BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0013.p (257 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 502 e-144 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 159 2e-40 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 126 2e-30 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 125 4e-30 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 121 5e-29 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 502 bits (1293), Expect = e-144, Method: Compositional matrix adjust. Identities = 255/257 (99%), Positives = 256/257 (99%) Query: 1 MSKQDQQAAVSPSTLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVS 60 MSKQDQQAAVS STLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVS Sbjct: 1 MSKQDQQAAVSTSTLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVS 60 Query: 61 SVQAKGGRALAIQADSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLD 120 SVQAKGGRALAIQADSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLD Sbjct: 61 SVQAKGGRALAIQADSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLD 120 Query: 121 RTLAVNVRSVFVATQEAARYMGEGGRIISIGSTNADRIPFAGGAVYAMSKSALVGLTKGL 180 RTLAVNVRSVFVATQEAARYMGEGGR+ISIGSTNADRIPFAGGAVYAMSKSALVGLTKGL Sbjct: 121 RTLAVNVRSVFVATQEAARYMGEGGRVISIGSTNADRIPFAGGAVYAMSKSALVGLTKGL 180 Query: 181 ARDLGPRGITVNNVQPGPVDTDMNPASGDFAASLTGLMALPRYGKAEEIASFVAYLAGPE 240 ARDLGPRGITVNNVQPGPVDTDMNPASGDFAASLTGLMALPRYGKAEEIASFVAYLAGPE Sbjct: 181 ARDLGPRGITVNNVQPGPVDTDMNPASGDFAASLTGLMALPRYGKAEEIASFVAYLAGPE 240 Query: 241 SGYITGANLMIDGGFSA 257 SGYITGANLMIDGGFSA Sbjct: 241 SGYITGANLMIDGGFSA 257 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 159 bits (402), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 105/245 (42%), Positives = 142/245 (57%), Gaps = 6/245 (2%) Query: 17 SKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQADS 76 ++VA V GG+ GIGA+I Q+LAA+G TV ++ D A ++ A G RA +Q D Sbjct: 8 ARVALVTGGAMGIGASISQQLAAKGHTVLVADINL-DAASKTADALVAAGHRAAPLQMDL 66 Query: 77 ADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRSVFVATQE 136 ++ A + +GR D+LVNNAGV + LE+ +T+ VNV V +A Q Sbjct: 67 GSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQH 126 Query: 137 AARYM--GEGGRIISIGSTNADRIPFAGGAVYAMSKSALVGLTKGLARDLGPRGITVNNV 194 AAR M + GRI++I S + R AG Y SK+A++GLT+ +A +L GITVN+V Sbjct: 127 AARLMVKQQWGRIVNISSISGIR-AGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSV 185 Query: 195 QPGPVDTDMNPA--SGDFAASLTGLMALPRYGKAEEIASFVAYLAGPESGYITGANLMID 252 PGPVDT M A S S L+ + RYG +EIAS V YLA ES YITG + +D Sbjct: 186 APGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVD 245 Query: 253 GGFSA 257 GGF A Sbjct: 246 GGFVA 250 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 126 bits (316), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 77/244 (31%), Positives = 129/244 (52%), Gaps = 4/244 (1%) Query: 15 LVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQA 74 + ++A+V GG GIG AI RL +G TV + + +++++ +G A + Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 Query: 75 DSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRSVFVAT 134 + ++ + + A G +D+LVNNAG+ G + +D D + N+ S+F T Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 Query: 135 QEAARYMGEG--GRIISIGSTNADRIPFAGGAVYAMSKSALVGLTKGLARDLGPRGITVN 192 ++ M E GRII+I S N + F G Y+ +K+ + G T LA+++ +G+TVN Sbjct: 121 KQVIEGMCERGFGRIINISSVNGQKGQF-GQTNYSTAKAGIHGFTMALAQEVATKGVTVN 179 Query: 193 NVQPGPVDTDMNPA-SGDFAASLTGLMALPRYGKAEEIASFVAYLAGPESGYITGANLMI 251 V PG + TDM A + + + + R G+ EEIAS VA++A E GY TG++ + Sbjct: 180 TVSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSL 239 Query: 252 DGGF 255 +GG Sbjct: 240 NGGL 243 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 125 bits (313), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 100/257 (38%), Positives = 143/257 (55%), Gaps = 20/257 (7%) Query: 10 VSPSTLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKG--- 66 +S L +VA V G SRGIG AI LA +GA V T +S A A+ + A G Sbjct: 1 MSAQNLQGQVALVTGASRGIGRAIATELARQGAKVIGT-ATSESGAAAITEYLAALGPEA 59 Query: 67 GRALAIQADSADAA-ALQQAIRLAAETFGRLDILVNNAGV----LAMGPLDQFKLEDLDR 121 GR + + A+A+ AL + ++ + FG L ILVNNAG+ LAM + K E+ D Sbjct: 60 GRGAVLNVNDAEASVALVEQVQ---KDFGSLSILVNNAGITQDQLAM----RMKDEEWDS 112 Query: 122 TLAVNVRSVFVATQEAARYM--GEGGRIISIGSTNADRIPFAGGAVYAMSKSALVGLTKG 179 +A N+ +V ++ R M GRII+I S + G YA +K+ + G+++ Sbjct: 113 VIATNLTAVGRLSRAVLRGMMKARTGRIINITSVVGE-AGNPGQMNYAAAKAGVAGMSRA 171 Query: 180 LARDLGPRGITVNNVQPGPVDTDMNPASGD-FAASLTGLMALPRYGKAEEIASFVAYLAG 238 LAR++G R ITVN V PG +DTDM A + +A++ + L R G AEEIA VA+LA Sbjct: 172 LAREIGSRNITVNCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLAS 231 Query: 239 PESGYITGANLMIDGGF 255 P++ YITG L ++GG Sbjct: 232 PQAAYITGTTLNVNGGM 248 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 121 bits (304), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 90/250 (36%), Positives = 127/250 (50%), Gaps = 9/250 (3%) Query: 13 STLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAI 72 +TL KVA + G S GIG A A GA + T D + + + + A+GGRA + Sbjct: 2 NTLQGKVALITGASAGIGRATALLFARHGAALILT-ARRQDALEQVAALICAQGGRASVV 60 Query: 73 QADSADAAALQQAIRLAAETFGRLDILVNNAGVL-AMGPLDQFKLEDLDRTLAVNVRSVF 131 D +AA ++ + +A + FG LDI +NNAG + A PL + + TL N+ S F Sbjct: 61 AGDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAF 120 Query: 132 VA--TQEAARYMGEGGRIISIGSTNADRIPFAGGAVYAMSKSALVGLTKGLARDLGPRGI 189 + Q A G I+ S G A Y +K+ L+GL KG+A D +GI Sbjct: 121 LGARCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGI 180 Query: 190 TVNNVQPGPVDTDM--NPASGDFAASLTGLMALPRYGKAEEIASFVAYLAGPESGYITGA 247 N + PG VDTDM +P +AA GL A+ R + EEIA+ +LA P + ++TG Sbjct: 181 RANALLPGGVDTDMGGDPQQKQWAA---GLHAMKRIAQPEEIAAAALFLASPMASFVTGT 237 Query: 248 NLMIDGGFSA 257 L DGG SA Sbjct: 238 ALYADGGNSA 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 729,804 Number of extensions: 28311 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 51 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 51 Length of query: 257 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 174 Effective length of database: 1,226,865 Effective search space: 213474510 Effective search space used: 213474510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)