BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0053.p (556 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 1136 0.0 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 273 3e-74 gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 181 2e-46 gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 167 2e-42 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 120 3e-28 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 1136 bits (2939), Expect = 0.0, Method: Compositional matrix adjust. Identities = 552/556 (99%), Positives = 555/556 (99%) Query: 1 MPELIPAPRQPDYADVYAGFRIETLRKQFQGDFETGINACVECCDRHVEGDNIALDFVSL 60 MPELIPAPRQPDYADVYAGFRIETLR+QFQGDFETGINACVECCDRHV+GDNIAL+FVSL Sbjct: 1 MPELIPAPRQPDYADVYAGFRIETLREQFQGDFETGINACVECCDRHVKGDNIALEFVSL 60 Query: 61 HGEHQQYSFAQVRSMAARVANLLRQQGIQPGQIVAGMLPRTPELLATVLGTLRAGAVYQP 120 HGEHQQYSFAQVRSMAARVANLLRQQGIQPGQIVAGMLPRTPELLATVLGTLRAGAVYQP Sbjct: 61 HGEHQQYSFAQVRSMAARVANLLRQQGIQPGQIVAGMLPRTPELLATVLGTLRAGAVYQP 120 Query: 121 LFTAFGPKAIEHRLALSGASLIVTNVANRDKLDEIANCPQVCTVRESNDPLRAGDIDFRA 180 LFTAFGPKAIEHRLALSGASLIVTNVANRDKLDEIANCPQVCTVRESNDPLRAGDIDFRA Sbjct: 121 LFTAFGPKAIEHRLALSGASLIVTNVANRDKLDEIANCPQVCTVRESNDPLRAGDIDFRA 180 Query: 181 AVDAQSDDFAPVLRSGADLMLMMSTSGTTGAAKGVPVPLSALQAFSVYMREAVGLLPQDK 240 AVDAQSDDFAPVLRSGADLMLMMSTSGTTGAAKGVPVPLSALQAFSVYMREAVGLLPQDK Sbjct: 181 AVDAQSDDFAPVLRSGADLMLMMSTSGTTGAAKGVPVPLSALQAFSVYMREAVGLLPQDK 240 Query: 241 FWNMADPGWAYGLYYAIIGPLAIGQGITFNEAAFTVSGTYETIRRLGITSLAGAPTAYRM 300 FWNMADPGWAYGLYYAIIGPLAIG GITFNEAAFTVSGTYETIRRLGITSLAGAPTAYRM Sbjct: 241 FWNMADPGWAYGLYYAIIGPLAIGHGITFNEAAFTVSGTYETIRRLGITSLAGAPTAYRM 300 Query: 301 MMAAGEEAAASVKGRLRAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGMVVNNHHA 360 MMAAGEEAAASVKGRLRAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGMVVNNHHA Sbjct: 301 MMAAGEEAAASVKGRLRAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGMVVNNHHA 360 Query: 361 LRHEVVPGSAGFAMPGYRVVVLDENHQELGANQPGELAIDIARSPLYWFSGYWKQDTPAI 420 LRHEVVPGSAGFAMPGYRVVVLDENHQELGANQPGELAIDIARSPLYWFSGYWKQDTPAI Sbjct: 361 LRHEVVPGSAGFAMPGYRVVVLDENHQELGANQPGELAIDIARSPLYWFSGYWKQDTPAI 420 Query: 421 AHGYYSTGDNVELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIGLP 480 AHGYYSTGDNVELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIGLP Sbjct: 421 AHGYYSTGDNVELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIGLP 480 Query: 481 DPERTEIVKAFVVLSDQFKASDALREQLAQHVKRRLSAHAYPRAIEFLDALPKTPSGKLQ 540 DPERTEIVKAFVVLSDQFKASDALREQLAQHVKRRLSAHAYPRAIEFLDALPKTPSGKLQ Sbjct: 481 DPERTEIVKAFVVLSDQFKASDALREQLAQHVKRRLSAHAYPRAIEFLDALPKTPSGKLQ 540 Query: 541 RFVLRKMEADRRAGHP 556 RFVLRKMEADRRAGHP Sbjct: 541 RFVLRKMEADRRAGHP 556 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 273 bits (698), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 190/535 (35%), Positives = 274/535 (51%), Gaps = 25/535 (4%) Query: 