BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0060.p (680 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312832|ref|YP_003776924.1| flagellar capping protein [Herb... 1317 0.0 gi|300310454|ref|YP_003774546.1| flagellar hook-associated prote... 761 0.0 gi|300311383|ref|YP_003775475.1| flagellar hook-associated 2 pro... 728 0.0 >gi|300312832|ref|YP_003776924.1| flagellar capping protein [Herbaspirillum seropedicae SmR1] Length = 680 Score = 1317 bits (3409), Expect = 0.0, Method: Compositional matrix adjust. Identities = 673/680 (98%), Positives = 676/680 (99%) Query: 1 MATSTGSISTSAGPLDVANLVNQLVSVESKSRLTPLASKADSFNALISAYGKLGTALTTY 60 MATSTGSISTSAGPLDVANLVNQLVSVESKSRLTPLASKADSFNALISAYGKLGTALTTY Sbjct: 1 MATSTGSISTSAGPLDVANLVNQLVSVESKSRLTPLASKADSFNALISAYGKLGTALTTY 60 Query: 61 QNALAELTAAKFSAQKAAVSNTGRSSDGKSGNDAVSADINTDASTKAQAQIIQSAGFASG 120 QNALAELTAAKFSAQKAAVSNTGRSSDGKSGNDAVSADIN+DASTKAQAQII+SAGFASG Sbjct: 61 QNALAELTAAKFSAQKAAVSNTGRSSDGKSGNDAVSADINSDASTKAQAQIIKSAGFASG 120 Query: 121 HLFKAGDSVAIKVGEASPSFITLKADATINGLRDTINAARAGVTASVVRDDAGDHLVLES 180 HLFKAGDSVAIKVGEASP FITLKADATINGLRDTINAARAGVTASVVRDDAGDHLVLES Sbjct: 121 HLFKAGDSVAIKVGEASPHFITLKADATINGLRDTINAARAGVTASVVRDDAGDHLVLES 180 Query: 181 NTAGTSKLLKVRANNSLSAISFDAQQSRPGAMTQTQAARDATSAAPGSYAISVEQLAQSH 240 NTAGT+ LLKVRANNSLSAISFDAQQSRPGAMTQTQAARDATSAAPGSYAISVEQLAQSH Sbjct: 181 NTAGTANLLKVRANNSLSAISFDAQQSRPGAMTQTQAARDATSAAPGSYAISVEQLAQSH 240 Query: 241 KLSSAGIAPGSTFDLGVLAIKAGSGSTTLIKPASNTLAGVRDAINAAAAGVNASIVSDGK 300 KLSSAGIAPGSTFDLGVLAIKAGSGSTTLIKPASNTLAGVRDAINAAAAGVNASIVSDGK Sbjct: 241 KLSSAGIAPGSTFDLGVLAIKAGSGSTTLIKPASNTLAGVRDAINAAAAGVNASIVSDGK 300 Query: 301 QEHLVLTAKESGANNALRISGTGDFAVFSSDPSGNASTASVTPEKRFSQGAVNLSVGART 360 QEHLVLTAKESGANNALRISGTGDFAVFSSDPSGNASTASVTPEKRFSQGAVNLSVGART Sbjct: 301 QEHLVLTAKESGANNALRISGTGDFAVFSSDPSGNASTASVTPEKRFSQGAVNLSVGART 360 Query: 361 ISLKPASATAGAAPALADVARAINEAKAGVSATVQREGQGDKAVERLVLSASGSDPVSLR 420 ISLKPASATAGAAP LADVARAINEAKAGVSATVQREGQGDKAVERLVLSASGSDPVSLR Sbjct: 361 ISLKPASATAGAAPGLADVARAINEAKAGVSATVQREGQGDKAVERLVLSASGSDPVSLR 420 Query: 421 GSGDYAVLAASAMGQLQKPQDARLSIDGVAITATSNTIKDAISGVTLNLNKTTSATDKLT 480 GSGDYAVLAASAMGQLQKPQDARLSIDGVAITATSNTIKDAISGVTLNLNKTTSATDKLT Sbjct: 421 