BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0007.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 827 0.0 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 516 e-147 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 473 e-134 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 306 1e-84 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 300 1e-82 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 827 bits (2136), Expect = 0.0, Method: Compositional matrix adjust. Identities = 400/400 (100%), Positives = 400/400 (100%) Query: 1 VEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGLDVAQEE 60 VEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGLDVAQEE Sbjct: 7 VEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGLDVAQEE 66 Query: 61 EAADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALRETAKKATKAMFSEARMGKVLDME 120 EAADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALRETAKKATKAMFSEARMGKVLDME Sbjct: 67 EAADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALRETAKKATKAMFSEARMGKVLDME 126 Query: 121 RCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDETACRE 180 RCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDETACRE Sbjct: 127 RCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDETACRE 186 Query: 181 AGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDYARDVCL 240 AGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDYARDVCL Sbjct: 187 AGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDYARDVCL 246 Query: 241 HHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAISAMASWTG 300 HHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAISAMASWTG Sbjct: 247 HHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAISAMASWTG 306 Query: 301 HFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYDDSMTVTDT 360 HFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYDDSMTVTDT Sbjct: 307 HFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYDDSMTVTDT 366 Query: 361 EILDLLANPEADTIVARGGEEWARLSHLDAMQLLSTGVPT 400 EILDLLANPEADTIVARGGEEWARLSHLDAMQLLSTGVPT Sbjct: 367 EILDLLANPEADTIVARGGEEWARLSHLDAMQLLSTGVPT 406 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 516 bits (1329), Expect = e-147, Method: Compositional matrix adjust. Identities = 253/390 (64%), Positives = 297/390 (76%), Gaps = 3/390 (0%) Query: 1 VEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGLDVAQEE 60 V DLVLGMYFCGFEA+WL HPFWK +FLL +S+L+QA ASGIAYCWID RG DVA Sbjct: 7 VGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGRDVAGAA 66 Query: 61 EAADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALRETAKKATKAMFSEARMGKVLDME 120 A AA + HA A DA A+ALRE A++ T+ MF+ ARMGK++++ Sbjct: 67 PAKAEAAAAPASPDQSAATHAGA---DADRASAVALREHAREVTRQMFNAARMGKMVELP 123 Query: 121 RCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDETACRE 180 +CV++VEEIA SV++ PNG LSVLRLKL DEYTYLHSV+VCAL++ LGRTLGL E ACRE Sbjct: 124 QCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGLGRTLGLSEAACRE 183 Query: 181 AGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDYARDVCL 240 AGL G+LHDVGKAFI E+LNKPGKL+ EEF+ IK+HPELGY+YL Q P++P YA DVC Sbjct: 184 AGLSGYLHDVGKAFIDDEILNKPGKLTAEEFALIKRHPELGYQYLIQTPDMPAYASDVCR 243 Query: 241 HHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAISAMASWTG 300 HHHE++DG GYP+ L A +ITLY+RM A+CDVYDALTS+RPYK WDPAEAIS MA W G Sbjct: 244 HHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEAISQMARWEG 303 Query: 301 HFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYDDSMTVTDT 360 HFDR + LAFVKTLGIYPVGS+V+LESG SG VIRQN DLTRPVLKV H T Sbjct: 304 HFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHLQGPHETTIG 363 Query: 361 EILDLLANPEADTIVARGGEEWARLSHLDA 390 +LDL+A P DTI+ RG EEW RL DA Sbjct: 364 GVLDLMAWPRTDTIIGRGDEEWTRLRQQDA 393 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 473 bits (1217), Expect = e-134, Method: Compositional matrix adjust. Identities = 224/388 (57%), Positives = 285/388 (73%), Gaps = 8/388 (2%) Query: 1 VEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGLDVAQEE 60 V L +GMYF GF+ AW+D PFW+ FL++ SE+ +A A+G YCWIDT++G+D+A E Sbjct: 9 VGQLSVGMYFSGFDGAWIDTPFWRTSFLVRKASEIARARAAGYTYCWIDTDQGIDLAGAE 68 Query: 61 EAADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALRETAKKATKAMFSEARMGKVLDME 120 A L ETE EPE A + + L DA+ L E AK+ T+ MF +AR+GK LD+E