BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0005.p (393 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 806 0.0 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 129 3e-31 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 126 2e-30 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 111 8e-26 gi|300311139|ref|YP_003775231.1| MocR family transcription regul... 74 2e-14 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 806 bits (2082), Expect = 0.0, Method: Compositional matrix adjust. Identities = 393/393 (100%), Positives = 393/393 (100%) Query: 1 MNLNQLAARLQHIAPFHVMELSKKAAVLEQQGRHLIHMGIGEPDFTAPPAVVEAATRAMT 60 MNLNQLAARLQHIAPFHVMELSKKAAVLEQQGRHLIHMGIGEPDFTAPPAVVEAATRAMT Sbjct: 1 MNLNQLAARLQHIAPFHVMELSKKAAVLEQQGRHLIHMGIGEPDFTAPPAVVEAATRAMT 60 Query: 61 EGKMQYTSATGLPQLRAAISDHYRSVYGLEIAPERIVVTAGASAALLLACAALVERDSEV 120 EGKMQYTSATGLPQLRAAISDHYRSVYGLEIAPERIVVTAGASAALLLACAALVERDSEV Sbjct: 61 EGKMQYTSATGLPQLRAAISDHYRSVYGLEIAPERIVVTAGASAALLLACAALVERDSEV 120 Query: 121 LMPDPSYPCNRHFVAAFEGRAKLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTG 180 LMPDPSYPCNRHFVAAFEGRAKLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTG Sbjct: 121 LMPDPSYPCNRHFVAAFEGRAKLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTG 180 Query: 181 TSILPDELRAIVGEVRQRGGFTIVDEIYQGLSYEGAPFSALSLGDDVVVINSFSKYFNMT 240 TSILPDELRAIVGEVRQRGGFTIVDEIYQGLSYEGAPFSALSLGDDVVVINSFSKYFNMT Sbjct: 181 TSILPDELRAIVGEVRQRGGFTIVDEIYQGLSYEGAPFSALSLGDDVVVINSFSKYFNMT 240 Query: 241 GWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPQTLALYEERKAEFKRRRD 300 GWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPQTLALYEERKAEFKRRRD Sbjct: 241 GWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPQTLALYEERKAEFKRRRD 300 Query: 301 YIVPALESLGFTVPVMPDGAFYVYADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTA 360 YIVPALESLGFTVPVMPDGAFYVYADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTA Sbjct: 301 YIVPALESLGFTVPVMPDGAFYVYADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTA 360 Query: 361 RRYIRLSYATSMENLQEAVARLRVFFEKRRKAA 393 RRYIRLSYATSMENLQEAVARLRVFFEKRRKAA Sbjct: 361 RRYIRLSYATSMENLQEAVARLRVFFEKRRKAA 393 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 105/393 (26%), Positives = 188/393 (47%), Gaps = 21/393 (5%) Query: 9 RLQHIAPFHVMELSKKAAVLEQQGRHLIHMGIGEPDFTAPPAVVE----AATRAMTEGKM 64 R++ + P+ ++ ++G +I M +G PD PP +VE A R T G Sbjct: 43 RIERLPPYVFNITAELKMAARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHG-- 100 Query: 65 QYTSATGLPQLRAAISDHYRSVYGLEIAPE-RIVVTAGASAALLLACAALVERDSEVLMP 123 Y+S+ G+P+LR AI+ YRS Y ++ P+ +VT G+ L A +++ VL+P Sbjct: 101 -YSSSKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVP 159 Query: 124 DPSYPCNRH--FVAAFEGRAKLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTGT 181 +PSYP + + +A R+ ++ G + F + VRE + + ++L PSNPT Sbjct: 160 NPSYPIHIYGAVIAGANIRSVRMSPGVDF-FDELERAVRESY-PKPKMMILGFPSNPTAQ 217 Query: 182 SILPDELRAIVGEVRQRGGFTIVDEIYQGLSYEG--APFSALSLGDDVVVINSF--SKYF 237 + + +V R+ + D Y ++++G AP G V + F SK + Sbjct: 218 CVELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVAVEFFTLSKSY 277 Query: 238 NMTGWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPQTLALYEERKAEFKR 297 NM GWR+G++V LV + ++ + + Q AA+A + EE +A ++R Sbjct: 278 NMAGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQ-SCVEEIRANYER 336 Query: 298 RRDYIVPALESLGFTVPVMPDGAFYVYADCSALADDADQLSLD--MLNEAGVVLVPGLDF 355 RR+ +V L G+ V V P + Y++A L +L +A V + PG+ F Sbjct: 337 RRNVLVKGLHEAGWMVDV-PKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVSPGIGF 395 Query: 356 GPFTARRYIRLSYATSMENLQEAVARLRVFFEK 388 G + Y+R + + +++A+ ++ F+ Sbjct: 396 GEY-GDEYVRFALIENESRIRQAIRGIKAMFKN 427 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 105/367 (28%), Positives = 173/367 (47%), Gaps = 35/367 (9%) Query: 36 IHMGIGEPDFTAPPAVVEAATRAMTEGKMQYTSATGLPQLRAAISDHYRSVYGLEIAP-E 94 +++G G PDF PA+V A T+AM EG QY G+P LR AI++ +YG P Sbjct: 31 VNLGQGFPDFHCDPALVAAVTQAMQEGLNQYPPMAGVPVLREAIAEKVEKLYGHRYDPVS 90 Query: 95 RIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFEGRAKLIASGPEH-RFQ 153 I VTAGA+ +L + V EV++ +P Y C +V A E L P + + Sbjct: 91 EITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDC---YVPAIE----LAGGVPVFVQME 