GSGDYAVLAASAMGQLQKPQDARLSIDGVAITATSNTIKDAISGVTLNLNKTTSATDKLT 480 Query: 481 LAISNDSSGVGAAVNSFVNAFNTLAKAMAEMTRQAPAKVRGQAGEQGPLAGESMVRALTG 540 LAISNDSSGVGAAVNSFVNAFNTLAKAMAEMTRQAPAKVRGQAGEQG LAGESMVRALTG Sbjct: 481 LAISNDSSGVGAAVNSFVNAFNTLAKAMAEMTRQAPAKVRGQAGEQGLLAGESMVRALTG 540 Query: 541 ELRNAVLASMPGNALGSLTAIGVGFKKDGTLDLDSARLAKAGERNFEAVAQLFAGKEGLV 600 ELRNAVLASMPGNALGSLTAIGVGFKKDGTLDLDSARLAKAGERNFEAVAQLFAGKEGLV Sbjct: 541 ELRNAVLASMPGNALGSLTAIGVGFKKDGTLDLDSARLAKAGERNFEAVAQLFAGKEGLV 600 Query: 601 ARTRSLLDKVLGEQGLLAGKTRGLQGSLKIVSDQQAAARARLANLRDSYTNQFNRLNVTL 660 ARTRSLLDKVLGEQGLLAGKTRGLQGSLKIVSDQQAAARARLANLRDSYTNQFNRLNVTL Sbjct: 601 ARTRSLLDKVLGEQGLLAGKTRGLQGSLKIVSDQQAAARARLANLRDSYTNQFNRLNVTL 660 Query: 661 SKMQASQNYLTQQLARLRRP 680 SKMQASQNYLTQQLARLRRP Sbjct: 661 SKMQASQNYLTQQLARLRRP 680 >gi|300310454|ref|YP_003774546.1| flagellar hook-associated protein 2 [Herbaspirillum seropedicae SmR1] Length = 676 Score = 761 bits (1964), Expect = 0.0, Method: Compositional matrix adjust. Identities = 386/680 (56%), Positives = 509/680 (74%), Gaps = 7/680 (1%) Query: 1 MATSTGSISTSAGPLDVANLVNQLVSVESKSRLTPLASKADSFNALISAYGKLGTALTTY 60 MATSTG+ISTSAGPLDVA LVNQL+SVESKSRLTPL +K SFN LISAYG L A T+Y Sbjct: 1 MATSTGTISTSAGPLDVATLVNQLISVESKSRLTPLKNKESSFNTLISAYGSLKNAFTSY 60 Query: 61 QNALAELTAAKFSAQKAAVSNTGRSSDGKSGNDAVSADINTDASTKAQAQIIQSAGFASG 120 Q+AL LTAA FSAQK +SN G ++ + D +AD NTD STK AQ +QSAGF +G Sbjct: 61 QSALKTLTAASFSAQKTTLSNAGTGTNLTT--DPFTADTNTDESTKVLAQKLQSAGFTAG 118 Query: 121 HLFKAGDSVAIKVGEASPSFITLKADATINGLRDTINAARAGVTASVVRDDAGDHLVLES 180 F AGDSVAIKVG P+FITL+A+AT+ G+RD INAA+AGVTAS+ D +GDHLVLES Sbjct: 119 QTFNAGDSVAIKVGTNQPTFITLQANATLAGVRDAINAAKAGVTASITTDGSGDHLVLES 178 Query: 181 NTAGTSKLLKVRANNSLSAISFDAQQSRPGAMTQTQAARDATSAAPGSYAISVEQLAQSH 240 NT GT+ +K+ ANNSLS++++D + +TQ QA RDA+ AA G Y I+V QLAQ+ Sbjct: 179 NTGGTANTIKITANNSLSSLAYDPSSNVSSTVTQIQAPRDASKAAAGKYNIAVNQLAQAV 238 Query: 241 KLSSAGIAPGSTFDLGVLAIKAGSGSTTLIKPASNTLAGVRDAINAAAAGVNASIVSDGK 300 K+SSAGIAPGSTFD GVLAIK G+GST +I+P +N+LAGVRDAINA+ AGVNASIV+DG Sbjct: 239 KVSSAGIAPGSTFDNGVLAIKTGNGSTAIIQPKTNSLAGVRDAINASDAGVNASIVNDGT 298 Query: 301 QEHLVLTAKESGANNALRISGTGDFAVFSSDPSGNASTASVTPEKRFSQGAVNLSVGART 360 +HLVLTAK++G+ N LR++GTG+FA F+ DPSG +T V + +S