BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0077.p (405 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312803|ref|YP_003776895.1| two component sensor histidine ... 766 0.0 gi|300312674|ref|YP_003776766.1| two-component system transcript... 177 2e-45 gi|300311807|ref|YP_003775899.1| light-regulated signal transduc... 115 9e-27 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 84 2e-17 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 80 2e-16 >gi|300312803|ref|YP_003776895.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 766 bits (1977), Expect = 0.0, Method: Compositional matrix adjust. Identities = 388/405 (95%), Positives = 393/405 (97%), Gaps = 4/405 (0%) Query: 1 MSGRPILRLSDFIRSRMEPILQAWETFAKTIHPPALTMDSKALRNHASLMLAAIADDLEK 60 MSGRPILRLSDFIRSRMEPIL+AWE FAKTIHPPALTMDSKALRNHASLMLAAIADDLEK Sbjct: 1 MSGRPILRLSDFIRSRMEPILEAWEAFAKTIHPPALTMDSKALRNHASLMLAAIADDLEK 60 Query: 61 TQTEEQEIAKSEGRAPSHADDTAAETHAIARLLSGFSVEQLASEYRALRSSVLRLWAKEW 120 TQTEEQEIAKSEGRAP+HADDTAAETHAIARLLSGFSVEQLASEYRALRSSVLRLWAKEW Sbjct: 61 TQTEEQEIAKSEGRAPAHADDTAAETHAIARLLSGFSVEQLASEYRALRSSVLRLWAKEW 120 Query: 121 KEGYETNVDDITRFNEAIDQALAESLARYTKLLDQSRNLFLAILGHDLRNPLGATIHGAQ 180 KEGYETNVDDITRFNEAIDQALAESLARYTKLLDQSRNLFLAILGHDLRNPLGATIHGAQ Sbjct: 121 KEGYETNVDDITRFNEAIDQALAESLARYTKLLDQSRNLFLAILGHDLRNPLGATIHGAQ 180 Query: 181 IMLLAEDIDDRHRKIAARIYNSGQRMSKLVADLLDYTRTHLGSGLPLEISSANVGDIAQR 240 IMLLAEDIDDRHRKIAARIYNSGQRMSKLVADLLDYTRTHLGSGLPLEISSANVGDIAQR Sbjct: 181 IMLLAEDIDDRHRKIAARIYNSGQRMSKLVADLLDYTRTHLGSGLPLEISSANVGDIAQR 240 Query: 241 VIDEISTSHPDKVITLKTSGELNGEWDKERIAQAISNLLGNAVQHGATGEAIKVEISASR 300 VIDEISTSHPDKVITLKTSGELNGEWDKERIAQAISNLLGNAVQHGATGEAIKVEISASR Sbjct: 241 VIDEISTSHPDKVITLKTSGELNGEWDKERIAQAISNLLGNAVQHGATGEAIKVEISASR 300 Query: 301 GEVHVAINNRGPLIPEEKLHTIFDPLVRLVANRGEGGGEGSGQGGSMGLGLYIVREVASA 360 GEVHVAINN+GPLIPEEKLHTIFDPLVRLVAN GEG G+G G + YIVREVASA Sbjct: 301 GEVHVAINNKGPLIPEEKLHTIFDPLVRLVANGGEGSGQGGSMGLGL----YIVREVASA 356 Query: 361 HGGSVTASSSTAEGTTFTIRLPRDPSRRPADHQASPQVRHWEGDK 405 HGGSVTASSSTAEGTTFTIRLPRDPSRRPADHQASPQVRHWEGDK Sbjct: 357 HGGSVTASSSTAEGTTFTIRLPRDPSRRPADHQASPQVRHWEGDK 401 >gi|300312674|ref|YP_003776766.1| two-component system transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 372 Score = 177 bits (448), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 108/294 (36%), Positives = 161/294 (54%), Gaps = 24/294 (8%) Query: 91 RLLSGFSVEQLASEYRALRSSVLRLWAKEWKEGYETNVDDITRFNEAIDQALAESLARYT 150 RL G+ + Y+ + +LR A+ + + Y Q LA L T Sbjct: 101 RLFKGYESGAVDFLYKPIEPHILRNKAEVFFQLYRQK------------QQLALELRERT 148 Query: 151 KLLDQSRNLFLAILGHDLRNPLGATIHGAQIMLLAEDIDDRHRKIAARIYNSGQRMSKLV 210 + L + +F+AILGHDLRNPL A + A ++ ++ +K + +I N G+RM+KL+ Sbjct: 149 ETL-RLNEMFVAILGHDLRNPLSAVMMAATLLQRTYK-EEVAQKASQQILNGGKRMAKLI 206 Query: 211 ADLLDYTRTHLGSGLPLEISSANVGDIAQRVIDEISTSHPDKVITLKTSGELNGEWDKER 270 ++LD R L G+PL +SA +G+I +RV+ E ++P VI + G+ NG WD +R Sbjct: 207 ENMLDLARARLAGGIPLARASACLGEITERVVQEYQATYPGSVIERRIEGDDNGYWDGDR 266 Query: 271 IAQAISNLLGNAVQHGATGEAIKVEISA-SRGEVHVAINNRGPLIPEEKLHTIFDPLVRL 329 +AQ +NLLGNA+QHG G I + I S EV + I NRG +IP + L IFDP Sbjct: 267 LAQVAANLLGNALQHGEPGHPICIGIDGRSEHEVVMVIANRG-VIPADLLPVIFDPF--- 322 Query: 330 VANRGEGGGEGSGQGGSMGLGLYIVREVASAHGGSVTASSSTAEGTTFTIRLPR 383 GG +G+ +GLGL+I +++ AHGGS++ SS T T+RLPR Sbjct: 323 -----RGGEREAGRKEGLGLGLFIAQQIVKAHGGSISMSSDDQLQTMVTVRLPR 371 >gi|300311807|ref|YP_003775899.1| light-regulated signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 732 Score = 115 bits (287), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 75/234 (32%), Positives = 119/234 (50%), Gaps = 13/234 (5%) Query: 151 KLLDQSRNLFLAILGHDLRNPLGATIHGAQIMLLAEDIDDRHRKIAARIYNSGQRMSKLV 210 L+ ++R LA LGHDLR+PL A + A+++ + E ++ RI +S RM +L+ Sbjct: 506 NLVKRARETLLATLGHDLRDPLQAIMMAARMIEVREH-SPSSSNLSKRISSSSSRMHRLI 564 Query: 211 ADLLDYTRTHLGSGLPLEISSANVGDIAQRVIDEISTSHPDKVITLKT--SGELNGEWDK 268 + +LD +R G GL ++ +V + ++ E ++PD I L+ GE+ D Sbjct: 565 SQVLDISRLQSGMGLDIQRRPVDVRKMVSEIVSEARMAYPDNEIILEAEDCGEI--ALDG 622 Query: 269 ERIAQAISNLLGNAVQHGATGEAIKVEISASRGEVHVAINNRGPLIPEEKLHTIFDPLVR 328 +RI+Q +SNL+ N HG G+ + + + +A++N GP IPE +F P Sbjct: 623 DRISQVVSNLISNTRHHGEIGKPSTLIVFRREDVLTIAVSNHGPAIPEPVRSNLFKPY-- 680 Query: 329 LVANRGEGGGEGSGQGGSMGLGLYIVREVASAHGGSVTASSSTAEGTTFTIRLP 382 + E G G +GLGLYIV E+ HGG + S TFT+ LP Sbjct: 681 ----KKESLGNQRNTRG-LGLGLYIVSEIVHGHGGKIAVSCDN-NIITFTVTLP 728 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 84.0 bits (206), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 87/301 (28%), Positives = 151/301 (50%), Gaps = 29/301 (9%) Query: 99 EQLASEYRALRSSVLRLWAKEWKEGYETN--VDDITRFNEAIDQALAES----LARYTKL 152 E+L + +AL++S LRL ++ E ETN +++ T+ E LA++ + + T+L Sbjct: 410 EELEEQGQALKASQLRLETQQ-AELEETNAQLEEQTQLLELQKDDLAKTQVVLVEKATEL 