Query: 45 LDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIA 104 +D+T+I+T+LP +A V P L L +T+Y+++LAVFIPISGW+ADRFGS T+F AI Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 105 IFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPA 164 +F L SILCG S + +F AAR LQG+GGAMMVPVGR++++RS K L+KA++ +T PA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 165 IVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEHGVSKRPLDLVGFLL 224 + PV+GP +GGFITTY W WIF +N+P + + + PLDL GFLL Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 225 SGAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHMQRIEHPLLDFSNLRIPT 284 S + L+ G A + +VAL V G+V H +R PL+D + L+IPT Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 285 YSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQ 344 V GS R+G+ A+P+L+PLM Q+GFG T F SG+L A+A G + MK R L+ Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 345 RFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCYGLSRSMQFSTLATLAYADI 404 R+GFR V + N A+ + A TP TP +++ ++ G RS+QF++L ++YAD+ Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 405 SDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEEIDASGAHFTLGDFQWAFAM 464 R S A+++ SVAQQ++IGMG+ AL+++ ++G A DF AF + Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGH------ATLVAQDFWPAFLV 414 Query: 465 AGVVTLISVIGYRGLARDAGSNIGG 489 G V +++ R L DAG+ + G Sbjct: 415 VGAVAACALLMLRQLPHDAGAEMAG 439 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 326 bits (835), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 184/451 (40%), Positives = 275/451 (60%), Gaps = 16/451 (3%) Query: 45 LDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIA 104 +D T++AT+LP +A+ P L L MTAY+ L VFIPISGW+AD+ G+R VF A+ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 105 IFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPA 164 +F SILC S ++ F AAR LQG+GGAMMVPVGR+I+ RS K L+KA++ +T PA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 165 IVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEHGVSKRPLDLVGFLL 224 ++ PVVGP +GGFITTY W W+FL+N+PF ++N + PLD+ GF+L Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 225 S--GAALTSL---LYGTEIASHMESNIKVALGFVALGVVLGYGAWRHMQRIEHPLLDFSN 279 + GAA+ L L+G+ + ++G +AL + +RH +IE PLLDF Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMY-----YRHSTQIEFPLLDFRL 235 Query: 280 LRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFT 339 LRIPT +V+ GSL RIG+ AVP+L+PL Q G G+TAFQ+G + A+A G+L ++ Sbjct: 236 LRIPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLA 295 Query: 340 TRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCYGLSRSMQFSTLATL 399 R+L+R+GFR+V + NA AG + A G+ + TP +I+ ++ G+ ++QF+ L ++ Sbjct: 296 GRVLRRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSI 355 Query: 400 AYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEEIDASGAHFTLGDFQ 459 AYADI ++L SV QQ+++G+G+ + L+++ L+G A DF Sbjct: 356 AYADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGH------ATIVEADFW 409 Query: 460 WAFAMAGVVTLISVIGYRGLARDAGSNIGGG 490 AF + G+ ++ SV L +AG + G Sbjct: 410 PAFLVIGLFSVASVPISGKLPHNAGLELSRG 440 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 251 bits (642), Expect = 7e-68, Method: Compositional matrix adjust. Identities = 160/452 (35%), Positives = 251/452 (55%), Gaps = 13/452 (2%) Query: 30 RMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWV 89 R L +IVA+ FFM+ LDTTI+ TALP MA+S G + + AY LT+A P SGW+ Sbjct: 52 RALLWIVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWL 111 Query: 90 ADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTG 149 ADRFG+R ++ AI +F+L S+LC + ++ AARV+QGLGG+M++PVGR+ V+R+ Sbjct: 112 ADRFGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVP 171 Query: 150 KDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNE 209 + + A+A+I+ + PVVGP +GG++ SWHWIFL+N+P G+ AV+ + ++ Sbjct: 172 GAQYIAALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDD 231 Query: 210 HGVSKRPLDLVGFLLSGAALT--SLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHM 267 DL+G +L A + SL SH + +AL VA VLGY WRH Sbjct: 232 SLAGTPRFDLLGCVLLSACMVAFSLALDVPFDSH-RTGWSIALFVVAALTVLGY--WRHA 288 Query: 268 QRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLA 327 PL P +S+ + + RIG AVP+L+PL+ Q+ G + +G++LL Sbjct: 289 SVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLP 348 Query: 328 TALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCYGL 387 A+ K + +++RFG+ + N AI G I++ L+TP PL + + +G Sbjct: 349 AAIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGA 408 Query: 388 SRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEEID 447 S SMQF+ + ++ + S ++L+S+ Q + IG+G+ G + + + G + Sbjct: 409 SNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTATN 468 Query: 448 ASGAHFTLGDFQWAFAMAGVVTLISVIGYRGL 479 AS FQ FA+ G +TL S +R + Sbjct: 469 AS--------FQITFAIVGAITLASAWVFRKM 492 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 124 bits (312), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 90/294 (30%), Positives = 151/294 (51%), Gaps = 6/294 (2%) Query: 35 IVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFG 94 ++AA F+ LDTTI ++ +A + G ++ + +T+Y + A+ +P++GW+A+RFG Sbjct: 37 LLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFG 96 Query: 95 SRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLM 154 + VF +++ +F L S LCG + ++ A RVLQGL G ++P+ + +++R K++ Sbjct: 97 TVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAP 156 Query: 155 KAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGL-AAFTAVMMVVRNEHGVS 213 A+ +VAP+ GP +GG + SW +IF +NVP L + M+ R E +S Sbjct: 157 AAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMS 216 Query: 214 KRPLDLVGFLLS---GAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHMQRI 270 K P+D VG +L AAL +L + S V L A+ + + W +R Sbjct: 217 KLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTER- 275 Query: 271 EHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLL 324 +P++D R ++ +V T L L PL Q G TA SG + Sbjct: 276 -NPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCI 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,314,954 Number of extensions: 49052 Number of successful extensions: 338 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 323 Number of HSP's successfully gapped: 19 Length of query: 498 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 409 Effective length of database: 1,198,455 Effective search space: 490168095 Effective search space used: 490168095 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)