BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0043.p (504 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310266|ref|YP_003774358.1| amidase family protein [Herbasp... 1031 0.0 gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 170 3e-43 gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 108 1e-24 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 106 4e-24 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 100 2e-22 >gi|300310266|ref|YP_003774358.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 1031 bits (2666), Expect = 0.0, Method: Compositional matrix adjust. Identities = 502/504 (99%), Positives = 502/504 (99%) Query: 1 MSIVRPTPEQLKELASSLHIQLTTEQASQYLDLMQASFDAYDVIDGLPDEIPAVKYPRSA 60 MSIVRPTPEQLKELASSLHIQLTTEQASQYLDLMQASFDAYDVIDGLPDEIPAVKYPRSA Sbjct: 1 MSIVRPTPEQLKELASSLHIQLTTEQASQYLDLMQASFDAYDVIDGLPDEIPAVKYPRSA 60 Query: 61 AARPAAKDNPLNAWACKSTVKGAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSY 120 AARPAAKDNPLNAWACKSTVKGAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSY Sbjct: 61 AARPAAKDNPLNAWACKSTVKGAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSY 120 Query: 121 DATIVTRLLDAGATILGKATCEHFCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVA 180 DATIVTRLLDAGATILGKATCEHFCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVA Sbjct: 121 DATIVTRLLDAGATILGKATCEHFCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVA 180 Query: 181 AGEADMAIGGDQGGSIRIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDN 240 AGEADMAIGGDQGGSIRIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDN Sbjct: 181 AGEADMAIGGDQGGSIRIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDN 240 Query: 241 ALMLEVIAGADGLDPRQYAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQE 300 ALMLEVIAGADGLDPRQYAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQE Sbjct: 241 ALMLEVIAGADGLDPRQYAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQE 300 Query: 301 AIAQLEKLGASVATVSVEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQH 360 AIAQLEKLGASVATVSVEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQH Sbjct: 301 AIAQLEKLGASVATVSVEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQH 360 Query: 361 HAHWREHADDLSATLKLCMFVGQYGLTRYQGRFYAKAQNIARRARTGYDKALADVDLLVM 420 HAHWREHADDLSATLKLCMFVGQYGLTRYQGRFYAKAQNIARRAR GYDKALAD DLLVM Sbjct: 361 HAHWREHADDLSATLKLCMFVGQYGLTRYQGRFYAKAQNIARRARAGYDKALADFDLLVM 420 Query: 421 PTVPIIAQPLPAAGCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDGLPVGLMLVG 480 PTVPIIAQPLPAAGCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDGLPVGLMLVG Sbjct: 421 PTVPIIAQPLPAAGCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDGLPVGLMLVG 480 Query: 481 KHYAEATLYQAAAAFEASMDWQQH 504 KHYAEATLYQAAAAFEASMDWQQH Sbjct: 481 KHYAEATLYQAAAAFEASMDWQQH 504 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 170 bits (430), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 154/506 (30%), Positives = 235/506 (46%), Gaps = 42/506 (8%) Query: 11 LKELASSLHIQLT--TEQASQYLDLMQAS-FDAYDVIDGLPDEIPAVKYPRSAAARPAAK 67 LKEL++ L + TE A+ +LD + AS +A+ +D + ++ A Sbjct: 6 LKELSAQLQARKISATELATLFLDRIDASDLNAFLHVD------RELTLAQAKVADSRLS 59 Query: 68 DNPLNAWACKSTVKGAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSYDATIVTR 127 DN NA G+L G + KD G GS L+ +V +DAT+V + Sbjct: 60 DN--NA------------GQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQ 105 Query: 128 LLDAGATILGKATCEHFCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVAAGEADMA 187 AG LGK C+ F + G+ S P NP GGSS GSAA VAA A A Sbjct: 106 FNAAGMVTLGKLNCDEFAMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVAARLAPAA 165 Query: 188 IGGDQGGSIRIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVI 247 D GGSIR P++ CG G+K T+G V G++ +++D GPI D L+L + Sbjct: 166 TATDTGGSIRQPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAM 225 Query: 248 AGADGLDPRQYAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLEK 307 G D D + +T +L+ ++GL+IG+ E F G + V V+ A+++ EK Sbjct: 226 TGFDERDSTSLQRPKEDFTRSLNDSLQGLRIGVPKEFFGAG-LAADVEAAVRAALSEFEK 284 Query: 308 LGASVATVSVEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQHHAHWREH 367 LGA + +S+ + L+ ++ I + + +G+ + + D G L + + Sbjct: 285 LGAQLVEISLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAA-DYGNLD-DMYRKSR 342 Query: 368 ADDLSATLKLCMFVGQYGLTR-YQGRFYAKAQNIARRARTGYDKALA----DVDLLVMPT 422 A+ +K + VG Y L+ Y +Y +AQ I R + ALA D+++ P Sbjct: 343 AEGFGEEVKRRILVGSYVLSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPV 402 Query: 423 VPIIAQPL-PAAGCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDG-----LPVGL 476 P +A L P+A Y+A F T + G P MSIPCG G PVGL Sbjct: 403 SPTVAWDLGDKTNDPVANYLADIF-----TLSTSLAGLPGMSIPCGFGQGEKNGKRPVGL 457 Query: 477 MLVGKHYAEATLYQAAAAFEASMDWQ 502 ++G ++ EA L A F+ + DW Sbjct: 458 QIIGNYFDEARLLNVAHQFQQATDWH 483 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 108 bits (270), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 127/415 (30%), Positives = 174/415 (41%), Gaps = 42/415 (10%) Query: 88 LAGRTVVLKDNVALAGVPMMNGSSTLEGF-VPSYDATIVTRLLDAGATILGKATCEHFCL 146 L G V +KD +AG + GS L G V S A +V LL AGA ++GK F Sbjct: 69 LEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFAY 128 Query: 147 SG-GSHTSDPAPVHNPRRHGYGS---GGSSSGSAALVAAGEADMAIGGDQGGSIRIPSAF 202 SG G + P + R+ G GGSSSG+A V G A A+G D GGS+RIPSA Sbjct: 129 SGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSAL 188 Query: 203 CGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVIAGADGLDPRQYAPEV 262 G G K T V GV+P+ A +D +GP+ +V+ A+M V+AG D Sbjct: 189 NGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGED----------- 237 Query: 263 QPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLEKLGASVATVSVEEHAL 322 Y A L +RGL++ + L MD V + A+ +L GA + V Sbjct: 238 --YVAVLAHPLRGLRL-LAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNA 294 Query: 323 AGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQHHAHWREHADDLSATLKLCMFVG 382 A+ G H + N G YD Q D+SA L + Sbjct: 295 LAAINAKGGFTAAEAYAWHRALIAENAAG-YD----QRVVSRILRGKDMSAADLLDV--- 346 Query: 383 QYGLTRYQGRFYAKAQNIARRARTGYDKALADVDLLVMPTVPIIAQPLPAAGCPLAEYVA 442 +A+ + IA+ + LA D LVMPTVPI+A + Y Sbjct: 347 ----------LHARPRWIAQ-----VEAQLAGYDALVMPTVPIVAPRIAELQASDDAYYG 391 Query: 443 RAFEMIGNTAPLDITGHPAMSIPCGTVDGLPVGLMLVGKHYAEATLYQAAAAFEA 497 +M+ N ++ A+S+PC PVGL L + L A EA Sbjct: 392 ANGKMLRNPTLINFLDGCALSLPCHAPGSAPVGLSLAACGGQDKRLLSIGLAVEA 446 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 106 bits (265), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 131/450 (29%), Positives = 190/450 (42%), Gaps = 81/450 (18%) Query: 85 EGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSYDATIVTRLLDAGATILGKATCEHF 144 G L G +++KDN +P G+ L DA V RL +AGA ILGK T Sbjct: 116 RGPLHGIPILVKDNYDTVDMPTTGGTLALATLQAQADAFQVKRLREAGAVILGKTTMHEL 175 Query: 145 CLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVAAGEADMAIGGDQGGSIRIPSAFCG 