BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0327.p (643 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311615|ref|YP_003775707.1| outer membrane biogenesis prote... 1248 0.0 gi|300314078|ref|YP_003778170.1| uncharacterized protein involve... 376 e-105 >gi|300311615|ref|YP_003775707.1| outer membrane biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 654 Score = 1248 bits (3229), Expect = 0.0, Method: Compositional matrix adjust. Identities = 633/643 (98%), Positives = 636/643 (98%) Query: 1 MKLLGRALAALAALLVALLLALALAVPLLDWNSAKPWLGRQLSAALGRDIRVDGNLDLSW 60 MKLLGRALAALAALLVALLLALALAVPLLDWNSAKPWLGRQLSAALGRDIRVDGNLDLSW Sbjct: 12 MKLLGRALAALAALLVALLLALALAVPLLDWNSAKPWLGRQLSAALGRDIRVDGNLDLSW 71 Query: 61 SLAPADDEASTLARLAPQLRLRAERLFIGNPDWAHSSDHLVRAAALDVRFSPLPLLHWHW 120 SLA ADDEA LARLAPQLRLRAERLFIGNPDWAHSSDHLVRAAALDVRFSPLPLLHWHW Sbjct: 72 SLAQADDEAGALARLAPQLRLRAERLFIGNPDWAHSSDHLVRAAALDVRFSPLPLLHWHW 131 Query: 121 HVTDLRVDGLDLVMERADDQRKNWRFSSQPRSRWSFDIHRIVFGHALIRYVDLPLDLDLR 180 HVTDLRV+GLDLVMERADDQRKNWRFSSQPRSRWSFDIHRIVFGHALIRYV+LPLDLDLR Sbjct: 132 HVTDLRVEGLDLVMERADDQRKNWRFSSQPRSRWSFDIHRIVFGHALIRYVELPLDLDLR 191 Query: 181 FDARPLNPTATNQKDSAALVLQAAVTGRYGQAEVNGQIKGGALPDLLNEGSVFPLQAEGD 240 FDARPLN TATNQKDSAALVLQAAVTGRYGQAEVNGQIKGGALPDLLNEGSVFPLQAEGD Sbjct: 192 FDARPLNATATNQKDSAALVLQAAVTGRYGQAEVNGQIKGGALPDLLNEGSVFPLQAEGD 251 Query: 241 IGGVQTQLSGNLINPHQLSRADLRIGLAGDSLDRLYPATGVPLPATRAFRTEGALTITRL 300 IGGVQTQLSGNLINPHQLSRADLRIGLAGDSLDRLYPATGVPLPATRAF TEGALTITRL Sbjct: 252 IGGVQTQLSGNLINPHQLSRADLRIGLAGDSLDRLYPATGVPLPATRAFHTEGALTITRL 311 Query: 301 DPQTKAWDWRYSGFSGKVGESDFAGDAQYVRGPPKNHLKVKAEAGLLRLSDYLPGDDADQ 360 DPQTKAWDWRYSGFSGKVGESDFAGDAQYVRGPPKNHLKVKAEAGLLRLSDYLPGDDADQ Sbjct: 312 DPQTKAWDWRYSGFSGKVGESDFAGDAQYVRGPPKNHLKVKAEAGLLRLSDYLPGDDADQ 371 Query: 361 GKPSKPAQPERWSKLDADIDLQAKRVQWRAGLEWQQAAATLTLKDQVLTADPLRFNMAGG 420 GKPSKPAQPERWSKLDADIDLQAKRVQWRAGLEWQQAAATLTLKDQVLTADPLRFNMAGG Sbjct: 372 GKPSKPAQPERWSKLDADIDLQAKRVQWRAGLEWQQAAATLTLKDQVLTADPLRFNMAGG 431 Query: 421 QGQGQVRLDGRQPDIRSRLQIRLQDAQVRELFPRLASLKASFSKLNGEATLSATGNSAAA 480 QGQGQVRLDGRQPDIRSRLQIRLQDAQVRELFPRLASLKASFSK NGEATLSATGNSAAA Sbjct: 432 QGQGQVRLDGRQPDIRSRLQIRLQDAQVRELFPRLASLKASFSKFNGEATLSATGNSAAA 491 Query: 481 VLASADGEIKADLGPGTISQFILEAAGLNLANAVFAKLYRDQQVKLLCGAADVAIQHGQA 540 VLASADGEIKADLGPGTISQFILEAAGLNLANAVFAKLYRDQQVKLLCGAADVAIQHGQA Sbjct: 492 VLASADGEIKADLGPGTISQFILEAAGLNLANAVFAKLYRDQQVKLLCGAADVAIQHGQA 551 Query: 541 TIRHGILNTEDAAIDVGGQVDLGRQTLALDIHPRTKQVRILSLRTPLHVRGSFSHPEIGA 600 TIRHGILNTEDAAIDVGGQVDLGRQTLALDIHPRTKQVRILSLRTPLHVRGSFSHPEIGA Sbjct: 552 TIRHGILNTEDAAIDVGGQVDLGRQTLALDIHPRTKQVRILSLRTPLHVRGSFSHPEIGA 611 Query: 601 DSGALAARAGAAAALALVAPVAAVIPLISPGQAAPDDCAARSR 643 DSGALAARAGAAAALALVAPVAAVIPLISPGQAA DDCAAR+R Sbjct: 612 DSGALAARAGAAAALALVAPVAAVIPLISPGQAASDDCAARAR 654 >gi|300314078|ref|YP_003778170.1| uncharacterized protein involved in outer membrane biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 682 Score = 376 bits (966), Expect = e-105, Method: Compositional matrix adjust. Identities = 244/663 (36%), Positives = 357/663 (53%), Gaps = 32/663 (4%) Query: 1 MKLLGRALAALAALLVALLLALALAVPLLDWNSAKPWLGRQLSAALGRDIRVDGNLDLSW 60 M L + + L A L+ L+ A + + DWN AKPW+ ++SA++GR+ +DG+L L W Sbjct: 1 MPRLTKIILWLCASLILLITAAVIFILSFDWNRAKPWINERVSASIGREFAIDGDLSLHW 60 Query: 61 SLA---PADDEASTLARLAPQLRLRAERLFIGNPDWAHSSDHLVRAAALDVRFSPLPLLH 117 A P R P RL+AE+L IGNP + + + +PLPLL Sbjct: 61 QRAQTLPGAAPPGFWERWLPWPRLQAEQLRIGNPPAFSQAGQMATIGQVTFTLNPLPLLV 120 Query: 118 WHWHVTDLRVDGLDLVMERADDQRKNWRF--SSQPRSRWSFDIHRIVFGHALIRYVDLPL 175 + L + + + R NW F SS S W+ + RI + D Sbjct: 121 HDIALPSLDIRDPSVSLLRNAQAVNNWTFKSSSAGPSAWNLRLGRITLRQGKLSLDDAVD 180 Query: 176 DLDLRFDARPLNPTATNQKDSAALVLQAAVTGRYGQAEVNGQIKGGALPDLLNEGSVFPL 235 + L+ + L+P L ++G + A V+GQ K G + L N +PL Sbjct: 181 KIKLKAEIDTLDPAVEKIYG-----LGWKLSGSFNGAPVSGQGKAGGVLSLQNVQQPYPL 235 Query: 236 QAEGDIGGVQTQLSGNLINPHQLSRADLRIGLAGDSLDRLYPATGVPLPATRAFRTEGAL 295 QA+ +G + G L P L+ DLR+ ++G S+ LYP TGV LP T F TEG L Sbjct: 236 QAQVRVGKTAIGVHGTLTRPAALAALDLRLSVSGASMGNLYPLTGVLLPETPPFATEGRL 295 Query: 296 TITRLDPQTKAWDWRYSGFSGKVGESDFAGDAQYVRGP--PKNHLKVKAEAGLLRLSDYL 353 I ++D WD Y F GKVG+SD G +Y G ++ L + +LR D Sbjct: 296 -IGKIDAGHSQWD--YEDFRGKVGQSDINGSLRYTTGAGAARSQLTGSLTSEVLRFDDLA 352 Query: 354 P--GDDADQGK---------------PSKPAQPERWSKLDADIDLQAKRVQWRAGLEWQQ 396 P G D++ K P K + W LDAD+ L KR+ + L Sbjct: 353 PLIGADSNAEKAERGAQERQPGDKVLPVKTFSTDAWGALDADVKLTGKRIIKQKNLPISD 412 Query: 397 AAATLTLKDQVLTADPLRFNMAGGQGQGQVRLDGRQPDIRSRLQIRLQDAQVRELFPRLA 456 A L LKD+VLT PL F +AGG + +RLDG+ ++S L + + ++++L+P+L Sbjct: 413 LQAHLILKDKVLTLAPLDFGVAGGNLRSTIRLDGQAAPMKSELILSARRLKIQKLYPQLD 472 Query: 457 SLKASFSKLNGEATLSATGNSAAAVLASADGEIKADLGPGTISQFILEAAGLNLANAVFA 516 + + SF ++NG+A LSATGNS AA+L S++GE+K + G IS+F+LEAAGLNL N V + Sbjct: 473 ASRTSFGEINGDARLSATGNSVAAMLGSSNGEVKTLVTQGAISKFLLEAAGLNLGNVVIS 532 Query: 517 KLYRDQQVKLLCGAADVAIQHGQATIRHGILNTEDAAIDVGGQVDLGRQTLALDIHPRTK 576 KL+ D++VKL C A D+ ++ G T R +L+TEDA I V G +D+ R+ L L +HP++K Sbjct: 533 KLFGDKEVKLNCLAGDLPVREGIMTARTLMLDTEDALISVDGTIDMRREKLDLHVHPKSK 592 Query: 577 QVRILSLRTPLHVRGSFSHPEIGADSGALAARAGAAAALALVAPVAAVIPLISPGQAAPD 636 +RI+SLR+PL+V G+F P++G D G LA RAG+A AL L+APV A++PLI+ Sbjct: 593 GLRIISLRSPLYVAGTFKQPDVGVDKGMLALRAGSAVALGLLAPVTAILPLINVDGDEQA 652 Query: 637 DCA 639 DCA Sbjct: 653 DCA 655 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,988,254 Number of extensions: 86460 Number of successful extensions: 308 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 304 Number of HSP's successfully gapped: 2 Length of query: 643 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 552 Effective length of database: 1,188,985 Effective search space: 656319720 Effective search space used: 656319720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)