BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0001.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 525 e-150 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 181 5e-47 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 154 5e-39 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 139 2e-34 gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 111 6e-26 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 525 bits (1353), Expect = e-150, Method: Compositional matrix adjust. Identities = 257/258 (99%), Positives = 258/258 (100%) Query: 1 MSYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKA 60 MSYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKA Sbjct: 1 MSYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKA 60 Query: 61 FAAGADISAMAGYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFI 120 FAAGADISAMAGYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFI Sbjct: 61 FAAGADISAMAGYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFI 120 Query: 121 IAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRI 180 IAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRI Sbjct: 121 IAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRI 180 Query: 181 VAADKLLEEAMAAALIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATEDQ 240 VAADKLLEEAMAAA+IIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATEDQ Sbjct: 181 VAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATEDQ 240 Query: 241 KEGMKAFLEKRQPVFKHS 258 KEGMKAFLEKRQPVFKHS Sbjct: 241 KEGMKAFLEKRQPVFKHS 258 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 181 bits (459), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 104/256 (40%), Positives = 154/256 (60%), Gaps = 2/256 (0%) Query: 1 MSYENIQVETH-EKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEK 59 ++YE++ H E V + L+RP NAL + + E+ AL + + + ++I+G+EK Sbjct: 5 VAYEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEK 64 Query: 60 AFAAGADISAMAGYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDF 119 AFAAGAD++ M D + + + + W + R KP++AAV GYALG GCEL M D Sbjct: 65 AFAAGADLNEMIHKDAIAT-QLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADI 123 Query: 120 IIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSR 179 IAA AK GQPEI +G +PGAGGTQRL R V K AM + L+G + A++A +AGLV+ Sbjct: 124 AIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAE 183 Query: 180 IVAADKLLEEAMAAALIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATED 239 +V D LE +A A IA+ + K+++ +++E L G+ +ER F A+ D Sbjct: 184 VVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASAD 243 Query: 240 QKEGMKAFLEKRQPVF 255 ++EG+ AF EKR VF Sbjct: 244 RQEGIAAFQEKRAAVF 259 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 154 bits (390), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 96/262 (36%), Positives = 145/262 (55%), Gaps = 9/262 (3%) Query: 1 MSYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKA 60 MS +I +E + + +I L+RP LN+ + + EL +A+ A + +++TG+ + Sbjct: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 Query: 61 FAAGADISAMA---GYDYMDVFKGEFITRNW----ETLRRIRKPVIAAVAGYALGGGCEL 113 F AG D+ A G D +D+ GE I + + ++LR + PVI AV G A G G L Sbjct: 61 FCAGQDLGDRAVKPGDDGVDL--GESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANL 118 Query: 114 AMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAER 173 + CD ++AA +A F + KLG++P GGT LPR V A+AM +AL G + AE+AER Sbjct: 119 PLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAER 178 Query: 174 AGLVSRIVAADKLLEEAMAAALIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYS 233 GL+ + V D+L+EEA A A A P + K ++ + TL E + ER + Sbjct: 179 WGLIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRA 238 Query: 234 CFATEDQKEGMKAFLEKRQPVF 255 + D +EG+ AFLEKR P F Sbjct: 239 LGRSRDYREGVTAFLEKRAPQF 260 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 139 bits (350), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 90/258 (34%), Positives = 133/258 (51%), Gaps = 3/258 (1%) Query: 2 SYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITG-SEKA 60 +Y N+++E ++ L P A N + + +L + +A +I +++TG EK Sbjct: 3 TYTNLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKF 61 Query: 61 FAAGADISAMAGYDY-MDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDF 119 F+AGAD+ A D M +ETL R IAA+ GYA+GGG E A+ CD Sbjct: 62 FSAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDI 121 Query: 120 IIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSR 179 IA + A+ PE +G++P AGGTQ LP V + A + L G ++AE A R GLV Sbjct: 122 RIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEE 181 Query: 180 IVAADKLLEEAMAAALIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATED 239 +V + E A+A A + K + A L ++ ER LF F T+D Sbjct: 182 VVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQD 241 Query: 240 QKEGMKAFLEKRQPVFKH 257 Q+EG++AFLEKR P +K+ Sbjct: 242 QREGVQAFLEKRSPQWKN 259 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 111 bits (277), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 80/260 (30%), Positives = 124/260 (47%), Gaps = 16/260 (6%) Query: 5 NIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAAG 64 +IQ+ + V I R NA++ + +L +AL ++ + I+ TG KAF+AG Sbjct: 4 DIQLSKADGVLTIHFDREDKKNAITAAMYQQLADALKEAESDSAVRAILFTGKPKAFSAG 63 Query: 65 ADISAMAGYDYMDVFKGEFITRN-------WETLRRIRKPVIAAVAGYALGGGCELAMMC 117 D+ D K T++ W+ + KP++AAV+G A+G G L + C Sbjct: 64 NDLE--------DFLKNPPKTQDAPVFQFLWQ-ISHASKPIVAAVSGVAVGIGTTLLLHC 114 Query: 118 DFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLV 177 D + AADNAKF P +LG+ P A + LP +A + L G A+EA + G V Sbjct: 115 DLVYAADNAKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGFV 174 Query: 178 SRIVAADKLLEEAMAAALIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFAT 237 +R++A ++LL A A +A +P + K + + T + M E F Sbjct: 175 NRVMAQEELLPYAQQQAAKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTA 234 Query: 238 EDQKEGMKAFLEKRQPVFKH 257 + KE AF EKR+P FK Sbjct: 235 PEAKEAFTAFFEKRKPDFKQ 254 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 716,101 Number of extensions: 27626 Number of successful extensions: 112 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 103 Number of HSP's successfully gapped: 11 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)