G++ L VG+++ Sbjct: 299 NDHLVLTAKDAGSANTLRVTGTGNFAAFNFDPSGTITTTGVATNQTYSSGSLALQVGSKS 358 Query: 361 ISLKPASATAGAAPALADVARAINEAKAGVSATVQREGQGDKAVERLVLSASGSDPVSLR 420 ++ P A L DV +AIN+A GV+A++ +G D LVL+ SG+D V L Sbjct: 359 FTINPTDLDGSGAIGLNDVMKAINDANTGVTASITNDGSKD----HLVLTPSGTDAVKLT 414 Query: 421 GSGDYAVLAASAMGQLQKPQDARLSIDGVAITATSNTIKDAISGVTLNLNKTTSATDKLT 480 G+GDY+ LA S+MGQL K QDA+L++DGV +T+TSN + + ISGVTLNL+K T++ D + Sbjct: 415 GAGDYSDLAGSSMGQLAKAQDAKLTVDGVTVTSTSNKVTNVISGVTLNLSKVTTSADNFS 474 Query: 481 LAISNDSSGVGAAVNSFVNAFNTLAKAMAEMTRQAPAKVRGQAGEQGPLAGESMVRALTG 540 L+I+ND+SG+ A N+FV A+NTLAKA+A +T+Q P+ +GQA PLA ES V AL G Sbjct: 475 LSINNDTSGLSTAANTFVTAYNTLAKAVANLTKQTPSTTKGQASTGSPLASESSVLALMG 534 Query: 541 ELRNAVLASMPGNALGSLTAIGVGFKKDGTLDLDSARLAKAGERNFEAVAQLF-AGKEGL 599 ++RN +L ++ N +L+ IG+ F+KDGTL LD+ +L A ++F+ + QLF + G+ Sbjct: 535 QVRNTMLGALGDNGATNLSQIGISFQKDGTLALDATKLTNAANKDFDGITQLFTSATNGV 594 Query: 600 VARTRSLLDKVLGEQGLLAGKTRGLQGSLKIVSDQQAAARARLANLRDSYTNQFNRLNVT 659 V + + L+D +LGE G+LA KT+GLQ SLKIV+DQQ AA ARL +L+D+YTNQFNRLNVT Sbjct: 595 VPKLQKLMDGILGESGVLASKTKGLQDSLKIVTDQQTAANARLQDLKDTYTNQFNRLNVT 654 Query: 660 LSKMQASQNYLTQQLARLRR 679 L+ MQ+ Q+YLTQQLA+L + Sbjct: 655 LATMQSRQSYLTQQLAKLSK 674 >gi|300311383|ref|YP_003775475.1| flagellar hook-associated 2 protein [Herbaspirillum seropedicae SmR1] Length = 690 Score = 728 bits (1880), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/694 (55%), Positives = 504/694 (72%), Gaps = 22/694 (3%) Query: 1 MATSTGSISTSAGPLDVANLVNQLVSVESKSRLTPLASKADSFNALISAYGKLGTALTTY 60 MATSTG+ISTSAGPLDVA LV+QL+S ES+S+LTPL ++A S+N LISAYG L +A++TY Sbjct: 1 MATSTGTISTSAGPLDVATLVSQLISAESQSQLTPLTTQASSYNTLISAYGSLKSAMSTY 60 Query: 61 QNALAELTAAKFSAQKAAVSNTGRSSDGKSGNDAVSADINTDASTKAQAQIIQSAGFASG 120 Q+A+ +T+A FSAQK++VSN G S+ + D SADIN D STK AQ IQSAG +SG Sbjct: 61 QSAIKAMTSASFSAQKSSVSNAGTGSNLTT--DPFSADINGDDSTKILAQKIQSAGISSG 118 Query: 121 HLFKAGDSVAIKVGEASPSFITLKADATINGLRDTINAARAGVTASVVRDDAGDHLVLES 180 F AGDS+AIK+G SP F+TL+A++T+ G+RD INA + G TAS+V D AGDHLVLES Sbjct: 119 TTFNAGDSIAIKIGTNSPVFVTLQANSTLAGVRDAINAGKTGATASIVTDGAGDHLVLES 178 Query: 181 NTAGTSKLLKVRANNSLSAISFDAQQSRPGAMTQTQAARDATSAAPGSYAISVEQLAQSH 240 NT GT+ +KV ANNSLS +D S P MTQTQA RDAT AA G+YA++V