BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0088.p (249 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 487 e-139 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 236 9e-64 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 130 8e-32 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 125 2e-30 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 125 4e-30 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 487 bits (1253), Expect = e-139, Method: Compositional matrix adjust. Identities = 248/249 (99%), Positives = 248/249 (99%) Query: 1 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPG 60 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPG Sbjct: 3 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPG 62 Query: 61 DAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLF 120 DAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLF Sbjct: 63 DAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLF 122 Query: 121 SVQKALPLMSQGGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIRVNV 180 SVQKALPLMSQGGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIRVNV Sbjct: 123 SVQKALPLMSQGGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIRVNV 182 Query: 181 VAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEADEIGRAVLFLASAESSYIAGVEL 240 VAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGE DEIGRAVLFLASAESSYIAGVEL Sbjct: 183 VAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSYIAGVEL 242 Query: 241 FVDGGSVAV 249 FVDGGSVAV Sbjct: 243 FVDGGSVAV 251 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 236 bits (603), Expect = 9e-64, Method: Compositional matrix adjust. Identities = 132/251 (52%), Positives = 174/251 (69%), Gaps = 4/251 (1%) Query: 1 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPG 60 M K+ K+ +VTGG+ GIGLATA+ AA+GA+V+++GRR +L+ AV IG A G+ Sbjct: 1 MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA 60 Query: 61 DAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLF 120 DA+ L +LD +YA++ GRLD+LFANAG + PLG I EE FD +F NVRG+LF Sbjct: 61 DASVLSELDTVYAQIAKSAGRLDILFANAGGG--DMMPLGAITEEHFDRIFGTNVRGVLF 118 Query: 121 SVQKALPLMSQGGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIRVNV 180 +VQKALPL+ G +++L S V G S+Y+ASKAAVR+FARSW DLK+R IRVNV Sbjct: 119 TVQKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNV 178 Query: 181 VAPGGTETRLMRDYL--EARPGMEDVLKQVVPLGRLGEADEIGRAVLFLASAESSYIAGV 238 V+PG T + + + +AR G+ D L VPLGRLGE EIG+AV FLAS +S++ G+ Sbjct: 179 VSPGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNGI 238 Query: 239 ELFVDGGSVAV 249 ELFVDGG V Sbjct: 239 ELFVDGGMAQV 249 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 130 bits (328), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 93/248 (37%), Positives = 137/248 (55%), Gaps = 12/248 (4%) Query: 5 FEDKVVVVTGGS--EGIGLATAKLFAAEGARVYVSG-RRADKLEQAVREIGNGAVGVPGD 61 +DKV ++TGG+ G+G +TA+L AA+GAR+ + RA+ A R +G +G+ D Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAAR-LGAQHLGLVAD 61 Query: 62 AAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLFS 121 + A V GR+D+L NAG + AK L + G +D+D + ++++RG L+ Sbjct: 62 VTDKAACEAAAAAVLKQFGRIDILVNNAGV-TQPAKLLEITG-KDYDRILDVSLRGTLYM 119 Query: 122 VQKALPLM--SQGGAIVLNGSVAGVK--GFPGQSLYNASKAAVRSFARSWTADLKERGIR 177 Q LP M Q G+IV SV+ + G G Y+A+KA V AR+ + +GIR Sbjct: 120 SQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIR 179 Query: 178 VNVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEADEIGRAVLFLASAESSYIAG 237 VN + PG T + +D L A E + Q +PLGRLG D++G +FLAS S Y G Sbjct: 180 VNCITPGLISTDINKDILSAEKKAE--IAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTG 237 Query: 238 VELFVDGG 245 V L V+GG Sbjct: 238 VTLDVNGG 245 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 125 bits (315), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 87/253 (34%), Positives = 138/253 (54%), Gaps = 16/253 (6%) Query: 4 RFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRAD-------KLEQAVREIGNGAV 56 R +DKV +VTGG G G AK +A EGA + V AD ++ + ++ G AV Sbjct: 2 RLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVV----ADIGEAGGLRVVEEIKAAGGRAV 57 Query: 57 GVPGDAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVR 116 D +K D+D+L A G+LD++ NAGT + +P+ + E++FD ++ +NV+ Sbjct: 58 FAKADVSKRADMDQLLATALEHFGKLDIVVNNAGT-THRNRPMLEVEEDEFDRVYAVNVK 116 Query: 117 GLLFSVQKALPLMSQ--GGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKER 174 + S + +P Q GGA + S AG++ PG + YN SK AV + ++S A+L Sbjct: 117 SIFLSAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPD 176 Query: 175 GIRVNVVAPGGTETRLMRDYLEARPGMEDVLKQV--VPLGRLGEADEIGRAVLFLASAES 232 IRVN V P + T L+ +++ E+ K V +P+GR ++I A L+L S E+ Sbjct: 177 KIRVNCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEA 236 Query: 233 SYIAGVELFVDGG 245 ++ GV + VDGG Sbjct: 237 EFVTGVCIEVDGG 249 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 125 bits (313), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 88/247 (35%), Positives = 129/247 (52%), Gaps = 10/247 (4%) Query: 5 FEDKVVVVTGGS--EGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPGDA 62 +DKV V+TGG+ G+G ATA+L A +GARV V A IG+ +G+ D Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADV 62 Query: 63 AKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLFSV 122 + A + GR+D+L NAG + K L + G D+D + ++++RG L+ Sbjct: 63 TDKASCETAAAAILQQFGRIDILVNNAGI-TQPVKTLEITG-ADYDRILDVSLRGTLYMS 120 Query: 123 QKALPLM--SQGGAIVLNGSVAGVK--GFPGQSLYNASKAAVRSFARSWTADLKERGIRV 178 Q LP M Q G+I+ SV+ + G G Y+A+KA V AR+ + GIRV Sbjct: 121 QAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGIRV 180 Query: 179 NVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEADEIGRAVLFLASAESSYIAGV 238 N + PG T +++ L E + + +PL RLG AD+IG A +FL S S Y G+ Sbjct: 181 NSITPGLIGTDIIKGKLTEEKKAE--IAETIPLARLGRADDIGGACVFLGSDLSQYCTGI 238 Query: 239 ELFVDGG 245 L V+GG Sbjct: 239 TLDVNGG 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 822,143 Number of extensions: 36491 Number of successful extensions: 305 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 49 Length of query: 249 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 166 Effective length of database: 1,226,865 Effective search space: 203659590 Effective search space used: 203659590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)