BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0022.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurizatio... 847 0.0 gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbasp... 385 e-108 gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbasp... 370 e-103 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 65 8e-12 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 62 7e-11 >gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurization flavin reductase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 847 bits (2188), Expect = 0.0, Method: Compositional matrix adjust. Identities = 419/419 (100%), Positives = 419/419 (100%) Query: 1 MSAVLAPSLHSTSSAAAPVPGLPRASQPAHVIRDDAEAIAVAHALAPRLAQGASERDQLR 60 MSAVLAPSLHSTSSAAAPVPGLPRASQPAHVIRDDAEAIAVAHALAPRLAQGASERDQLR Sbjct: 1 MSAVLAPSLHSTSSAAAPVPGLPRASQPAHVIRDDAEAIAVAHALAPRLAQGASERDQLR 60 Query: 61 RWPVEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVA 120 RWPVEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVA Sbjct: 61 RWPVEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVA 120 Query: 121 AVRTVSDPEQQRRFFADFLRGVRWGNAFSESGSKRAADFETRFVDAGDHVIVTGKKFYST 180 AVRTVSDPEQQRRFFADFLRGVRWGNAFSESGSKRAADFETRFVDAGDHVIVTGKKFYST Sbjct: 121 AVRTVSDPEQQRRFFADFLRGVRWGNAFSESGSKRAADFETRFVDAGDHVIVTGKKFYST 180 Query: 181 GALLAHYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKT 240 GALLAHYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKT Sbjct: 181 GALLAHYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKT 240 Query: 241 HLIPAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWED 300 HLIPAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWED Sbjct: 241 HLIPAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWED 300 Query: 301 PYSIQAIGDLQIRLHASEALLERAGRAIDAAVAAPDAQSVAHAQILTAEAKVLSTEIAIH 360 PYSIQAIGDLQIRLHASEALLERAGRAIDAAVAAPDAQSVAHAQILTAEAKVLSTEIAIH Sbjct: 301 PYSIQAIGDLQIRLHASEALLERAGRAIDAAVAAPDAQSVAHAQILTAEAKVLSTEIAIH 360 Query: 361 ATNKLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHAWS 419 ATNKLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHAWS Sbjct: 361 ATNKLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHAWS 419 >gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 421 Score = 385 bits (990), Expect = e-108, Method: Compositional matrix adjust. Identities = 211/388 (54%), Positives = 269/388 (69%), Gaps = 2/388 (0%) Query: 32 IRDDAEAIAVAHALAPRLAQGASERDQLRRWPVEEIEAYSQSGLWAINVPRAFGGAEVSY 91 IRDDAEAIA A LA ++ A+ RD+ R+ P +E+E Y +SGLW I VP+ +GGA VSY Sbjct: 32 IRDDAEAIAAARKLAEDFSREAALRDRERKLPWDELERYVRSGLWGITVPKEYGGAGVSY 91 Query: 92 ATLAEVIAIISAADPSLGQITQNHLGFVAAVRTVSDPEQQRRFFADFLRGVRWGNAFSES 151 TLAEVIA ISAAD S+GQI QNH V +R Q+R FF L+G R+GNA +E Sbjct: 92 GTLAEVIATISAADGSIGQIPQNHFYAVELIRVNGSESQKRFFFERVLQGDRFGNALAEL 151 Query: 152 GSKRAADFETRFVDA--GDHVIVTGKKFYSTGALLAHYVPIVANDEENRTWYVVAERNAP 209 G+K + D +TR G + G+KFY+TGAL A +VP D++ + R+AP Sbjct: 152 GTKTSHDRKTRLSRNAHGPGYRIDGRKFYATGALYADWVPTSVIDDDGVQQLAIVPRHAP 211 Query: 210 GLTVIDDWSAFGQRTTLSGTVIIDQVKVPKTHLIPAYKGYLEPSADGAIFQIIQAAVDVG 269 GLT+IDDWS FGQRTT SG+ + + V VP +I + P+ G + QI AA+D+G Sbjct: 212 GLTIIDDWSGFGQRTTGSGSAVFENVHVPADAVIGFATAFSRPTQIGPMAQIQHAAIDLG 271 Query: 270 IARAAIADTQDFVRTKSRPWIDSQRDHAWEDPYSIQAIGDLQIRLHASEALLERAGRAID 329 IARAA AD FVRT+SR W+DS + A +DP +I+ +GDL I+LHA+EALL RAGR +D Sbjct: 272 IARAAYADLLTFVRTRSRAWVDSGVERAVDDPLTIREVGDLTIQLHAAEALLRRAGRKVD 331 Query: 330 AAVAAPDAQSVAHAQILTAEAKVLSTEIAIHATNKLFELAGTRSTLGQYNLDRHWRNART 389 AA AAPD SVA A I AEA+VL+TE+++ A +KLFELAG++STL ++NLDRHWRNART Sbjct: 332 AAAAAPDEDSVAAASIAVAEARVLTTEVSLAAGSKLFELAGSQSTLAEHNLDRHWRNART 391 Query: 390 HTLHDPVRWKYAIVGNYYLNGVKPPFHA 417 HTLHDPVRWKY +GNY LNG KPP H Sbjct: 392 HTLHDPVRWKYHAIGNYVLNGKKPPRHG 419 >gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 370 bits (949), Expect = e-103, Method: Compositional matrix adjust. Identities = 209/410 (50%), Positives = 272/410 (66%), Gaps = 4/410 (0%) Query: 14 SAAAPVPGLPRASQPAHVIRDDAEAIAVAHALAPRLAQGASERDQLRRWPVEEIEAYSQS 73 SA P P +PA I ++ A+ VA LA A A++RD+ R P E+IE +SQS Sbjct: 2 SAHIPEPIRRAGQRPAQRISSESHALQVAAELAAEFATSAAQRDRERILPFEQIERFSQS 61 Query: 74 GLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVAAVRTVSDPEQQRR 133 GLWAI VPR+ GG S+ T+AEVI +ISAADPS+GQI QNH G + + ++ EQQR Sbjct: 62 GLWAITVPRSHGGIGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIARLASQEQQRL 121 Query: 134 FFADFLRGVRWGNAFSESGSKRAADFETRFV---DAGD-HVIVTGKKFYSTGALLAHYVP 189 FFA+ L G R GNA E K D TR AGD ++ G +FYSTGAL AH++P Sbjct: 122 FFAEVLDGARLGNAGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWIP 181 Query: 190 IVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKTHLIPAYKGY 249 A D++ + V R+APG+ VIDDWS+FGQRTT SGTV+ D V+VP T ++ Sbjct: 182 SRAIDDDGQPLVVYTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLALKDAL 241 Query: 250 LEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWEDPYSIQAIGD 309 +P+ G + Q+IQAA+D GIA AA+ DT FV T+SRP++DS + A +DPY ++ IG+ Sbjct: 242 QQPNNIGPVSQLIQAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIGN 301 Query: 310 LQIRLHASEALLERAGRAIDAAVAAPDAQSVAHAQILTAEAKVLSTEIAIHATNKLFELA 369 L +RLHA++ALLE A +D+ A + +A A AEAKVLSTE+A+ A+ KLFEL+ Sbjct: 302 LSLRLHAADALLEDAAALLDSLPAQLSFEQMAQASAAVAEAKVLSTELALLASEKLFELS 361 Query: 370 GTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHAWS 419 G+ +TL +NLDRHWRNAR HTLHDPVRWKY VGNY LNG PP H+WS Sbjct: 362 GSAATLASHNLDRHWRNARVHTLHDPVRWKYFAVGNYALNGTPPPRHSWS 411 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 65.5 bits (158), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 93/384 (24%), Positives = 162/384 (42%), Gaps = 44/384 (11%) Query: 28 PAH-VIRDDAEAIAVAHALAPRLAQGASERDQLRRWPVEEIEAYSQSGLWAINVPRAFGG 86 P H +IRD A+ H RL A++ D+ +P E ++ ++ G + VP +GG Sbjct: 5 