QLAQ+ Sbjct: 179 NTGGTANTIKVTANNSLSGFFYDPSSSTPSTMTQTQAPRDATKAASGNYAVAVSQLAQAE 238 Query: 241 KLSSAGIAPGSTFDLGVLAIKAGSGSTTLIKPASNTLAGVRDAINAAAAGVNASIVSDGK 300 KLSSA IAPGSTFD G+LAIK GSGSTT+IKP +NTLAGVRDAIN++ AGVNA+IVSDG Sbjct: 239 KLSSASIAPGSTFDNGLLAIKTGSGSTTIIKPTTNTLAGVRDAINSSDAGVNATIVSDGT 298 Query: 301 QEHLVLTAKESGANNALRISGTGDFAVFSSDPSGNASTASVTPEKRFSQGAVNLSVGART 360 +HLV+TAK SG+ N ++I+GT +FAVF+SDPSG ++ +V + +S G + L VG + Sbjct: 299 NDHLVVTAKNSGSTNTIKITGTENFAVFNSDPSGTVTSPNVASGQTYSAGNLTLKVGDSS 358 Query: 361 ISLKPASATAGAAPALADVARAINEAKAGVSATVQREGQGDKAVERLVLSASGSD---PV 417 ++ P + +G L V AIN A AGV+A++ +G D LVL+ +GS V Sbjct: 359 FNIAPVANGSGNID-LTQVKLAINNANAGVTASITNDGSND----HLVLTPTGSSATAAV 413 Query: 418 SLRGSGDYAVLAASAMGQLQKPQDARLSIDGVAITATSNTIKDAISGVTLNLNKTTSATD 477 SL G+ DYA L+ S MGQL K QDA+LSIDGV +T+ +N +++ ISGVTLNL+K T++ D Sbjct: 414 SLTGTSDYAGLSMSGMGQLLKAQDAKLSIDGVNVTSPTNKVENVISGVTLNLSKVTTSAD 473 Query: 478 KLTLAISNDSSGVGAAVNSFVNAFNTLAKAMAEMTRQAPAKVRGQAGEQGPLAGESMVRA 537 TL ISND+SG+ A NSFV A+NTLAKA+A MT Q P+ GQA PLA ES V+ Sbjct: 474 NFTLNISNDTSGITTAANSFVTAYNTLAKAVAGMTAQTPSTTLGQANTSAPLASESSVQT 533 Query: 538 LTGELRNAVLASMP-GNALGSLTAIGVGFKKDGTLDLDSARLAKAGERNFEAVAQLFAG- 595 + +LRN + +++ GN + +L+ IG+ F+KDGTL LDS +L+ A NF +A LFAG Sbjct: 534 IMSQLRNTLFSTVDGGNGINTLSDIGISFQKDGTLALDSTKLSTAATNNFAGIANLFAGT 593 Query: 596 ----------KEGLVARTRSLLDKVLGEQGLLAGKTRGLQGSLKIVSDQQAAARARLANL 645 K G+V + ++L+ +L + G++A KT GLQ SLKI D+Q A ++RL+NL Sbjct: 594 DPDPTNTTSSKNGIVTKLQTLMTSLLSDNGIIATKTSGLQASLKINQDRQTAVQSRLSNL 653 Query: 646 RDSYTNQFNRLNVTLSKMQASQNYLTQQLARLRR 679 +D+YTNQFN+LN+TLS MQ++Q+YLTQQLA L + Sbjct: 654 KDAYTNQFNQLNLTLSSMQSTQSYLTQQLANLAK 687 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.309 0.123 0.319 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,719,430 Number of extensions: 66371 Number of successful extensions: 314 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 283 Number of HSP's successfully gapped: 3 Length of query: 680 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 588 Effective length of database: 1,184,250 Effective search space: 696339000 Effective search space used: 696339000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 134 (56.2 bits)