Sbjct: 69 -----ADRLPETE---EPEAAGSSSSRSELDDALRLYENAKQTTQQMFKQARLGKTLDVE 120 Query: 121 RCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDETACRE 180 C S+VEEIA SV + N LS++RLK DEYTYLHS+AVC LMVAL RTLG+D+ AC+E Sbjct: 121 NCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKE 180 Query: 181 AGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDYARDVCL 240 AGL G+LHDVGKAFIP E+LNKP +LS++E+ I++HP LGY YL VPDY DVCL Sbjct: 181 AGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCL 240 Query: 241 HHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAISAMASWTG 300 HHHEKIDG+GYP + I++YSRMAA+CDVYDA+TS+RPYK WDPAE++S MASW G Sbjct: 241 HHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRMASWQG 300 Query: 301 HFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYDDSMTVTDT 360 HFDR +FLAFVK LGIYP+GSIVKL+SGR+ VIRQN DLT+PV+K F S+ V ++ Sbjct: 301 HFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPSIEVVES 360 Query: 361 EILDLLANPEADTIVARGGEEWARLSHL 388 EI+DL A + D I R G++W + + L Sbjct: 361 EIIDLSAPGQTDAIAERSGQDWLKSNGL 388 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 306 bits (785), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 163/390 (41%), Positives = 224/390 (57%), Gaps = 11/390 (2%) Query: 2 EDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGLDVAQEEE 61 + L +GMY F AW P W+ FLL+ D +L AS I ID +G DV Q +E Sbjct: 8 KQLRIGMYIQSFSGAWRRPPAWRKPFLLEKDKDLLALQASPIREIVIDIAKGADV-QTDE 66 Query: 62 AADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALRETA-------KKATKAMFSEARMG 114 A + E E A A P D A RE A +K MFS+ARMG Sbjct: 67 AEQGNGVESSLGKNAEIEVAQALPEAGKRTDIQAERERAARIISSSEKTVLTMFSQARMG 126 Query: 115 KVLDMERCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTLGLD 174 K +D++ +V EI SV ++ + +S++RLK AD YTY+HSVAVCA+M++L LGL Sbjct: 127 KTIDVKEASDLVREINASVSRNADAMISLVRLKTADNYTYMHSVAVCAMMISLADQLGLS 186 Query: 175 ETACREAGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDY 234 ++AG+ G LHD+GK +P +LNKP KL++EEF ++ HP G++ L + P + D Sbjct: 187 AQERQQAGMAGLLHDIGKMTVPPSILNKPSKLTEEEFVSVQSHPLAGHRLLQELPGIGDA 246 Query: 235 ARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAISA 294 DV LHHHEK+DG GYP L I++ +RM AICDVYDA+TSDRPYK WDP+ +I Sbjct: 247 PLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAICDVYDAITSDRPYKSGWDPSRSIQH 306 Query: 295 MASWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYDDS 354 MA GHFD + AFVK +GIYPVGS V +++G V+ Q L P++++F+ + Sbjct: 307 MAQSKGHFDFDVLQAFVKAVGIYPVGSCVLMQTGHLAMVLEQRPDSLLAPLVRIFYSTNK 366 Query: 355 MTVTDTEILDLLANPEADTIV-ARGGEEWA 383 ++DL A+ IV A E+W Sbjct: 367 KIAVTPTLIDL--RRSANKIVGAVDPEKWG 394 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 300 bits (768), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 155/368 (42%), Positives = 220/368 (59%), Gaps = 3/368 (0%) Query: 1 VEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGLDVAQEE 60 ++ L LGMY W+ HPF + F ++ +L+ I I+T +G DV +EE Sbjct: 7 LDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGRDVTREE 66 Query: 61 -EAADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALR--ETAKKATKAMFSEARMGKVL 117 E D+ P+ A D + A R ++K MFSEARMGK + Sbjct: 67 LEEDDADQDAPAAPPADAANRNPASRTDTAIERERAQRIISSSKSTVLHMFSEARMGKAI 126 Query: 118 DMERCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDETA 177 D++ +V EI SV ++ + +S+ RLK D+YTYLHSVAVCA+M++L LG+ Sbjct: 127 DVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLANQLGMTPQQ 186 Query: 178 CREAGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDYARD 237 ++AG+ G LHDVGK +P ++LNKP KL++EEF+ ++ H G+ L Q + + A D Sbjct: 187 TKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQIEGIGEVALD 246 Query: 238 VCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAISAMAS 297 V LHHHEKIDG+GYP L+A +I++ ++M A+CDVYDA+TS+RPYK WDPA +I MA+ Sbjct: 247 VSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARSIRHMAA 306 Query: 298 WTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYDDSMTV 357 GHFD AFVK +GIYP GS V L+SGR G V+ Q L P +KVF+ + Sbjct: 307 SAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKVFYSTANRMP 366 Query: 358 TDTEILDL 365 ++EI+DL Sbjct: 367 LESEIIDL 374 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,221,131 Number of extensions: 49095 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 10 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)