29 FQGDFETGINACVECCDRHVEGDN-IALDFVSLHGEHQQYSFAQVRSMAARVANLLRQQG 87 ++ T N CDR +G A+ G YS+ Q++ ++ R AN LR G Sbjct: 24 YEWQIPTHFNIAQAACDRWADGSGRTAIICEDADGSVTSYSYDQLKILSDRFANALRAAG 83 Query: 88 IQPGQIVAGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVTNVA 147 + G + L + E + T L + GA+ PLF FGP AI +RL SGA +VT+ + Sbjct: 84 VGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTDAS 143 Query: 148 NRDKLDEIANCP--QVCTVRESNDPLRAGDIDFRAAVDAQSDDFAPVLRSGADLMLMMST 205 +K P ++ ES+D + DF + A + PVL D +++ T Sbjct: 144 GMEKATLAGELPALRLVFCVESSDLVLPQTTDFWDRLHAAPAELDPVLTLADDPAMIIYT 203 Query: 206 SGTTGAAKGVPVPLSALQAFSVYMREAVGL------LPQ--DKFWNMADPGWAYGLYYAI 257 SGTTG AKG AL A V + G+ PQ D+FW AD W GL + Sbjct: 204 SGTTGKAKG------ALHAHRVLLGHLPGVEVSHDSFPQEGDRFWTPADWAWIGGLLDVL 257 Query: 258 IGPLAIGQGITFNE-AAFTVSGTYETIRRLGITSLAGAPTAYRMMMAAGEEAAASVKGRL 316 + L G + F + + + R I ++ PTA +M+ A A L Sbjct: 258 LPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPPTALKMLRGAAT-VRAQADFSL 316 Query: 317 RAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGMVVNNHHALRHEVVPGSAGFAMPG 376 R+V+S GE L +++ W AL V I++ YGQTE +VV++ + V GS G A+PG Sbjct: 317 RSVASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVSSSSHC-YPSVSGSMGRAVPG 375 Query: 377 YRVVVLDENHQELGANQPGELAIDIARSPLYWFSGYWKQDTPA---IAHGYYSTGDNVEL 433 + V ++DE Q L G +AI A P+ F YW+ + A + TGD + Sbjct: 376 HVVQIVDEQGQVLPHGTVGNIAIR-APDPVM-FLRYWRNEEATREKFAGDFLLTGDLGSM 433 Query: 434 EPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIGLPDPERTEIVKAFVV 493 + G I ++GR+DDVITSAGYRIGP +E L+ HPAV AV+G+ D RTE+VKAFVV Sbjct: 434 DEQGYIRYLGRNDDVITSAGYRIGPAAIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVV 493 Query: 494 LSDQFKASDALREQLAQHVKRRLSAHAYPRAIEFLDALPKTPSGKLQRFVLRKME 548 L D +AL+ +L QHV+ +L+AH YPR I F+ ALP T +GK+ R L++M+ Sbjct: 494 LKDGVTPDEALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLKEMD 548 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 181 bits (458), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 155/556 (27%), Positives = 244/556 (43%), Gaps = 51/556 (9%) Query: 37 INACVECCDRHVEG---DNIALDFVSLHGEHQQYSFAQVRSMAARVANLLRQQGIQPGQI 93 +N C D ++E D A+ F S G+ + S+ ++ + AN L+ GI+ G Sbjct: 81 VNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELHQKVCQFANGLKSLGIKKGDR 140 Query: 94 VAGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVT--------- 144 V +P + E +A + R GA + +F F K++ R+ +GA ++T Sbjct: 141 VVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVLTADYQVRGGK 200 Query: 145 NVANRDKLDEIANCPQVCTVRESNDPLRAG---------DIDFRAAVDAQSDDFAPVLRS 195 + + +DE + T++ RAG D+ V QSD P Sbjct: 201 QLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRDLWLSDVVANQSDTCEPEWVD 260 Query: 196 GADLMLMMSTSGTTGAAKGVPVPLSALQAFSVY-MREAVGLLPQDKFWNMADPGWAYGLY 254 + ++ TSG+TG KGV + ++V M+ + P D +W AD GW G Sbjct: 261 AEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHT 