BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0354.p (1132 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311640|ref|YP_003775732.1| alpha-amylase [Herbaspirillum s... 2250 0.0 >gi|300311640|ref|YP_003775732.1| alpha-amylase [Herbaspirillum seropedicae SmR1] Length = 1132 Score = 2250 bits (5831), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1106/1132 (97%), Positives = 1111/1132 (98%) Query: 1 LENLPLTDFSPRIYCVDPSLVASPDWDDLLGRCAAMGFDHVLLCGDRVDASLAEVAKLCK 60 +ENLPLTDFSPRIYCVDPSLVASPDWDDLLGRCAAMGFDHVLLCGD VDAS AEVAKLCK Sbjct: 1 MENLPLTDFSPRIYCVDPSLVASPDWDDLLGRCAAMGFDHVLLCGDLVDASFAEVAKLCK 60 Query: 61 GHRLRLLVDLSLTRFYAGDPMLTSHADWFAPQSSAAAGLSSQDLASDPLPDPRYPSVMDE 120 GHRLRLL DLSLTRFYAGDP+LTSHADWFAPQSSAA GLSSQDLASDPLPDPRYPSVMDE Sbjct: 61 GHRLRLLADLSLTRFYAGDPVLTSHADWFAPQSSAAVGLSSQDLASDPLPDPRYPSVMDE 120 Query: 121 REDEFVTRRPRFDDPVAADAMLDWWRARLMTLVDAGVAGFRCQEPASIPADFWRGLIAGI 180 R+DE VTRR RFDDPVAADAMLDWWRARLMTLVDAGVAGFRCQEPASIPADFWR LIAGI Sbjct: 121 RDDELVTRRARFDDPVAADAMLDWWRARLMTLVDAGVAGFRCQEPASIPADFWRRLIAGI 180 Query: 181 RQQQADCRFLAWTPGCTHDQLDGLAGIVFDAVFSSGAWWDFRDPWYFREYRKLAAIAPVL 240 RQQQADCRFLAWTPGCTHDQLDGLAGI FDAVFSSGAWWDFRDPWYFREYRKLAAIAPVL Sbjct: 181 RQQQADCRFLAWTPGCTHDQLDGLAGIGFDAVFSSGAWWDFRDPWYFREYRKLAAIAPVL 240 Query: 241 AFPEDSSGPRLARVHGFRDPQTRRLGALRALQFATLSAVGWMMPMGFEFGLTAALRVAHR 300 AFPED SGPRLARVHGFRDPQTRRLGALRALQFATLSA GWMMPMGFEFGLTAALRVAHR Sbjct: 241 AFPEDPSGPRLARVHGFRDPQTRRLGALRALQFATLSAEGWMMPMGFEFGLTAALRVAHR 300 Query: 301 AGSATRLSDPEAFKRACADADLDFTREITEANRTLAQRRQSPPKSLALRLTPLSAPDSPW 360 AGSATRLSDPEAFKRACADADLDFTREITEANRTLAQRRQSPPKSLALRLTPLSAPDSPW Sbjct: 301 AGSATRLSDPEAFKRACADADLDFTREITEANRTLAQRRQSPPKSLALRLTPLSAPDSPW 360 Query: 361 LLMERHGVNAEDGLLIAVNPALDRPVRITDDGWQQRLQEGVAPWQEDGLVLEPGAVKILP 420 LLMERHGVN EDGLLIAVNPALDRPVRITDDGWQQRLQE VAPWQEDGLVLEPGAVK+LP Sbjct: 361 LLMERHGVNVEDGLLIAVNPALDRPVRITDDGWQQRLQESVAPWQEDGLVLEPGAVKVLP 420 Query: 421 LKPLQGVSGELDQAIPAPGDMRSRRIAIERVSPAVDDGAFAAKRIVGEHFVIAADIFMDG 480 LKPLQGVSGELDQA PA GDMRSRRIAIERVSPAVDDGAFAAKRIVGEHFVIAADIFMDG Sbjct: 421 LKPLQGVSGELDQATPALGDMRSRRIAIERVSPAVDDGAFAAKRIVGEHFVIAADIFMDG 480 Query: 481 HEHLAAEVLVRAADEAQWRRIPMHPDVNDRWQARVSLTRLGRHYFCIEAWSDVFDTFRDG 540 H+HLAAEVLVRAADEAQWRRIPMHPDVNDRWQARVSLTRLGRHYFCIEAWSDVFDTFRDG Sbjct: 481 HDHLAAEVLVRAADEAQWRRIPMHPDVNDRWQARVSLTRLGRHYFCIEAWSDVFDTFRDG 540 Query: 541 LQKKLMAGQDVSLEMEEGRLLIARLVQRAVEQELEPSVLESSMALLKALGGPPSKTRRSA 600 LQKKLMAGQDVSLEMEEGRLLIARLVQRAVEQELEPSVLESS ALLKALGGPPSKTRRSA Sbjct: 541 