468 Query: 153 --LDQSRNLFLAILGHDLRNPLGATIHGAQIMLLAED----IDDRHRKIAARIYNSGQRM 206 +Q ++ FLA + H+LR PL +T+ A+ LLA++ + D + A I ++G + Sbjct: 469 ERANQYKSEFLANMSHELRTPLNSTLILAK--LLADNKQGNLSDEQVRFAQTISSAGNDL 526 Query: 207 SKLVADLLDYTRTHLGSGLPLEISSANVGDIAQRVIDEISTSHPDKVITLKTSGE----L 262 L+ D+LD ++ G L + S + +A + + + K + K E Sbjct: 527 LALINDILDLSKMEAGK-LDIVPESFPLTKLADELSASFAITAQQKGLGFKVEVEPGVPQ 585 Query: 263 NGEWDKERIAQAISNLLGNAVQHGATGE-AIKVEISASRGEVHVAINNRGPLIPEEKLHT 321 E D +R+ Q + NLL NA++ GE ++++ A G + A+ + G I E++ Sbjct: 586 RMETDIQRLGQILKNLLSNAIKFTEKGEVSLRIYPDAG-GRIAFAVRDTGIGIGEDQHQF 644 Query: 322 IFDPLVRLVANRGEGGGEGSGQGGSMGLGLYIVREVASAHGGSVTASSSTAEGTTFTIRL 381 IF+ A R G GG+ GLGL I R++A GG + +S EG+ FT+ L Sbjct: 645 IFE------AFRQADGSTHRKYGGT-GLGLSISRDLAHLLGGDIRVTSRLGEGSIFTLSL 697 Query: 382 P 382 P Sbjct: 698 P 698 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 80.5 bits (197), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 71/249 (28%), Positives = 112/249 (44%), Gaps = 18/249 (7%) Query: 134 FNEAIDQALAESLARYTKLLDQSRNLFLAILGHDLRNPLGATIHGAQIMLLAEDIDDRHR 193 FN A+D+ + ++ YT+L F A + H+LR PL I Q+ L E + Sbjct: 231 FNGALDR-IEDA---YTQL-----EAFNADVAHELRTPLANLIGETQVALARERSAGEFK 281 Query: 194 KIAARIYNSGQRMSKLVADLLDYTRTHLGSGLPLEISSANVGDIAQRVIDEISTSHPDKV 253 + R+ +V D+L R+ G + A V D Q+ +D D Sbjct: 282 DVLQSNLEELDRIRSIVNDMLFLARSDQGEAATARVP-ARVADEVQKTVDFFEFVLDDSG 340 Query: 254 ITLKTSG--ELNGEWDKERIAQAISNLLGNAVQHGATGEAIKVEISASRGEVHVAINNRG 311 + ++ +G E + +A++NL+ NA+QH G ++++I+ V VA++N G Sbjct: 341 MRIEVAGDTEATAMMETALFKRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPG 400 Query: 312 PLIPEEKLHTIFDPLVRLVANRGEGGGEGSGQGGSMGLGLYIVREVASAHGGSVTASSST 371 I E L +FD R+ + R + G GLGL IV+ VA HGG ASS Sbjct: 401 KPIDEHHLPRLFDRFYRVDSARQDING-----SHGHGLGLAIVKAVARMHGGRAFASSKE 455 Query: 372 AEGTT-FTI 379 + FTI Sbjct: 456 GVNSIGFTI 464 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,185,877 Number of extensions: 45091 Number of successful extensions: 282 Number of sequences better than 1.0e-08: 33 Number of HSP's gapped: 246 Number of HSP's successfully gapped: 33 Length of query: 405 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 318 Effective length of database: 1,207,925 Effective search space: 384120150 Effective search space used: 384120150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)