320 Query: 255 YAIIGPLAIGQGITFNEAAFTVSGT---YETIRRLGITSLAGAPTAYRMMMAAGEEAAAS 311 Y GPLA+G E T ++ + R T APTA R ++ A + A Sbjct: 321 YIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAAD-ADEK 379 Query: 312 VKGR------LRAVSSAGEPLNAEVVRWFASAL---DVPIHDHYGQTELGMVVNNHHALR 362 V + LR + S GEP+N E W+ + + PI D + QTE G + + Sbjct: 380 VHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIVDTFWQTETGGHMISPLPGA 439 Query: 363 HEVVPGSAGFAMPGYRVVVLDENHQELGANQPGELAID---------IARSPLYWFSGYW 413 VPGS +PG ++DE ++ G L + I P + Y+ Sbjct: 440 TPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPWPSMIRTIWNDPERFKKSYF 499 Query: 414 KQDTPAIAHGYYSTGDN-VELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVA 472 ++ + Y GD V + G + GR DDV+ +G+R+G ++ESAL+ + VA Sbjct: 500 PEE---LGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVA 556 Query: 473 DVAVIGLPDPERTEIVKAFVVLSDQFKASDALRE---QLAQHVKRRLSAHAYPRAIEFLD 529 + AV+G PD E + AFVVL D ++ +L V + + A P+ I F D Sbjct: 557 EAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGD 616 Query: 530 ALPKTPSGKLQRFVLR 545 LPKT SGK+ R +LR Sbjct: 617 NLPKTRSGKIMRRLLR 632 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 167 bits (424), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 154/562 (27%), Positives = 250/562 (44%), Gaps = 50/562 (8%) Query: 38 NACVECCDRHV--EGDNIALDFVSLHGEHQQ-YSFAQVRSMAARVANLLRQQGIQPGQIV 94 N C DRH+ AL +S ++ Y++A+++ +A ++ G+Q G V Sbjct: 52 NLCHNAVDRHLPERAQQAALIAISTETNSERVYTYAELQQEVMAMAASMQALGVQRGDRV 111 Query: 95 AGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVTNVAN------ 148 +P E + +L R GAV+ +F F ++ R+ + LIV+ A Sbjct: 112 LIYMPMIAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKV 171 Query: 149 ---RDKLDEIANCPQ-----VCTVRESNDPLRAG---DIDFRAAVDAQSDDFAPV--LRS 195 + LDE Q V V P+ D+D+ PV L S Sbjct: 172 IPYKGLLDEAIAMAQHRPQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQQVPVTWLES 231 Query: 196 GADLMLMMSTSGTTGAAKGVPVPLSALQ-AFSVYMREAVGLLPQDKFWNMADPGWAYGLY 254 A ++ TSGTTG KGV + A + M+ P + ++ +D GW G Sbjct: 232 NASSYILY-TSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHS 290 Query: 255 YAIIGPLAIGQGITFNEAAFTVSGT---YETIRRLGITSLAGAPTAYRMMMAAGEEAAAS 311 Y + GPL G E + + + +T + APTA R++ E Sbjct: 291 YIVYGPLIAGMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECMER 350 Query: 312 V-KGRLRAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGM-VVNNHHALRHEVVP-G 368 L+A+ AGEPL+ W + AL VP+ D+Y QTE G +++ + + G Sbjct: 351 YDTSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLG 410 Query: 369 SAGFAMPGYRVVVLDENHQE-LGANQPGELAIDIARSP-----LY-----WFSGYWKQDT 417 S G MPGYR+ +LDE E G +Q G +AI+ P +Y + + YW+ Sbjct: 411 SPGVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYWR--- 467 Query: 418 PAIAHGYYSTGDNVELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVI 477 ++ YST D + +G +GR+DDVI AG+R+G ++E ++ HP V++VAV+ Sbjct: 468 -SLPREVYSTFDWGIRDKDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVV 526 Query: 478 GLPDPERTEIVKAFVVLSDQF-----KASDALREQLAQHVKRRLSAHAYPRAIEFLDALP 532 G+ D + ++ AF + + + AL ++ V +++ A P + F+ LP Sbjct: 527 GVEDKLKGQVAFAFAIPKQAYTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLP 586 Query: 533 KTPSGKLQRFVLRKMEADRRAG 554 KT SGKL R ++ + R G Sbjct: 587 KTRSGKLLRRAIQSICEGRDPG 608 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 120 bits (301), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 138/536 (25%), Positives = 220/536 (41%), Gaps = 68/536 (12%) Query: 61 HGEHQQY-------SFAQVRSMAARVANLLRQQGIQPGQIVAGMLPRTPELLATVLGTLR 113 H + Q Y S+ ++ S++ + L+ G+Q G VA M+P + + LR Sbjct: 36 HADRQAYVCMGKYLSYRELDSLSRALGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILR 95 Query: 114 AGAVY---QPLFTAFGPKAIEHRLALSGASLIV---------------TNVAN------R 149 AG PL+TA + +EH+L SGA I TN+ + Sbjct: 96 AGYTVVNVNPLYTA---RELEHQLKDSGAEAIFVLENFACTLQKVVAHTNIKHIVVASMG 152 Query: 150 DKLDEIANCPQVCTVRESNDPLRA----GDIDFRAAVD-AQSDDFAPVLRSGADLMLMMS 204 + L + VR + A I F A+ A+ PV + D+ + Sbjct: 153 ELLGALKGTLVNLVVRHVKKLVPAWSLPNAITFSQALSQARGKTLQPVTLTHDDIAFLQY 212 Query: 205 TSGTTGAAKGVPVPLSALQAFSVYMREAVGLLPQDKFWNMADP---GWAYGLYYAIIGPL 261 T GTTG AKG + L A + E L P K D A LY+ I L Sbjct: 213 TGGTTGVAKGAVLTHRNLVANVLQAEE--WLTPALKAGKPIDQLVFVCALPLYH--IFAL 268 Query: 262 AIGQGITFNEAAFT--------VSGTYETIRRLGITSLAGAPTAYRMMMAAGEEAAASVK 313 + + E A + G + + + + + T Y ++ + A Sbjct: 269 TVCNMLGTREGALNLLIPNPRDIPGFVKELAKYKVNTFPAVNTLYNALLNNADFARLDFS 328 Query: 314 GRLRAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGMVVNNHHALRHEVVPGSAGFA 373 G R G + V + PI + YG +E + + + E G+ G Sbjct: 329 G-YRCCVGGGMAVQKSVADKWKKLTGCPIIEGYGLSETSPIASANPCTIDEYT-GTIGLP 386 Query: 374 MPGYRVVVLDENHQELGANQPGELAIDIARSPLYWFSGYWKQ--DTPAIA--HGYYSTGD 429 +P +LD++ Q + QPGE+AI R P GYW++ +T + G++ TGD Sbjct: 387 LPSTEFAILDDDGQPVPLGQPGEIAI---RGPQV-MPGYWQRPDETAKVMTPDGFFKTGD 442 Query: 430 NVELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIGLPDPERTEIVK 489 ++ G + R D+I +G+ + P +VE + +HP V + A +G+PD E VK Sbjct: 443 IGVMDERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEAVK 502 Query: 490 AFVVLSDQFKASDALREQLAQHVKRRLSAHAYPRAIEFLDALPKTPSGKLQRFVLR 545 FVV D+ ++ QL + K + + + P+ IEF D LPKT GK+ R LR Sbjct: 503 LFVVRKDKTLSA----AQLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 554 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,839,252 Number of extensions: 81511 Number of successful extensions: 338 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 300 Number of HSP's successfully gapped: 16 Length of query: 556 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 466 Effective length of database: 1,193,720 Effective search space: 556273520 Effective search space used: 556273520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)