BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0361.p (629 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspir... 1148 0.0 gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspir... 107 4e-24 gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herb... 92 9e-20 gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein ... 85 1e-17 gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspir... 82 1e-16 >gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspirillum seropedicae SmR1] Length = 632 Score = 1148 bits (2969), Expect = 0.0, Method: Compositional matrix adjust. Identities = 620/632 (98%), Positives = 623/632 (98%), Gaps = 3/632 (0%) Query: 1 VSILTPASQGGGLPGQDASAAPFDPALLARLANELFAAPTAPAWTPGQAPGAQPPANFTP 60 +SILTPASQGGGLPGQDASAAPFDPALLARLANELFAAPTAPAWTPGQAPGAQPPANFTP Sbjct: 1 MSILTPASQGGGLPGQDASAAPFDPALLARLANELFAAPTAPAWTPGQAPGAQPPANFTP 60 Query: 61 PASPLSSPAGTSQGVPGTAIPQGLAPGLNLIPSSPHQVLTLGNRAPALAPAAPAGNGTPD 120 PASPLSSPAGTSQGVPGTAIPQGLAPGLNLIPSSPHQVLTLGNRAPALAPAAPAGNGTPD Sbjct: 61 PASPLSSPAGTSQGVPGTAIPQGLAPGLNLIPSSPHQVLTLGNRAPALAPAAPAGNGTPD 120 Query: 121 KVFAALPAYEPRLGGAVTGAPQAAAPSTPAAPSTAGYYFLDGGHGHPRGSAAAPAAAPAP 180 KVFAALPAYEPRLGGAVTGAPQAAAPSTPAAPSTAGYYFLDGGHGHPRGSAAAPAAAP Sbjct: 121 KVFAALPAYEPRLGGAVTGAPQAAAPSTPAAPSTAGYYFLDGGHGHPRGSAAAPAAAPLS 180 Query: 181 TGSASSP---QYYFLHAVELPSGYVTPAKPAPHAVHQPAGAQQAAAFDVHAVRRDFPILQ 237 + SA + QYYFLHAVELPSGYVTPAKPAPHAVHQPAGAQQAAAFDVH VRRDFPILQ Sbjct: 181 SSSAGAASSPQYYFLHAVELPSGYVTPAKPAPHAVHQPAGAQQAAAFDVHTVRRDFPILQ 240 Query: 238 ERVNGRPLIWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERV 297 ERVNGRPLIWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERV Sbjct: 241 ERVNGRPLIWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERV 300 Query: 298 RAFLNAPDVNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAE 357 RAFLNAPDVNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAE Sbjct: 301 RAFLNAPDVNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAE 360 Query: 358 KGAKLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGAR 417 KGAKLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGAR Sbjct: 361 KGAKLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGAR 420 Query: 418 TLVDGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRDVLEDMPPWQGGGNMI 477 TLVDGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRDVLEDMPPWQGGGNMI Sbjct: 421 TLVDGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRDVLEDMPPWQGGGNMI 480 Query: 478 ADVTFEKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRVGIDNIAAYEHALLEYGMHQL 537 ADVTFEKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRVGIDNIAAYEHALLEYGMHQL Sbjct: 481 ADVTFEKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRVGIDNIAAYEHALLEYGMHQL 540 Query: 538 GEIEGLRLIGTAAHKASVMSFVLEGYSTEDVGKALNQEGIAVRTGHHCAQPILRRFGVET 597 GEIEGLRLIGTAAHKASVMSFVLEGYSTEDVGKALNQEGIAVRTGHHCAQPILRRFGVET Sbjct: 541 GEIEGLRLIGTAAHKASVMSFVLEGYSTEDVGKALNQEGIAVRTGHHCAQPILRRFGVET 600 Query: 598 TVRPSLAFYNTFEEIDCLLTVVRRLAAQRHRR 629 TVRPSLAFYNTFEEIDCLLTVVRRLAAQRHRR Sbjct: 601 TVRPSLAFYNTFEEIDCLLTVVRRLAAQRHRR 632 >gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 107 bits (266), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 107/391 (27%), Positives = 169/391 (43%), Gaps = 39/391 (9%) Query: 246 IWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERVRAFLNAPD 305 I+ D +ATT V D++ + R + N +H A A E ARE V +NA D Sbjct: 20 IYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHMYGWTAEKAVEEAREEVAKLVNA-D 78 Query: 306 VNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAEKGAKLRVI 365 E+I+ G TE+ NL K + G II EH A + +++L G + + Sbjct: 79 SREIIWTSGATESNNLAIKGAAQFYKSKGKHIITVKTEHKAVLDTFRELE-RIGFEATYL 137 Query: 366 PVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGARTLVDGAQS 425 D+G V +++ A + T +V+V V+N +G I P +I E R G D AQ+ Sbjct: 138 QPQDNGLVTVEQIAAAVRPDTILVSVMLVNNEIGVIQPVPEIGEFCRSKGIIFHSDAAQA 197 Query: 426 VSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRD-VLEDMPPWQGGGNMIADVTFEK 484 ++ D++ D FS HK +GP GIGAL+ RR + GGG+ Sbjct: 198 TGKVKIDLENWKVDLMSFSAHKSYGPKGIGALYVRRKPRVRIEAQMHGGGH--------- 248 Query: 485 TIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRVGIDNIAAYEHALLEYGMHQLGEIEGLR 544 +GT VG+G A + R +D+ A AL + L EIE Sbjct: 249 ------ERGLRSGTLATHQIVGMGEAF-RLAREEMDSELARIRALRDRLAKGLQEIEETY 301 Query: 545 LIGTAAHKA----SVMSFVLEGYSTEDVGKALNQEGIAVRTGHHCAQP------ILRRFG 594 + G H+ +V +EG S + + IAV +G C +LR G Sbjct: 302 INGDMEHRVPHNLNVSFNYVEGESL-----IMAIKDIAVSSGSACTSASLEPSYVLRALG 356 Query: 595 -----VETTVRPSLAFYNTFEEIDCLLTVVR 620 +++R ++ + T E+ID + +++ Sbjct: 357 RSDELAHSSIRFTIGRFTTEEDIDFTIALIK 387 >gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herbaspirillum seropedicae SmR1] Length = 406 Score = 92.4 bits (228), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 104/393 (26%), Positives = 165/393 (41%), Gaps = 43/393 (10%) Query: 246 IWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERVRAFLNAPD 305 I+ D +ATT V ++ + N +H A A E ARE+V A +NA D Sbjct: 9 IYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVAALVNA-D 67 Query: 306 VNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAEKGAKLRVI 365 V E+I+ G TE+ NL K G +I EH A + ++L +G ++ + Sbjct: 68 VREIIWTSGATESNNLAIKGAAEFLRSKGRHLITVKTEHKAVLDTMRELE-RRGFEVTYL 126 Query: 366 PVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGARTLVDGAQS 425 V +G + L + L T + +V V+N +G I + ++ R G VD AQ+ Sbjct: 127 EVGSNGLLDLAAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLHVDAAQA 186 Query: 426 VSHMRTDVQALGADFFVFSGHKVFGPTGIGALW-GRRDVLEDMPPWQGGGNMIADVTFEK 484 + D+ L D FS HK +GP GIGAL+ GR+ + GGG+ Sbjct: 187 TGKVAIDLARLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGH--------- 237 Query: 485 TIFQPLPNTFEAGTGNIADAVGLGAAIDYVNR-VGIDNIAAYEHALLEYGMHQ-LGEIEG 542 +GT VG+G A + +G +N +L +H +IE Sbjct: 238 ------ERGLRSGTLATHQIVGMGEAFELARLAMGTEN---ERIRMLRDRLHAGFADIEE 288 Query: 543 LRLIGTAA----HKASVMSFVLEGYSTEDVGKALNQEGIAVRTGHHCAQP------ILRR 592 + G H +V +EG S + + +AV +G C +LR Sbjct: 289 CFVNGDLERRVPHNLNVSFNYVEGESL-----LMAIKDVAVSSGSACTSASLEPSYVLRA 343 Query: 593 FGVE-----TTVRPSLAFYNTFEEIDCLLTVVR 620 G+ +++R S+ + T EEID + V+R Sbjct: 344 LGMSDELAHSSIRFSIGRFTTVEEIDFTVNVIR 376 >gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein [Herbaspirillum seropedicae SmR1] Length = 379 Score = 85.1 bits (209), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 82/294 (27%), Positives = 131/294 (44%), Gaps = 36/294 (12%) Query: 337 IIVSHLEHHANIVPWQQLAAEKGAKLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSN 396 I+ S +EH + ++ + L A+ G ++ I VD+ GQ+ LDE A + T +V++ +N Sbjct: 93 IVSSLVEHPSTLLLLRHLQAQ-GVRISWIAVDEHGQLQLDELAAAITPDTALVSLMWANN 151 Query: 397 ALGTITPTKQIVELARLAGARTLVDGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGA 456 G + P Q ELAR G D Q+V + DV LG D S HK+ GP GIGA Sbjct: 152 ETGVVFPIAQAAELARQRGVLFHTDAVQAVGRVPCDVAGLGVDLLSLSAHKLHGPKGIGA 211 Query: 457 LWGRRDVLEDMPPWQGGGNMIADVTFEKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNR 516 L+ R+ + +PP +F GT ++A VGLG A Sbjct: 212 LYVRKGI--KLPPL---------------LFGHQERGRRGGTEDVAGIVGLGVAAAQARA 254 Query: 517 V--GIDNIAAYEHALLEYGMHQLGEIEGLRLIGT-AAHKASVMSFVLEGYSTEDVGKALN 573 + ++A E G+ +L ++ ++ G AA + +S E V + L+ Sbjct: 255 ALPRMQAVSALRQR-FERGLARLPQV---KINGAGAARLPNTVSVRFGQVHAELVLERLD 310 Query: 574 QEGIAVRTGHHCAQP------ILRRFGVET-----TVRPSLAFYNTFEEIDCLL 616 + GI +G C +L G+ + T+R SL+ +EID +L Sbjct: 311 RLGICASSGSACTASGDMPSHVLMAMGMSSEQAVATIRFSLSRDTREDEIDAVL 364 >gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 82.4 bits (202), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 107/398 (26%), Positives = 165/398 (41%), Gaps = 41/398 (10%) Query: 246 IWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERVRAFLNAPD 305 I+ D ATT V + +F + N ++H A A E AR +V + A Sbjct: 17 IYLDYGATTPVDPQVARVMCDFLTDKFGNAASSSHPFGWEARRAVEQARAQVAGLVGAQP 76 Query: 306 VNEVIFVRGTTEAINLVAK--SWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAEKGAKLR 363 E+++ G TE+ NL K ++ + G G+ +I EH A + L + G ++ Sbjct: 77 -GEIVWTSGATESNNLALKGAAYALRAQGRGNHLITVPTEHKAVLDTMHTLE-KAGFEVT 134 Query: 364 VIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGARTLVDGA 423 + G + + L T + +V V+N +G I P ++ E+ VD Sbjct: 135 YLQPGADGLITPQQLRAALRPDTLLASVMYVNNEIGVIQPIAELGEICAAHDVVFHVDAV 194 Query: 424 QSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRD-VLEDMPPWQGGGNMIADVTF 482 Q+ + D+ L D FS HKV+GP GIGAL+ R + L+ GGG+ Sbjct: 195 QAAGKIAIDLTRLKVDLMSFSAHKVYGPKGIGALYVRHNPQLKLEAQIDGGGH------- 247 Query: 483 EKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRVGIDNIAAYEHALLEYGMHQLGEIEG 542 N +GT VG+G A R+ ++ + AL + L ++ G Sbjct: 248 --------ENGMRSGTLATHQIVGMGEAFALAGRL-LEEESRRIGALRDRLWAGLADLPG 298 Query: 543 LRLIGTAA----HKASVMSFVLEGYSTEDVGKALNQEGIAVRTGHHC----AQP--ILRR 592 ++L G A H +V SF + T +G L GIAV G C A P +L Sbjct: 299 VQLNGCARQRVPHNLNV-SFPPTRHGT--LGSQL--LGIAVSAGSACNSAAAAPSFVLLA 353 Query: 593 FG-----VETTVRPSLAFYNTFEEIDCLLTVVRRLAAQ 625 G VR SL T EID ++ VR++ A Sbjct: 354 IGRSPDVARNAVRFSLGRGTTEAEIDYVVATVRQVLAH 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,903,620 Number of extensions: 79789 Number of successful extensions: 347 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 343 Number of HSP's successfully gapped: 5 Length of query: 629 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 538 Effective length of database: 1,188,985 Effective search space: 639673930 Effective search space used: 639673930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)