BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0050.p (382 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 789 0.0 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 277 1e-75 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 244 1e-65 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 160 1e-40 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 140 1e-34 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 789 bits (2038), Expect = 0.0, Method: Compositional matrix adjust. Identities = 382/382 (100%), Positives = 382/382 (100%) Query: 1 MLLSPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGA 60 MLLSPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGA Sbjct: 1 MLLSPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGA 60 Query: 61 GMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGC 120 GMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGC Sbjct: 61 GMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGC 120 Query: 121 FCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGIS 180 FCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGIS Sbjct: 121 FCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGIS 180 Query: 181 CFLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAG 240 CFLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAG Sbjct: 181 CFLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAG 240 Query: 241 RIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRA 300 RIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRA Sbjct: 241 RIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRA 300 Query: 301 AQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIY 360 AQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIY Sbjct: 301 AQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIY 360 Query: 361 EGANDIQRLVIGRAIASDQAHA 382 EGANDIQRLVIGRAIASDQAHA Sbjct: 361 EGANDIQRLVIGRAIASDQAHA 382 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 277 bits (709), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 150/374 (40%), Positives = 225/374 (60%), Gaps = 3/374 (0%) Query: 3 LSPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGM 62 L+ + + + R FA + PFAA WD FP + + + ELG G+ PE GG G+ Sbjct: 5 LNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGL 64 Query: 63 DYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFC 122 + + EE+A +T+ +++ N ++ I +G P+ K QW + LA G +G +C Sbjct: 65 SRLDATVVFEELARACTSTAAYLTIHNMVSWMIA-SWGQPSLKAQWCEQLAAGRKIGSYC 123 Query: 123 LTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCF 182 LTEP +GSDAA+++T A + ++LNG+K FI+ V +V A T G +G+S Sbjct: 124 LTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTG-GEGARGVSAI 182 Query: 183 LVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRI 242 +VP + PG G+ E KMG ++ T I DN +VPA LLG+EG+G+ A+ L+ GRI Sbjct: 183 VVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRI 242 Query: 243 GIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQ 302 IA SVG A+AA +AA +Y ++R+ FG PI + QA+ F+LADM T L AAR MV AA Sbjct: 243 NIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAAT 302 Query: 303 LKDQGRP-CLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYE 361 D G P +MAK A++ ++ ++A+Q+HGG GY ++P+ER +RDVR+ QI E Sbjct: 303 KLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILE 362 Query: 362 GANDIQRLVIGRAI 375 G N+I R++I R + Sbjct: 363 GTNEIMRVIISRKL 376 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 244 bits (622), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 125/375 (33%), Positives = 221/375 (58%), Gaps = 6/375 (1%) Query: 10 IRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVL 69 +R+A+ FA + P AA+ DR+ FP + K+L +LG +G+ V E++GGAG+ Y++ ++ Sbjct: 18 LREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGYLAHII 77 Query: 70 ALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTG 129 A+EEI+ + ++L ++ G+ QK ++L L G+ +G ++EP+ G Sbjct: 78 AMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAG 137 Query: 130 SDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCFLVPCDTP 189 SD +++ RA++ GD ++LNG+K +IT+G A V +V+A TD AG +G++ FLV Sbjct: 138 SDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARGMTAFLVEKGYK 197 Query: 190 GFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSV 249 GF V + +K+G S T +++ +C VP +LG G G + +S L+ R ++ + Sbjct: 198 GFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPL 257 Query: 250 GMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQL------ 303 G+ +A + V Y R+ FG I E Q + +LADM + + A + V+ Q Sbjct: 258 GIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADS 317 Query: 304 KDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEGA 363 D+ R K+A+ A ++++E A +A +AIQ GG GY +++PV R++RD ++ +I G Sbjct: 318 ADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYPVGRLWRDAKLYEIGAGT 377 Query: 364 NDIQRLVIGRAIASD 378 ++I+R++IGR + +D Sbjct: 378 SEIRRMLIGRELFAD 392 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 160 bits (406), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 122/373 (32%), Positives = 193/373 (51%), Gaps = 31/373 (8%) Query: 4 SPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMD 63 S H +RDA+R + + D +P + + L + G + +P+++GG+G+ Sbjct: 7 SDSHADLRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLG 66 Query: 64 YMSLVLALEEIAAGDGATSTIVSVQNSLAC-------GITEKYGSPTQKEQWLKPLARGE 116 + +EEI G NS AC G ++GSP QKE++L +A GE Sbjct: 67 LTEASVIMEEINRTGG---------NSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGE 117 Query: 117 M-LGCFCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVT----D 171 + L +TEP TG+D I+T A + GD +++NG K +I+ +H+ + I+ A T Sbjct: 118 LRLQSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQ 177 Query: 172 RSAGKKGISCFLVPCDTPGFVVGRSEE-----KMGQHASDTVQIMLDNCRVPATALLGKE 226 S +G+S FLV +G E M H +T ++ DN +PA L+G+E Sbjct: 178 VSRKSEGMSIFLVDLKE---AIGHGMEVRPILNMVNH--ETNELFFDNLEIPAENLIGEE 232 Query: 227 GEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADM 286 G G+R L L A R IAA+ +G + A YAR+R F PI +Q V F LA+ Sbjct: 233 GRGFRYILDGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEA 292 Query: 287 NTLLDAARLMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFP 346 ++AA LM ++A L D + C +A+MAK A++AA + A+ +Q HGG G+ ++ Sbjct: 293 YIEVEAANLMRYQACALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYD 352 Query: 347 VERIYRDVRICQI 359 VER +R+ R+ Q+ Sbjct: 353 VERKFRETRLYQV 365 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 140 bits (354), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 94/340 (27%), Positives = 173/340 (50%), Gaps = 18/340 (5%) Query: 41 KELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYG 100 + +A+ G + +PE +GG + + LA EE+ A D + + + L +G Sbjct: 51 QAMADAGFIKALLPESFGGTALSTLDFALAAEELTAVDVNVPSAL-LGTGLGLHAVVHHG 109 Query: 101 SPTQKEQWLKPLAR-GEMLGCFCLTEPHTGSD------AAAIRTRAERDGDDFILNGTKQ 153 +P QK+++L G L TE G++ + ++T A +GD++++NG K Sbjct: 110 TPEQKQRFLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKH 169 Query: 154 FITSG-----KHAGVAIVFAVTDRS-AGKKGISCFLVPCDTPGFVVGRSEEKMGQHASDT 207 + T+G K A + V TD + ++ ++ +VP + PG + + MG A+ + Sbjct: 170 YTTNGTGWDGKGAHLYSVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTMGHRATIS 229 Query: 208 VQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQ-R 266 ++ +N RVPA+ ++GK G+G + + I + VG+ R AF+ A+ +A+ R Sbjct: 230 PRVHFNNVRVPASNIVGKPGDGISMLEAAFSWTAALIGSACVGVMRTAFDIALRFAQSDR 289 Query: 267 ESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQLKD--QGRPCLKEASMAKMFASEA 324 S +P+IEHQ V F LAD+ ++AAR + W++ D +GR + A + K + SE Sbjct: 290 RSGTIPVIEHQNVGFMLADIKMRIEAARYLTWKSCHQLDLTEGR-SRELAIITKTYCSEL 348 Query: 325 AEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEGAN 364 ++ DA+++ G YT +P+ + +D +Y+G N Sbjct: 349 CVQVVYDAMRVVGVDAYTDLYPLAGLMQDALCFPLYDGGN 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,169,146 Number of extensions: 48286 Number of successful extensions: 208 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 13 Length of query: 382 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 295 Effective length of database: 1,207,925 Effective search space: 356337875 Effective search space used: 356337875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)