BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0046.p (413 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protei... 840 0.0 gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herba... 370 e-103 gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47... 127 2e-30 gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirill... 91 1e-19 >gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 840 bits (2169), Expect = 0.0, Method: Compositional matrix adjust. Identities = 411/413 (99%), Positives = 411/413 (99%) Query: 1 MNEPAPEVLLNAQAQRIPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDG 60 MNEPAPEVLLNAQAQRIPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDG Sbjct: 1 MNEPAPEVLLNAQAQRIPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDG 60 Query: 61 DDVRRNTELYRGNDELVEMSNGCICCTLRADLLEQVSALARSDRFDYLLIESTGISEPIP 120 DDVRRNTELYRGNDELVEMSNGCICCTLRADLLEQVSALARS RFDYLLIESTGISEPIP Sbjct: 61 DDVRRNTELYRGNDELVEMSNGCICCTLRADLLEQVSALARSGRFDYLLIESTGISEPIP 120 Query: 121 VAETFAFLDSDGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGASTRPLSDLLIE 180 VAETFAFLDSDGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGASTRPLSDLLIE Sbjct: 121 VAETFAFLDSDGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGASTRPLSDLLIE 180 Query: 181 QVEYANVILVSRVDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTRLFDLPS 240 QVEYANVILVSRVDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTRLFDLPS Sbjct: 181 QVEYANVILVSRVDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTRLFDLPS 240 Query: 241 LARMPGWMQTMDGAHPPSEADTYGISSSVYRARLPFHPGRLLDWLGRPWRNGRLLRCKGY 300 LARMPGWMQTMDGAHPPSEADTYGISSSVYRARLPFHPGRLLDWLGRPWRNGRLLRCKGY Sbjct: 241 LARMPGWMQTMDGAHPPSEADTYGISSSVYRARLPFHPGRLLDWLGRPWRNGRLLRCKGY 300 Query: 301 LWISSRYPDIALLVQTGGQFQWGFVGRWWRFIEPAQWPQDTYRRQGIQEKWDALAGDCRQ 360 LWISSRY DIALLVQTGGQFQWGFVGRWWRFIEPAQWPQDTYRRQGIQEKWDALAGDCRQ Sbjct: 301 LWISSRYSDIALLVQTGGQFQWGFVGRWWRFIEPAQWPQDTYRRQGIQEKWDALAGDCRQ 360 Query: 361 ELVFIGQGIDGDLLTEELDACLLTTTEIEAGPDAWAQLYGAAAFDERASTTLH 413 ELVFIGQGIDGDLLTEELDACLLTTTEIEAGPDAWAQLYGAAAFDERASTTLH Sbjct: 361 ELVFIGQGIDGDLLTEELDACLLTTTEIEAGPDAWAQLYGAAAFDERASTTLH 413 >gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 370 bits (950), Expect = e-103, Method: Compositional matrix adjust. Identities = 196/393 (49%), Positives = 252/393 (64%), Gaps = 8/393 (2%) Query: 11 NAQAQRIPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDGDDVRRN-TEL 69 A +R+PVTVLSGFLG+GKTTLLNHIL NR G +VAVIVNDMSEVN+D VR +L Sbjct: 7 QAADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADL 66 Query: 70 YRGNDELVEMSNGCICCTLRADLLEQVSALARSDRFDYLLIESTGISEPIPVAETFAFLD 129 R ++LVEMSNGCICCTLR DLL +V LAR RFD L+IESTGISEP+PVAETF F D Sbjct: 67 ARTEEKLVEMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAETFTFTD 126 Query: 130 SDGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGA----STRPLSDLLIEQVEYA 185 DG SLS++ARLDT+VTVV+ F + + ++ Q + R + DLLIEQ+E+ Sbjct: 127 EDGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLIEQIEFC 186 Query: 186 NVILVSRVDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTRLFDLPSLARMP 245 +VI+++++DL+ A L A+L