BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0068.p (476 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312825|ref|YP_003776917.1| NAD-dependent aldehyde dehydrog... 958 0.0 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 238 6e-64 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 234 8e-63 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 232 4e-62 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 228 6e-61 >gi|300312825|ref|YP_003776917.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 476 Score = 958 bits (2477), Expect = 0.0, Method: Compositional matrix adjust. Identities = 475/476 (99%), Positives = 475/476 (99%) Query: 1 MHIASNIIGGQLRAGSRQRDSHNPATGAVMGRFPQSDAIEVAAAVAAARQAQPAWAALSL 60 MHIASNIIGGQLRAGSRQRDSHNPATGAVMGRFPQSDAIEVAAAVAAARQAQPAWAALSL Sbjct: 1 MHIASNIIGGQLRAGSRQRDSHNPATGAVMGRFPQSDAIEVAAAVAAARQAQPAWAALSL 60 Query: 61 VQRREYLNRVADAIAANAERLATLVTQEQGRPLGGVGPGQVPGSRFELWGCEAWTRATAS 120 VQRREYLNRVADAIAANAERLATLVTQEQGRPLGGVGP QVPGSRFELWGCEAWTRATAS Sbjct: 61 VQRREYLNRVADAIAANAERLATLVTQEQGRPLGGVGPDQVPGSRFELWGCEAWTRATAS 120 Query: 121 LELAPEVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPALYAGNTVVLKPSEY 180 LELAPEVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPALYAGNTVVLKPSEY Sbjct: 121 LELAPEVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPALYAGNTVVLKPSEY 180 Query: 181 TSVCGLELARVVADALPPGVLNTVSGDGSVGAMLVEDGDVNKIMFTGSGATGARIVAAAA 240 TSVCGLELARVVADALPPGVLNTVSGDGSVGAMLVEDGDVNKIMFTGSGATGARIVAAAA Sbjct: 181 TSVCGLELARVVADALPPGVLNTVSGDGSVGAMLVEDGDVNKIMFTGSGATGARIVAAAA 240 Query: 241 RNLTPTTMELGGNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIV 300 RNLTPTTMELGGNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIV Sbjct: 241 RNLTPTTMELGGNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIV 300 Query: 301 DELKAIIAALPMGDGLQPGIAMGPVQNPMQLKKVVALVESAREEGGTIVCGGVLPDGPGN 360 DELKAIIAALPMGDGLQPGIAMGPVQNPMQLKKVVALVESAREEGGTIVCGGVLPDGPGN Sbjct: 301 DELKAIIAALPMGDGLQPGIAMGPVQNPMQLKKVVALVESAREEGGTIVCGGVLPDGPGN 360 Query: 361 FYPLTLVTGLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDSQRA 420 FYPLTLVTGLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDSQRA Sbjct: 361 FYPLTLVTGLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDSQRA 420 Query: 421 AQVATRLQAGTVWVNQHGMVHPMVPFGGNKRSGWGVEFGQEGLKGVTQPQVISLKK 476 AQVATRLQAGTVWVNQHGMVHPMVPFGGNKRSGWGVEFGQEGLKGVTQPQVISLKK Sbjct: 421 AQVATRLQAGTVWVNQHGMVHPMVPFGGNKRSGWGVEFGQEGLKGVTQPQVISLKK 476 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 238 bits (608), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 152/462 (32%), Positives = 233/462 (50%), Gaps = 7/462 (1%) Query: 14 AGSRQRDSHNPATGAVMGRFPQSDAIEVAAAVAAARQAQPAWAALSLVQRREYLNRVADA 73 A R D NPAT V+G+ +D ++ A+ AA + W +S +R + + R AD Sbjct: 17 ASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERSKIMRRAADL 76 Query: 74 IAANAERLATLVTQEQGRPLGGVGPGQVPGSRFELWGCEAWTRATASLELAPEVVFEDET 133 + AE +A L+T EQG+PL + + W E R L P V Sbjct: 77 LRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYG--RLVPARV---PG 131 Query: 134 RRDELHRKPYGVIAAIAPWNWPLMISIWQVIPALYAGNTVVLKPSEYTSVCGLELARVVA 193 + ++P G +AA PWN+PL + ++ AL AG ++++K E T EL R A Sbjct: 132 AYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAYA 191 Query: 194 DA-LPPGVLNTVSG-DGSVGAMLVEDGDVNKIMFTGSGATGARIVAAAARNLTPTTMELG 251 DA +P GV+N V G + L+ + KI FTGS G ++ A A +++ TMELG Sbjct: 192 DAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRATMELG 251 Query: 252 GNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIVDELKAIIAALP 311 G+ A+V DDAD A L F N GQ C R V + + VD+ L Sbjct: 252 GHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTYAEQLK 311 Query: 312 MGDGLQPGIAMGPVQNPMQLKKVVALVESAREEGGTIVCGGVLPDGPGNFYPLTLVTGLR 371 +G+GL+ GI MGPV N ++ + AL++ A +G + GG G F+ T++T L Sbjct: 312 VGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPTVLTNLP 371 Query: 372 DGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDSQRAAQVATRLQAGT 431 AR ++EE FGP+ I + L++ I ANRL GL A ++ + +A R+++G Sbjct: 372 LQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAARVESGM 431 Query: 432 VWVNQHGMVHPMVPFGGNKRSGWGVEFGQEGLKGVTQPQVIS 473 + +N G+ P +PFGG K SG+G E G E ++ ++++ Sbjct: 432 LTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 234 bits (598), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 167/450 (37%), Positives = 226/450 (50%), Gaps = 20/450 (4%) Query: 6 NIIGGQLRAGSRQRDSHNPATGAVMGRFPQSDAIEVAAAVAAARQAQPAWAALSLVQRRE 65 + I G+ D +PA+G ++ Q A +V AAV AA+ A P W AL R Sbjct: 35 HFIAGRFTQPKADLDDIDPASGKLLAHLSQGSAADVDAAVQAAQAALPGWQALGGHGRAR 94 Query: 66 YLNRVADAIAANAERLATLVTQEQGRPLGGVGPGQVPGSRFELWGCEAWTRATASLELAP 125 +L +A + +A LA L T + G+PL VP + W A L+ Sbjct: 95 HLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGW----AQLQ--- 147 Query: 126 EVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPALYAGNTVVLKPSEYTSVCG 185 E F D P GV+ I PWN+PL++ W++ PAL GNTVVLKP+E TS+ Sbjct: 148 ESEFPDHV--------PVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTSLTA 199 Query: 186 LELARVVADA-LPPGVLNTVSGDGSVGAMLVEDGDVNKIMFTGSGATGARIVAAAARNLT 244 L A + A LP GVLN V+GDG+ GA +V + KI FTGS G I A + Sbjct: 200 LLFAELAQQAGLPAGVLNIVTGDGATGAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSGK 259 Query: 245 PTTMELGGNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIVDELK 304 T+ELGG IV +DAD A G+ + N GQ C RL V + +HD + LK Sbjct: 260 SLTLELGGKSPFIVFEDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARLK 319 Query: 305 AIIAALPMGDGLQPGIAMGPVQNPMQLKKVVALVESAREEGGTIVCGGVLPDGP--GNFY 362 + L +G L MG + +P+QL++V +LVE EG C V+ D P G FY Sbjct: 320 TRMQKLKVGAPLDKCSDMGALISPVQLERVRSLVEQGVREGAQ--CHQVVLDTPPGGCFY 377 Query: 363 PLTLVTGLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDSQRAAQ 422 P TL+TG+ A + EE FGPVL + + ++A+ AN GL AS+WS A Sbjct: 378 PPTLLTGVHPAATVASEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASIWSETIGLALG 437 Query: 423 VATRLQAGTVWVNQHGMVHPMVPFGGNKRS 452 VA +L+AG VW+N V FGG + S Sbjct: 438 VAPQLKAGVVWINSTNQFDAAVGFGGVRES 467 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 232 bits (592), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 150/464 (32%), Positives = 235/464 (50%), Gaps = 17/464 (3%) Query: 6 NIIGGQLRAGSRQRDSHN--PATGAVMGRFPQSDAIEVAAAVAAARQAQPAWAALSLVQR 63 N IGG+ + N P G +S A +V A+ AA A+ +W S +R Sbjct: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTSPTER 80 Query: 64 REYLNRVADAIAANAERLATLVTQEQGRPLGGVGPGQVPGSRFELWGCEAWTRATASLEL 123 L ++AD + AN E LAT T + G+P+ +P + A R T + Sbjct: 81 ANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVR-TQEGSI 139 Query: 124 APEVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPALYAGNTVVLKPSEYTSV 183 P +++T H +P GV+ I PWN+P+++++W++ PAL AGN