BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0086.p (342 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 680 0.0 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 118 9e-28 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 114 1e-26 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 85 9e-18 gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydro... 74 2e-14 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 680 bits (1754), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/342 (99%), Positives = 341/342 (99%) Query: 1 MSPATMKAIQQHAFGGPEVLRYENAAVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPP 60 M+PATMKAIQQHAFGGPEVLRYE+A VPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPP Sbjct: 1 MTPATMKAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPP 60 Query: 61 EWQPTVAFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVA 120 EWQPTVAFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVA Sbjct: 61 EWQPTVAFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVA 120 Query: 121 ASEIALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNG 180 ASEIALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNG Sbjct: 121 ASEIALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNG 180 Query: 181 AAGGVGHFALQLAKWKGARVIAIGSGGHEAFLRDLGADEFIDYTRSAAEDVLSDVDLVID 240 AAGGVGHFALQLAKWKGARVIAIGSGGHEAFLRDLGADEFIDYTRSAAEDVLSDVDLVID Sbjct: 181 AAGGVGHFALQLAKWKGARVIAIGSGGHEAFLRDLGADEFIDYTRSAAEDVLSDVDLVID 240 Query: 241 AVGGPASGRFLRTLKRGGALFPIFPLGFSGAEEAARLGVNVSTTQVRSSGAQLQQLAGLL 300 AVGGPASGRFLRTLKRGGALFPIFPLGFSGAEEAARLGVNVSTTQVRSSGAQLQQLAGLL Sbjct: 241 AVGGPASGRFLRTLKRGGALFPIFPLGFSGAEEAARLGVNVSTTQVRSSGAQLQQLAGLL 300 Query: 301 DSGVVRVAIDSTFALQDAGKAHERAAQGHIRGKIVLSVIDQG 342 DSGVVRVAIDSTFALQDAGKAHERAAQGHIRGKIVLSVIDQG Sbjct: 301 DSGVVRVAIDSTFALQDAGKAHERAAQGHIRGKIVLSVIDQG 342 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 118 bits (295), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 109/355 (30%), Positives = 173/355 (48%), Gaps = 52/355 (14%) Query: 6 MKAIQQHAFGGPEVLRYENAAVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQPT 65 M+AI+ FGGPEVL+ VPEL+ GEVL++VHA G+N PD + R G +PP Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASD- 59 Query: 66 VAFPLILGTDISGVVEAVADDVKD--FAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASE 123 + G +++G E VA D+ D F +GD V ++V+ GG YA++ + ++ Sbjct: 60 -----LPGLEVAG--EIVAGDLGDSGFKLGDLVCALVQ------GGG--YAEFCAAPLAQ 104 Query: 124 IALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAG 183 P+ +S +AAA P + T W + + G ++T++V G + Sbjct: 105 CLPVPKGLSALEAAALPENYFTVWSNVFDRGQ--------------LSGEETLLVQGGSS 150 Query: 184 GVGHFALQLAKWKGARVIAI-GSGGHEAFLRDLGADEFIDYTRSAAEDVLSD------VD 236 G+G A+Q+A+ G RV A GS +LGA+ I+Y E V+ + VD Sbjct: 151 GIGVTAIQIARALGHRVFATAGSAEKCRACEELGAERAINYKTEDFEAVVKELTGGRGVD 210 Query: 237 LVIDAVGGPASGRFLRTLKRGGALFPIFPLGFS------GAEEAARLGVNVSTTQVR--- 287 +++D VGG R ++ L G L I LG + G RL + ST + R Sbjct: 211 VILDMVGGDYLPREIKALADDGRLVFIAQLGGAKGQLDMGQVMRRRLTITGSTLRPRPVA 270 Query: 288 ---SSGAQLQQ-LAGLLDSGVVRVAIDSTFALQDAGKAHERAAQGHIRGKIVLSV 338 + A+L++ + L+++G +R I F L+ A +AH GKI+L + Sbjct: 271 FKAAIAAKLREHVWPLIEAGKIRPVIHHRFPLEQAAQAHAMMEGSTHIGKIMLEL 325 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 114 bits (285), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 105/336 (31%), Positives = 154/336 (45%), Gaps = 31/336 (9%) Query: 6 MKAIQQHAFGGPEVLRYENAAVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQPT 65 M+AI H +G P VL + L PG V V + A G+ P D LR G Q Sbjct: 1 MRAICYHQYGPPSVLHLCDLDEQPLPPGHVRVAMQAAGVAPVDTKLRAG------RLQHL 54 Query: 66 VA--FPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASE 123 VA P + G D +GV+ A+ + V VG+ V M PS +AG A+ V + + Sbjct: 55 VALDLPKVPGRDGAGVITALGEGVHTLQVGEAVCVM---PS-MAGRGTTLAELV-LPQEQ 