LQKKLMAGQDVSLEMEEGRLLIARLVQRAVEQELEPSVLESSRALLKALGGPPSKTRRSA 600 Query: 601 ASKQADTDKRLAFLCAEETRQLVEQLTGLAGERAFLSRTDAEYPLEAERRSAAFSSWYEL 660 A KQADTDKRLAFLCAEETRQLVEQLTGLAGERAFLSRTDAEYPLEAERRSAAFSSWYEL Sbjct: 601 AGKQADTDKRLAFLCAEETRQLVEQLTGLAGERAFLSRTDAEYPLEAERRSAAFSSWYEL 660 Query: 661 FPRSQSGSADVHGNFDDVITRLPAIRAMGFDTLYFPPIHPIGKKHRKGKNNSVTAGPDDP 720 FPRSQSGSADVHGNFDDVI RLPAIRAMGFDTLYFPPIHPIGKKHRKGKNNSVTAGPDDP Sbjct: 661 FPRSQSGSADVHGNFDDVIARLPAIRAMGFDTLYFPPIHPIGKKHRKGKNNSVTAGPDDP 720 Query: 721 GSPYAIGSEEGGHDAIHPQLGTFEDFARLRDAAAAHGLELALDFAIQCSPDHPWLKTHPG 780 GSPYAIGSEEGGHDAIHPQLGTFEDFARLRDAAAAHGLELALDFAIQCSPDHPWLKTHPG Sbjct: 721 GSPYAIGSEEGGHDAIHPQLGTFEDFARLRDAAAAHGLELALDFAIQCSPDHPWLKTHPG 780 Query: 781 WFAWRPDGSMRYAENPPKKYQDIVNVDFYAEDAIPELWLALRDIVVFWVQQGVSVFRVDN 840 WFAWRPDGSMRYAENPPKKYQDIVNVDFYAEDAIPELWLALRDIVVFWVQQGVSVFRVDN Sbjct: 781 WFAWRPDGSMRYAENPPKKYQDIVNVDFYAEDAIPELWLALRDIVVFWVQQGVSVFRVDN 840 Query: 841 PHTKPFPFWEWMIASVRSRYPQVIFLSEAFTRPKPMYRLAKVGFSQSYTYFTWRHGKQEL 900 PHTKPFPFWEWMIASVRSRYPQVIFLSEAFTRPKPMYRLAKVGFSQSYTYFTWRHGKQEL Sbjct: 841 PHTKPFPFWEWMIASVRSRYPQVIFLSEAFTRPKPMYRLAKVGFSQSYTYFTWRHGKQEL 900 Query: 901 IDYLTELTTDTGHAAQPRDFFRPHFFVNTPDINPCFLQRSGRAGFQIRAALAATLSGLWG 960 IDYLTELTTDTGHAAQPRDFFRPHFFVNTPDINP FLQRSGRAGFQIRAALAATLSGLWG Sbjct: 901 IDYLTELTTDTGHAAQPRDFFRPHFFVNTPDINPYFLQRSGRAGFQIRAALAATLSGLWG 960 Query: 961 VYSGFELGEAKAVPGKEEYLDSEKYEIRSWDWQRPGNIIEDITRLNRIRTENPALQTHLG 1020 VYSGFELGEA AVPGKEEYLDSEKYEIRSWDWQRPGNIIEDITRLNRIRTENPALQTHLG Sbjct: 961 VYSGFELGEANAVPGKEEYLDSEKYEIRSWDWQRPGNIIEDITRLNRIRTENPALQTHLG 1020 Query: 1021 VHFLAVSNDQILYFVKSTPAAPSGRAAVEGGLRFGDNTILVAINLDPFQSQQGEIELPLA 1080 V FL VSNDQILYFVKSTPAAPSGRAAVEGGLRFGDNTILVAINLDPFQSQQGEIELPLA Sbjct: 1021 VRFLPVSNDQILYFVKSTPAAPSGRAAVEGGLRFGDNTILVAINLDPFQSQQGEIELPLA 1080 Query: 1081 GFGLPEGAEPAHLEVVDLLGEDRFKWYGRHQSITLDPRERPYRIWRIRAGET 1132 GFGLPEGAEPAHLEVVDLLGEDRFKWYGRHQSITLDPRERPYRIWRIRAGET Sbjct: 1081 GFGLPEGAEPAHLEVVDLLGEDRFKWYGRHQSITLDPRERPYRIWRIRAGET 1132 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,018,600 Number of extensions: 187139 Number of successful extensions: 497 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 492 Number of HSP's successfully gapped: 1 Length of query: 1132 Length of database: 1,619,870 Length adjustment: 96 Effective length of query: 1036 Effective length of database: 1,165,310 Effective search space: 1207261160 Effective search space used: 1207261160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 136 (57.0 bits)