BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0038.p (324 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 657 0.0 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 142 4e-35 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 90 2e-19 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 89 4e-19 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 80 2e-16 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 657 bits (1695), Expect = 0.0, Method: Compositional matrix adjust. Identities = 323/324 (99%), Positives = 323/324 (99%) Query: 1 MAKAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYPQPLPAGL 60 MAKAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYPQPLPAGL Sbjct: 1 MAKAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYPQPLPAGL 60 Query: 61 GQEAAGTIEAVGAGVSGFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTAAAM 120 GQEAAGTIEAVGAGVSGFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTAAAM Sbjct: 61 GQEAAGTIEAVGAGVSGFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTAAAM 120 Query: 121 MLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEKAALA 180 MLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEKAALA Sbjct: 121 MLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEKAALA 180 Query: 181 LQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVSFGNA 240 LQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVSFGNA Sbjct: 181 LQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVSFGNA 240 Query: 241 SGPVAPFGVSELASRGSLFLTRPSLGNYTSTRAELDATAGDLFDMVLGNKISIEINQRYA 300 SGPVAPFGVSELASRGSLFLTRPSLGNYTSTRAELDATAGDLFDMVLGNKISIEINQRYA Sbjct: 241 SGPVAPFGVSELASRGSLFLTRPSLGNYTSTRAELDATAGDLFDMVLGNKISIEINQRYA 300 Query: 301 LKDVGQAHADLEGRKTTGSTILIP 324 LKDV QAHADLEGRKTTGSTILIP Sbjct: 301 LKDVAQAHADLEGRKTTGSTILIP 324 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 142 bits (358), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 115/331 (34%), Positives = 158/331 (47%), Gaps = 20/331 (6%) Query: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYPQP-----LP 57 +AI + + GGPEV++ + V E AGEV I+ A G+N D + R G YP P LP Sbjct: 2 RAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDLP 61 Query: 58 AGLGQEAAGTIEAVGAGVSGFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTA 117 G E AG I A G SGFK+GD V A G YAE P + +P +S A Sbjct: 62 ---GLEVAGEIVAGDLGDSGFKLGDLVC-ALVQGGGYAEFCAAPLAQCLPVPKGLSALEA 117 Query: 118 AAMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEKA 177 AA+ TV + + +T+L + GIG+ A Q ARALG + T S EK Sbjct: 118 AALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKC 177 Query: 178 ALALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVSF 237 + GA INYKTE+F VKELTGGRGV V+ D +G D + L G +V Sbjct: 178 RACEELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGRLVFI 237 Query: 238 GNASGPVAPFGVSELASR-----GSLFLTRPSLGNYTSTRAELDATAGD-LFDMVLGNKI 291 G + ++ R GS RP + +A + A + ++ ++ KI Sbjct: 238 AQLGGAKGQLDMGQVMRRRLTITGSTLRPRP-----VAFKAAIAAKLREHVWPLIEAGKI 292 Query: 292 SIEINQRYALKDVGQAHADLEGRKTTGSTIL 322 I+ R+ L+ QAHA +EG G +L Sbjct: 293 RPVIHHRFPLEQAAQAHAMMEGSTHIGKIML 323 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 90.1 bits (222), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 86/301 (28%), Positives = 140/301 (46%), Gaps = 44/301 (14%) Query: 1 MAKAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYP-QPLPAG 59 M KA + + G P +E V V P G++ ++ A G+ D + G +P +P P Sbjct: 4 MMKAAVVREFGKPLSIEQVPVPT--PAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPF 61 Query: 60 L-GQEAAGTIEAVGAGVSGFKVGDRVAYAGRP---------------------------- 