BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0007.p (226 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313180|ref|YP_003777272.1| phosphoglycolate phosphatase [H... 455 e-129 gi|300312992|ref|YP_003777084.1| phosphoglycolate phosphatase [H... 85 4e-18 gi|300313787|ref|YP_003777879.1| HAD-superfamily hydrolase [Herb... 64 1e-11 gi|300311212|ref|YP_003775304.1| phosphoglycolate phosphatase [H... 59 3e-10 >gi|300313180|ref|YP_003777272.1| phosphoglycolate phosphatase [Herbaspirillum seropedicae SmR1] Length = 226 Score = 455 bits (1170), Expect = e-129, Method: Compositional matrix adjust. Identities = 223/226 (98%), Positives = 224/226 (99%) Query: 1 VSKLTNIKAAIIDLDGTMLDTAADFHVAVNRMRDDLGLTPLSQETIVNFVGKGTENLIRR 60 +SKLTNIKAAIIDLDGTMLDTAADFHVAVNRMRDDLGLTPLSQETIVNFVGKGTENLIRR Sbjct: 1 MSKLTNIKAAIIDLDGTMLDTAADFHVAVNRMRDDLGLTPLSQETIVNFVGKGTENLIRR 60 Query: 61 VLAVDYAEDEAAQYFQQALAAYTEHYLAINGDYSSLYPGVLEGLQALRNKGLRLACVTNK 120 VLAVDYAEDEAAQYFQQALAA TEHYLAINGDYSSLYPGVLEGLQALRNKGLRLACVTNK Sbjct: 61 VLAVDYAEDEAAQYFQQALAACTEHYLAINGDYSSLYPGVLEGLQALRNKGLRLACVTNK 120 Query: 121 PLAFAVPLLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCQDFALEPAQVVAIGDSSND 180 PLAFAVPLLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCQDFAL PAQVVAIGDSSND Sbjct: 121 PLAFAVPLLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCQDFALAPAQVVAIGDSSND 180 Query: 181 AQAARAAGCRVLNVPYGYNHGESIHDVDSDGIVSTLVEAAQQISVD 226 AQAARAAGCRVLNVPYGYNHGESIHDVDSDGIVSTLVEAAQQISVD Sbjct: 181 AQAARAAGCRVLNVPYGYNHGESIHDVDSDGIVSTLVEAAQQISVD 226 >gi|300312992|ref|YP_003777084.1| phosphoglycolate phosphatase [Herbaspirillum seropedicae SmR1] Length = 218 Score = 85.1 bits (209), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 62/191 (32%), Positives = 86/191 (45%), Gaps = 5/191 (2%) Query: 8 KAAIIDLDGTMLDTAADFHVAVNRMRDDLGLTPLSQETIVNFVGKGTENLIRRVLAVDYA 67 +A + DLDGT+ DTA D A N +R GL E + G LI L + Sbjct: 7 RAILFDLDGTLADTAPDLAAAANFLRQQRGLEDAPYEQLRPVASAGARGLIGAALGIRPG 66 Query: 68 EDEAAQYFQQALAAYTEHYLAINGDYSSLYPGVLEGLQALRNKGLRLACVTNKPLAFAVP 127 EDE +++ + + Y A +S L+ G+ E L L +G+ VTNK F Sbjct: 67 EDE----YEELRVRFLDRYQANMTTHSRLFDGIPELLVQLEARGIAWGIVTNKAARFTDV 122 Query: 128 LLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCQDFALEPAQVVAIGDSSNDAQAARAA 187 L + GL IV GD+ P KP P PLL+ + L P +GD D QA +AA Sbjct: 123 LAPQIGLGHAGCIV-SGDTTPHSKPHPAPLLEAARRLQLTPEDCWYVGDDLRDIQAGKAA 181 Query: 188 GCRVLNVPYGY 198 + +GY Sbjct: 182 AMPTIAAAWGY 192 >gi|300313787|ref|YP_003777879.1| HAD-superfamily hydrolase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 63.9 bits (154), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 59/206 (28%), Positives = 82/206 (39%), Gaps = 35/206 (16%) Query: 4 LTNIKAAIIDLDGTMLDTAADFHVAVNRMRDDLGLTPLSQETIVNFVGK---GTENLIRR 60 L I + DLDGT++D+ A H A N + L + V + G N Sbjct: 59 LMPITHFLFDLDGTLMDSDALHHAAFNTI-----LARWDRHIDVEYYKTHIMGASN---- 109 Query: 61 VLAVDYAEDEAAQYFQQALAAY-TEHYLAINGDYSSLY-----------PGVLEGLQALR 108 A F E YL + + SL+ PG+ L+ +R Sbjct: 110 -----------AMIFGHLFPGMPAEQYLPLAEEKESLFRSQLDQRVEPTPGIERLLEHIR 158 Query: 109 NKGLRLACVTNKPLAFAVPLLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCQDFALEP 168 G R A VTN P A A +L+ GL F+ + GD + KPDP+P L Q E Sbjct: 159 RIGGRSAVVTNAPRANAELMLKATGLAGQFDTLVIGDELARAKPDPLPYLTGLQLLGGEA 218 Query: 169 AQVVAIGDSSNDAQAARAAGCRVLNV 194 VA DSS+ +AA +AG + Sbjct: 219 GAAVAFEDSSSGVKAASSAGIWTFGM 244 >gi|300311212|ref|YP_003775304.1| phosphoglycolate phosphatase [Herbaspirillum seropedicae SmR1] Length = 219 Score = 59.3 bits (142), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 49/188 (26%), Positives = 87/188 (46%), Gaps = 9/188 (4%) Query: 11 IIDLDGTMLDTAADFHVAVNRMRDDLGLTPLSQETIVNFV-GKGTENLIRRVLAVDYAED 69 + D DGT++D+ + + DLGL P+ ++ ++V G G ++ ++ + D Sbjct: 10 VFDWDGTLMDSTSTIVRCIQAAARDLGL-PIPDKSAASYVIGLGLQDAMQAAIP-----D 63 Query: 70 EAAQYFQQALAAYTEHYLAINGDYSSLYPGVLEGLQALRNKGLRLACVTNKPLAFAVPLL 129 +Y+ + + Y HYL + D + L+ GV E L L +G LA T K + Sbjct: 64 VDPKYYPRIVERYRHHYLGQDKDLT-LFEGVPEMLADLSQQGYFLAVATGKSRVGLNRAM 122 Query: 130 QKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCQDFALEPAQVVAIGDSSNDAQAARAAGC 189 GL F+ D KP P L ++ ++ + + + IGD+++D Q A AG Sbjct: 123 NTTGLLSMFDASRCADE-TFSKPHPAMLQELTRELGQDMHRTLMIGDTTHDLQMAINAGA 181 Query: 190 RVLNVPYG 197 + V +G Sbjct: 182 AGVAVEFG 189 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 650,304 Number of extensions: 24542 Number of successful extensions: 87 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 4 Length of query: 226 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 144 Effective length of database: 1,231,600 Effective search space: 177350400 Effective search space used: 177350400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)