BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0044.p (199 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310265|ref|YP_003774357.1| nitrile hydratase subunit alpha... 405 e-114 >gi|300310265|ref|YP_003774357.1| nitrile hydratase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 199 Score = 405 bits (1040), Expect = e-114, Method: Compositional matrix adjust. Identities = 199/199 (100%), Positives = 199/199 (100%) Query: 1 MSNPSSSAQTSTPGERAWALFKVLNDKKLIPEGYIEGLTDLLANKFDPANGARVVAKAWS 60 MSNPSSSAQTSTPGERAWALFKVLNDKKLIPEGYIEGLTDLLANKFDPANGARVVAKAWS Sbjct: 1 MSNPSSSAQTSTPGERAWALFKVLNDKKLIPEGYIEGLTDLLANKFDPANGARVVAKAWS 60 Query: 61 DPQYRELLLRDGTAACEEFGYTGPQGEYIVALEDTPTLKNVIVCSLCSCTNWPVLGLPPE 120 DPQYRELLLRDGTAACEEFGYTGPQGEYIVALEDTPTLKNVIVCSLCSCTNWPVLGLPPE Sbjct: 61 DPQYRELLLRDGTAACEEFGYTGPQGEYIVALEDTPTLKNVIVCSLCSCTNWPVLGLPPE 120 Query: 121 WYKGFEFRARLVREGRTVLAELGTELPKDMTVKVWDTSAETRYLVLPMRPAGTEGWSEEA 180 WYKGFEFRARLVREGRTVLAELGTELPKDMTVKVWDTSAETRYLVLPMRPAGTEGWSEEA Sbjct: 121 WYKGFEFRARLVREGRTVLAELGTELPKDMTVKVWDTSAETRYLVLPMRPAGTEGWSEEA 180 Query: 181 LSKLITKDVLIGVALPKVG 199 LSKLITKDVLIGVALPKVG Sbjct: 181 LSKLITKDVLIGVALPKVG 199 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 686,447 Number of extensions: 28143 Number of successful extensions: 73 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 73 Number of HSP's successfully gapped: 1 Length of query: 199 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 118 Effective length of database: 1,236,335 Effective search space: 145887530 Effective search space used: 145887530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)