90 + G E G + AVGAGV K GDRV G P Sbjct: 62 IPGHEGTGYVAAVGAGVKHVKEGDRV---GVPWLHTACGCCSPCRTGWETLCAEQQNTGY 118 Query: 91 --NGAYAEERVMPADILVKLPDAISFDTAAAMMLQGMTVQYLLRQTYAVQPGDTILFHAA 148 NG++AE + + LPD + F AA ++ G+TV L++T V+PG+ ++ + Sbjct: 119 SVNGSFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKET-EVRPGEWVVI-SG 176 Query: 149 AGGIGLIACQWARALGVNLIGTVSSDEKAALALQHGAAHVINYKTENFVERVKELTGGRG 208 GG+G +A Q+A+A+G++++ ++K ALA + GA ++ + N V V+ + GG Sbjct: 177 IGGLGHMAVQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGGAH 236 Query: 209 VQVVYDSIGKDTFLGSLDCLRPRGMM----VSFGNASGPVAPFGVSELASRGSLFLTRPS 264 +V ++ + LR RG M + G+ S PV + + RGS+ TR Sbjct: 237 GALV-TAVSPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRITVRGSIVGTRQD 295 Query: 265 L 265 L Sbjct: 296 L 296 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 109/337 (32%), Positives = 151/337 (44%), Gaps = 52/337 (15%) Query: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAG---LYP--QP-- 55 KAI+ GGPEV+ Y D V E GEV +R AVGLN D Y R G L P QP Sbjct: 7 KAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQPTV 66 Query: 56 -LPAGLGQEAAGTIEAVGAGVSGFKVGDRVAYAGR-PNG------AYAEERVMPADILVK 107 P LG + +G +EAV V F VGD V R P+G AYA+ + A + Sbjct: 67 AFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEIAL 126 Query: 108 LPDAISFDTAAAMMLQGMTV-QYLLRQTYAVQ-------------PGDTILFHAAAGGIG 153 P IS AAA + +T Q+++ + +Q T++ + AAGG+G Sbjct: 127 KPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAGGVG 186 Query: 154 LIACQWARALGVNLIGTVSSDEKAALALQHGAAHVINYKTENFVERVKELTGGRGVQVVY 213 A Q A+ G +I S +A L GA I+Y T + E V V +V Sbjct: 187 HFALQLAKWKGARVIAIGSGGHEAFLR-DLGADEFIDY-TRSAAEDVLS-----DVDLVI 239 Query: 214 DSIGKDTFLGSLDCLRPRGMMVSFGNASGPVAPFGVS--ELASRGSLFLTRPSLGNYTST 271 D++G L L+ G A P+ P G S E A+R + + S S+ Sbjct: 240 DAVGGPASGRFLRTLK-------RGGALFPIFPLGFSGAEEAARLGVNV---STTQVRSS 289 Query: 272 RAELDATAGDLFDMVLGNKISIEINQRYALKDVGQAH 308 A+L AG L V + + I+ +AL+D G+AH Sbjct: 290 GAQLQQLAGLLDSGV----VRVAIDSTFALQDAGKAH 322 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 80.5 bits (197), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 80/261 (30%), Positives = 118/261 (45%), Gaps = 26/261 (9%) Query: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAG----LYPQPLPA 58 +AI + G P V+ D++ G VR+ A G+ +D RAG L LP Sbjct: 2 RAICYHQYGPPSVLHLCDLDEQPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLPK 61 Query: 59 GLGQEAAGTIEAVGAGVSGFKVGDRV----AYAGRPNGAYAEERVMPADILVKLPDAISF 114 G++ AG I A+G GV +VG+ V + AGR G E V+P + +V+ PD +S Sbjct: 62 VPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGR--GTTLAELVLPQEQVVRKPDVLSM 119 Query: 115 DTAAAMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTV-SS 173 AAA++ + L QT + G + HA +G +G + Q A LG + + SS Sbjct: 120 QQAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSS 179 Query: 174 DEKAALALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGM 233 + + AL GA+ V+ Y + EL R VV D +G S LR G Sbjct: 180 NVEYVAAL--GASRVLAYDRD-------ELGRVRDQDVVIDLVGGAAHDQSYRMLRRGGQ 230 Query: 234 MVSFGNASGPV----APFGVS 250 +V + P+ FGVS Sbjct: 231 LVWL--VAAPIRERGEEFGVS 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,109,614 Number of extensions: 52036 Number of successful extensions: 195 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 6 Length of query: 324 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 239 Effective length of database: 1,217,395 Effective search space: 290957405 Effective search space used: 290957405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)