KLNP ARI+ G+V L+QVLDT LFD ++ P Sbjct: 187 DVIVLNKLDLVAADDLERLHAILHKLNPRARIVRAEFGKVALEQVLDTGLFDFEQASQAP 246 Query: 246 GWMQTMDGAHPPSEADTYGISSSVYRARLPFHPGRLLDWLGRPWRNGRLLRCKGYLWISS 305 W+Q + G H P E + YGISS ++AR P HP R D + W ++R KG+ W++S Sbjct: 247 SWLQELRGEHVP-ETEQYGISSFAWQARRPLHPQRFFDLINTEWPG--VVRSKGFFWLAS 303 Query: 306 RYPDIALLVQTGGQFQWGFVGRWWRFIEPAQWPQDTYRRQGIQEKWDALAGDCRQELVFI 365 R Q G + G G+WW + QWPQD I WD GD RQELV I Sbjct: 304 RPTQAGSWSQAGAVCRHGLAGKWWAAVPREQWPQDQESVDFILAHWDENVGDARQELVLI 363 Query: 366 GQGIDGDLLTEELDACLLTTTEIEAGPDAWAQL 398 G +D + L LDACLL+ E+ GP WA++ Sbjct: 364 GMAMDQEALRARLDACLLSDAEMAQGPLGWARM 396 >gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 127 bits (318), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 81/237 (34%), Positives = 132/237 (55%), Gaps = 34/237 (14%) Query: 17 IPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDGDDVRRNTELYRGNDEL 76 IPV++L+GFLGSGKTTLLNH++ VI+N+ E+ +D V + E + + Sbjct: 12 IPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAHSQE-----ETI 66 Query: 77 VEMSNGCICCTLRADLLEQVS------ALARSDRFDYLLIESTGISEPIPVAETFAFLDS 130 VEMS+GC+CCT+R DL + + A +F+ ++IE+TG++ P P+ T L + Sbjct: 67 VEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHT---LMT 123 Query: 131 DGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGASTRPLSDLLIEQVEYANVILV 190 D F ++E RLD ++ V+ +G ST + ++Q A+ +L+ Sbjct: 124 DAF-IAERYRLDGVIVTVDA---------------VNGMSTLDHHEEAVQQAAVADRLLL 167 Query: 191 SRVDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTRLF----DLPSLAR 243 ++ DL+DA+ A+L+A L+ LNP AR L G++ ++LD LF +P +AR Sbjct: 168 TKTDLVDAATLAALQARLRTLNPGARQLQPQVGQIAAAELLDAGLFKPGEKMPDVAR 224 >gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirillum seropedicae SmR1] Length = 377 Score = 91.3 bits (225), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 68/221 (30%), Positives = 123/221 (55%), Gaps = 14/221 (6%) Query: 17 IPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDGDDVRRNTELYRGNDEL 76 IPVTV++GFLG+GKTTLL +L+ R ++A+++N+ EV +DGD +R + +G ++ Sbjct: 17 IPVTVVTGFLGAGKTTLLRGLLQRRQSRRLALLINEFGEVAIDGDLLRAEGD-AQGQVQI 75 Query: 77 VEMSNGCICCTLRADLL-EQVSALARSDRFDYLLIESTGISEPIPVAETFAFLDSDGFSL 135 + ++G I A + ++ AR + D++LIE++G++ P V E G + Sbjct: 76 QDFAHGLIAYGDDAHFVPAMLALAARRAQIDHVLIETSGLALPSAVMEVL-----QGAEM 130 Query: 136 SELARLDTLVTVVNGETFEQQLADHVAIAQADGASTRPLSDLLIEQVEYANVILVSRVDL 195 + LD + VV+ L D A A A GA+T + L +Q+E A+V++++++D Sbjct: 131 ARHFVLDATLAVVDTPLL---LEDAFASADAVGAAT---AQLFRQQLECADVVVLNKIDG 184 Query: 196 IDASAFASLKAVLQKLNPTARILPMAHG-EVDLDQVLDTRL 235 ++ + + ++ L P R L +A+G +D+ L RL Sbjct: 185 MEEAPLLQAEQAVRALAPNVRFLELAYGARLDIRLALGLRL 225 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,370,235 Number of extensions: 60409 Number of successful extensions: 229 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 221 Number of HSP's successfully gapped: 4 Length of query: 413 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 325 Effective length of database: 1,203,190 Effective search space: 391036750 Effective search space used: 391036750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)