143 Query: 154 LSAQMVREHWGSA-------TRGVLLASPSNPTGTSILPDELRAIVGEVRQRGGFTIVDE 206 + AQ W TR +++ +P NPTG+ + ++ A+ VR + DE Sbjct: 144 VGAQGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDE 203 Query: 207 IYQGLSYEGAPFSALS----LGDDVVVINSFSKYFNMTGWRLGWLVLPPALVPQVEKLAQ 262 +Y+ + Y+G P +L+ L + + +SF K +++TGW++G++ P AL + K+ Q Sbjct: 204 VYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQ 263 Query: 263 NLLICASSIAQHAAVACFTPQTLALYEERKAEFKRRRDYIVPALESLGFTVPVMPDGAFY 322 + ++ Q+ A + A Y + A ++ +RD L + F ++P Y Sbjct: 264 FNVFTVNTPVQYGLAAYM--KDPAPYLDLPAFYQHKRDLFRTGLANTRFE--LLPSQGTY 319 Query: 323 V----YADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTARR---YIRLSYATSMENL 375 Y SAL + + + +E GV +P F + R +R +A E L Sbjct: 320 FQCVKYGAISALPE--AEFCKWLTSEIGVAAIPVSAF--YNTPRESGIVRFCFAKKDETL 375 Query: 376 QEAVARL 382 Q A+ RL Sbjct: 376 QLALERL 382 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 111 bits (278), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 99/387 (25%), Positives = 178/387 (45%), Gaps = 23/387 (5%) Query: 23 KKAAVLEQQGRHLIHMGIGEP---DFTAPPAVVEAATRAMTEGKMQYTSATGLPQLRAAI 79 +KA +E++G +I + IG F AP +V+ R M YT + GL R ++ Sbjct: 23 EKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNAS-GYTDSKGLFAPRKSV 81 Query: 80 SDHYRSVYGLE-IAPERIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFE 138 HY +E + + I + GAS ++++ AL+ EVL+P P YP V+ Sbjct: 82 M-HYTQQKKIEGVTIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSG 140 Query: 139 GRAKLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTGTSILPDELRAIVGEVRQR 198 G + +Q +++ T+ +++ +P+NPTG + L+ I+ RQ Sbjct: 141 GTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQH 200 Query: 199 GGFTIVDEIYQGLSYEGAPFSAL-SLGDDVVVI--NSFSKYFNMTGWRLGWLVLPPA--- 252 + DEIY + Y+G ++L SL DDV+ I N SK + G+R GW+V+ Sbjct: 201 QLIILADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKH 260 Query: 253 ---LVPQVEKLAQNLLICASSIAQHA-AVACFTPQTLALYEERKAEFKRRRDYIVPALES 308 + + LA ++ +CA++ Q A A Q++ + ++RD L Sbjct: 261 ARDYIEGLNMLA-SMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRDLAHQLLTD 319 Query: 309 LGFTVPVMPDGAFYVY----ADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTARRYI 364 + V P A Y++ + + DD + + ++L E V++V G F T + Sbjct: 320 IPGVSCVKPKSALYMFPRLDPEIYPIKDDQ-EFAYELLAEEKVLIVQGTGFNCPTPDHF- 377 Query: 365 RLSYATSMENLQEAVARLRVFFEKRRK 391 R+ + + ++L E++ R+ F E R+ Sbjct: 378 RVVFLPNTDDLTESMGRIAHFLEGYRR 404 >gi|300311139|ref|YP_003775231.1| MocR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 398 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 94/343 (27%), Positives = 148/343 (43%), Gaps = 39/343 (11%) Query: 61 EGKM--QYTSATGLPQLRAAISDHYRSVYGLEIAPERIVVTAGASAALLLACAALVERDS 118 +GKM QY G LR I+ S G +I+ E++++ +G+ L L L++ S Sbjct: 66 QGKMALQYGPTDGYLPLREWIAASL-STNGAQISAEQVLMVSGSQQGLDLLGKVLIDEGS 124 Query: 119 EVLMPDPSYPCNRHFVAAFEGRAKLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNP 178 +VL+ PSY A + + S P F L + + G A L + NP Sbjct: 125 KVLVETPSYLGALQAFALYGAK---FESVPSDEFGLQPETIEAIAGGARMLYSLPNFQNP 181 Query: 179 TGTSILPDELRAIVGEVRQRGGFTIV-DEIYQGLSYEGAPF-SALSLG-DDVVVINSFSK 235 TG + LP E R + E R G ++ D+ Y LSY+ AP LS+ V+ + SFSK Sbjct: 182 TGRT-LPTERRFKLVETCARLGLPLIEDDPYGALSYQNAPLPKMLSMNPSGVIYMGSFSK 240 Query: 236 YFNMTGWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPQTLALYEERKAEF 295 G RLG++V P L+ ++E+ Q + + + Q + +YE K F Sbjct: 241 VLT-PGIRLGYVVAPRPLILKMEQAKQATDLHTAQLTQ-----------MVVYEAIKDGF 288 Query: 296 ---------KRRRDYIVPALESLGFTVPV-----MPDGAFYVYADCSALADDADQLSLDM 341 K D L++L P P+G +++ DA L + Sbjct: 289 LDQHVPTIRKLYGDQCQAMLDALQQYFPASCSWSKPEGGMFIWVTLPEHI-DAGALLNEA 347 Query: 342 LNEAGVVLVPGLDFGPFTARR-YIRLSYAT-SMENLQEAVARL 382 + + V VPG F A++ +RLS+ T E ++ V RL Sbjct: 348 VEQEKVAFVPGAPFYANVAQKNTLRLSFVTVPPEQIRAGVERL 390 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,139,895 Number of extensions: 45017 Number of successful extensions: 185 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 8 Length of query: 393 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 306 Effective length of database: 1,207,925 Effective search space: 369625050 Effective search space used: 369625050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)