BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0059.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbasp... 828 0.0 gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protei... 584 e-168 gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protei... 75 8e-15 >gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbaspirillum seropedicae SmR1] Length = 420 Score = 828 bits (2140), Expect = 0.0, Method: Compositional matrix adjust. Identities = 417/420 (99%), Positives = 418/420 (99%) Query: 1 MSFQQGLSGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSPVGIGVT 60 MSFQQGLSGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSPVGIGVT Sbjct: 1 MSFQQGLSGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSPVGIGVT 60 Query: 61 VANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGYLINP 120 VANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGYLINP Sbjct: 61 VANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGYLINP 120 Query: 121 GMNGAYLTGYPAGTNGGDPVPLKIDTATLPATATTRVSTKVNLDSRKSVPTVSPFDPANP 180 MNGAYLTGYPAGTNGGDPVPLKIDTATLPATATTRVSTKVNLDSRKSVPTVSPFDPANP Sbjct: 121 SMNGAYLTGYPAGTNGGDPVPLKIDTATLPATATTRVSTKVNLDSRKSVPTVSPFDPANP 180 Query: 181 NSYSNSTGATVYDSLGNPYSLQTFYVKGAATGTPPSTTWSVYATLDGKTIINAPPATGPQ 240 NSYSNSTGATVYDSLGNPYSLQTFYVKGAATGTPPSTTWSVYATLDGKTIINAPPATGPQ Sbjct: 181 NSYSNSTGATVYDSLGNPYSLQTFYVKGAATGTPPSTTWSVYATLDGKTIINAPPATGPQ 240 Query: 241 LLGTLRFDATGALTASTGMSLNLSTLAKGGATFPGPIALGYAGSTQTGSAFANLSQSQDG 300 LLGTLRFDATGALTASTGMSLNLSTLAKGGATFPGPIALGYAGSTQTGSAFANL+QSQDG Sbjct: 241 LLGTLRFDATGALTASTGMSLNLSTLAKGGATFPGPIALGYAGSTQTGSAFANLAQSQDG 300 Query: 301 MPPGTLSSFSIGKDGSIIGAYSNQQTRVMGQIVLVNFANPHGLQPIGNNLYQASAAAGSA 360 MPPGTLSSFSIGKDGSIIGAYSNQQTRVMGQIVLVNFANPHGLQPIGNNLYQAS AAGSA Sbjct: 301 MPPGTLSSFSIGKDGSIIGAYSNQQTRVMGQIVLVNFANPHGLQPIGNNLYQASEAAGSA 360 Query: 361 LVGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQTLVSLR 420 LVGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQTLVSLR Sbjct: 361 LVGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQTLVSLR 420 >gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protein FlgE [Herbaspirillum seropedicae SmR1] Length = 432 Score = 584 bits (1505), Expect = e-168, Method: Compositional matrix adjust. Identities = 302/432 (69%), Positives = 357/432 (82%), Gaps = 12/432 (2%) Query: 1 MSFQQGLSGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSPVGIGVT 60 M FQQGLSGL+GA+K LDVIGNNVANASTVGFKQ Q QFAD YANSMNRSGNSPVGIGV+ Sbjct: 1 MGFQQGLSGLNGAAKSLDVIGNNVANASTVGFKQSQAQFADMYANSMNRSGNSPVGIGVS 60 Query: 61 VANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGYLINP 120 V V+Q FTQGNI+++NN LD+AINGDGFF+++ASLT++SP YGRNGQFQL+K GY++NP Sbjct: 61 VPAVAQQFTQGNISSSNNPLDIAINGDGFFQLSASLTNKSPMYGRNGQFQLDKSGYIVNP 120 Query: 121 GMNGAYLTGYPAGTNGGDPVPLKIDTATLPATATTRVSTKVNLDSRKSVPTVSPFDPANP 180 MNGAYL G+ AG GGDPVPL+IDT+++PAT TT +STKVNLDSR SVPT +PF+ A+P Sbjct: 121 SMNGAYLMGWAAGAQGGDPVPLQIDTSSIPATPTTTISTKVNLDSRLSVPTTTPFNAADP 180 Query: 181 NSYSNSTGATVYDSLGNPYSLQTFYVKGAATGTPPSTTWSVYATLDGKTIINAPPATGPQ 240 SY++STG +YDSLGNPYS+QT+YVK A TGTPP+TTW+VYA +D T+ N P Sbjct: 181 TSYNSSTGVDLYDSLGNPYSMQTYYVKAAPTGTPPTTTWTVYAKIDNTTLNNGATPAVPV 240 Query: 241 LLGTLRFDATGALTASTGMSLN------------LSTLAKGGATFPGPIALGYAGSTQTG 288 LGT+ FD+TG +T G +++ L+T+AK G F PI + Y+GSTQTG Sbjct: 241 ALGTMTFDSTGTMTQINGAAISPTNNTLTISTAQLATVAKAGGVFAAPINISYSGSTQTG 300 Query: 289 SAFANLSQSQDGMPPGTLSSFSIGKDGSIIGAYSNQQTRVMGQIVLVNFANPHGLQPIGN 348 SAF NL+Q Q+GMPPG+LSSFS+ KDGSIIG+YSNQQT+ +G +VLVNFANP+GLQP+GN Sbjct: 301 SAFVNLAQVQNGMPPGSLSSFSVDKDGSIIGSYSNQQTKNLGTVVLVNFANPNGLQPLGN 360 Query: 349 NLYQASAAAGSALVGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKV 408 NLYQAS+AAGS LVG PTTG FG LQ RA E SNVDLTAELVNMIVAQRVYQANSQTIKV Sbjct: 361 NLYQASSAAGSPLVGKPTTGTFGTLQARAKEDSNVDLTAELVNMIVAQRVYQANSQTIKV 420 Query: 409 QDTVLQTLVSLR 420 QDTVLQTLVSLR Sbjct: 421 QDTVLQTLVSLR 432 >gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protein FlgG [Herbaspirillum seropedicae SmR1] Length = 260 Score = 75.5 bits (184), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 51/124 (41%), Positives = 69/124 (55%), Gaps = 7/124 (5%) Query: 302 PPGTL----SSFSIGKDG--SIIGAYSNQQTRVMGQIVLVNFANPHGLQPIGNNLYQASA 355 PP T+ + ++G+DG SI+ A S T++ G I + F NP GL+ G NLY +A Sbjct: 138 PPITVPLNTTKLTVGRDGVVSIMQAGSTATTQI-GTIQVATFINPAGLESRGENLYMETA 196 Query: 356 AAGSALVGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQT 415 A+G+ P T GAL VE SNV++ ELVNMI QR Y+ NS+ I D +L Sbjct: 197 ASGAPNPNNPGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAYEINSKAITTSDQMLAK 256 Query: 416 LVSL 419 L L Sbjct: 257 LSQL 260 Score = 75.1 bits (183), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 45/122 (36%), Positives = 63/122 (51%), Gaps = 13/122 (10%) Query: 8 SGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSP-----------VG 56 +GL LDVI NN+AN ST GFK+ + F D ++ + G +G Sbjct: 10 TGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQTQLPSGLQIG 69 Query: 57 IGVTVANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGY 116 GV + FTQGN+ TNN D+AI G+GFF++ L D + Y R+G FQ + G Sbjct: 70 TGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQIL--LPDGTTAYTRDGAFQTDSQGQ 127 Query: 117 LI 118 L+ Sbjct: 128 LV 129 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.128 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,373,084 Number of extensions: 62520 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 172 Number of HSP's successfully gapped: 4 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)