BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0027.p (385 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 788 0.0 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 141 7e-35 gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [... 106 3e-24 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 76 5e-15 gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 65 1e-11 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 788 bits (2034), Expect = 0.0, Method: Compositional matrix adjust. Identities = 385/385 (100%), Positives = 385/385 (100%) Query: 1 MSQAPSTTAAATRTDAVKFAYWVPNVSGGLVVSTIPQRTDWSLEYNQRLAVTAEKAGFEY 60 MSQAPSTTAAATRTDAVKFAYWVPNVSGGLVVSTIPQRTDWSLEYNQRLAVTAEKAGFEY Sbjct: 1 MSQAPSTTAAATRTDAVKFAYWVPNVSGGLVVSTIPQRTDWSLEYNQRLAVTAEKAGFEY 60 Query: 61 ALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHIS 120 ALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHIS Sbjct: 61 ALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHIS 120 Query: 121 NGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRALKGIWTQDDFTFKGDFYRFN 180 NGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRALKGIWTQDDFTFKGDFYRFN Sbjct: 121 NGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRALKGIWTQDDFTFKGDFYRFN 180 Query: 181 NYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQN 240 NYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQN Sbjct: 181 NYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQN 240 Query: 241 GHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHVEAVHAFGDAVKQAGKASPEGEGNWAK 300 GHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHVEAVHAFGDAVKQAGKASPEGEGNWAK Sbjct: 241 GHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHVEAVHAFGDAVKQAGKASPEGEGNWAK 300 Query: 301 STFEDLVQYNDGFKTNLIGTPQQIAERIVALKAIGVDLVLTGFLHFIEEVEYFGQKVLPL 360 STFEDLVQYNDGFKTNLIGTPQQIAERIVALKAIGVDLVLTGFLHFIEEVEYFGQKVLPL Sbjct: 301 STFEDLVQYNDGFKTNLIGTPQQIAERIVALKAIGVDLVLTGFLHFIEEVEYFGQKVLPL 360 Query: 361 VRELEARQAATAASPAAAPVLEAVA 385 VRELEARQAATAASPAAAPVLEAVA Sbjct: 361 VRELEARQAATAASPAAAPVLEAVA 385 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 141 bits (356), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 106/331 (32%), Positives = 169/331 (51%), Gaps = 20/331 (6%) Query: 42 SLEYNQRLAVTAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILP 101 +LEY +++AVT+E G++ L GA V + +L+ T ++K+L A+ Sbjct: 28 TLEYMRQIAVTSETLGYDGLLIPT------GASCLDPWV-VAASLVPVTQRIKLLVALRT 80 Query: 102 GPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRAL 161 P A+Q AT+D NGR+ +NVV G E +A G +L+HDERY ++E++ Sbjct: 81 SLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGV-FLKHDERYEAADEYLTIW 139 Query: 162 KGIWTQDDFTFKGDFYRFNNYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGN 221 + + + ++G +R N L VQ+P+P ++ GGSS AA D AA D Y T G Sbjct: 140 RKLLQGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGE 199 Query: 222 TLEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDII------DHAHVE 275 ++ +IDD++ +AA+ G VR GV I R+T EA + +I D A + Sbjct: 200 PPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAEAWTAAERLISKLTDEDIARAQ 259 Query: 276 AVHAFGDAVKQAGKASPEG----EGNWAKSTFEDLVQYNDGFKTNLIGTPQQIAERIVAL 331 +A D+V Q A+ G + A + + + G T+L+G +AER+ Sbjct: 260 QNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGGAGTSLVGDATTVAERLQEY 319 Query: 332 KAIGVD-LVLTGFLHFIEEVEYFGQKVLPLV 361 +GVD VL+G+ H +EE F + V PL+ Sbjct: 320 ADLGVDTFVLSGYPH-LEEAIRFAELVFPLL 349 >gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 106 bits (265), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 101/343 (29%), Positives = 163/343 (47%), Gaps = 40/343 (11%) Query: 42 SLEYNQRLAVTAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILP 101 S +Y +++AV A+ G++ L G E ++ + +L+ AT +LK L A+ P Sbjct: 26 SHDYMKQVAVAADTLGYDGVL----LPTGRSCE---DAWVAAASLIDATRRLKFLVAVRP 78 Query: 102 GPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRAL 161 G P + A+ AT D +S GR+ INVV+G E ++ G + H ERY S+EF++ Sbjct: 79 GLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDG-VFGSHAERYEISDEFLKIW 137 Query: 162 KGIWTQDDFTFKGDFYRFNNYTLSPKPVQ-------KPHPEIFQGGSSRAARDNAASVSD 214 + + T+ T G+ F LS K + +P+P ++ GGSS A D AA D Sbjct: 138 RDVLTK---TRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGGSSDEAIDLAAEQVD 194 Query: 215 WYFTNGNTLEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHV 274 Y T G + +I ++ +AA+ G +R G+ VI R+T E A ++I Sbjct: 195 VYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEAAWRAADELISRLDD 254 Query: 275 EAVHAFGDAVKQAGKASPEGEGNWAKSTF----EDLVQYN-----------DGFKTNLIG 319 E + A QA A + EG + D ++ + G T L+G Sbjct: 255 ETI-----AKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVRGGAGTALVG 309 Query: 320 TPQQIAERIVALKAIGVD-LVLTGFLHFIEEVEYFGQKVLPLV 361 +AER+ A+G+D +L+G+ H +EE F + V PL+ Sbjct: 310 DGPTVAERLQEYAALGIDTFILSGYPH-LEESYRFAELVFPLL 351 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 76.3 bits (186), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 70/307 (22%), Positives = 136/307 (44%), Gaps = 51/307 (16%) Query: 106 PAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIG-EPWLEHDERYRRSNEFIRALKGI 164 P + A++++T+DH+S GR+ N+V+G+ +A+G LEHD+RY R++EF+ + + Sbjct: 113 PFLFARRMSTLDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKL 172 Query: 165 WT---QDDFTFK-------------------GDFYRFNNYTLSPKPVQKPHPEIFQGGSS 202 W +D + GD+Y LS +P ++ P ++Q GSS Sbjct: 173 WEGSWEDGAVLRDRARRVYADPAKVHKIAHHGDYYHVEGIHLS-EPSRQRTPVLYQAGSS 231 Query: 203 RAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQNGHK---VRIGVNAFVIARDTEE 259 + A ++ F + + + + + I+ A + G ++I + V+ TE+ Sbjct: 232 GRGQAFGARHAECVFISSQSKDATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQ 291 Query: 260 EAQAVLQDIIDHAHVEA--VH--------------------AFGDAVKQAGKASPEGEGN 297 +AQ ++ +A EA H A +A++ K + E N Sbjct: 292 QAQERYREYARYASPEAGLAHYSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELN 351 Query: 298 WAKSTFEDLVQYNDGFKTNLIGTPQQIAERIVALKA-IGVDLVLTGFLHFIEEVEYFGQK 356 W K ++ G +++G P ++A+ + + A G+D E E F + Sbjct: 352 WTKRKLLQQLELG-GRYASIVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVEL 410 Query: 357 VLPLVRE 363 V+P+++E Sbjct: 411 VIPVLQE 417 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 65.1 bits (157), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 72/270 (26%), Positives = 117/270 (43%), Gaps = 43/270 (15%) Query: 43 LEYNQRLAVTAEKAGFE--YALSQIRFTAGYGAEYQH------------ESVSFSQALLH 88 L+Y +A T E+ F+ + ++ + YG + H + + AL Sbjct: 32 LDYYLHIARTLERGKFDLLFFADRLAVSTRYGDSHLHGLYIGDQDATRLDPLPILGALAA 91 Query: 89 ATTKLKVLAAILPGPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIG-EPWLEH 147 AT+ + + A P +A++ AT+DH+S GR A NVV+ +GE G + L H Sbjct: 92 ATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGRAAWNVVTSVNQGEADNFGLKETLPH 151 Query: 148 DERYRRSNEFIRALKGIWTQ---DDFTFKGDFYRFNNYTLSPK----------PVQKP-- 192 D RY R++EF+ +W D + D Y + + P+ P Sbjct: 152 DVRYDRADEFLEVTHKLWHSWQPDALRLQQDRYADADKVAAIDHQGAHFSVRGPLNIPAS 211 Query: 193 ---HPEIFQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQNGHK---VRI 246 HP I Q GSS +D AA ++ F L L+A D++ +A Q G + +RI Sbjct: 212 PQGHPVIIQAGSSDRGQDFAARWAEVVFNIQPDLPRLQAFYRDLKNRAQQFGRRPQDIRI 271 Query: 247 --GVNAFV-----IARDTEEEAQAVLQDII 269 V FV AR+ +E+A A+ ++ Sbjct: 272 LSAVMPFVGQSRQHARELQEQANALADPLV 301 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,143,586 Number of extensions: 47241 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 138 Number of HSP's successfully gapped: 5 Length of query: 385 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 298 Effective length of database: 1,207,925 Effective search space: 359961650 Effective search space used: 359961650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)