BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0032.p (431 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 717 0.0 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 424 e-120 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 362 e-101 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 302 4e-83 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 299 3e-82 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 717 bits (1852), Expect = 0.0, Method: Compositional matrix adjust. Identities = 350/358 (97%), Positives = 352/358 (98%), Gaps = 1/358 (0%) Query: 4 HTYCWIDTDQGIDLAGAEADRLPETEEPEAAGSSSSRSELDDALRLYENAKQTTQQMFKQ 63 +TYCWIDTDQGIDLAGAEADRLPETEEPEAAGSSSSRSELDDALRLYENAKQTTQQMFKQ Sbjct: 51 YTYCWIDTDQGIDLAGAEADRLPETEEPEAAGSSSSRSELDDALRLYENAKQTTQQMFKQ 110 Query: 64 ARLGKTLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALART 123 ARLGKTLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALART Sbjct: 111 ARLGKTLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALART 170 Query: 124 LGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSG 183 LGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSG Sbjct: 171 LGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSG 230 Query: 184 VPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAE 243 VPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAE Sbjct: 231 VPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAE 290 Query: 244 SLSRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFV 303 SLSRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFV Sbjct: 291 SLSRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFV 350 Query: 304 TTPSIEVVESEIIDLSAPGQTDAIAERSGQDWLKSNGLVIDSLKAMMAIAGSIPTRAS 361 TTPSIEVVESEIIDLSAPGQTDAIAERSGQDWLKSNGLVIDSLKAMM A +P R S Sbjct: 351 TTPSIEVVESEIIDLSAPGQTDAIAERSGQDWLKSNGLVIDSLKAMME-ASDLPMRIS 407 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 424 bits (1091), Expect = e-120, Method: Compositional matrix adjust. Identities = 207/361 (57%), Positives = 262/361 (72%), Gaps = 13/361 (3%) Query: 6 YCWIDTDQGIDLAGAE-----ADRLPETE---EPEAAGSSSSRSELDDALRLYENAKQTT 57 YCWIDT++G+D+A E A L ETE EPE A + + L DA+ L E AK+ T Sbjct: 51 YCWIDTERGLDVAQEEEAADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALRETAKKAT 110 Query: 58 QQMFKQARLGKTLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLM 117 + MF +AR+GK LD+E C S+VEEIA SV + N LS++RLK DEYTYLHS+AVC LM Sbjct: 111 KAMFSEARMGKVLDMERCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALM 170 Query: 118 VALARTLGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDY 177 VAL RTLG+D+ AC+EAGL G+LHDVGKAFIP E+LNKP +LS++E+ I++HP LGY Y Sbjct: 171 VALGRTLGLDETACREAGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKY 230 Query: 178 LKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKS 237 L VPDY DVCLHHHEKIDG+GYP + I++YSRMAA+CDVYDA+TS+RPYK Sbjct: 231 LSQDPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKR 290 Query: 238 GWDPAESLSRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKP 297 WDPAE++S MASW GHFDR +FLAFVK LGIYP+GSIVKL+SGR+ VIRQN DLT+P Sbjct: 291 AWDPAEAISAMASWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRP 350 Query: 298 VVKAFVTTPSIEVVESEIIDLSAPGQTDAIAERSGQDWLKSNGLVIDSLKAMMAIAGSIP 357 V+K F S+ V ++EI+DL A + D I R G++W + + L AM ++ +P Sbjct: 351 VLKVFHYDDSMTVTDTEILDLLANPEADTIVARGGEEWAR-----LSHLDAMQLLSTGVP 405 Query: 358 T 358 T Sbjct: 406 T 406 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 362 bits (928), Expect = e-101, Method: Compositional matrix adjust. Identities = 176/337 (52%), Positives = 236/337 (70%), Gaps = 5/337 (1%) Query: 6 YCWIDTDQGIDLAGAEADRLPETE-----EPEAAGSSSSRSELDDALRLYENAKQTTQQM 60 YCWID +G D+AGA + + AA + + ++ A+ L E+A++ T+QM Sbjct: 51 YCWIDVSRGRDVAGAAPAKAEAAAAPASPDQSAATHAGADADRASAVALREHAREVTRQM 110 Query: 61 FKQARLGKTLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVAL 120 F AR+GK +++ C +LVEEIA SV RD N LLS++RLK DEYTYLHS++VC L++ L Sbjct: 111 FNAARMGKMVELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGL 170 Query: 121 ARTLGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKG 180 RTLG+ + AC+EAGL+GYLHDVGKAFI EILNKP +L+ +E+ +I+RHP LGY YL Sbjct: 171 GRTLGLSEAACREAGLSGYLHDVGKAFIDDEILNKPGKLTAEEFALIKRHPELGYQYLIQ 230 Query: 181 FSGVPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWD 240 +P Y DVC HHHE++DG GYP I++Y+RM AVCDVYDA+TSERPYK GWD Sbjct: 231 TPDMPAYASDVCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWD 290 Query: 241 PAESLSRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVK 300 PAE++S+MA W+GHFDR + LAFVK LGIYP+GS+V+L+SG + +VIRQN DLT+PV+K Sbjct: 291 PAEAISQMARWEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLK 350 Query: 301 AFVTTPSIEVVESEIIDLSAPGQTDAIAERSGQDWLK 337 E ++DL A +TD I R ++W + Sbjct: 351 VLHLQGPHETTIGGVLDLMAWPRTDTIIGRGDEEWTR 387 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 302 bits (773), Expect = 4e-83, Method: Compositional matrix adjust. Identities = 144/278 (51%), Positives = 194/278 (69%) Query: 42 ELDDALRLYENAKQTTQQMFKQARLGKTLDVENCKSLVEEIATSVARDSNALLSIIRLKR 101 E + A R+ ++K T MF +AR+GK +DV++ LV EI SV+R+++AL+S+ RLK Sbjct: 98 ERERAQRIISSSKSTVLHMFSEARMGKAIDVKDAAELVTEITASVSRNADALISLARLKT 157 Query: 102 VDEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSE 161 D+YTYLHS+AVC +M++LA LGM K+AG+AG LHDVGK +PL+ILNKP +L+E Sbjct: 158 TDDYTYLHSVAVCAMMISLANQLGMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTE 217 Query: 162 QEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAA 221 +E+ ++ H G+ L+ G+ + LDV LHHHEKIDGSGYP + + IS+ ++M A Sbjct: 218 EEFASVRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGA 277 Query: 222 VCDVYDAVTSERPYKSGWDPAESLSRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSG 281 VCDVYDA+TS RPYK+GWDPA S+ MA+ GHFD AFVKA+GIYP GS V LQSG Sbjct: 278 VCDVYDAITSNRPYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSG 337 Query: 282 RTALVIRQNHVDLTKPVVKAFVTTPSIEVVESEIIDLS 319 R +V+ Q L P VK F +T + +ESEIIDL+ Sbjct: 338 RLGVVVDQRPDQLLTPRVKVFYSTANRMPLESEIIDLA 375 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 299 bits (766), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 138/288 (47%), Positives = 199/288 (69%) Query: 31 PEAAGSSSSRSELDDALRLYENAKQTTQQMFKQARLGKTLDVENCKSLVEEIATSVARDS 90 PEA + ++E + A R+ ++++T MF QAR+GKT+DV+ LV EI SV+R++ Sbjct: 90 PEAGKRTDIQAERERAARIISSSEKTVLTMFSQARMGKTIDVKEASDLVREINASVSRNA 149 Query: 91 NALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYLHDVGKAFIPL 150 +A++S++RLK D YTY+HS+AVC +M++LA LG+ ++AG+AG LHD+GK +P Sbjct: 150 DAMISLVRLKTADNYTYMHSVAVCAMMISLADQLGLSAQERQQAGMAGLLHDIGKMTVPP 209 Query: 151 EILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQG 210 ILNKP +L+E+E+ +Q HP G+ L+ G+ D LDV LHHHEK+DG+GYP + G Sbjct: 210 SILNKPSKLTEEEFVSVQSHPLAGHRLLQELPGIGDAPLDVSLHHHEKMDGTGYPYKLSG 269 Query: 211 NQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRMASWQGHFDRPIFLAFVKALGIY 270 IS+ +RM A+CDVYDA+TS+RPYKSGWDP+ S+ MA +GHFD + AFVKA+GIY Sbjct: 270 RDISMTARMGAICDVYDAITSDRPYKSGWDPSRSIQHMAQSKGHFDFDVLQAFVKAVGIY 329 Query: 271 PIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPSIEVVESEIIDL 318 P+GS V +Q+G A+V+ Q L P+V+ F +T V +IDL Sbjct: 330 PVGSCVLMQTGHLAMVLEQRPDSLLAPLVRIFYSTNKKIAVTPTLIDL 377 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,236,898 Number of extensions: 50287 Number of successful extensions: 195 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 10 Length of query: 431 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 343 Effective length of database: 1,203,190 Effective search space: 412694170 Effective search space used: 412694170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)