PEHEMIRD-----AMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGG 59 Query: 87 AEVSYATLAEVIAIISAADPSLGQI--TQNHLGFVAAVRTVSDPEQQRRFFADFLRGVRW 144 A + Y +L + I+A D + I QN L P Q+ ++ RG Sbjct: 60 AGMDYMSLVLALEEIAAGDGATSTIVSVQNSLA-CGITEKYGSPTQKEQWLKPLARGEML 118 Query: 145 GN-AFSE--SGSKRAADFETRFVDAGDHVIVTG-KKFYSTGALLAHYVPIVAND----EE 196 G +E +GS AA TR GD I+ G K+F ++G + D ++ Sbjct: 119 GCFCLTEPHTGSDAAA-IRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKK 177 Query: 197 NRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKTHLIPAY-KGYLEPSAD 255 + ++V + PG V GQ + + +++D +VP T L+ +GY ++ Sbjct: 178 GISCFLVP-CDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSN 236 Query: 256 GAIFQIIQAAVDVGIARAAIADTQDFVRTKSR---PWIDSQRDHAWEDPYSIQAIGDLQI 312 +I AA VG+ARAA + R + P I+ Q + + D+ Sbjct: 237 LEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFR--------LADMNT 288 Query: 313 RLHASEALLERAGRAIDAAVAAPDAQSVAHAQILTAEA--KVLSTEIAIHATNKLFELAG 370 L A+ ++ RA + D P + + A++ +EA K+ S I IH G Sbjct: 289 LLDAARLMVWRAAQLKDQ--GRPCLKEASMAKMFASEAAEKIASDAIQIH---------G 337 Query: 371 TRSTLGQYNLDRHWRNARTHTLHD 394 + ++R +R+ R +++ Sbjct: 338 GVGYTSDFPVERIYRDVRICQIYE 361 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 62.4 bits (150), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 84/359 (23%), Positives = 152/359 (42%), Gaps = 31/359 (8%) Query: 46 APRLAQGASERDQLRRWPVEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAAD 105 A +A A++ D+ +P++ I + G + P A GG +S V ++ A Sbjct: 21 AGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGLSRLDATVVFEELARAC 80 Query: 106 PSLGQITQNHLGFVAAVRTVSDPEQQRRFFADFLRGVRWGN-AFSESGS-KRAADFETRF 163 S H + + P + ++ G + G+ +E GS AA +T Sbjct: 81 TSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGRKIGSYCLTEPGSGSDAASLKTTA 140 Query: 164 VDAGDHVIVTGKKFYSTGALLAHYVPIVA---NDEENRTWYVVAERNAPGLTVIDDWSAF 220 H ++ G K + +GA + ++A + +V NAPG++ S Sbjct: 141 RLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEGARGVSAIVVPGNAPGVSYGKKESKM 200 Query: 221 GQRTTLSGTVIIDQVKVPKTHLIPAY-KGYL--EPSADGAIFQIIQAAVDVGIARAAIAD 277 G + + T+ D VKVP HL+ +G++ DG I A VG A+AA+ Sbjct: 201 GWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRINI--ATCSVGAAQAALDA 258 Query: 278 TQDFVRTK---SRPWIDSQRDHAWEDPYSIQ-AIGDLQIRLHASEALLERAGRAIDAAVA 333 +++ + RP D Q ++Q + D+Q L A+ ++ A +DA Sbjct: 259 AHAYMKERKQFGRPIADFQ---------ALQFKLADMQTELVAARQMVRLAATKLDAG-- 307 Query: 334 APDAQSVAHAQILTAEAKVLSTEIAIHATNKLFELAGTRSTLGQYNLDRHWRNARTHTL 392 +P+A + A AK L+T++ N+ +L G + +Y L+R++R+ R H + Sbjct: 308 SPEATTYC------AMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQI 360 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,308,953 Number of extensions: 53719 Number of successful extensions: 212 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 209 Number of HSP's successfully gapped: 6 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)