204 + +S NP GGSS G+ A VAA A +G D GSIRIP+A Sbjct: 176 AAGVTTVSSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAAAGMGSDTCGSIRIPAAHQN 235 Query: 205 TYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVIAGADGLD---------- 254 +G++ T GL +GVMP+ +T D P+ +V D A+ML+ G+D D Sbjct: 236 LFGLRTTRGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDATVGSDPQDSSTVDANGHI 295 Query: 255 PRQYAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLEKLGASVAT 314 P+ Y ++ + ++G +IG+L F D V+ + +A+ QL+ GA V Sbjct: 296 PKSYRDGLRADS------LQGARIGVLRALFGAAPEDAEVSAAINKALQQLKDQGAIVTD 349 Query: 315 VSVEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLG-LLQHH------------ 361 V++ E +GL + G+ + ++ YDLG LQ H Sbjct: 350 VTIPE------------LDGL----LSGSSI-IPYEFKYDLGAYLQSHPGAPVGSLGEIL 392 Query: 362 AHWREHADDLSATLKLCMFVGQYGLTRYQGRFYAKAQNIARRARTGYDKALADV------ 415 A EH + L A L+L V Q + R+ + DV Sbjct: 393 ARGMEH-ELLEAGLRLRNSVD------LQNPKDKEELEKVMLKRSALKSLMTDVMQKNHL 445 Query: 416 DLLVMPTVP----IIAQPLPAA-GCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCG-TV 469 D LV PT+ +I +P A C L+ TG PA+++P G T Sbjct: 446 DTLVYPTIQRKAALIGEPQGGAMNCQLSA----------------TTGLPALALPVGFTE 489 Query: 470 DGLPVGLMLVGKHYAEATLYQAAAAFEASM 499 DGLPV L L+ +AE L A +E + Sbjct: 490 DGLPVSLELLAPEFAEPALLGLAYGWEQKI 519 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 100 bits (250), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 122/422 (28%), Positives = 179/422 (42%), Gaps = 36/422 (8%) Query: 86 GKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSYDATIVTRLLDAGATILGKATCEHFC 145 G L+G + +KDN+ +AG+ G++ L VP AT+V L AGA GKA Sbjct: 64 GPLSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALHAGKANMHELA 123 Query: 146 LSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVAAGEADMAIGGDQGGSIRIPSAFCGT 205 ++ P NP +GGSS G AL+AA A A+G D GGS+RIP+A CG Sbjct: 124 FGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCGV 183 Query: 206 YGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVIAGADGLDPRQYAPEVQPY 265 G++ T GV+PI T D GP+ +V D AL+ +++AG+ P P Q Sbjct: 184 VGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPLLALPPSQ-- 241 Query: 266 TAALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLEKLGASVATVSVE-----EH 320 L++G+L G +++ V A+ QL + G + V + H Sbjct: 242 ----------LRLGVLRNPSWQG-LEEEVEANANAALMQLREAGVELVDVDLPTLAPLSH 290 Query: 321 ALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQHHAHWREHADDLSATLKLCMF 380 A A+ L + + + L ++G + A + ATL + Sbjct: 291 AAGFAIVLHEFLIDLPAYLQASDARDVIKRVLAEVG-------SPDVARIIQATLDQPVP 343 Query: 381 VGQYGLTRYQGRFYAKAQNIARRARTGYDKALADVDLLVMPTVPIIAQPLPAAGCP--LA 438 Y L Q R R++ VD L+ PT + A+PL Sbjct: 344 EHAYRLALEQRRTMQALYRDCFRSQG--------VDALIFPTTALTARPLGEDDTVALRG 395 Query: 439 EYVARAFEMIGNTAPLDITGHPAMSIPCGTVD-GLPVGLMLVGKHYAEATLYQAAAAFEA 497 E V N I G P++SIP G + GLPVGL L G ++ L Q AA+ E Sbjct: 396 ERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASLET 455 Query: 498 SM 499 + Sbjct: 456 VL 457 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,625,272 Number of extensions: 70431 Number of successful extensions: 269 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 11 Length of query: 504 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 415 Effective length of database: 1,198,455 Effective search space: 497358825 Effective search space used: 497358825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)