109 Query: 124 IALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAG 183 + KP +S QAAA +AW +++ P+ K V ++ +G Sbjct: 110 VVRKPDVLSMQQAAALLQPACSAWIALMQTA-------------PIAAGMK-VFIHAGSG 155 Query: 184 GVGHFALQLAKWKGARVIAIGSGGHEAFLRDLGADEFIDYTRSAAEDVLSDVDLVIDAVG 243 VG +QLA GA V A + ++ LGA + Y R V D D+VID VG Sbjct: 156 AVGSLMVQLAAHLGAEVTASCRSSNVEYVAALGASRVLAYDRDELGRV-RDQDVVIDLVG 214 Query: 244 GPASGRFLRTLKRGGALFPIFPLGFSGAEEAARLGVNVSTTQVRSSGAQLQQLAGLLDSG 303 G A + R L+RGG L ++ + E GV+V + + LQQ+A L G Sbjct: 215 GAAHDQSYRMLRRGGQL--VWLVAAPIRERGEEFGVSVRRALIVEDQSILQQVAALAQQG 272 Query: 304 VVRVAIDSTFALQDAGKAHERAAQGHI-RGKIVLSV 338 V R A+ +Q+ +AH+R G + RG++VL + Sbjct: 273 VWRPAVSGVLPMQEIARAHQRIEAGEVTRGRLVLDI 308 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 84.7 bits (208), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 106/337 (31%), Positives = 150/337 (44%), Gaps = 52/337 (15%) Query: 7 KAIQQHAFGGPEVLRYENAAVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQPTV 66 KAI+ GGPEV+ Y + V E GEV +R AVGLN D Y R G L P QP Sbjct: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAG---LYP--QP-- 55 Query: 67 AFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEIAL 126 P LG + +G +EAV V F VGD V R P+G AYA+ + A + Sbjct: 56 -LPAGLGQEAAGTIEAVGAGVSGFKVGDRVAYAGR-PNG------AYAEERVMPADILVK 107 Query: 127 KPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAGGVG 186 P IS AAA + +T Q+++ + +Q T++ + AAGG+G Sbjct: 108 LPDAISFDTAAAMMLQGMTV-QYLLRQTYAVQ-------------PGDTILFHAAAGGIG 153 Query: 187 HFALQLAKWKGARVIAIGSGGHEAFLR-DLGADEFIDY-TRSAAEDVLS-----DVDLVI 239 A Q A+ G +I S +A L GA I+Y T + E V V +V Sbjct: 154 LIACQWARALGVNLIGTVSSDEKAALALQHGAAHVINYKTENFVERVKELTGGRGVQVVY 213 Query: 240 DAVGGPASGRFLRTLKRGGALF-------PIFPLGFSGAEEAARLGVNV---STTQVRSS 289 D++G L L+ G + P+ P G S E A+R + + S S+ Sbjct: 214 DSIGKDTFLGSLDCLRPRGMMVSFGNASGPVAPFGVS--ELASRGSLFLTRPSLGNYTST 271 Query: 290 GAQLQQLAGLLDSGV----VRVAIDSTFALQDAGKAH 322 A+L AG L V + + I+ +AL+D +AH Sbjct: 272 RAELDATAGDLFDMVLGNKISIEINQRYALKDVAQAH 308 >gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 73.6 bits (179), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 101/359 (28%), Positives = 149/359 (41%), Gaps = 57/359 (15%) Query: 6 MKAI----QQHAFGGPEVLRYENAAVPELQPGE--VLVRVHAVGLNPPDWYLREGYKMLP 59 MKAI Q P+ L +P+ PGE +LV V A+ +NP D +R K Sbjct: 1 MKAILTQPQSPLISAPDALI--ETTLPDPTPGEHDLLVEVKAISVNPVDTKIRASAKE-- 56 Query: 60 PEWQPTVAFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSV 119 +LG D+SGVV AV V FA GDEV F +G A ++ V Sbjct: 57 ---------ARVLGWDVSGVVRAVGPAVTLFAPGDEV-----FYAGSITRPGANSELHVV 102 Query: 120 AASEIALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVN 179 + KP + HA AAA P++ +TAW+ + + + Q + +++ Sbjct: 103 DERIVGRKPSSLGHADAAAIPLTAITAWELLFDRVAVAEGGGQ----------GQHLLII 152 Query: 180 GAAGGVGHFALQLAKWKGARVIAIGSGGHEA---FLRDLGADEFIDYTRSAAEDV----L 232 GAAGGVG QLA+ K ++ IG+ ++ +LGA ID+++ + + Sbjct: 153 GAAGGVGSMLTQLAR-KLTQLTVIGTASRPETAHWVGELGAHHVIDHSQPLKPQLEKLGI 211 Query: 233 SDVDLVIDAVGGPAS-GRFLRTLKRGGALFPI--------FPLGFSGAEEAARLGVNVST 283 +V +VI + + L G L I PL L S Sbjct: 212 PEVGIVISLTHTDQHYAQIIDVLAPQGQLALIDDPASLDAMPLKRKSISLHWELMFTRSM 271 Query: 284 TQVRSSGAQ---LQQLAGLLDSGVVRVAIDSTFALQDAG---KAHERAAQGHIRGKIVL 336 Q Q LQ+++ LLD GV+R + DA +AH G +GKIVL Sbjct: 272 FQTPDMIRQHELLQRVSQLLDQGVLRTTTGEHYGRIDAANLRRAHGLLESGKAKGKIVL 330 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,087,039 Number of extensions: 45813 Number of successful extensions: 180 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 5 Length of query: 342 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 256 Effective length of database: 1,212,660 Effective search space: 310440960 Effective search space used: 310440960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)