VVLKP+E T Sbjct: 140 CP---IDNDTYAYHFH-EPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPA 195 Query: 184 CGLELARVVADALPPGVLNTVSGDG-SVGAMLVEDGDVNKIMFTGSGATGARIVAAAARN 242 + L ++AD +PPGV+N V G G G L + + KI FTG TG I+ A++N Sbjct: 196 SIMVLIELIADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQN 255 Query: 243 LTPTTMELGGNDAAIVLDDA-----DPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHD 297 L P T+ELGG I D D A F LN G+ C C R+ V +++++ Sbjct: 256 LIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLVQESIYE 315 Query: 298 QIVDELKAIIAALPMGDGLQPGIAMGPVQNPMQLKKVVALVESAREEGGTIVCGGVLPDG 357 + ++ +AA+ G+ L +G + QL+K+++ ++ ++EG ++ GG + Sbjct: 316 RFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREEL 375 Query: 358 PGN----FYPLTLVTGLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVW 413 G+ +Y V + R+ EE FGPV+ + + D E+A+A AN GLGA +W Sbjct: 376 GGDLASGYYVKPTVFQGNNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLW 435 Query: 414 SSDSQRAAQVATRLQAGTVWVNQHGMVHPMVPFGGNKRSGWGVE 457 + D RA ++ +QAG VW N + + FGG K+SG G E Sbjct: 436 TRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRE 479 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 228 bits (582), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 172/470 (36%), Positives = 239/470 (50%), Gaps = 16/470 (3%) Query: 5 SNIIGGQLR--AGSRQRDSHNPATGAVMGRFPQSDAIEVAAAVAAARQAQPAWAALSLVQ 62 + +IG R A RQ D +PATG V P A + AAV AA A AW A Q Sbjct: 15 AQLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAWRATPAKQ 74 Query: 63 RREYLNRVADAIAANAERLATLVTQEQGRPLGGVGPGQVPGSRFELWGCEAWTRATASLE 122 R + R D + A+ + L L+++EQG+PL + + W E TRA + Sbjct: 75 RAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDII 134 Query: 123 LAPEVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPALYAGNTVVLKPSEYTS 182 AP RR ++P GV+AAI PWN+P + ++ PAL AG TVV KP+E T Sbjct: 135 PAPVT-----GRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTP 189 Query: 183 VCGLELARVVADA-LPPGVLNTVSGDGSVGAMLVE----DGDVNKIMFTGSGATGARIVA 237 + L L R+ +A +P GV+N V+ +V+ DG V KI FTGS A G + Sbjct: 190 LTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLAR 249 Query: 238 AAARNLTPTTMELGGNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHD 297 +A L ++ELGGN IV DDAD A G+ F N GQTC R+YV + ++D Sbjct: 250 HSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYD 309 Query: 298 QIVDELKAIIAALPMGDGLQPGIAMGPVQNPMQLKKVVALVESAREEGGTIVCGGVLPDG 357 VD+L A +AAL +G P +GP+ N + K+ V A G ++ GG G Sbjct: 310 AFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQG 369 Query: 358 PG----NFYPLTLVTGLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVW 413 PG N+Y T++ + + EE FGPV PI R++ ++ IA AN GL A + Sbjct: 370 PGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFY 429 Query: 414 SSDSQRAAQVATRLQAGTVWVNQHGMVHPMVPFGGNKRSGWGVEFGQEGL 463 S+D +R +V L++G V VN+ + PFGG K SG+G E GL Sbjct: 430 STDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGL 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,478,227 Number of extensions: 60330 Number of successful extensions: 272 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 20 Length of query: 476 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 387 Effective length of database: 1,198,455 Effective search space: 463802085 Effective search space used: 463802085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)